Amino acid dipepetide frequency for Entamoeba invadens IP1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.081AlaAla: 2.081 ± 0.037
1.037AlaCys: 1.037 ± 0.02
2.101AlaAsp: 2.101 ± 0.029
2.752AlaGlu: 2.752 ± 0.036
2.535AlaPhe: 2.535 ± 0.031
1.695AlaGly: 1.695 ± 0.03
0.641AlaHis: 0.641 ± 0.011
3.051AlaIle: 3.051 ± 0.029
3.506AlaLys: 3.506 ± 0.039
4.056AlaLeu: 4.056 ± 0.04
1.111AlaMet: 1.111 ± 0.017
2.289AlaAsn: 2.289 ± 0.028
1.47AlaPro: 1.47 ± 0.024
1.624AlaGln: 1.624 ± 0.021
1.267AlaArg: 1.267 ± 0.02
2.614AlaSer: 2.614 ± 0.029
2.669AlaThr: 2.669 ± 0.031
2.871AlaVal: 2.871 ± 0.033
0.283AlaTrp: 0.283 ± 0.008
1.213AlaTyr: 1.213 ± 0.018
0.0AlaXaa: 0.0 ± 0.0
Cys
1.463CysAla: 1.463 ± 0.031
0.642CysCys: 0.642 ± 0.017
2.433CysAsp: 2.433 ± 0.05
2.607CysGlu: 2.607 ± 0.049
2.048CysPhe: 2.048 ± 0.037
1.731CysGly: 1.731 ± 0.027
0.469CysHis: 0.469 ± 0.012
2.101CysIle: 2.101 ± 0.037
2.581CysLys: 2.581 ± 0.049
2.726CysLeu: 2.726 ± 0.041
0.587CysMet: 0.587 ± 0.012
1.968CysAsn: 1.968 ± 0.044
1.01CysPro: 1.01 ± 0.024
1.226CysGln: 1.226 ± 0.031
0.776CysArg: 0.776 ± 0.014
3.054CysSer: 3.054 ± 0.067
2.519CysThr: 2.519 ± 0.056
3.177CysVal: 3.177 ± 0.057
0.238CysTrp: 0.238 ± 0.007
1.293CysTyr: 1.293 ± 0.023
0.0CysXaa: 0.0 ± 0.0
Asp
2.35AspAla: 2.35 ± 0.027
1.431AspCys: 1.431 ± 0.023
3.326AspAsp: 3.326 ± 0.036
4.243AspGlu: 4.243 ± 0.041
3.135AspPhe: 3.135 ± 0.029
3.222AspGly: 3.222 ± 0.036
0.708AspHis: 0.708 ± 0.013
4.032AspIle: 4.032 ± 0.031
4.353AspLys: 4.353 ± 0.039
4.411AspLeu: 4.411 ± 0.035
1.265AspMet: 1.265 ± 0.019
3.357AspAsn: 3.357 ± 0.033
1.487AspPro: 1.487 ± 0.02
1.643AspGln: 1.643 ± 0.021
1.47AspArg: 1.47 ± 0.02
3.497AspSer: 3.497 ± 0.034
3.196AspThr: 3.196 ± 0.032
4.106AspVal: 4.106 ± 0.033
0.392AspTrp: 0.392 ± 0.009
2.086AspTyr: 2.086 ± 0.024
0.0AspXaa: 0.0 ± 0.0
Glu
2.756GluAla: 2.756 ± 0.034
2.345GluCys: 2.345 ± 0.039
3.709GluAsp: 3.709 ± 0.036
6.298GluGlu: 6.298 ± 0.079
3.089GluPhe: 3.089 ± 0.029
2.988GluGly: 2.988 ± 0.031
1.059GluHis: 1.059 ± 0.019
5.398GluIle: 5.398 ± 0.047
8.043GluLys: 8.043 ± 0.087
5.431GluLeu: 5.431 ± 0.045
2.23GluMet: 2.23 ± 0.021
5.246GluAsn: 5.246 ± 0.055
1.439GluPro: 1.439 ± 0.02
2.49GluGln: 2.49 ± 0.031
2.76GluArg: 2.76 ± 0.037
4.356GluSer: 4.356 ± 0.032
4.311GluThr: 4.311 ± 0.032
4.467GluVal: 4.467 ± 0.039
0.488GluTrp: 0.488 ± 0.011
2.411GluTyr: 2.411 ± 0.026
0.0GluXaa: 0.0 ± 0.0
Phe
2.277PheAla: 2.277 ± 0.024
1.427PheCys: 1.427 ± 0.02
3.366PheAsp: 3.366 ± 0.028
3.813PheGlu: 3.813 ± 0.036
2.589PhePhe: 2.589 ± 0.032
3.194PheGly: 3.194 ± 0.035
0.883PheHis: 0.883 ± 0.015
3.386PheIle: 3.386 ± 0.033
3.666PheLys: 3.666 ± 0.031
4.194PheLeu: 4.194 ± 0.042
1.153PheMet: 1.153 ± 0.017
3.063PheAsn: 3.063 ± 0.026
1.833PhePro: 1.833 ± 0.023
1.567PheGln: 1.567 ± 0.019
1.569PheArg: 1.569 ± 0.019
4.088PheSer: 4.088 ± 0.032
2.979PheThr: 2.979 ± 0.027
4.069PheVal: 4.069 ± 0.042
0.403PheTrp: 0.403 ± 0.009
2.251PheTyr: 2.251 ± 0.026
0.0PheXaa: 0.0 ± 0.0
Gly
1.976GlyAla: 1.976 ± 0.029
2.109GlyCys: 2.109 ± 0.032
2.613GlyAsp: 2.613 ± 0.028
3.052GlyGlu: 3.052 ± 0.028
2.348GlyPhe: 2.348 ± 0.025
2.34GlyGly: 2.34 ± 0.037
0.655GlyHis: 0.655 ± 0.014
3.446GlyIle: 3.446 ± 0.032
4.203GlyLys: 4.203 ± 0.036
3.177GlyLeu: 3.177 ± 0.037
1.184GlyMet: 1.184 ± 0.019
3.108GlyAsn: 3.108 ± 0.032
0.713GlyPro: 0.713 ± 0.014
1.268GlyGln: 1.268 ± 0.024
1.463GlyArg: 1.463 ± 0.022
3.138GlySer: 3.138 ± 0.037
2.902GlyThr: 2.902 ± 0.038
3.586GlyVal: 3.586 ± 0.032
0.403GlyTrp: 0.403 ± 0.01
2.579GlyTyr: 2.579 ± 0.038
0.0GlyXaa: 0.0 ± 0.0
His
0.579HisAla: 0.579 ± 0.014
0.681HisCys: 0.681 ± 0.017
0.694HisAsp: 0.694 ± 0.014
0.894HisGlu: 0.894 ± 0.014
1.212HisPhe: 1.212 ± 0.019
0.614HisGly: 0.614 ± 0.013
0.393HisHis: 0.393 ± 0.014
1.25HisIle: 1.25 ± 0.02
1.193HisLys: 1.193 ± 0.018
1.582HisLeu: 1.582 ± 0.02
0.356HisMet: 0.356 ± 0.009
0.926HisAsn: 0.926 ± 0.016
0.717HisPro: 0.717 ± 0.014
0.597HisGln: 0.597 ± 0.014
0.686HisArg: 0.686 ± 0.012
1.146HisSer: 1.146 ± 0.017
0.948HisThr: 0.948 ± 0.016
1.029HisVal: 1.029 ± 0.017
0.125HisTrp: 0.125 ± 0.006
0.655HisTyr: 0.655 ± 0.014
0.0HisXaa: 0.0 ± 0.0
Ile
3.112IleAla: 3.112 ± 0.033
1.933IleCys: 1.933 ± 0.033
4.032IleAsp: 4.032 ± 0.035
5.464IleGlu: 5.464 ± 0.046
3.206IlePhe: 3.206 ± 0.033
3.67IleGly: 3.67 ± 0.035
1.22IleHis: 1.22 ± 0.018
4.374IleIle: 4.374 ± 0.034
5.527IleLys: 5.527 ± 0.042
5.471IleLeu: 5.471 ± 0.041
1.27IleMet: 1.27 ± 0.02
4.3IleAsn: 4.3 ± 0.038
3.496IlePro: 3.496 ± 0.041
2.771IleGln: 2.771 ± 0.03
2.181IleArg: 2.181 ± 0.024
5.249IleSer: 5.249 ± 0.04
4.773IleThr: 4.773 ± 0.042
4.925IleVal: 4.925 ± 0.037
0.418IleTrp: 0.418 ± 0.01
2.203IleTyr: 2.203 ± 0.026
0.0IleXaa: 0.0 ± 0.0
Lys
3.369LysAla: 3.369 ± 0.044
4.15LysCys: 4.15 ± 0.082
4.307LysAsp: 4.307 ± 0.038
7.394LysGlu: 7.394 ± 0.076
3.293LysPhe: 3.293 ± 0.029
3.465LysGly: 3.465 ± 0.031
1.316LysHis: 1.316 ± 0.018
5.837LysIle: 5.837 ± 0.044
8.326LysLys: 8.326 ± 0.088
6.361LysLeu: 6.361 ± 0.051
2.684LysMet: 2.684 ± 0.027
5.354LysAsn: 5.354 ± 0.041
2.272LysPro: 2.272 ± 0.028
3.059LysGln: 3.059 ± 0.038
3.432LysArg: 3.432 ± 0.037
5.559LysSer: 5.559 ± 0.041
6.019LysThr: 6.019 ± 0.045
5.228LysVal: 5.228 ± 0.045
0.56LysTrp: 0.56 ± 0.011
3.531LysTyr: 3.531 ± 0.034
0.0LysXaa: 0.0 ± 0.0
Leu
3.173LeuAla: 3.173 ± 0.036
2.412LeuCys: 2.412 ± 0.034
4.423LeuAsp: 4.423 ± 0.037
5.541LeuGlu: 5.541 ± 0.049
4.719LeuPhe: 4.719 ± 0.048
3.253LeuGly: 3.253 ± 0.032
1.719LeuHis: 1.719 ± 0.023
5.386LeuIle: 5.386 ± 0.044
7.521LeuLys: 7.521 ± 0.056
7.528LeuLeu: 7.528 ± 0.061
2.045LeuMet: 2.045 ± 0.022
5.297LeuAsn: 5.297 ± 0.045
3.375LeuPro: 3.375 ± 0.037
3.311LeuGln: 3.311 ± 0.032
3.164LeuArg: 3.164 ± 0.029
6.951LeuSer: 6.951 ± 0.048
5.36LeuThr: 5.36 ± 0.042
5.393LeuVal: 5.393 ± 0.046
0.559LeuTrp: 0.559 ± 0.013
3.023LeuTyr: 3.023 ± 0.032
0.0LeuXaa: 0.0 ± 0.0
Met
1.15MetAla: 1.15 ± 0.017
0.826MetCys: 0.826 ± 0.017
1.12MetAsp: 1.12 ± 0.017
1.69MetGlu: 1.69 ± 0.021
1.225MetPhe: 1.225 ± 0.019
0.982MetGly: 0.982 ± 0.018
0.29MetHis: 0.29 ± 0.007
1.42MetIle: 1.42 ± 0.018
2.611MetLys: 2.611 ± 0.026
1.959MetLeu: 1.959 ± 0.02
0.752MetMet: 0.752 ± 0.016
1.532MetAsn: 1.532 ± 0.018
0.673MetPro: 0.673 ± 0.014
0.738MetGln: 0.738 ± 0.014
0.932MetArg: 0.932 ± 0.017
2.095MetSer: 2.095 ± 0.025
1.773MetThr: 1.773 ± 0.019
1.407MetVal: 1.407 ± 0.018
0.219MetTrp: 0.219 ± 0.006
0.967MetTyr: 0.967 ± 0.015
0.0MetXaa: 0.0 ± 0.0
Asn
2.676AsnAla: 2.676 ± 0.025
3.091AsnCys: 3.091 ± 0.063
3.257AsnAsp: 3.257 ± 0.029
4.738AsnGlu: 4.738 ± 0.052
2.833AsnPhe: 2.833 ± 0.033
3.936AsnGly: 3.936 ± 0.043
0.838AsnHis: 0.838 ± 0.014
4.528AsnIle: 4.528 ± 0.035
5.027AsnLys: 5.027 ± 0.043
4.756AsnLeu: 4.756 ± 0.04
1.639AsnMet: 1.639 ± 0.02
4.291AsnAsn: 4.291 ± 0.044
1.939AsnPro: 1.939 ± 0.023
1.862AsnGln: 1.862 ± 0.022
1.694AsnArg: 1.694 ± 0.021
4.519AsnSer: 4.519 ± 0.037
4.632AsnThr: 4.632 ± 0.041
4.473AsnVal: 4.473 ± 0.037
0.419AsnTrp: 0.419 ± 0.012
2.629AsnTyr: 2.629 ± 0.03
0.0AsnXaa: 0.0 ± 0.0
Pro
1.094ProAla: 1.094 ± 0.017
0.614ProCys: 0.614 ± 0.015
1.66ProAsp: 1.66 ± 0.02
2.309ProGlu: 2.309 ± 0.025
1.726ProPhe: 1.726 ± 0.02
0.941ProGly: 0.941 ± 0.02
0.656ProHis: 0.656 ± 0.012
2.418ProIle: 2.418 ± 0.025
2.896ProLys: 2.896 ± 0.03
3.015ProLeu: 3.015 ± 0.03
0.672ProMet: 0.672 ± 0.012
2.345ProAsn: 2.345 ± 0.025
1.666ProPro: 1.666 ± 0.039
1.808ProGln: 1.808 ± 0.026
1.075ProArg: 1.075 ± 0.02
2.847ProSer: 2.847 ± 0.031
2.426ProThr: 2.426 ± 0.03
1.961ProVal: 1.961 ± 0.024
0.23ProTrp: 0.23 ± 0.007
1.104ProTyr: 1.104 ± 0.018
0.0ProXaa: 0.0 ± 0.0
Gln
1.251GlnAla: 1.251 ± 0.02
1.324GlnCys: 1.324 ± 0.029
1.483GlnAsp: 1.483 ± 0.015
2.078GlnGlu: 2.078 ± 0.025
1.834GlnPhe: 1.834 ± 0.021
1.013GlnGly: 1.013 ± 0.017
0.683GlnHis: 0.683 ± 0.013
3.025GlnIle: 3.025 ± 0.03
3.479GlnLys: 3.479 ± 0.04
3.164GlnLeu: 3.164 ± 0.032
1.147GlnMet: 1.147 ± 0.019
2.931GlnAsn: 2.931 ± 0.031
1.282GlnPro: 1.282 ± 0.024
2.201GlnGln: 2.201 ± 0.047
1.535GlnArg: 1.535 ± 0.021
2.493GlnSer: 2.493 ± 0.026
2.604GlnThr: 2.604 ± 0.026
1.83GlnVal: 1.83 ± 0.023
0.241GlnTrp: 0.241 ± 0.008
1.338GlnTyr: 1.338 ± 0.019
0.0GlnXaa: 0.0 ± 0.0
Arg
1.369ArgAla: 1.369 ± 0.021
1.221ArgCys: 1.221 ± 0.023
1.796ArgAsp: 1.796 ± 0.024
2.339ArgGlu: 2.339 ± 0.031
1.498ArgPhe: 1.498 ± 0.02
1.633ArgGly: 1.633 ± 0.021
0.528ArgHis: 0.528 ± 0.012
2.362ArgIle: 2.362 ± 0.026
2.938ArgLys: 2.938 ± 0.035
2.937ArgLeu: 2.937 ± 0.028
0.867ArgMet: 0.867 ± 0.015
2.114ArgAsn: 2.114 ± 0.021
1.028ArgPro: 1.028 ± 0.019
1.128ArgGln: 1.128 ± 0.019
1.672ArgArg: 1.672 ± 0.028
2.148ArgSer: 2.148 ± 0.022
1.817ArgThr: 1.817 ± 0.024
2.445ArgVal: 2.445 ± 0.027
0.256ArgTrp: 0.256 ± 0.008
1.3ArgTyr: 1.3 ± 0.02
0.0ArgXaa: 0.0 ± 0.0
Ser
3.02SerAla: 3.02 ± 0.03
2.948SerCys: 2.948 ± 0.072
3.924SerAsp: 3.924 ± 0.031
4.672SerGlu: 4.672 ± 0.032
4.019SerPhe: 4.019 ± 0.035
3.757SerGly: 3.757 ± 0.042
1.194SerHis: 1.194 ± 0.019
4.952SerIle: 4.952 ± 0.04
5.515SerLys: 5.515 ± 0.042
6.949SerLeu: 6.949 ± 0.051
1.402SerMet: 1.402 ± 0.018
4.51SerAsn: 4.51 ± 0.045
2.409SerPro: 2.409 ± 0.026
2.869SerGln: 2.869 ± 0.03
2.208SerArg: 2.208 ± 0.026
6.436SerSer: 6.436 ± 0.063
4.909SerThr: 4.909 ± 0.05
5.39SerVal: 5.39 ± 0.053
0.414SerTrp: 0.414 ± 0.01
2.472SerTyr: 2.472 ± 0.025
0.0SerXaa: 0.0 ± 0.0
Thr
2.582ThrAla: 2.582 ± 0.029
2.677ThrCys: 2.677 ± 0.067
2.979ThrAsp: 2.979 ± 0.03
4.188ThrGlu: 4.188 ± 0.034
3.631ThrPhe: 3.631 ± 0.034
2.456ThrGly: 2.456 ± 0.034
1.16ThrHis: 1.16 ± 0.017
4.719ThrIle: 4.719 ± 0.037
5.655ThrLys: 5.655 ± 0.044
6.093ThrLeu: 6.093 ± 0.049
1.263ThrMet: 1.263 ± 0.018
4.376ThrAsn: 4.376 ± 0.045
2.827ThrPro: 2.827 ± 0.032
2.985ThrGln: 2.985 ± 0.032
2.026ThrArg: 2.026 ± 0.023
5.248ThrSer: 5.248 ± 0.044
5.115ThrThr: 5.115 ± 0.053
3.832ThrVal: 3.832 ± 0.035
0.365ThrTrp: 0.365 ± 0.01
2.17ThrTyr: 2.17 ± 0.025
0.0ThrXaa: 0.0 ± 0.0
Val
2.895ValAla: 2.895 ± 0.032
2.219ValCys: 2.219 ± 0.03
3.961ValAsp: 3.961 ± 0.035
4.728ValGlu: 4.728 ± 0.041
4.103ValPhe: 4.103 ± 0.035
3.158ValGly: 3.158 ± 0.03
1.074ValHis: 1.074 ± 0.014
4.817ValIle: 4.817 ± 0.036
5.05ValLys: 5.05 ± 0.038
6.344ValLeu: 6.344 ± 0.046
1.634ValMet: 1.634 ± 0.022
3.92ValAsn: 3.92 ± 0.031
2.297ValPro: 2.297 ± 0.023
2.211ValGln: 2.211 ± 0.025
2.008ValArg: 2.008 ± 0.025
4.99ValSer: 4.99 ± 0.038
4.55ValThr: 4.55 ± 0.041
5.558ValVal: 5.558 ± 0.054
0.52ValTrp: 0.52 ± 0.011
2.456ValTyr: 2.456 ± 0.026
0.0ValXaa: 0.0 ± 0.0
Trp
0.291TrpAla: 0.291 ± 0.009
0.26TrpCys: 0.26 ± 0.008
0.443TrpAsp: 0.443 ± 0.012
0.438TrpGlu: 0.438 ± 0.012
0.384TrpPhe: 0.384 ± 0.009
0.364TrpGly: 0.364 ± 0.011
0.108TrpHis: 0.108 ± 0.005
0.426TrpIle: 0.426 ± 0.01
0.679TrpLys: 0.679 ± 0.013
0.497TrpLeu: 0.497 ± 0.011
0.222TrpMet: 0.222 ± 0.007
0.441TrpAsn: 0.441 ± 0.01
0.133TrpPro: 0.133 ± 0.006
0.172TrpGln: 0.172 ± 0.007
0.276TrpArg: 0.276 ± 0.008
0.482TrpSer: 0.482 ± 0.011
0.38TrpThr: 0.38 ± 0.01
0.483TrpVal: 0.483 ± 0.011
0.123TrpTrp: 0.123 ± 0.006
0.319TrpTyr: 0.319 ± 0.008
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.403TyrAla: 1.403 ± 0.019
1.301TyrCys: 1.301 ± 0.023
2.083TyrAsp: 2.083 ± 0.022
2.079TyrGlu: 2.079 ± 0.023
2.342TyrPhe: 2.342 ± 0.03
1.852TyrGly: 1.852 ± 0.023
0.681TyrHis: 0.681 ± 0.013
2.668TyrIle: 2.668 ± 0.028
2.749TyrLys: 2.749 ± 0.029
3.505TyrLeu: 3.505 ± 0.029
0.875TyrMet: 0.875 ± 0.013
2.407TyrAsn: 2.407 ± 0.028
1.394TyrPro: 1.394 ± 0.024
1.403TyrGln: 1.403 ± 0.021
1.205TyrArg: 1.205 ± 0.019
3.06TyrSer: 3.06 ± 0.036
2.467TyrThr: 2.467 ± 0.029
2.261TyrVal: 2.261 ± 0.025
0.265TyrTrp: 0.265 ± 0.008
2.222TyrTyr: 2.222 ± 0.03
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 9857 proteins (4270709 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski