Gordonia phage DinoDaryn
Average proteome isoelectric point is 6.21
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 82 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1U9WSD3|A0A1U9WSD3_9CAUD Uncharacterized protein OS=Gordonia phage DinoDaryn OX=1958908 GN=77 PE=4 SV=1
MM1 pKa = 7.28 TGEE4 pKa = 4.2 DD5 pKa = 3.64 LGMPEE10 pKa = 4.47 YY11 pKa = 10.75 ASSGYY16 pKa = 9.28 WDD18 pKa = 4.64 PEE20 pKa = 4.2 CSAHH24 pKa = 7.53 GDD26 pKa = 3.58 GEE28 pKa = 4.03 QWQRR32 pKa = 11.84 GKK34 pKa = 11.07 ADD36 pKa = 3.22 GG37 pKa = 3.94
Molecular weight: 4.02 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.833
IPC2_protein 4.113
IPC_protein 3.91
Toseland 3.745
ProMoST 4.088
Dawson 3.91
Bjellqvist 4.075
Wikipedia 3.846
Rodwell 3.757
Grimsley 3.668
Solomon 3.872
Lehninger 3.834
Nozaki 4.05
DTASelect 4.19
Thurlkill 3.821
EMBOSS 3.859
Sillero 4.037
Patrickios 1.926
IPC_peptide 3.872
IPC2_peptide 4.012
IPC2.peptide.svr19 3.935
Protein with the highest isoelectric point:
>tr|A0A1U9WSC2|A0A1U9WSC2_9CAUD NrdH-like glutaredoxin OS=Gordonia phage DinoDaryn OX=1958908 GN=63 PE=4 SV=1
MM1 pKa = 6.42 TAKK4 pKa = 10.53 GAGRR8 pKa = 11.84 STRR11 pKa = 11.84 RR12 pKa = 11.84 WRR14 pKa = 11.84 RR15 pKa = 11.84 LKK17 pKa = 10.92 AEE19 pKa = 4.16 FRR21 pKa = 11.84 RR22 pKa = 11.84 LCADD26 pKa = 3.38 RR27 pKa = 11.84 RR28 pKa = 11.84 APCWICGQPIDD39 pKa = 4.08 YY40 pKa = 10.13 RR41 pKa = 11.84 LEE43 pKa = 4.45 HH44 pKa = 6.38 PHH46 pKa = 6.61 PEE48 pKa = 3.64 AWEE51 pKa = 4.18 PDD53 pKa = 3.45 HH54 pKa = 5.81 YY55 pKa = 11.25 HH56 pKa = 6.6 PVAKK60 pKa = 10.35 RR61 pKa = 11.84 PDD63 pKa = 3.74 LAEE66 pKa = 4.67 DD67 pKa = 3.7 PANLRR72 pKa = 11.84 PSHH75 pKa = 6.16 SSCNRR80 pKa = 11.84 SRR82 pKa = 11.84 GDD84 pKa = 3.41 RR85 pKa = 11.84 EE86 pKa = 4.17 VVLGLGRR93 pKa = 11.84 QSRR96 pKa = 11.84 QWW98 pKa = 3.01
Molecular weight: 11.48 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.336
IPC2_protein 9.253
IPC_protein 9.97
Toseland 10.233
ProMoST 9.999
Dawson 10.394
Bjellqvist 10.175
Wikipedia 10.613
Rodwell 10.452
Grimsley 10.452
Solomon 10.511
Lehninger 10.482
Nozaki 10.35
DTASelect 10.131
Thurlkill 10.277
EMBOSS 10.643
Sillero 10.35
Patrickios 10.218
IPC_peptide 10.511
IPC2_peptide 9.633
IPC2.peptide.svr19 8.298
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
82
0
82
14107
37
1152
172.0
18.72
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.618 ± 0.536
0.801 ± 0.132
6.926 ± 0.257
6.6 ± 0.303
2.935 ± 0.16
9.527 ± 0.458
1.935 ± 0.179
3.36 ± 0.167
3.53 ± 0.224
7.493 ± 0.293
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.297 ± 0.125
2.757 ± 0.173
4.764 ± 0.237
3.027 ± 0.174
7.946 ± 0.361
5.196 ± 0.269
5.607 ± 0.236
8.152 ± 0.271
2.027 ± 0.118
2.502 ± 0.17
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here