Chenuda virus
Average proteome isoelectric point is 7.5
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 12 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0H4MKD1|A0A0H4MKD1_9REOV Core protein VP7 OS=Chenuda virus OX=40065 GN=VP7 PE=3 SV=1
MM1 pKa = 7.89 DD2 pKa = 5.84 AYY4 pKa = 10.62 NARR7 pKa = 11.84 ALSVLEE13 pKa = 4.05 GLALAADD20 pKa = 4.33 PRR22 pKa = 11.84 AHH24 pKa = 7.34 RR25 pKa = 11.84 DD26 pKa = 3.47 PVSEE30 pKa = 4.18 TTLSIFMMRR39 pKa = 11.84 FNSTTTRR46 pKa = 11.84 PIVGAPTTRR55 pKa = 11.84 EE56 pKa = 3.42 ARR58 pKa = 11.84 RR59 pKa = 11.84 NNFYY63 pKa = 10.95 AALDD67 pKa = 3.52 VAYY70 pKa = 9.41 AALGITSQFLMPGYY84 pKa = 9.49 VQNPQTLAILARR96 pKa = 11.84 DD97 pKa = 4.53 EE98 pKa = 4.44 IPYY101 pKa = 9.29 TPSTFRR107 pKa = 11.84 RR108 pKa = 11.84 VQRR111 pKa = 11.84 IRR113 pKa = 11.84 ICSEE117 pKa = 3.82 GACTFRR123 pKa = 11.84 EE124 pKa = 4.54 EE125 pKa = 4.06 YY126 pKa = 10.43 FPYY129 pKa = 10.43 QNYY132 pKa = 8.5 TMLTGRR138 pKa = 11.84 AMPINPPADD147 pKa = 3.9 GGPRR151 pKa = 11.84 CYY153 pKa = 10.45 LVDD156 pKa = 4.16 PNTVQVHH163 pKa = 5.21 VEE165 pKa = 4.04 PEE167 pKa = 3.59 QTIAVTDD174 pKa = 3.86 VVVPTAPNWVAAEE187 pKa = 4.07 LAWYY191 pKa = 8.89 IQTNGPGANAAEE203 pKa = 5.69 GYY205 pKa = 10.1 AQDD208 pKa = 2.92 VDD210 pKa = 4.72 VYY212 pKa = 11.37 VNDD215 pKa = 3.47 RR216 pKa = 11.84 RR217 pKa = 11.84 LPAGLPYY224 pKa = 10.58 RR225 pKa = 11.84 LNPGDD230 pKa = 4.0 RR231 pKa = 11.84 VEE233 pKa = 4.62 LTNHH237 pKa = 7.21 DD238 pKa = 4.35 DD239 pKa = 4.1 FNLGTAMFFIRR250 pKa = 11.84 RR251 pKa = 11.84 YY252 pKa = 8.65 WSAAPPQVIYY262 pKa = 11.04 DD263 pKa = 3.72 SMEE266 pKa = 4.65 ADD268 pKa = 2.79 ICAVYY273 pKa = 9.93 IYY275 pKa = 10.46 HH276 pKa = 7.49 DD277 pKa = 4.18 RR278 pKa = 11.84 TWHH281 pKa = 6.4 RR282 pKa = 11.84 LRR284 pKa = 11.84 SYY286 pKa = 10.44 ICTQVGLPPTYY297 pKa = 10.08 YY298 pKa = 9.9 PSEE301 pKa = 4.07 ATQEE305 pKa = 3.94 PRR307 pKa = 11.84 RR308 pKa = 11.84 VLTIAILSRR317 pKa = 11.84 LFDD320 pKa = 3.73 VYY322 pKa = 10.84 CALSPEE328 pKa = 3.97 IHH330 pKa = 6.56 LPAPEE335 pKa = 4.14 VAPGDD340 pKa = 3.39 LAQRR344 pKa = 11.84 LRR346 pKa = 11.84 EE347 pKa = 4.04 ALQTLRR353 pKa = 11.84 GAPPPAAPRR362 pKa = 11.84 GGQQ365 pKa = 2.99
Molecular weight: 40.71 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.381
IPC2_protein 5.537
IPC_protein 5.525
Toseland 5.766
ProMoST 5.792
Dawson 5.69
Bjellqvist 5.741
Wikipedia 5.664
Rodwell 5.652
Grimsley 5.842
Solomon 5.69
Lehninger 5.677
Nozaki 5.906
DTASelect 6.097
Thurlkill 6.008
EMBOSS 5.97
Sillero 6.008
Patrickios 3.478
IPC_peptide 5.703
IPC2_peptide 6.02
IPC2.peptide.svr19 5.974
Protein with the highest isoelectric point:
>tr|A0A0H4M927|A0A0H4M927_9REOV Outer capsid protein OS=Chenuda virus OX=40065 GN=VP4 PE=4 SV=1
MM1 pKa = 7.53 LSVADD6 pKa = 3.96 AKK8 pKa = 10.7 RR9 pKa = 11.84 RR10 pKa = 11.84 EE11 pKa = 4.16 QMAGTEE17 pKa = 4.07 MRR19 pKa = 11.84 PLVPSAPRR27 pKa = 11.84 PLPGAMPEE35 pKa = 3.94 MALGVLQNALTSSTGANEE53 pKa = 3.75 TAKK56 pKa = 10.74 NEE58 pKa = 3.88 KK59 pKa = 9.6 AAYY62 pKa = 8.52 GAASEE67 pKa = 4.67 VMRR70 pKa = 11.84 DD71 pKa = 3.74 DD72 pKa = 3.95 PATRR76 pKa = 11.84 QLKK79 pKa = 8.69 VHH81 pKa = 6.07 VSVTAIAEE89 pKa = 3.95 LEE91 pKa = 3.93 EE92 pKa = 4.28 RR93 pKa = 11.84 YY94 pKa = 10.29 KK95 pKa = 10.36 RR96 pKa = 11.84 VRR98 pKa = 11.84 RR99 pKa = 11.84 KK100 pKa = 9.97 KK101 pKa = 10.23 CACKK105 pKa = 9.17 WLQAISSALVLTTSIAMSIVTASTWVEE132 pKa = 3.58 NTLVKK137 pKa = 10.81 NGVPVYY143 pKa = 9.47 MIISGLTTVAMVAGRR158 pKa = 11.84 MHH160 pKa = 6.59 AHH162 pKa = 6.58 ARR164 pKa = 11.84 TATRR168 pKa = 11.84 TIKK171 pKa = 10.37 RR172 pKa = 11.84 DD173 pKa = 3.41 LVKK176 pKa = 10.6 KK177 pKa = 10.53 KK178 pKa = 10.71 SYY180 pKa = 10.02 VQLAVAMGTPLPGTVAAPAKK200 pKa = 10.36 AGTSVGAASIVDD212 pKa = 3.75 DD213 pKa = 4.33 LVARR217 pKa = 11.84 GWQPLPP223 pKa = 3.77
Molecular weight: 23.69 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.18
IPC2_protein 9.385
IPC_protein 9.472
Toseland 10.365
ProMoST 9.911
Dawson 10.467
Bjellqvist 10.087
Wikipedia 10.599
Rodwell 10.965
Grimsley 10.511
Solomon 10.511
Lehninger 10.496
Nozaki 10.35
DTASelect 10.087
Thurlkill 10.35
EMBOSS 10.745
Sillero 10.394
Patrickios 10.687
IPC_peptide 10.511
IPC2_peptide 8.697
IPC2.peptide.svr19 8.496
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
12
0
12
6145
183
1285
512.1
57.25
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.301 ± 0.604
1.383 ± 0.242
5.435 ± 0.341
5.891 ± 0.249
3.466 ± 0.333
5.696 ± 0.395
2.571 ± 0.226
5.028 ± 0.275
2.799 ± 0.503
9.552 ± 0.362
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.457 ± 0.264
2.587 ± 0.212
6.151 ± 0.427
3.531 ± 0.417
9.39 ± 0.431
5.663 ± 0.391
6.363 ± 0.37
7.681 ± 0.324
1.155 ± 0.187
2.897 ± 0.276
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here