Vibrio sp. JCM 19236
Average proteome isoelectric point is 6.12
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6630 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0B8PPH2|A0A0B8PPH2_9VIBR Multidrug resistance transporter OS=Vibrio sp. JCM 19236 OX=1481926 GN=JCM19236_4054 PE=4 SV=1
MM1 pKa = 7.34 ATFGADD7 pKa = 2.87 SADD10 pKa = 2.82 IVAGNKK16 pKa = 9.71 DD17 pKa = 3.13 KK18 pKa = 11.14 RR19 pKa = 11.84 SNNFLYY25 pKa = 10.61 AGEE28 pKa = 4.07 FGGLNVQANYY38 pKa = 9.69 IASDD42 pKa = 3.58 EE43 pKa = 4.42 KK44 pKa = 11.31 DD45 pKa = 3.09 ADD47 pKa = 3.86 SYY49 pKa = 11.58 GISALYY55 pKa = 10.65 GFDD58 pKa = 3.66 FGLDD62 pKa = 3.25 LGLGYY67 pKa = 10.64 VGQTNGDD74 pKa = 3.94 DD75 pKa = 5.16 DD76 pKa = 5.43 DD77 pKa = 4.67 SQINFAAQYY86 pKa = 10.32 SIADD90 pKa = 3.59 FTLGGFYY97 pKa = 10.97 GLGSVADD104 pKa = 3.83 EE105 pKa = 4.43 DD106 pKa = 4.4 VNAFEE111 pKa = 5.63 ISALYY116 pKa = 10.06 KK117 pKa = 9.63 LNKK120 pKa = 6.76 WTFVAVYY127 pKa = 9.89 NYY129 pKa = 10.84 NDD131 pKa = 3.36 VDD133 pKa = 4.06 TLIKK137 pKa = 9.6 QAKK140 pKa = 7.74 TGLDD144 pKa = 3.37 EE145 pKa = 4.32 VDD147 pKa = 3.35 NFAIEE152 pKa = 4.08 AVYY155 pKa = 10.5 KK156 pKa = 10.83 FNGNLRR162 pKa = 11.84 TYY164 pKa = 10.76 AGYY167 pKa = 10.67 KK168 pKa = 9.78 FEE170 pKa = 5.15 QVDD173 pKa = 3.69 NQDD176 pKa = 3.77 DD177 pKa = 3.95 QIQAGIRR184 pKa = 11.84 YY185 pKa = 9.42 DD186 pKa = 3.51 FF187 pKa = 4.74
Molecular weight: 20.54 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.687
IPC2_protein 3.795
IPC_protein 3.808
Toseland 3.579
ProMoST 3.999
Dawson 3.821
Bjellqvist 3.973
Wikipedia 3.783
Rodwell 3.63
Grimsley 3.478
Solomon 3.808
Lehninger 3.77
Nozaki 3.935
DTASelect 4.228
Thurlkill 3.643
EMBOSS 3.795
Sillero 3.935
Patrickios 1.1
IPC_peptide 3.808
IPC2_peptide 3.91
IPC2.peptide.svr19 3.822
Protein with the highest isoelectric point:
>tr|A0A0B8Q2P5|A0A0B8Q2P5_9VIBR Cysteine synthase OS=Vibrio sp. JCM 19236 OX=1481926 GN=JCM19236_5357 PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.49 RR12 pKa = 11.84 KK13 pKa = 7.97 RR14 pKa = 11.84 THH16 pKa = 5.89 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.44 NGRR28 pKa = 11.84 KK29 pKa = 9.39 VINARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.22 GRR39 pKa = 11.84 ARR41 pKa = 11.84 LSKK44 pKa = 10.83
Molecular weight: 5.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.213
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.705
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.969
DTASelect 12.954
Thurlkill 12.969
EMBOSS 13.466
Sillero 12.969
Patrickios 12.427
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.142
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6630
0
6630
1336432
37
1917
201.6
22.39
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.414 ± 0.036
1.014 ± 0.011
5.551 ± 0.036
6.537 ± 0.035
4.237 ± 0.027
6.936 ± 0.033
2.132 ± 0.018
6.238 ± 0.027
5.337 ± 0.029
10.251 ± 0.043
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.835 ± 0.019
4.184 ± 0.02
3.84 ± 0.021
4.313 ± 0.026
4.352 ± 0.028
6.819 ± 0.031
5.488 ± 0.033
7.168 ± 0.03
1.269 ± 0.013
3.088 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here