Amino acid dipepetide frequency for Rhodococcus phage E3

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.47AlaAla: 12.47 ± 0.799
0.73AlaCys: 0.73 ± 0.122
7.113AlaAsp: 7.113 ± 0.417
6.93AlaGlu: 6.93 ± 0.461
2.417AlaPhe: 2.417 ± 0.219
6.839AlaGly: 6.839 ± 0.448
2.211AlaHis: 2.211 ± 0.19
4.24AlaIle: 4.24 ± 0.325
4.924AlaLys: 4.924 ± 0.419
7.956AlaLeu: 7.956 ± 0.447
2.895AlaMet: 2.895 ± 0.243
3.579AlaAsn: 3.579 ± 0.304
4.445AlaPro: 4.445 ± 0.425
4.217AlaGln: 4.217 ± 0.381
7.66AlaArg: 7.66 ± 0.481
6.315AlaSer: 6.315 ± 0.341
6.178AlaThr: 6.178 ± 0.403
7.158AlaVal: 7.158 ± 0.432
1.368AlaTrp: 1.368 ± 0.187
2.667AlaTyr: 2.667 ± 0.26
0.0AlaXaa: 0.0 ± 0.0
Cys
0.502CysAla: 0.502 ± 0.11
0.068CysCys: 0.068 ± 0.037
0.661CysAsp: 0.661 ± 0.136
0.502CysGlu: 0.502 ± 0.127
0.182CysPhe: 0.182 ± 0.065
1.254CysGly: 1.254 ± 0.202
0.274CysHis: 0.274 ± 0.097
0.296CysIle: 0.296 ± 0.088
0.296CysLys: 0.296 ± 0.088
0.57CysLeu: 0.57 ± 0.112
0.091CysMet: 0.091 ± 0.044
0.319CysAsn: 0.319 ± 0.078
0.524CysPro: 0.524 ± 0.177
0.182CysGln: 0.182 ± 0.07
0.707CysArg: 0.707 ± 0.133
0.707CysSer: 0.707 ± 0.139
0.752CysThr: 0.752 ± 0.129
0.456CysVal: 0.456 ± 0.099
0.251CysTrp: 0.251 ± 0.073
0.296CysTyr: 0.296 ± 0.086
0.0CysXaa: 0.0 ± 0.0
Asp
6.497AspAla: 6.497 ± 0.351
0.889AspCys: 0.889 ± 0.17
4.263AspAsp: 4.263 ± 0.322
5.107AspGlu: 5.107 ± 0.458
1.961AspPhe: 1.961 ± 0.242
5.38AspGly: 5.38 ± 0.403
1.641AspHis: 1.641 ± 0.231
2.827AspIle: 2.827 ± 0.24
1.938AspLys: 1.938 ± 0.198
5.198AspLeu: 5.198 ± 0.335
1.55AspMet: 1.55 ± 0.21
1.847AspAsn: 1.847 ± 0.252
4.468AspPro: 4.468 ± 0.349
2.713AspGln: 2.713 ± 0.258
4.468AspArg: 4.468 ± 0.365
3.556AspSer: 3.556 ± 0.312
2.872AspThr: 2.872 ± 0.23
5.357AspVal: 5.357 ± 0.389
1.163AspTrp: 1.163 ± 0.145
2.075AspTyr: 2.075 ± 0.232
0.0AspXaa: 0.0 ± 0.0
Glu
6.725GluAla: 6.725 ± 0.487
0.57GluCys: 0.57 ± 0.136
3.876GluAsp: 3.876 ± 0.464
3.967GluGlu: 3.967 ± 0.389
1.733GluPhe: 1.733 ± 0.226
4.537GluGly: 4.537 ± 0.36
1.778GluHis: 1.778 ± 0.194
3.716GluIle: 3.716 ± 0.277
2.758GluLys: 2.758 ± 0.296
6.155GluLeu: 6.155 ± 0.492
1.847GluMet: 1.847 ± 0.207
1.619GluAsn: 1.619 ± 0.166
2.827GluPro: 2.827 ± 0.252
2.941GluGln: 2.941 ± 0.289
5.449GluArg: 5.449 ± 0.453
3.853GluSer: 3.853 ± 0.363
2.872GluThr: 2.872 ± 0.261
4.673GluVal: 4.673 ± 0.359
1.322GluTrp: 1.322 ± 0.168
1.915GluTyr: 1.915 ± 0.201
0.0GluXaa: 0.0 ± 0.0
Phe
2.644PheAla: 2.644 ± 0.261
0.228PheCys: 0.228 ± 0.077
2.553PheAsp: 2.553 ± 0.229
1.892PheGlu: 1.892 ± 0.185
0.707PhePhe: 0.707 ± 0.129
2.485PheGly: 2.485 ± 0.255
0.661PheHis: 0.661 ± 0.121
0.752PheIle: 0.752 ± 0.133
1.003PheLys: 1.003 ± 0.171
1.869PheLeu: 1.869 ± 0.193
0.638PheMet: 0.638 ± 0.132
0.912PheAsn: 0.912 ± 0.126
1.505PhePro: 1.505 ± 0.21
1.14PheGln: 1.14 ± 0.164
1.527PheArg: 1.527 ± 0.195
1.915PheSer: 1.915 ± 0.197
1.961PheThr: 1.961 ± 0.192
1.961PheVal: 1.961 ± 0.237
0.388PheTrp: 0.388 ± 0.09
0.752PheTyr: 0.752 ± 0.142
0.0PheXaa: 0.0 ± 0.0
Gly
6.155GlyAla: 6.155 ± 0.518
0.821GlyCys: 0.821 ± 0.139
4.787GlyAsp: 4.787 ± 0.275
5.175GlyGlu: 5.175 ± 0.333
2.303GlyPhe: 2.303 ± 0.209
7.227GlyGly: 7.227 ± 0.675
1.938GlyHis: 1.938 ± 0.196
3.762GlyIle: 3.762 ± 0.301
3.465GlyLys: 3.465 ± 0.298
6.041GlyLeu: 6.041 ± 0.405
2.417GlyMet: 2.417 ± 0.253
2.576GlyAsn: 2.576 ± 0.327
3.625GlyPro: 3.625 ± 0.319
3.237GlyGln: 3.237 ± 0.298
4.81GlyArg: 4.81 ± 0.409
4.924GlySer: 4.924 ± 0.407
5.631GlyThr: 5.631 ± 0.389
6.087GlyVal: 6.087 ± 0.387
1.915GlyTrp: 1.915 ± 0.218
3.26GlyTyr: 3.26 ± 0.406
0.0GlyXaa: 0.0 ± 0.0
His
1.961HisAla: 1.961 ± 0.233
0.114HisCys: 0.114 ± 0.044
1.755HisAsp: 1.755 ± 0.234
1.733HisGlu: 1.733 ± 0.22
0.707HisPhe: 0.707 ± 0.137
1.778HisGly: 1.778 ± 0.185
0.798HisHis: 0.798 ± 0.141
1.003HisIle: 1.003 ± 0.157
0.57HisLys: 0.57 ± 0.117
2.097HisLeu: 2.097 ± 0.259
0.365HisMet: 0.365 ± 0.101
0.616HisAsn: 0.616 ± 0.11
1.459HisPro: 1.459 ± 0.256
0.843HisGln: 0.843 ± 0.151
1.368HisArg: 1.368 ± 0.172
1.299HisSer: 1.299 ± 0.179
1.208HisThr: 1.208 ± 0.155
1.55HisVal: 1.55 ± 0.196
0.502HisTrp: 0.502 ± 0.105
0.866HisTyr: 0.866 ± 0.144
0.0HisXaa: 0.0 ± 0.0
Ile
5.54IleAla: 5.54 ± 0.355
0.342IleCys: 0.342 ± 0.074
3.078IleAsp: 3.078 ± 0.291
3.351IleGlu: 3.351 ± 0.282
1.071IlePhe: 1.071 ± 0.164
4.195IleGly: 4.195 ± 0.376
0.843IleHis: 0.843 ± 0.141
1.664IleIle: 1.664 ± 0.219
1.824IleLys: 1.824 ± 0.219
2.508IleLeu: 2.508 ± 0.299
0.638IleMet: 0.638 ± 0.118
1.505IleAsn: 1.505 ± 0.215
2.303IlePro: 2.303 ± 0.216
1.55IleGln: 1.55 ± 0.203
2.895IleArg: 2.895 ± 0.226
2.713IleSer: 2.713 ± 0.266
3.488IleThr: 3.488 ± 0.301
3.693IleVal: 3.693 ± 0.286
0.502IleTrp: 0.502 ± 0.101
1.436IleTyr: 1.436 ± 0.15
0.0IleXaa: 0.0 ± 0.0
Lys
4.491LysAla: 4.491 ± 0.311
0.228LysCys: 0.228 ± 0.081
2.052LysAsp: 2.052 ± 0.207
1.801LysGlu: 1.801 ± 0.22
0.935LysPhe: 0.935 ± 0.172
2.348LysGly: 2.348 ± 0.233
0.866LysHis: 0.866 ± 0.128
1.824LysIle: 1.824 ± 0.19
1.14LysLys: 1.14 ± 0.195
3.442LysLeu: 3.442 ± 0.31
1.071LysMet: 1.071 ± 0.162
0.775LysAsn: 0.775 ± 0.154
2.12LysPro: 2.12 ± 0.249
1.254LysGln: 1.254 ± 0.201
2.804LysArg: 2.804 ± 0.249
2.257LysSer: 2.257 ± 0.267
2.553LysThr: 2.553 ± 0.283
3.237LysVal: 3.237 ± 0.304
0.502LysTrp: 0.502 ± 0.109
1.368LysTyr: 1.368 ± 0.161
0.0LysXaa: 0.0 ± 0.0
Leu
8.367LeuAla: 8.367 ± 0.487
0.684LeuCys: 0.684 ± 0.134
5.517LeuAsp: 5.517 ± 0.383
5.654LeuGlu: 5.654 ± 0.427
1.778LeuPhe: 1.778 ± 0.276
5.699LeuGly: 5.699 ± 0.433
1.938LeuHis: 1.938 ± 0.218
3.146LeuIle: 3.146 ± 0.255
2.804LeuLys: 2.804 ± 0.21
5.608LeuLeu: 5.608 ± 0.425
1.961LeuMet: 1.961 ± 0.238
2.143LeuAsn: 2.143 ± 0.24
4.377LeuPro: 4.377 ± 0.31
2.85LeuGln: 2.85 ± 0.26
6.566LeuArg: 6.566 ± 0.419
4.856LeuSer: 4.856 ± 0.326
5.517LeuThr: 5.517 ± 0.398
5.745LeuVal: 5.745 ± 0.43
0.866LeuTrp: 0.866 ± 0.132
2.052LeuTyr: 2.052 ± 0.227
0.0LeuXaa: 0.0 ± 0.0
Met
2.736MetAla: 2.736 ± 0.237
0.251MetCys: 0.251 ± 0.075
1.14MetAsp: 1.14 ± 0.163
0.98MetGlu: 0.98 ± 0.162
0.935MetPhe: 0.935 ± 0.177
1.596MetGly: 1.596 ± 0.203
0.593MetHis: 0.593 ± 0.115
0.935MetIle: 0.935 ± 0.13
0.866MetLys: 0.866 ± 0.155
1.596MetLeu: 1.596 ± 0.185
0.684MetMet: 0.684 ± 0.145
0.775MetAsn: 0.775 ± 0.111
1.892MetPro: 1.892 ± 0.23
1.049MetGln: 1.049 ± 0.192
1.664MetArg: 1.664 ± 0.207
2.417MetSer: 2.417 ± 0.255
2.85MetThr: 2.85 ± 0.206
1.231MetVal: 1.231 ± 0.182
0.182MetTrp: 0.182 ± 0.054
0.479MetTyr: 0.479 ± 0.103
0.0MetXaa: 0.0 ± 0.0
Asn
3.351AsnAla: 3.351 ± 0.277
0.182AsnCys: 0.182 ± 0.062
1.778AsnAsp: 1.778 ± 0.185
1.505AsnGlu: 1.505 ± 0.2
0.912AsnPhe: 0.912 ± 0.179
3.397AsnGly: 3.397 ± 0.391
0.752AsnHis: 0.752 ± 0.145
1.14AsnIle: 1.14 ± 0.151
0.707AsnLys: 0.707 ± 0.119
2.211AsnLeu: 2.211 ± 0.243
0.593AsnMet: 0.593 ± 0.117
1.094AsnAsn: 1.094 ± 0.183
2.462AsnPro: 2.462 ± 0.301
1.436AsnGln: 1.436 ± 0.181
2.189AsnArg: 2.189 ± 0.237
1.733AsnSer: 1.733 ± 0.189
1.755AsnThr: 1.755 ± 0.184
2.006AsnVal: 2.006 ± 0.219
0.638AsnTrp: 0.638 ± 0.112
1.049AsnTyr: 1.049 ± 0.16
0.0AsnXaa: 0.0 ± 0.0
Pro
4.765ProAla: 4.765 ± 0.365
0.456ProCys: 0.456 ± 0.116
4.103ProAsp: 4.103 ± 0.295
4.035ProGlu: 4.035 ± 0.32
1.14ProPhe: 1.14 ± 0.164
5.471ProGly: 5.471 ± 0.444
1.071ProHis: 1.071 ± 0.161
2.371ProIle: 2.371 ± 0.193
2.303ProLys: 2.303 ± 0.209
3.465ProLeu: 3.465 ± 0.317
1.231ProMet: 1.231 ± 0.167
1.664ProAsn: 1.664 ± 0.214
2.667ProPro: 2.667 ± 0.349
2.28ProGln: 2.28 ± 0.532
2.986ProArg: 2.986 ± 0.264
3.67ProSer: 3.67 ± 0.258
3.739ProThr: 3.739 ± 0.32
4.24ProVal: 4.24 ± 0.35
0.73ProTrp: 0.73 ± 0.128
1.55ProTyr: 1.55 ± 0.193
0.0ProXaa: 0.0 ± 0.0
Gln
4.81GlnAla: 4.81 ± 0.361
0.388GlnCys: 0.388 ± 0.091
1.71GlnAsp: 1.71 ± 0.214
2.12GlnGlu: 2.12 ± 0.217
1.391GlnPhe: 1.391 ± 0.19
2.599GlnGly: 2.599 ± 0.276
0.935GlnHis: 0.935 ± 0.18
2.371GlnIle: 2.371 ± 0.271
1.436GlnLys: 1.436 ± 0.177
3.397GlnLeu: 3.397 ± 0.269
1.596GlnMet: 1.596 ± 0.228
1.436GlnAsn: 1.436 ± 0.176
2.143GlnPro: 2.143 ± 0.461
2.006GlnGln: 2.006 ± 0.5
2.644GlnArg: 2.644 ± 0.217
2.644GlnSer: 2.644 ± 0.269
2.143GlnThr: 2.143 ± 0.203
2.781GlnVal: 2.781 ± 0.269
0.73GlnTrp: 0.73 ± 0.133
1.482GlnTyr: 1.482 ± 0.187
0.0GlnXaa: 0.0 ± 0.0
Arg
6.999ArgAla: 6.999 ± 0.404
0.707ArgCys: 0.707 ± 0.146
4.217ArgAsp: 4.217 ± 0.328
5.357ArgGlu: 5.357 ± 0.405
1.847ArgPhe: 1.847 ± 0.192
4.605ArgGly: 4.605 ± 0.3
1.664ArgHis: 1.664 ± 0.239
3.556ArgIle: 3.556 ± 0.244
2.553ArgLys: 2.553 ± 0.269
5.471ArgLeu: 5.471 ± 0.388
1.755ArgMet: 1.755 ± 0.197
1.938ArgAsn: 1.938 ± 0.227
3.328ArgPro: 3.328 ± 0.328
2.781ArgGln: 2.781 ± 0.273
5.449ArgArg: 5.449 ± 0.443
3.853ArgSer: 3.853 ± 0.277
3.716ArgThr: 3.716 ± 0.292
5.152ArgVal: 5.152 ± 0.395
1.527ArgTrp: 1.527 ± 0.193
2.28ArgTyr: 2.28 ± 0.222
0.0ArgXaa: 0.0 ± 0.0
Ser
6.201SerAla: 6.201 ± 0.378
0.684SerCys: 0.684 ± 0.117
4.537SerAsp: 4.537 ± 0.306
3.511SerGlu: 3.511 ± 0.311
1.983SerPhe: 1.983 ± 0.239
5.859SerGly: 5.859 ± 0.421
0.935SerHis: 0.935 ± 0.162
3.192SerIle: 3.192 ± 0.241
2.234SerLys: 2.234 ± 0.239
5.266SerLeu: 5.266 ± 0.398
1.755SerMet: 1.755 ± 0.243
2.12SerAsn: 2.12 ± 0.21
3.351SerPro: 3.351 ± 0.24
2.394SerGln: 2.394 ± 0.225
3.123SerArg: 3.123 ± 0.261
4.24SerSer: 4.24 ± 0.328
3.214SerThr: 3.214 ± 0.294
4.4SerVal: 4.4 ± 0.355
1.185SerTrp: 1.185 ± 0.154
2.371SerTyr: 2.371 ± 0.207
0.0SerXaa: 0.0 ± 0.0
Thr
5.95ThrAla: 5.95 ± 0.415
0.524ThrCys: 0.524 ± 0.106
4.103ThrAsp: 4.103 ± 0.262
3.739ThrGlu: 3.739 ± 0.311
2.052ThrPhe: 2.052 ± 0.19
5.677ThrGly: 5.677 ± 0.455
1.071ThrHis: 1.071 ± 0.157
3.397ThrIle: 3.397 ± 0.269
2.325ThrLys: 2.325 ± 0.229
5.084ThrLeu: 5.084 ± 0.411
1.117ThrMet: 1.117 ± 0.15
2.211ThrAsn: 2.211 ± 0.262
3.807ThrPro: 3.807 ± 0.294
2.303ThrGln: 2.303 ± 0.226
3.784ThrArg: 3.784 ± 0.289
3.967ThrSer: 3.967 ± 0.421
3.99ThrThr: 3.99 ± 0.374
5.015ThrVal: 5.015 ± 0.392
0.821ThrTrp: 0.821 ± 0.144
2.052ThrTyr: 2.052 ± 0.222
0.0ThrXaa: 0.0 ± 0.0
Val
7.318ValAla: 7.318 ± 0.468
0.57ValCys: 0.57 ± 0.109
4.879ValAsp: 4.879 ± 0.318
4.856ValGlu: 4.856 ± 0.361
1.983ValPhe: 1.983 ± 0.207
5.243ValGly: 5.243 ± 0.424
1.619ValHis: 1.619 ± 0.214
3.283ValIle: 3.283 ± 0.216
2.644ValLys: 2.644 ± 0.251
6.292ValLeu: 6.292 ± 0.397
1.55ValMet: 1.55 ± 0.215
2.28ValAsn: 2.28 ± 0.249
4.309ValPro: 4.309 ± 0.298
3.374ValGln: 3.374 ± 0.274
4.605ValArg: 4.605 ± 0.278
4.605ValSer: 4.605 ± 0.365
5.357ValThr: 5.357 ± 0.414
6.041ValVal: 6.041 ± 0.468
1.436ValTrp: 1.436 ± 0.184
2.029ValTyr: 2.029 ± 0.2
0.0ValXaa: 0.0 ± 0.0
Trp
1.801TrpAla: 1.801 ± 0.19
0.16TrpCys: 0.16 ± 0.066
1.254TrpAsp: 1.254 ± 0.146
1.185TrpGlu: 1.185 ± 0.182
0.73TrpPhe: 0.73 ± 0.143
1.163TrpGly: 1.163 ± 0.161
0.228TrpHis: 0.228 ± 0.068
0.479TrpIle: 0.479 ± 0.115
0.57TrpLys: 0.57 ± 0.12
1.436TrpLeu: 1.436 ± 0.165
0.456TrpMet: 0.456 ± 0.098
0.616TrpAsn: 0.616 ± 0.112
0.935TrpPro: 0.935 ± 0.153
0.752TrpGln: 0.752 ± 0.129
1.14TrpArg: 1.14 ± 0.175
1.071TrpSer: 1.071 ± 0.164
1.231TrpThr: 1.231 ± 0.177
1.185TrpVal: 1.185 ± 0.158
0.251TrpTrp: 0.251 ± 0.079
0.365TrpTyr: 0.365 ± 0.089
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.032TyrAla: 3.032 ± 0.252
0.296TyrCys: 0.296 ± 0.086
2.417TyrAsp: 2.417 ± 0.272
1.733TyrGlu: 1.733 ± 0.184
0.73TyrPhe: 0.73 ± 0.115
2.804TyrGly: 2.804 ± 0.324
0.775TyrHis: 0.775 ± 0.125
1.185TyrIle: 1.185 ± 0.178
0.707TyrLys: 0.707 ± 0.133
2.462TyrLeu: 2.462 ± 0.234
0.616TyrMet: 0.616 ± 0.143
0.866TyrAsn: 0.866 ± 0.152
1.391TyrPro: 1.391 ± 0.217
1.459TyrGln: 1.459 ± 0.159
2.918TyrArg: 2.918 ± 0.275
1.961TyrSer: 1.961 ± 0.259
1.961TyrThr: 1.961 ± 0.224
2.303TyrVal: 2.303 ± 0.271
0.73TyrTrp: 0.73 ± 0.12
0.957TyrTyr: 0.957 ± 0.188
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 220 proteins (43866 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski