Flexivirga caeni
Average proteome isoelectric point is 6.38
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3842 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3M9MCJ1|A0A3M9MCJ1_9MICO TetR/AcrR family transcriptional regulator OS=Flexivirga caeni OX=2294115 GN=EFY87_07605 PE=4 SV=1
MM1 pKa = 7.9 SDD3 pKa = 3.62 LEE5 pKa = 4.32 YY6 pKa = 10.16 PADD9 pKa = 3.54 LRR11 pKa = 11.84 YY12 pKa = 8.63 TSDD15 pKa = 3.62 HH16 pKa = 6.25 EE17 pKa = 4.45 WVKK20 pKa = 11.23 DD21 pKa = 3.5 RR22 pKa = 11.84 GDD24 pKa = 3.41 GVVRR28 pKa = 11.84 VGISAFAQDD37 pKa = 3.46 ALGDD41 pKa = 3.82 VVYY44 pKa = 11.12 VSLPAVGDD52 pKa = 3.97 SVAAGDD58 pKa = 3.5 ACGEE62 pKa = 4.23 VEE64 pKa = 4.37 STKK67 pKa = 10.75 SVSDD71 pKa = 4.02 LYY73 pKa = 11.48 APLAGEE79 pKa = 3.72 ITAINEE85 pKa = 4.06 QLDD88 pKa = 3.89 ATPEE92 pKa = 4.02 LVNNDD97 pKa = 3.67 PYY99 pKa = 11.7 GEE101 pKa = 3.78 GWMYY105 pKa = 10.37 EE106 pKa = 4.32 LKK108 pKa = 10.87 LADD111 pKa = 4.82 DD112 pKa = 4.53 GALAGLQDD120 pKa = 3.34 VDD122 pKa = 4.04 AYY124 pKa = 10.24 KK125 pKa = 10.06 ATLTT129 pKa = 3.95
Molecular weight: 13.68 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.736
IPC2_protein 3.732
IPC_protein 3.719
Toseland 3.503
ProMoST 3.897
Dawson 3.719
Bjellqvist 3.872
Wikipedia 3.668
Rodwell 3.541
Grimsley 3.414
Solomon 3.706
Lehninger 3.656
Nozaki 3.834
DTASelect 4.075
Thurlkill 3.567
EMBOSS 3.681
Sillero 3.846
Patrickios 1.863
IPC_peptide 3.694
IPC2_peptide 3.821
IPC2.peptide.svr19 3.758
Protein with the highest isoelectric point:
>tr|A0A3M9M2S8|A0A3M9M2S8_9MICO SDR family NAD(P)-dependent oxidoreductase OS=Flexivirga caeni OX=2294115 GN=EFY87_15835 PE=3 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.0 RR4 pKa = 11.84 TFQPNNRR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 AKK15 pKa = 8.7 THH17 pKa = 5.15 GFRR20 pKa = 11.84 LRR22 pKa = 11.84 MRR24 pKa = 11.84 TRR26 pKa = 11.84 AGRR29 pKa = 11.84 AILSARR35 pKa = 11.84 RR36 pKa = 11.84 GKK38 pKa = 10.39 GRR40 pKa = 11.84 AKK42 pKa = 10.3 ISAA45 pKa = 3.71
Molecular weight: 5.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.286
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.042
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.676
Grimsley 13.086
Solomon 13.539
Lehninger 13.437
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.398
IPC_peptide 13.539
IPC2_peptide 12.53
IPC2.peptide.svr19 9.215
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3842
0
3842
1225569
28
2003
319.0
34.24
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.032 ± 0.048
0.806 ± 0.011
6.16 ± 0.036
5.116 ± 0.041
2.881 ± 0.024
8.996 ± 0.036
2.363 ± 0.02
4.069 ± 0.023
2.101 ± 0.029
10.098 ± 0.044
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.91 ± 0.016
1.886 ± 0.02
5.536 ± 0.028
3.224 ± 0.022
7.607 ± 0.043
5.595 ± 0.03
6.192 ± 0.027
8.866 ± 0.035
1.567 ± 0.017
1.997 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here