Grapevine virus A (isolate Is 151) (GVA)

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Tymovirales; Betaflexiviridae; Trivirinae; Vitivirus; Grapevine virus A

Average proteome isoelectric point is 7.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q67707|CAPSD_GVAIS Capsid protein OS=Grapevine virus A (isolate Is 151) OX=651358 GN=ORF4 PE=4 SV=1
MM1 pKa = 6.99SQEE4 pKa = 3.94GSLGTKK10 pKa = 10.04ASSFEE15 pKa = 3.97PQDD18 pKa = 2.89IKK20 pKa = 11.3VFHH23 pKa = 6.45VKK25 pKa = 10.21RR26 pKa = 11.84STRR29 pKa = 11.84DD30 pKa = 3.19LEE32 pKa = 4.3TLNKK36 pKa = 9.63SLHH39 pKa = 6.32RR40 pKa = 11.84GDD42 pKa = 3.96VYY44 pKa = 10.69NTEE47 pKa = 4.74LIEE50 pKa = 4.25KK51 pKa = 8.5VFPRR55 pKa = 11.84RR56 pKa = 11.84TKK58 pKa = 10.17KK59 pKa = 10.5CVIHH63 pKa = 6.97KK64 pKa = 10.37DD65 pKa = 3.64VIVKK69 pKa = 10.29DD70 pKa = 3.76GRR72 pKa = 11.84VDD74 pKa = 3.69CDD76 pKa = 4.15LDD78 pKa = 4.1IMDD81 pKa = 5.42EE82 pKa = 4.18GLDD85 pKa = 4.39DD86 pKa = 4.0INEE89 pKa = 4.22EE90 pKa = 4.27EE91 pKa = 4.35FPLYY95 pKa = 10.34HH96 pKa = 6.41VGCIVVALMPHH107 pKa = 6.48GKK109 pKa = 9.03NLQGKK114 pKa = 9.05VSVEE118 pKa = 3.99VLDD121 pKa = 3.77TRR123 pKa = 11.84LVDD126 pKa = 3.56GASRR130 pKa = 11.84ISRR133 pKa = 11.84TLMDD137 pKa = 3.24MSKK140 pKa = 10.24PLSACADD147 pKa = 3.56FPGYY151 pKa = 10.31FISTSDD157 pKa = 3.79LLNGYY162 pKa = 6.52TLHH165 pKa = 7.79LSITTTDD172 pKa = 3.39LQFVDD177 pKa = 4.93GVHH180 pKa = 6.45PFSVQLMSIGRR191 pKa = 11.84FCGEE195 pKa = 4.16DD196 pKa = 2.81MKK198 pKa = 10.94TRR200 pKa = 11.84YY201 pKa = 10.26AITEE205 pKa = 4.11TSKK208 pKa = 10.27MLHH211 pKa = 5.24QNILNTEE218 pKa = 4.23GDD220 pKa = 3.75GEE222 pKa = 4.74LIPRR226 pKa = 11.84GVQVQKK232 pKa = 11.16VPDD235 pKa = 3.98TLVMPEE241 pKa = 3.83VFEE244 pKa = 4.5TIKK247 pKa = 11.01KK248 pKa = 10.33FGLKK252 pKa = 9.67TNGTLRR258 pKa = 11.84QEE260 pKa = 4.47GRR262 pKa = 11.84DD263 pKa = 3.22KK264 pKa = 11.44GDD266 pKa = 3.09NRR268 pKa = 11.84RR269 pKa = 11.84VGVGEE274 pKa = 4.19SPTNN278 pKa = 3.44

Molecular weight: 31.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q67708|VSR_GVAIS RNA silencing suppressor OS=Grapevine virus A (isolate Is 151) OX=651358 GN=ORF5 PE=3 SV=2
MM1 pKa = 8.42DD2 pKa = 5.95DD3 pKa = 3.85PSFLTGRR10 pKa = 11.84STFAKK15 pKa = 9.46RR16 pKa = 11.84RR17 pKa = 11.84RR18 pKa = 11.84ARR20 pKa = 11.84RR21 pKa = 11.84MNVCKK26 pKa = 10.46CGAIMHH32 pKa = 6.32NNKK35 pKa = 9.59DD36 pKa = 4.19CKK38 pKa = 10.37SSSISSHH45 pKa = 5.93KK46 pKa = 10.17LDD48 pKa = 3.11RR49 pKa = 11.84LRR51 pKa = 11.84FVKK54 pKa = 10.31EE55 pKa = 3.45GRR57 pKa = 11.84VALTGEE63 pKa = 4.29TPVYY67 pKa = 8.51RR68 pKa = 11.84TWVKK72 pKa = 9.15WVEE75 pKa = 4.28TEE77 pKa = 3.6YY78 pKa = 10.74HH79 pKa = 6.0IYY81 pKa = 10.08IVEE84 pKa = 4.14TSDD87 pKa = 5.92DD88 pKa = 3.61EE89 pKa = 4.72DD90 pKa = 3.59

Molecular weight: 10.53 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

2450

90

1707

490.0

55.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.551 ± 1.047

1.837 ± 0.232

6.286 ± 0.554

8.122 ± 1.179

3.796 ± 0.083

6.939 ± 1.115

2.898 ± 0.37

5.347 ± 0.141

6.939 ± 0.573

9.755 ± 0.94

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.776 ± 0.458

3.306 ± 0.317

3.102 ± 0.36

2.694 ± 0.36

6.49 ± 0.519

7.592 ± 1.12

5.347 ± 0.684

6.531 ± 0.842

0.776 ± 0.319

3.918 ± 0.522
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski