Candidatus Mancarchaeum acidiphilum

Taxonomy: cellular organisms; Archaea; DPANN group; Candidatus Micrarchaeota; Candidatus Mancarchaeum

Average proteome isoelectric point is 6.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 944 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A218NM28|A0A218NM28_9ARCH AAA+ superfamily ATPase OS=Candidatus Mancarchaeum acidiphilum OX=1920749 GN=Mia14_0183 PE=4 SV=1
MM1 pKa = 7.19TNKK4 pKa = 10.11KK5 pKa = 10.55GFIFTLDD12 pKa = 3.16AVFALIVAAAAISILLYY29 pKa = 9.95IHH31 pKa = 6.91FIAPISYY38 pKa = 9.65QSSISQVEE46 pKa = 4.28DD47 pKa = 3.55DD48 pKa = 4.63LQVLTSTPLGGYY60 pKa = 10.27LSALDD65 pKa = 4.41NPFSGYY71 pKa = 7.57TRR73 pKa = 11.84SYY75 pKa = 8.74NTFGAASFDD84 pKa = 3.99GYY86 pKa = 11.45NSYY89 pKa = 10.57ISSQLSVPAAEE100 pKa = 4.14NLTYY104 pKa = 10.66VAWIYY109 pKa = 11.19LNYY112 pKa = 10.17IPDD115 pKa = 3.74NNAGIISAVSSYY127 pKa = 11.38NYY129 pKa = 10.31GSMSIFLNGNCNIQVLVTNNTGNTFADD156 pKa = 4.04DD157 pKa = 3.79SPYY160 pKa = 10.48CLKK163 pKa = 10.19PHH165 pKa = 6.85RR166 pKa = 11.84WYY168 pKa = 10.82QVVATEE174 pKa = 4.66NEE176 pKa = 4.75SISQDD181 pKa = 2.88GKK183 pKa = 11.25VIGGNYY189 pKa = 7.91TLYY192 pKa = 10.57INGEE196 pKa = 3.92WNLTQKK202 pKa = 10.62FSGALEE208 pKa = 4.27GATGFNIGLFSYY220 pKa = 10.16QGAYY224 pKa = 9.48FPGYY228 pKa = 9.12ISNVQIYY235 pKa = 9.66GSRR238 pKa = 11.84LTRR241 pKa = 11.84TQVSSLYY248 pKa = 10.72DD249 pKa = 3.82EE250 pKa = 4.61GTMGIPLRR258 pKa = 11.84SSNLLEE264 pKa = 4.18WYY266 pKa = 8.98PLNSEE271 pKa = 4.33SNPNVFKK278 pKa = 10.74SYY280 pKa = 10.87NVTYY284 pKa = 10.47FNTGDD289 pKa = 3.24ISIGNVNLTEE299 pKa = 4.12NQSMLSAIADD309 pKa = 4.29LYY311 pKa = 11.66LNDD314 pKa = 4.07QAPEE318 pKa = 3.6ADD320 pKa = 3.36IIMDD324 pKa = 3.88NLYY327 pKa = 10.59HH328 pKa = 7.18NEE330 pKa = 3.83DD331 pKa = 3.02TGIYY335 pKa = 9.75INSTYY340 pKa = 11.17APDD343 pKa = 3.79LKK345 pKa = 10.84VAYY348 pKa = 9.97FDD350 pKa = 3.78GTAGFYY356 pKa = 10.83APNFTYY362 pKa = 10.11PGKK365 pKa = 10.43NYY367 pKa = 9.71TINQWFYY374 pKa = 11.36LSQADD379 pKa = 4.51DD380 pKa = 3.67PTSGVGGGQSPIVDD394 pKa = 4.21IYY396 pKa = 11.24NGSANGGIGGSQNLDD411 pKa = 3.71FGGTWAGGSSNNFGIGEE428 pKa = 4.29DD429 pKa = 3.82WPSDD433 pKa = 3.26WEE435 pKa = 4.44FCSTNAGTVFPNKK448 pKa = 8.67WYY450 pKa = 9.31NAVVSVVNYY459 pKa = 9.52TNVTIYY465 pKa = 11.12LNGVEE470 pKa = 5.35AKK472 pKa = 10.3NCLLTVPISGMEE484 pKa = 3.99YY485 pKa = 7.32PTLGIGDD492 pKa = 4.18NPPGGNEE499 pKa = 3.9LATAAISNVQVYY511 pKa = 10.55DD512 pKa = 3.52SAVNANQAVTLYY524 pKa = 10.82DD525 pKa = 3.35EE526 pKa = 5.92GIAGEE531 pKa = 4.38PVSSSVIGWYY541 pKa = 9.49PLLGNFNDD549 pKa = 4.11YY550 pKa = 11.21SGIGHH555 pKa = 6.5SGFGGNGGSLNPDD568 pKa = 2.53NVRR571 pKa = 11.84FIEE574 pKa = 4.89SNFTPPGFASSDD586 pKa = 3.67IISQASSPEE595 pKa = 3.86LLNVNGTMNVYY606 pKa = 9.9NVSVLSWGG614 pKa = 3.47

Molecular weight: 66.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A218NNS2|A0A218NNS2_9ARCH Chromosome segregation ATPase OS=Candidatus Mancarchaeum acidiphilum OX=1920749 GN=Mia14_0839 PE=4 SV=1
MM1 pKa = 7.57GSIPRR6 pKa = 11.84KK7 pKa = 7.15WKK9 pKa = 10.06KK10 pKa = 9.08KK11 pKa = 10.29GRR13 pKa = 11.84MRR15 pKa = 11.84WKK17 pKa = 8.72WKK19 pKa = 8.65KK20 pKa = 10.1KK21 pKa = 7.96RR22 pKa = 11.84RR23 pKa = 11.84KK24 pKa = 9.05RR25 pKa = 11.84LKK27 pKa = 8.75RR28 pKa = 11.84AQKK31 pKa = 10.23RR32 pKa = 11.84RR33 pKa = 11.84VGEE36 pKa = 3.92LL37 pKa = 3.03

Molecular weight: 4.78 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

944

0

944

277313

35

2805

293.8

32.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.234 ± 0.076

0.675 ± 0.028

5.387 ± 0.076

6.324 ± 0.095

4.351 ± 0.067

6.562 ± 0.082

1.378 ± 0.028

8.965 ± 0.091

8.491 ± 0.111

9.002 ± 0.1

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.608 ± 0.037

5.89 ± 0.108

3.453 ± 0.051

2.154 ± 0.034

3.708 ± 0.075

8.457 ± 0.11

4.784 ± 0.105

6.095 ± 0.064

0.74 ± 0.025

4.739 ± 0.068
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski