Hypnocyclicus thermotrophus

Taxonomy: cellular organisms; Bacteria; Fusobacteria; Fusobacteriia; Fusobacteriales; Fusobacteriaceae; Hypnocyclicus

Average proteome isoelectric point is 7.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2014 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4R7LA83|A0A4R7LA83_9FUSO Cardiolipin synthase OS=Hypnocyclicus thermotrophus OX=1627895 GN=EV215_0657 PE=3 SV=1
MM1 pKa = 7.5KK2 pKa = 10.22KK3 pKa = 9.99YY4 pKa = 9.92VCVVCGYY11 pKa = 10.88VYY13 pKa = 10.79DD14 pKa = 4.45PEE16 pKa = 6.4IGDD19 pKa = 3.75ADD21 pKa = 3.85AGIEE25 pKa = 4.0PGTSFEE31 pKa = 4.84ALPEE35 pKa = 4.16DD36 pKa = 4.21WVCPVCAVGKK46 pKa = 9.93DD47 pKa = 3.58QFEE50 pKa = 4.32EE51 pKa = 4.19AA52 pKa = 4.45

Molecular weight: 5.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4R7LAA7|A0A4R7LAA7_9FUSO LPP20 lipoprotein OS=Hypnocyclicus thermotrophus OX=1627895 GN=EV215_0621 PE=4 SV=1
MM1 pKa = 7.45RR2 pKa = 11.84HH3 pKa = 4.94NMSYY7 pKa = 10.75RR8 pKa = 11.84KK9 pKa = 9.57LGRR12 pKa = 11.84RR13 pKa = 11.84ADD15 pKa = 3.4HH16 pKa = 6.65RR17 pKa = 11.84KK18 pKa = 10.28AMMMNLTISLLRR30 pKa = 11.84EE31 pKa = 3.7EE32 pKa = 5.4RR33 pKa = 11.84IEE35 pKa = 4.29TTVTRR40 pKa = 11.84AKK42 pKa = 10.12EE43 pKa = 3.68LRR45 pKa = 11.84KK46 pKa = 9.24FAEE49 pKa = 4.06RR50 pKa = 11.84MITLGKK56 pKa = 10.51KK57 pKa = 10.03GDD59 pKa = 3.42LAARR63 pKa = 11.84RR64 pKa = 11.84RR65 pKa = 11.84AFAFLRR71 pKa = 11.84DD72 pKa = 3.76DD73 pKa = 3.76AVVAKK78 pKa = 9.83LFSDD82 pKa = 4.01IAPKK86 pKa = 9.81YY87 pKa = 9.4AEE89 pKa = 4.25RR90 pKa = 11.84NGGYY94 pKa = 7.95TRR96 pKa = 11.84ILKK99 pKa = 9.07TGVRR103 pKa = 11.84RR104 pKa = 11.84GDD106 pKa = 3.58SAEE109 pKa = 3.82LAIIEE114 pKa = 4.33LVKK117 pKa = 11.06

Molecular weight: 13.51 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2014

0

2014

670777

27

1970

333.1

38.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.827 ± 0.06

0.613 ± 0.017

5.204 ± 0.034

8.085 ± 0.064

4.89 ± 0.051

5.619 ± 0.057

1.238 ± 0.019

11.53 ± 0.078

11.076 ± 0.065

9.27 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.122 ± 0.023

7.509 ± 0.071

2.405 ± 0.032

2.017 ± 0.022

2.957 ± 0.035

5.505 ± 0.038

4.633 ± 0.034

5.292 ± 0.048

0.597 ± 0.017

4.611 ± 0.05
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski