Agrobacterium phage Atu_ph08

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; unclassified Podoviridae

Average proteome isoelectric point is 6.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 75 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A223W0E1|A0A223W0E1_9CAUD Uncharacterized protein OS=Agrobacterium phage Atu_ph08 OX=2024265 PE=4 SV=1
MM1 pKa = 6.66THH3 pKa = 6.49EE4 pKa = 4.91DD5 pKa = 3.16LRR7 pKa = 11.84KK8 pKa = 9.48RR9 pKa = 11.84WTEE12 pKa = 3.36ANEE15 pKa = 4.0RR16 pKa = 11.84VEE18 pKa = 5.52LLDD21 pKa = 3.34KK22 pKa = 10.79QRR24 pKa = 11.84YY25 pKa = 6.62QLVEE29 pKa = 3.87HH30 pKa = 6.41TQQEE34 pKa = 4.17WLEE37 pKa = 4.19AQTEE41 pKa = 4.39FQAVVDD47 pKa = 3.85EE48 pKa = 4.85CLNGDD53 pKa = 3.77AFLCEE58 pKa = 4.39ACDD61 pKa = 3.75APIFPGDD68 pKa = 3.45QYY70 pKa = 11.4HH71 pKa = 6.96AGVEE75 pKa = 4.25PRR77 pKa = 11.84CFEE80 pKa = 4.35CAPTYY85 pKa = 10.92QSMIDD90 pKa = 3.68EE91 pKa = 4.76PEE93 pKa = 4.23GFVDD97 pKa = 6.44LVDD100 pKa = 4.77EE101 pKa = 4.93SPSTPEE107 pKa = 3.6NLRR110 pKa = 11.84AAFDD114 pKa = 3.37AHH116 pKa = 6.31IAAGGSPDD124 pKa = 3.57DD125 pKa = 4.96KK126 pKa = 10.73MVEE129 pKa = 4.22VYY131 pKa = 10.5DD132 pKa = 3.8

Molecular weight: 15.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2L0V100|A0A2L0V100_9CAUD Uncharacterized protein OS=Agrobacterium phage Atu_ph08 OX=2024265 PE=4 SV=1
MM1 pKa = 7.16TRR3 pKa = 11.84LPASTYY9 pKa = 10.72RR10 pKa = 11.84KK11 pKa = 7.51NTKK14 pKa = 8.64PKK16 pKa = 10.32KK17 pKa = 8.53RR18 pKa = 11.84VTMARR23 pKa = 11.84RR24 pKa = 11.84NAKK27 pKa = 9.55RR28 pKa = 11.84DD29 pKa = 3.29AAEE32 pKa = 3.99PEE34 pKa = 4.0IVKK37 pKa = 9.17TLEE40 pKa = 3.94AFGMSVFLLHH50 pKa = 6.97EE51 pKa = 4.45PTDD54 pKa = 4.5AIVGYY59 pKa = 10.3RR60 pKa = 11.84GITHH64 pKa = 5.58VVEE67 pKa = 5.62FKK69 pKa = 10.62SGRR72 pKa = 11.84KK73 pKa = 9.72GYY75 pKa = 9.98GAALNDD81 pKa = 3.63NQQAFADD88 pKa = 3.83AWRR91 pKa = 11.84GSAVVTLHH99 pKa = 6.59SVDD102 pKa = 4.1EE103 pKa = 4.36AVVWAQQVRR112 pKa = 11.84RR113 pKa = 11.84GQPP116 pKa = 2.76

Molecular weight: 12.92 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

75

0

75

18864

40

4877

251.5

27.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.23 ± 0.392

0.769 ± 0.183

6.526 ± 0.227

6.648 ± 0.245

3.138 ± 0.17

7.766 ± 0.371

2.062 ± 0.181

4.978 ± 0.246

5.481 ± 0.259

7.581 ± 0.251

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.502 ± 0.124

3.488 ± 0.185

4.962 ± 0.183

3.923 ± 0.218

6.87 ± 0.24

5.211 ± 0.179

5.529 ± 0.127

6.287 ± 0.192

1.771 ± 0.145

2.279 ± 0.112
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski