White bream virus (isolate Blicca bjoerkna L./Germany/DF24/00) (WBV)
Average proteome isoelectric point is 7.15
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|Q008X6|R1AB_WBV24 Replicase polyprotein 1ab OS=White bream virus (isolate Blicca bjoerkna L./Germany/DF24/00) OX=766180 GN=rep PE=1 SV=1
MM1 pKa = 7.65 SILFGNRR8 pKa = 11.84 QANATKK14 pKa = 10.05 RR15 pKa = 11.84 SDD17 pKa = 3.37 MASVARR23 pKa = 11.84 AVYY26 pKa = 9.68 EE27 pKa = 3.79 VDD29 pKa = 5.43 LISTKK34 pKa = 8.04 YY35 pKa = 10.51 ARR37 pKa = 11.84 RR38 pKa = 11.84 TQEE41 pKa = 3.55 RR42 pKa = 11.84 LAHH45 pKa = 6.08 NKK47 pKa = 8.43 HH48 pKa = 6.27 AKK50 pKa = 9.28 PSYY53 pKa = 8.48 PSVFFGRR60 pKa = 11.84 RR61 pKa = 11.84 MKK63 pKa = 10.46 AVKK66 pKa = 9.99 EE67 pKa = 4.08 PTFTPSTLFFEE78 pKa = 5.05 EE79 pKa = 3.88 ATLPKK84 pKa = 10.48 VLASKK89 pKa = 10.5 AKK91 pKa = 9.22 PDD93 pKa = 3.49 TGIKK97 pKa = 7.66 TRR99 pKa = 11.84 RR100 pKa = 11.84 VYY102 pKa = 10.78 VADD105 pKa = 3.44 SLTINGHH112 pKa = 5.36 TYY114 pKa = 10.1 PIVGHH119 pKa = 5.37 FVEE122 pKa = 4.63 MAVSKK127 pKa = 11.1 KK128 pKa = 9.45 EE129 pKa = 3.9 AFPIQPKK136 pKa = 9.6 RR137 pKa = 11.84 VKK139 pKa = 10.09 PKK141 pKa = 10.13 PLMAKK146 pKa = 9.14 PIPNIRR152 pKa = 11.84 RR153 pKa = 11.84 TFLTPEE159 pKa = 3.92 EE160 pKa = 4.27 RR161 pKa = 11.84 TNTPTTPTTTTTTPFVAGEE180 pKa = 4.18 TAGPTIEE187 pKa = 4.19 YY188 pKa = 8.65 TPTSIDD194 pKa = 4.03 LPFAMPTVKK203 pKa = 10.22 QIKK206 pKa = 9.37 EE207 pKa = 3.88 NAHH210 pKa = 5.78 TILRR214 pKa = 11.84 EE215 pKa = 3.7 QDD217 pKa = 2.99 DD218 pKa = 4.56 CLRR221 pKa = 11.84 FAQTALFKK229 pKa = 10.97 HH230 pKa = 6.49 LGTVTHH236 pKa = 6.34 TTPNHH241 pKa = 5.27 ATTFQVKK248 pKa = 9.78 GRR250 pKa = 11.84 TSLLTFEE257 pKa = 4.44 WRR259 pKa = 11.84 KK260 pKa = 6.05 TTQSPLTDD268 pKa = 2.89 GHH270 pKa = 6.86 FYY272 pKa = 10.88 LQTANNHH279 pKa = 5.94 AEE281 pKa = 3.88 LMQPVEE287 pKa = 4.49 GKK289 pKa = 8.56 LTTIFTTTIQQGTTHH304 pKa = 6.79 SLHH307 pKa = 7.08 LIKK310 pKa = 10.49 QEE312 pKa = 4.01 SARR315 pKa = 11.84 TLKK318 pKa = 9.14 TRR320 pKa = 11.84 KK321 pKa = 8.82 PLKK324 pKa = 10.25 LVTYY328 pKa = 9.52 KK329 pKa = 10.56 QEE331 pKa = 4.28 TPTTTITPQSLKK343 pKa = 9.77 KK344 pKa = 9.12 TITYY348 pKa = 9.97 IPGSFCINVAEE359 pKa = 4.26 PTLQSVMRR367 pKa = 11.84 RR368 pKa = 11.84 QPLTPTPNDD377 pKa = 3.33 ALLQIYY383 pKa = 9.59 HH384 pKa = 7.35 KK385 pKa = 10.51 LGCTTKK391 pKa = 10.42 SPNHH395 pKa = 6.48 ASTFEE400 pKa = 4.24 LFGNTYY406 pKa = 7.14 TWYY409 pKa = 9.83 PVQHH413 pKa = 6.42 TNNLLHH419 pKa = 7.12 KK420 pKa = 9.98 DD421 pKa = 3.29 PNRR424 pKa = 11.84 RR425 pKa = 11.84 FFLHH429 pKa = 6.06 ITGQTPQLLIRR440 pKa = 11.84 TEE442 pKa = 3.89 RR443 pKa = 11.84 KK444 pKa = 7.96 TFLTLQDD451 pKa = 3.49 EE452 pKa = 4.7 VTYY455 pKa = 10.67 ISGKK459 pKa = 9.63 LFVMNHH465 pKa = 5.57 APIQGEE471 pKa = 4.6 YY472 pKa = 8.72 KK473 pKa = 9.09 TQTAEE478 pKa = 3.81 WVGSYY483 pKa = 11.2 NMAKK487 pKa = 8.73 TPKK490 pKa = 9.72 AIKK493 pKa = 8.84 PAKK496 pKa = 7.56 TVEE499 pKa = 4.36 YY500 pKa = 10.38 INTTPCHH507 pKa = 6.49 KK508 pKa = 9.48 PATMPAPITYY518 pKa = 9.27 RR519 pKa = 11.84 QCPYY523 pKa = 9.25 TWTLHH528 pKa = 5.77 EE529 pKa = 4.26 PSISKK534 pKa = 8.33 VQRR537 pKa = 11.84 NLFHH541 pKa = 6.9 IPKK544 pKa = 9.08 TATNCLDD551 pKa = 4.99 RR552 pKa = 11.84 IQKK555 pKa = 10.51 ALFPEE560 pKa = 4.63 IVTSNQHH567 pKa = 5.32 FPIGFTIQTDD577 pKa = 3.53 TTMQSYY583 pKa = 9.42 EE584 pKa = 3.7 WAILCKK590 pKa = 10.11 KK591 pKa = 7.29 VTRR594 pKa = 11.84 TYY596 pKa = 10.54 YY597 pKa = 10.71 LAVQNHH603 pKa = 6.52 HH604 pKa = 5.71 ATLWYY609 pKa = 10.23 KK610 pKa = 10.22 CAQVYY615 pKa = 8.02 MCLSDD620 pKa = 5.94 DD621 pKa = 3.65 IAPTTEE627 pKa = 4.11 LQGSVYY633 pKa = 10.28 ILNKK637 pKa = 8.57 VTDD640 pKa = 3.84 PGFYY644 pKa = 10.03 QNTRR648 pKa = 11.84 QWCGSNDD655 pKa = 3.54 DD656 pKa = 3.72 LHH658 pKa = 6.65 EE659 pKa = 4.7 PKK661 pKa = 10.58 HH662 pKa = 6.05 LIKK665 pKa = 10.87 NLANGDD671 pKa = 3.87 VNNIAHH677 pKa = 7.03 CSLTPWTTTPLVYY690 pKa = 9.79 STQKK694 pKa = 10.92 NKK696 pKa = 8.65 LTRR699 pKa = 11.84 KK700 pKa = 9.81 LIHH703 pKa = 6.64 YY704 pKa = 8.81 YY705 pKa = 9.9 NATYY709 pKa = 9.1 TVQVPKK715 pKa = 10.17 EE716 pKa = 4.12 NPNAQPSTMKK726 pKa = 10.2 CAYY729 pKa = 9.27 KK730 pKa = 10.49 AYY732 pKa = 9.6 IDD734 pKa = 4.11 LATPTEE740 pKa = 4.19 LQIHH744 pKa = 7.19 LDD746 pKa = 3.65 LEE748 pKa = 4.68 IQGKK752 pKa = 9.99 LYY754 pKa = 10.85 SQTAQKK760 pKa = 10.45 KK761 pKa = 8.4 GSKK764 pKa = 9.5 FNKK767 pKa = 9.79 LDD769 pKa = 3.15 IPTFGDD775 pKa = 3.21 ILKK778 pKa = 8.72 GTLYY782 pKa = 10.71 VFSSKK787 pKa = 10.76 VLLYY791 pKa = 7.73 EE792 pKa = 3.8 QPKK795 pKa = 10.04 RR796 pKa = 11.84 CTSVCFHH803 pKa = 7.04 LPNNAQSFFFNTEE816 pKa = 4.34 TIQTFEE822 pKa = 4.66 DD823 pKa = 3.61 LFARR827 pKa = 11.84 ISSEE831 pKa = 3.92 EE832 pKa = 4.03 VEE834 pKa = 4.75 DD835 pKa = 3.9 NLVLIKK841 pKa = 10.97 GFVPCLGAIYY851 pKa = 8.69 ITKK854 pKa = 9.81 DD855 pKa = 3.08 LKK857 pKa = 10.92 FIQPEE862 pKa = 3.93 LKK864 pKa = 10.23 EE865 pKa = 4.36 DD866 pKa = 4.1 FKK868 pKa = 11.64 HH869 pKa = 5.08 PTTYY873 pKa = 9.58 YY874 pKa = 10.5 TFTTTVDD881 pKa = 3.71 PEE883 pKa = 4.01 QVLYY887 pKa = 10.74 SLHH890 pKa = 7.01 PSFTDD895 pKa = 3.12 IVPPHH900 pKa = 6.77 GFPYY904 pKa = 9.75 YY905 pKa = 10.55 TFAKK909 pKa = 10.0 LNNHH913 pKa = 5.44 IDD915 pKa = 3.07 AWNITDD921 pKa = 4.42 DD922 pKa = 4.34 QADD925 pKa = 4.1 TLAEE929 pKa = 4.09 AQPIIFQWPTEE940 pKa = 4.11 EE941 pKa = 4.08 ATITTPYY948 pKa = 10.29 KK949 pKa = 9.26 VLHH952 pKa = 5.82 YY953 pKa = 10.06 EE954 pKa = 3.91 HH955 pKa = 7.39 LEE957 pKa = 3.97 GLDD960 pKa = 3.95 YY961 pKa = 11.06 ISLSSFNTVEE971 pKa = 4.49 CPEE974 pKa = 4.27 EE975 pKa = 4.04 TQSAHH980 pKa = 6.76 DD981 pKa = 4.01 SSSEE985 pKa = 4.05 SEE987 pKa = 4.5 SEE989 pKa = 4.39 DD990 pKa = 3.48 EE991 pKa = 4.63 EE992 pKa = 5.54 LPAHH996 pKa = 6.65 PLSNAPSQASLSSVASTAPPTSPTSSPTPSPTPLQQQVGLKK1037 pKa = 9.9 GKK1039 pKa = 8.3 EE1040 pKa = 3.94 VPVGGWVLVSEE1051 pKa = 4.79 EE1052 pKa = 4.25 EE1053 pKa = 4.53 TPSEE1057 pKa = 4.11 EE1058 pKa = 3.89 VDD1060 pKa = 4.22 SPKK1063 pKa = 10.62 LLPNEE1068 pKa = 4.26 VPLSFDD1074 pKa = 3.08 FDD1076 pKa = 4.39 LPIEE1080 pKa = 4.73 PITRR1084 pKa = 11.84 PISPEE1089 pKa = 3.69 LQQPILTHH1097 pKa = 5.74 YY1098 pKa = 7.33 EE1099 pKa = 4.26 HH1100 pKa = 7.14 PTSPTPSVEE1109 pKa = 3.66 IEE1111 pKa = 3.83 IDD1113 pKa = 3.35 FGSYY1117 pKa = 10.81 EE1118 pKa = 4.24 NLTLQTEE1125 pKa = 4.51 EE1126 pKa = 4.34 TVTTEE1131 pKa = 4.14 VQPEE1135 pKa = 4.2 PTPAPTPEE1143 pKa = 3.9 PTIVTEE1149 pKa = 4.63 TVQEE1153 pKa = 4.42 TPVPTEE1159 pKa = 4.01 TTQEE1163 pKa = 4.12 STPEE1167 pKa = 3.98 STPEE1171 pKa = 3.9 STPEE1175 pKa = 3.74 PTPEE1179 pKa = 4.03 STSEE1183 pKa = 3.94 STLEE1187 pKa = 4.16 PEE1189 pKa = 4.78 HH1190 pKa = 6.49 VATPSQSPTHH1200 pKa = 5.51 ITVTEE1205 pKa = 4.11 ITHH1208 pKa = 6.06 EE1209 pKa = 4.32 PEE1211 pKa = 4.61 TPDD1214 pKa = 3.06 SWSEE1218 pKa = 4.24 RR1219 pKa = 11.84 YY1220 pKa = 10.08 DD1221 pKa = 3.4 STSNIPEE1228 pKa = 4.11 VFNQLSFGSTDD1239 pKa = 3.13 SVKK1242 pKa = 9.32 ITTPKK1247 pKa = 10.27 TEE1249 pKa = 4.45 TPDD1252 pKa = 3.69 EE1253 pKa = 4.42 PQQPTVEE1260 pKa = 4.42 TVSAAQQLLQIVQTATPDD1278 pKa = 3.06 IAQLMSEE1285 pKa = 4.79 LPPYY1289 pKa = 10.44 RR1290 pKa = 11.84 LICIGSYY1297 pKa = 10.77 CPILAEE1303 pKa = 4.74 NISKK1307 pKa = 9.92 QLPTAVTTPTDD1318 pKa = 3.35 ADD1320 pKa = 3.45 IPTVIFNVSEE1330 pKa = 4.08 EE1331 pKa = 4.35 TMDD1334 pKa = 3.64 TVINTVKK1341 pKa = 10.0 TKK1343 pKa = 10.36 HH1344 pKa = 5.21 QANHH1348 pKa = 5.54 LTFSLTTIIALDD1360 pKa = 3.75 VPKK1363 pKa = 10.75 DD1364 pKa = 3.42 KK1365 pKa = 11.0 SLPLQQIYY1373 pKa = 10.99 DD1374 pKa = 3.71 KK1375 pKa = 10.64 LTQQDD1380 pKa = 4.39 YY1381 pKa = 8.3 NTDD1384 pKa = 4.42 FIYY1387 pKa = 10.41 EE1388 pKa = 4.13 SHH1390 pKa = 6.45 HH1391 pKa = 5.68 RR1392 pKa = 11.84 QPKK1395 pKa = 9.34 EE1396 pKa = 4.01 SLTHH1400 pKa = 6.3 ASVLSAYY1407 pKa = 6.85 TASYY1411 pKa = 7.98 KK1412 pKa = 8.13 TTAIKK1417 pKa = 10.45 SIADD1421 pKa = 3.47 NAVVLDD1427 pKa = 3.53 IGYY1430 pKa = 10.6 GKK1432 pKa = 11.04 GNDD1435 pKa = 3.68 GPRR1438 pKa = 11.84 YY1439 pKa = 9.05 AVRR1442 pKa = 11.84 PLTVTGIDD1450 pKa = 3.05 TAARR1454 pKa = 11.84 MLAIADD1460 pKa = 3.56 QNKK1463 pKa = 9.11 PEE1465 pKa = 4.13 NVTLVKK1471 pKa = 10.56 QGFFTHH1477 pKa = 5.83 ITKK1480 pKa = 9.96 TSNTYY1485 pKa = 7.07 THH1487 pKa = 6.9 VIAFNSLHH1495 pKa = 6.1 YY1496 pKa = 9.82 PLASSHH1502 pKa = 7.45 PDD1504 pKa = 3.15 TLVQRR1509 pKa = 11.84 LPTCPANILIPCHH1522 pKa = 6.35 HH1523 pKa = 7.2 LLEE1526 pKa = 5.62 GIQTPTYY1533 pKa = 10.66 SVVKK1537 pKa = 10.45 DD1538 pKa = 3.19 EE1539 pKa = 6.15 DD1540 pKa = 3.57 MWCVKK1545 pKa = 7.28 VTKK1548 pKa = 10.67 NEE1550 pKa = 4.46 FIEE1553 pKa = 4.26 SSYY1556 pKa = 11.53 NYY1558 pKa = 10.57 DD1559 pKa = 3.47 VFVKK1563 pKa = 10.54 ALEE1566 pKa = 4.34 SKK1568 pKa = 9.1 YY1569 pKa = 10.47 HH1570 pKa = 4.28 VTIGSLLDD1578 pKa = 3.84 CVEE1581 pKa = 4.4 KK1582 pKa = 10.61 PSTRR1586 pKa = 11.84 SITPTLWTAMRR1597 pKa = 11.84 NFVNNDD1603 pKa = 2.64 QEE1605 pKa = 4.32 MQRR1608 pKa = 11.84 ILSGYY1613 pKa = 7.82 ITFNLTPLPPKK1624 pKa = 10.67 VEE1626 pKa = 5.09 IINDD1630 pKa = 3.58 WLDD1633 pKa = 3.33 NNATVTINNPFASNEE1648 pKa = 3.87 GVTFAVHH1655 pKa = 6.71 NIGAITTTEE1664 pKa = 3.97 GEE1666 pKa = 4.64 FIVNAANKK1674 pKa = 9.24 QLNNGTGVTGAIFAAHH1690 pKa = 6.91 DD1691 pKa = 4.07 KK1692 pKa = 10.31 EE1693 pKa = 5.12 LKK1695 pKa = 8.9 LTQAIKK1701 pKa = 10.74 ALPTYY1706 pKa = 9.85 GASDD1710 pKa = 3.36 KK1711 pKa = 11.33 LEE1713 pKa = 4.1 SHH1715 pKa = 5.22 QHH1717 pKa = 4.1 VVQTIIKK1724 pKa = 9.34 NNNSTHH1730 pKa = 7.32 AINILHH1736 pKa = 7.0 AAAPIKK1742 pKa = 10.11 VKK1744 pKa = 9.72 CTSKK1748 pKa = 10.87 NPEE1751 pKa = 3.56 VLLAHH1756 pKa = 6.69 NEE1758 pKa = 4.16 TAQSEE1763 pKa = 4.37 LKK1765 pKa = 8.77 EE1766 pKa = 4.25 TYY1768 pKa = 9.89 KK1769 pKa = 11.08 AIVDD1773 pKa = 3.61 YY1774 pKa = 11.21 AQLNKK1779 pKa = 9.12 LTHH1782 pKa = 6.54 IYY1784 pKa = 10.61 LPLFGAGAYY1793 pKa = 7.72 GHH1795 pKa = 6.92 KK1796 pKa = 10.41 PLDD1799 pKa = 3.62 SLEE1802 pKa = 4.48 AFLDD1806 pKa = 3.45 AMRR1809 pKa = 11.84 NRR1811 pKa = 11.84 SPQSTTQYY1819 pKa = 10.63 TLLLSDD1825 pKa = 4.12 PVKK1828 pKa = 10.73 PLDD1831 pKa = 3.98 NPSFSYY1837 pKa = 10.81 EE1838 pKa = 3.99 FLNLLVTNLNINKK1851 pKa = 8.88 QFAQLIVNKK1860 pKa = 9.14 YY1861 pKa = 10.2 HH1862 pKa = 5.8 NTCALQSAIQMNTTTDD1878 pKa = 3.09 THH1880 pKa = 6.38 NFLATFIYY1888 pKa = 10.64 LLYY1891 pKa = 10.11 TMPYY1895 pKa = 9.9 SMNTFRR1901 pKa = 11.84 QTHH1904 pKa = 4.87 TPEE1907 pKa = 4.13 EE1908 pKa = 4.56 PFTPGKK1914 pKa = 9.45 MVTVVDD1920 pKa = 3.56 TTIAFLTTLDD1930 pKa = 3.78 ILPPCGQPCGYY1941 pKa = 9.75 LPPSITKK1948 pKa = 10.37 DD1949 pKa = 3.14 GEE1951 pKa = 4.53 YY1952 pKa = 10.01 ICACQKK1958 pKa = 9.04 TSNWSLPFHH1967 pKa = 7.31 FYY1969 pKa = 9.74 NARR1972 pKa = 11.84 YY1973 pKa = 9.41 NKK1975 pKa = 9.81 VYY1977 pKa = 9.0 HH1978 pKa = 6.49 TGLNNILTHH1987 pKa = 5.69 KK1988 pKa = 10.21 HH1989 pKa = 4.45 SAFHH1993 pKa = 6.86 KK1994 pKa = 10.18 SRR1996 pKa = 11.84 NAAHH2000 pKa = 7.31 FIAKK2004 pKa = 8.28 TGPSTSSYY2012 pKa = 8.59 PVYY2015 pKa = 9.49 MAPVPEE2021 pKa = 3.87 ILAYY2025 pKa = 9.51 NASYY2029 pKa = 10.87 RR2030 pKa = 11.84 DD2031 pKa = 3.52 SCQDD2035 pKa = 3.23 NAIEE2039 pKa = 4.2 EE2040 pKa = 4.36 QSDD2043 pKa = 3.86 SQASQSPSSPVTIPVSLPTASPASSVKK2070 pKa = 10.23 SALRR2074 pKa = 11.84 SDD2076 pKa = 3.62 IPITTDD2082 pKa = 3.24 QQSTTSASISTATTASTIPTAPLTSSDD2109 pKa = 3.83 SNTSVVTSLYY2119 pKa = 11.11 GNMEE2123 pKa = 4.28 EE2124 pKa = 4.89 LTYY2127 pKa = 10.96 LDD2129 pKa = 4.84 ASGTSQDD2136 pKa = 4.45 FILSEE2141 pKa = 4.15 TTPFIAHH2148 pKa = 7.21 IYY2150 pKa = 8.98 HH2151 pKa = 6.24 NNEE2154 pKa = 3.46 ATFIPPGYY2162 pKa = 10.06 QLLDD2166 pKa = 3.65 TNTNDD2171 pKa = 4.46 PIEE2174 pKa = 4.33 MYY2176 pKa = 8.76 ITPPRR2181 pKa = 11.84 PIDD2184 pKa = 3.98 GSPMISLASTASTTPMTYY2202 pKa = 9.73 PLLSIRR2208 pKa = 11.84 LTTEE2212 pKa = 3.4 EE2213 pKa = 4.22 LTSFFKK2219 pKa = 10.65 TKK2221 pKa = 8.7 TDD2223 pKa = 3.13 KK2224 pKa = 11.03 FHH2226 pKa = 7.15 LISHH2230 pKa = 6.87 KK2231 pKa = 10.87 SCLTVHH2237 pKa = 6.82 LFDD2240 pKa = 5.49 SPTLNSIAADD2250 pKa = 3.84 STSDD2254 pKa = 2.86 AHH2256 pKa = 7.76 LYY2258 pKa = 8.12 QQHH2261 pKa = 6.78 LKK2263 pKa = 10.78 DD2264 pKa = 4.67 LYY2266 pKa = 10.41 TFSDD2270 pKa = 4.16 CCSMYY2275 pKa = 10.67 TRR2277 pKa = 11.84 TEE2279 pKa = 3.89 VYY2281 pKa = 10.61 NCIEE2285 pKa = 4.65 ADD2287 pKa = 3.58 TPLIRR2292 pKa = 11.84 QSEE2295 pKa = 4.28 QTKK2298 pKa = 8.41 FHH2300 pKa = 7.43 PINLDD2305 pKa = 3.35 TLIEE2309 pKa = 4.17 MVATFPPIVKK2319 pKa = 9.79 RR2320 pKa = 11.84 YY2321 pKa = 9.37 SQTTTPDD2328 pKa = 3.3 FTNLTVYY2335 pKa = 10.04 FVSNGDD2341 pKa = 3.79 IITTPTGSTSEE2352 pKa = 4.19 QPPQLKK2358 pKa = 10.17 IFLDD2362 pKa = 4.01 YY2363 pKa = 9.8 QTSSKK2368 pKa = 9.54 FTTLVDD2374 pKa = 3.96 LTLHH2378 pKa = 5.99 EE2379 pKa = 4.17 QTEE2382 pKa = 4.54 ANTIITYY2389 pKa = 9.6 HH2390 pKa = 6.93 HH2391 pKa = 7.2 GEE2393 pKa = 4.13 HH2394 pKa = 6.25 QLLKK2398 pKa = 9.59 PNPSAFYY2405 pKa = 10.55 IEE2407 pKa = 4.18 FQTYY2411 pKa = 9.89 SSFFSRR2417 pKa = 11.84 FQTFSTNFFWTLFINFLINVRR2438 pKa = 11.84 FCITADD2444 pKa = 2.85 SAYY2447 pKa = 10.34 FHH2449 pKa = 6.57 WQGKK2453 pKa = 8.71 PIEE2456 pKa = 4.38 TTNLNIVYY2464 pKa = 10.39 SIGRR2468 pKa = 11.84 LDD2470 pKa = 3.81 FVLSKK2475 pKa = 9.84 HH2476 pKa = 4.51 TTPWLTKK2483 pKa = 9.31 PTDD2486 pKa = 3.62 TLNPLTLIKK2495 pKa = 9.79 NTLVQPIAINFHH2507 pKa = 4.79 GRR2509 pKa = 11.84 IRR2511 pKa = 11.84 PLQSTNTRR2519 pKa = 11.84 FGATHH2524 pKa = 7.26 TPTKK2528 pKa = 10.77 LPVHH2532 pKa = 6.74 LLNTSLRR2539 pKa = 11.84 THH2541 pKa = 5.98 YY2542 pKa = 10.84 LSLLSLFQCTFSVFLAYY2559 pKa = 9.39 IALLYY2564 pKa = 10.57 SFSGHH2569 pKa = 7.13 GIFTVVAYY2577 pKa = 7.97 FTMLFARR2584 pKa = 11.84 YY2585 pKa = 9.5 YY2586 pKa = 7.83 ITSFINFCTSQLTATQVTQWFAAIKK2611 pKa = 10.18 AKK2613 pKa = 9.51 YY2614 pKa = 7.51 TGIYY2618 pKa = 9.7 EE2619 pKa = 4.2 SSQDD2623 pKa = 3.05 RR2624 pKa = 11.84 VLTVNVTGTNVPYY2637 pKa = 9.92 IVKK2640 pKa = 10.12 YY2641 pKa = 8.88 STILTVTMYY2650 pKa = 10.11 VAFMAFVWTVSTYY2663 pKa = 10.49 AAQYY2667 pKa = 8.11 TAGEE2671 pKa = 4.42 RR2672 pKa = 11.84 YY2673 pKa = 9.7 DD2674 pKa = 3.64 RR2675 pKa = 11.84 PPYY2678 pKa = 8.97 QTVFQKK2684 pKa = 9.63 TLNVLGLTEE2693 pKa = 4.33 TVTYY2697 pKa = 8.58 YY2698 pKa = 11.02 YY2699 pKa = 10.14 PYY2701 pKa = 10.98 ASLNEE2706 pKa = 3.75 ACAASTSILCRR2717 pKa = 11.84 LGSPFNFHH2725 pKa = 6.48 YY2726 pKa = 10.39 PSDD2729 pKa = 3.94 YY2730 pKa = 9.07 TQVRR2734 pKa = 11.84 TVQTDD2739 pKa = 3.23 TSSPFWLFIIFMPPSFLFIVLPWLILCTITPTVSIAQLLVPSIILNATIVFIYY2792 pKa = 9.95 IRR2794 pKa = 11.84 RR2795 pKa = 11.84 KK2796 pKa = 8.63 FTGHH2800 pKa = 6.35 CCGPHH2805 pKa = 5.06 TCIKK2809 pKa = 10.17 HH2810 pKa = 6.14 ADD2812 pKa = 3.37 ISRR2815 pKa = 11.84 SLQFRR2820 pKa = 11.84 PTSQIQHH2827 pKa = 6.29 SLTFCGTLCAKK2838 pKa = 10.33 HH2839 pKa = 6.16 NWYY2842 pKa = 10.38 CNNSDD2847 pKa = 4.65 SPTHH2851 pKa = 5.46 TLGIQLAQLIEE2862 pKa = 4.33 TTYY2865 pKa = 11.0 KK2866 pKa = 10.37 LQPGTIKK2873 pKa = 10.32 PDD2875 pKa = 2.82 SSYY2878 pKa = 9.92 THH2880 pKa = 5.25 TTEE2883 pKa = 4.04 TATLPIMKK2891 pKa = 9.7 VSTTSTDD2898 pKa = 3.66 FTTSEE2903 pKa = 4.06 PNTTVEE2909 pKa = 4.14 HH2910 pKa = 6.25 LHH2912 pKa = 6.0 LQVIAHH2918 pKa = 4.87 VTGTRR2923 pKa = 11.84 ISIEE2927 pKa = 3.93 SSSNKK2932 pKa = 9.2 VQQQNTQHH2940 pKa = 5.73 TRR2942 pKa = 11.84 LTNKK2946 pKa = 9.47 PVTGFMHH2953 pKa = 5.71 TTLLQKK2959 pKa = 10.51 LKK2961 pKa = 10.22 RR2962 pKa = 11.84 QHH2964 pKa = 6.6 KK2965 pKa = 10.21 DD2966 pKa = 2.92 EE2967 pKa = 4.72 LSSYY2971 pKa = 10.3 LCNFVPSDD2979 pKa = 3.64 NKK2981 pKa = 10.32 KK2982 pKa = 10.86 DD2983 pKa = 3.94 CILPHH2988 pKa = 6.24 SVVHH2992 pKa = 5.26 MTLTEE2997 pKa = 3.94 NQRR3000 pKa = 11.84 TFLLKK3005 pKa = 10.87 NFTFSTNVTVDD3016 pKa = 3.16 PTTTGFIPSSLNISTLPHH3034 pKa = 5.35 KK3035 pKa = 10.32 HH3036 pKa = 5.7 FMINVIEE3043 pKa = 4.24 SAMLAKK3049 pKa = 10.28 LPKK3052 pKa = 9.73 EE3053 pKa = 4.27 VQDD3056 pKa = 3.91 TLRR3059 pKa = 11.84 TTHH3062 pKa = 7.25 LEE3064 pKa = 4.03 TTTLEE3069 pKa = 4.05 RR3070 pKa = 11.84 QAMSLTTQAILTTFALIMATFVVAFLAFFSTAQVGKK3106 pKa = 8.47 TPYY3109 pKa = 10.56 AGLNPTMVGNVNAEE3123 pKa = 4.4 PYY3125 pKa = 9.48 IQPTTLEE3132 pKa = 3.96 NSILIPLHH3140 pKa = 5.98 GASKK3144 pKa = 10.2 VCWRR3148 pKa = 11.84 AQNGTLFFTDD3158 pKa = 5.58 AIPTTEE3164 pKa = 4.34 CARR3167 pKa = 11.84 AAVPYY3172 pKa = 9.82 IGYY3175 pKa = 9.97 KK3176 pKa = 9.89 SEE3178 pKa = 4.03 FTQTCASSNLRR3189 pKa = 11.84 YY3190 pKa = 9.68 PFTVYY3195 pKa = 10.52 LGSIKK3200 pKa = 10.62 VMYY3203 pKa = 10.33 LRR3205 pKa = 11.84 DD3206 pKa = 4.43 GISYY3210 pKa = 7.59 LTSTLSHH3217 pKa = 6.49 NSNTKK3222 pKa = 9.71 KK3223 pKa = 10.7 LCVQVGSNAVRR3234 pKa = 11.84 CASVLPTGASSNVAALLMASVVVISMVLFYY3264 pKa = 10.92 LYY3266 pKa = 10.76 LLQIFKK3272 pKa = 10.73 FYY3274 pKa = 9.4 TNSVIMSFVIQLLTLLATTVSTPLAVTVQLFVITYY3309 pKa = 9.58 GYY3311 pKa = 8.65 TNWILLTLSLLNLTVLLSTPVGITFVVIYY3340 pKa = 9.86 GLYY3343 pKa = 9.77 KK3344 pKa = 10.57 AYY3346 pKa = 9.29 TLFTSSGQGCVYY3358 pKa = 10.96 NEE3360 pKa = 3.97 GGTIRR3365 pKa = 11.84 FSGSFEE3371 pKa = 3.82 QVANSTFPLTNASCVQLLSDD3391 pKa = 5.56 LGITYY3396 pKa = 9.62 QQLNVYY3402 pKa = 10.22 ASSRR3406 pKa = 11.84 DD3407 pKa = 3.46 RR3408 pKa = 11.84 NVRR3411 pKa = 11.84 RR3412 pKa = 11.84 LAQALLHH3419 pKa = 6.15 RR3420 pKa = 11.84 QLDD3423 pKa = 4.06 SASEE3427 pKa = 4.29 CILYY3431 pKa = 9.59 EE3432 pKa = 4.31 GCSGNTITRR3441 pKa = 11.84 QALQRR3446 pKa = 11.84 IRR3448 pKa = 11.84 QAVTVVVTPASQNLCKK3464 pKa = 9.54 ITSNQANGIGLSCTGTFFTSTEE3486 pKa = 4.3 IITCAHH3492 pKa = 7.36 GIGTSDD3498 pKa = 2.61 ITAVHH3503 pKa = 6.49 KK3504 pKa = 11.01 GITYY3508 pKa = 9.18 DD3509 pKa = 4.41 CKK3511 pKa = 11.09 VKK3513 pKa = 10.82 SINNDD3518 pKa = 2.48 IAILITTTVNLSVQNIKK3535 pKa = 10.52 LDD3537 pKa = 3.56 SSFSQKK3543 pKa = 10.25 SDD3545 pKa = 2.5 NYY3547 pKa = 10.19 QRR3549 pKa = 11.84 NFVQFVSFVDD3559 pKa = 3.77 QQNSDD3564 pKa = 3.08 AVTINNTVMLPSGHH3578 pKa = 6.26 FFAIGTEE3585 pKa = 4.07 AGEE3588 pKa = 4.38 SGSPYY3593 pKa = 9.57 TLNGNIIGIHH3603 pKa = 5.5 YY3604 pKa = 10.26 GIDD3607 pKa = 3.54 NAGSWMLASRR3617 pKa = 11.84 PDD3619 pKa = 3.21 GSFYY3623 pKa = 11.39 VPATQHH3629 pKa = 5.83 GNSAKK3634 pKa = 9.35 VTFSTDD3640 pKa = 2.22 AFAQQFPAIVTNKK3653 pKa = 9.86 TSDD3656 pKa = 3.31 QLVNEE3661 pKa = 4.55 IAATNNTAFEE3671 pKa = 5.11 LDD3673 pKa = 3.63 TTDD3676 pKa = 4.18 VSHH3679 pKa = 7.67 LSNLLKK3685 pKa = 10.47 HH3686 pKa = 6.52 LKK3688 pKa = 10.21 NNNEE3692 pKa = 4.29 TPKK3695 pKa = 10.73 SLSDD3699 pKa = 3.97 YY3700 pKa = 10.99 LPVAPVTQQTSVTVGQTLTSPVNNMQTALYY3730 pKa = 9.19 TLMLVSEE3737 pKa = 4.94 VITYY3741 pKa = 10.57 ILTPNSDD3748 pKa = 3.73 LSVLISMFVTSAFLKK3763 pKa = 10.65 FGASKK3768 pKa = 10.8 LFYY3771 pKa = 8.75 NTEE3774 pKa = 3.94 MLRR3777 pKa = 11.84 NTITTFVVYY3786 pKa = 10.11 RR3787 pKa = 11.84 YY3788 pKa = 5.88 TTLLIALVFSQYY3800 pKa = 10.78 YY3801 pKa = 9.4 LHH3803 pKa = 6.81 ILSYY3807 pKa = 10.85 ALKK3810 pKa = 10.74 LNTLVLTVIALTFLVTPLVLLTIRR3834 pKa = 11.84 RR3835 pKa = 11.84 VYY3837 pKa = 10.65 YY3838 pKa = 9.87 YY3839 pKa = 10.11 SQNYY3843 pKa = 9.69 LISCVFIISCFASHH3857 pKa = 7.31 TYY3859 pKa = 8.88 TLYY3862 pKa = 10.49 ILTDD3866 pKa = 3.45 TTVDD3870 pKa = 3.86 FQTFIISEE3878 pKa = 4.69 PIFSTLLCNLFIGFTLISVVPNPLYY3903 pKa = 9.33 VCVVFMYY3910 pKa = 10.65 ILLDD3914 pKa = 3.78 CEE3916 pKa = 3.99 ALGFIVTCFMASYY3929 pKa = 10.19 LCPKK3933 pKa = 9.31 PLRR3936 pKa = 11.84 SLTTFLCTDD3945 pKa = 4.04 TLVLTAPAYY3954 pKa = 8.6 LHH3956 pKa = 6.77 WYY3958 pKa = 7.94 GAKK3961 pKa = 8.77 GTQRR3965 pKa = 11.84 EE3966 pKa = 4.12 YY3967 pKa = 11.05 SVIYY3971 pKa = 10.46 AVFDD3975 pKa = 4.21 SILTPEE3981 pKa = 4.21 TTIQIPVTIMEE3992 pKa = 5.18 GIEE3995 pKa = 3.9 QQVKK3999 pKa = 9.91 FIFAVPKK4006 pKa = 9.87 SANIQDD4012 pKa = 3.66 EE4013 pKa = 4.38 EE4014 pKa = 4.14 EE4015 pKa = 5.08 AYY4017 pKa = 10.83 VEE4019 pKa = 4.2 YY4020 pKa = 10.84 NQNSDD4025 pKa = 3.64 IKK4027 pKa = 11.09 DD4028 pKa = 3.41 LAIKK4032 pKa = 10.64 NEE4034 pKa = 4.15 EE4035 pKa = 4.19 KK4036 pKa = 10.82 VCTITGMFRR4045 pKa = 11.84 KK4046 pKa = 7.96 TRR4048 pKa = 11.84 TIKK4051 pKa = 9.32 GTLMEE4056 pKa = 4.39 SFYY4059 pKa = 10.6 PRR4061 pKa = 11.84 HH4062 pKa = 5.68 QPDD4065 pKa = 3.79 SYY4067 pKa = 11.3 ILNQVVRR4074 pKa = 11.84 HH4075 pKa = 5.75 NYY4077 pKa = 8.97 VLAHH4081 pKa = 6.76 DD4082 pKa = 4.93 PEE4084 pKa = 4.95 TIILTTVNPTHH4095 pKa = 6.88 LEE4097 pKa = 3.97 SEE4099 pKa = 4.4 PFQTIVKK4106 pKa = 9.88 KK4107 pKa = 10.31 VRR4109 pKa = 11.84 KK4110 pKa = 9.04 LHH4112 pKa = 6.55 ALYY4115 pKa = 10.56 QEE4117 pKa = 4.39 ISEE4120 pKa = 4.22 QSSDD4124 pKa = 3.81 DD4125 pKa = 3.72 TEE4127 pKa = 4.53 LCHH4130 pKa = 7.8 AYY4132 pKa = 9.45 ILALIKK4138 pKa = 10.25 STVLEE4143 pKa = 4.12 AQMPTEE4149 pKa = 4.7 KK4150 pKa = 10.46 INFVSSQTMLSPAMILIFAEE4170 pKa = 5.22 AYY4172 pKa = 10.21 QILTEE4177 pKa = 4.31 SRR4179 pKa = 11.84 SFTNHH4184 pKa = 5.39 ITPQSDD4190 pKa = 3.0 IGSMQTTLASLAEE4203 pKa = 4.14 MDD4205 pKa = 4.18 TDD4207 pKa = 3.51 EE4208 pKa = 4.64 MTPQEE4213 pKa = 4.18 RR4214 pKa = 11.84 KK4215 pKa = 8.7 IHH4217 pKa = 5.62 IKK4219 pKa = 10.3 RR4220 pKa = 11.84 MNVLKK4225 pKa = 10.84 QEE4227 pKa = 4.02 IAKK4230 pKa = 9.24 MEE4232 pKa = 4.29 SASLKK4237 pKa = 10.64 LEE4239 pKa = 4.04 KK4240 pKa = 10.54 FLDD4243 pKa = 3.88 NMHH4246 pKa = 6.78 KK4247 pKa = 10.93 AEE4249 pKa = 3.68 ISKK4252 pKa = 10.42 RR4253 pKa = 11.84 GKK4255 pKa = 10.27 EE4256 pKa = 4.0 DD4257 pKa = 3.07 ILLKK4261 pKa = 10.01 VSNMLRR4267 pKa = 11.84 LHH4269 pKa = 6.18 LNKK4272 pKa = 9.83 VANAAHH4278 pKa = 6.36 CTIQTPSAGLITLASAFDD4296 pKa = 3.64 VHH4298 pKa = 6.55 SLCVTQHH4305 pKa = 5.85 SEE4307 pKa = 3.98 SVLIQTPDD4315 pKa = 3.4 DD4316 pKa = 3.79 DD4317 pKa = 4.2 TFLVYY4322 pKa = 10.59 VDD4324 pKa = 3.53 GQIYY4328 pKa = 8.39 TCYY4331 pKa = 10.55 NPTDD4335 pKa = 3.34 ITGKK4339 pKa = 10.22 KK4340 pKa = 9.3 LVPINVMSPDD4350 pKa = 3.22 NVQFPTYY4357 pKa = 9.35 PVVFSLSKK4365 pKa = 10.03 QDD4367 pKa = 3.53 YY4368 pKa = 9.89 AEE4370 pKa = 5.28 EE4371 pKa = 3.8 ITEE4374 pKa = 4.08 QNNIGYY4380 pKa = 7.36 TEE4382 pKa = 3.93 RR4383 pKa = 11.84 THH4385 pKa = 6.51 NFKK4388 pKa = 10.85 LKK4390 pKa = 10.26 EE4391 pKa = 4.02 LASGLAVTLDD4401 pKa = 3.39 GLVVVTEE4408 pKa = 4.32 TKK4410 pKa = 10.52 EE4411 pKa = 3.4 LATAFKK4417 pKa = 10.32 IGQRR4421 pKa = 11.84 YY4422 pKa = 8.29 FKK4424 pKa = 10.6 FLNTNKK4430 pKa = 9.6 TPVARR4435 pKa = 11.84 NNTHH4439 pKa = 7.71 AIIQLLRR4446 pKa = 11.84 NNISQQAVVRR4456 pKa = 11.84 IGGSRR4461 pKa = 11.84 VSNDD4465 pKa = 3.81 HH4466 pKa = 6.35 IAISQVPVQTIGYY4479 pKa = 7.18 LTYY4482 pKa = 10.97 AGISVCRR4489 pKa = 11.84 QCATKK4494 pKa = 9.97 QDD4496 pKa = 3.94 HH4497 pKa = 5.48 TCQYY4501 pKa = 10.75 AGYY4504 pKa = 8.56 FVQIPRR4510 pKa = 11.84 EE4511 pKa = 4.02 HH4512 pKa = 6.64 VSNIFNLTDD4521 pKa = 3.58 TPPCLHH4527 pKa = 6.75 NKK4529 pKa = 7.29 FTCTTCQPLQQQSKK4543 pKa = 6.8 TQQPPLNWWGSVV4555 pKa = 3.03
Molecular weight: 508.99 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.897
IPC2_protein 5.97
IPC_protein 6.046
Toseland 6.44
ProMoST 6.465
Dawson 6.389
Bjellqvist 6.389
Wikipedia 6.402
Rodwell 6.376
Grimsley 6.59
Solomon 6.389
Lehninger 6.376
Nozaki 6.678
DTASelect 6.854
Thurlkill 6.883
EMBOSS 6.854
Sillero 6.781
Patrickios 3.516
IPC_peptide 6.402
IPC2_peptide 6.707
IPC2.peptide.svr19 6.67
Protein with the highest isoelectric point:
>sp|Q008X3|VME1_WBV24 Membrane protein OS=White bream virus (isolate Blicca bjoerkna L./Germany/DF24/00) OX=766180 GN=M PE=3 SV=1
MM1 pKa = 7.96 SGMYY5 pKa = 8.01 PQPLMMMPQQQRR17 pKa = 11.84 RR18 pKa = 11.84 PRR20 pKa = 11.84 KK21 pKa = 8.86 PRR23 pKa = 11.84 TKK25 pKa = 10.15 SAPKK29 pKa = 10.13 PPTTSKK35 pKa = 10.29 MSEE38 pKa = 3.9 KK39 pKa = 9.78 TKK41 pKa = 11.06 KK42 pKa = 10.01 EE43 pKa = 4.0 VTKK46 pKa = 10.38 STMSTEE52 pKa = 4.06 KK53 pKa = 10.27 QIPMPTIPAASKK65 pKa = 11.1 GSLKK69 pKa = 11.04 DD70 pKa = 3.26 LDD72 pKa = 3.63 VRR74 pKa = 11.84 NNLNQQEE81 pKa = 4.5 LNGIYY86 pKa = 10.25 YY87 pKa = 8.38 DD88 pKa = 3.79 TVNNLNACHH97 pKa = 6.25 GRR99 pKa = 11.84 MIFQPTGGSLTEE111 pKa = 4.11 GTLTVTIKK119 pKa = 10.86 LRR121 pKa = 11.84 VKK123 pKa = 10.47 SDD125 pKa = 3.1 QCLKK129 pKa = 9.74 MCSAFHH135 pKa = 6.7 KK136 pKa = 10.5 RR137 pKa = 11.84 NTNLEE142 pKa = 4.38 TPLSAVDD149 pKa = 3.3 ISKK152 pKa = 10.66 EE153 pKa = 4.22 SEE155 pKa = 3.81 KK156 pKa = 11.28 SLGKK160 pKa = 10.19 DD161 pKa = 2.84
Molecular weight: 17.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.988
IPC2_protein 9.107
IPC_protein 9.019
Toseland 10.116
ProMoST 9.604
Dawson 10.218
Bjellqvist 9.823
Wikipedia 10.321
Rodwell 10.921
Grimsley 10.248
Solomon 10.248
Lehninger 10.233
Nozaki 10.116
DTASelect 9.794
Thurlkill 10.101
EMBOSS 10.482
Sillero 10.145
Patrickios 10.657
IPC_peptide 10.248
IPC2_peptide 8.331
IPC2.peptide.svr19 8.083
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
13035
161
6872
2607.0
291.58
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.153 ± 0.182
1.857 ± 0.063
3.966 ± 0.165
4.204 ± 0.326
4.703 ± 0.146
3.659 ± 0.257
3.138 ± 0.307
6.651 ± 0.108
4.848 ± 0.302
9.275 ± 0.276
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.703 ± 0.274
5.339 ± 0.118
6.038 ± 0.181
4.956 ± 0.282
2.639 ± 0.142
7.679 ± 0.339
11.845 ± 0.545
6.214 ± 0.158
0.644 ± 0.025
4.488 ± 0.178
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here