Lachnospiraceae bacterium NE2001

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; unclassified Lachnospiraceae

Average proteome isoelectric point is 5.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3028 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H9ITX5|A0A1H9ITX5_9FIRM Uncharacterized protein OS=Lachnospiraceae bacterium NE2001 OX=1520823 GN=SAMN02910369_02409 PE=4 SV=1
MM1 pKa = 7.71KK2 pKa = 10.11FRR4 pKa = 11.84RR5 pKa = 11.84LSAVLVAVMILSLVSGCGKK24 pKa = 10.05KK25 pKa = 9.92EE26 pKa = 3.52KK27 pKa = 10.57SAIDD31 pKa = 3.69EE32 pKa = 4.33MKK34 pKa = 10.72SNYY37 pKa = 9.07IKK39 pKa = 10.93YY40 pKa = 8.37VTLGAYY46 pKa = 9.5KK47 pKa = 10.35GVEE50 pKa = 3.91YY51 pKa = 10.25TPAHH55 pKa = 5.71TEE57 pKa = 3.48ITDD60 pKa = 4.66DD61 pKa = 4.14YY62 pKa = 10.82IQYY65 pKa = 10.74DD66 pKa = 3.67IDD68 pKa = 4.25NLISQNTTEE77 pKa = 4.0NQIMEE82 pKa = 5.49GIATMGDD89 pKa = 3.19AVNIDD94 pKa = 3.47FVGYY98 pKa = 10.02IDD100 pKa = 5.84GEE102 pKa = 4.54TFDD105 pKa = 5.26GGDD108 pKa = 3.52SKK110 pKa = 11.65GAGYY114 pKa = 10.68EE115 pKa = 3.96LTLGSGSFIDD125 pKa = 5.01DD126 pKa = 4.73FEE128 pKa = 4.63DD129 pKa = 3.75QICGHH134 pKa = 6.06SPGDD138 pKa = 3.6AFDD141 pKa = 4.35VEE143 pKa = 4.96VTFPDD148 pKa = 4.1DD149 pKa = 3.97YY150 pKa = 11.78GNEE153 pKa = 3.96DD154 pKa = 4.01LAGKK158 pKa = 9.89DD159 pKa = 3.38AVFEE163 pKa = 4.32TTLNYY168 pKa = 9.8IIEE171 pKa = 4.42KK172 pKa = 10.44VEE174 pKa = 3.86PEE176 pKa = 4.12YY177 pKa = 11.51NDD179 pKa = 4.04ALVASATDD187 pKa = 3.57YY188 pKa = 9.6ATTDD192 pKa = 3.12EE193 pKa = 4.93FEE195 pKa = 4.14TAKK198 pKa = 10.7RR199 pKa = 11.84EE200 pKa = 4.2AYY202 pKa = 8.0EE203 pKa = 4.08AQAAEE208 pKa = 4.27SDD210 pKa = 3.9LANDD214 pKa = 3.55KK215 pKa = 10.03STVFNKK221 pKa = 9.98VIEE224 pKa = 4.74DD225 pKa = 3.94STVSEE230 pKa = 4.43YY231 pKa = 10.59PEE233 pKa = 4.75SEE235 pKa = 4.19VNDD238 pKa = 4.98RR239 pKa = 11.84IQMVMDD245 pKa = 4.25SVQQEE250 pKa = 4.2AEE252 pKa = 4.17ANGVDD257 pKa = 3.12IDD259 pKa = 4.71TYY261 pKa = 10.88LSNYY265 pKa = 9.92GYY267 pKa = 10.94DD268 pKa = 3.67LDD270 pKa = 4.77SFKK273 pKa = 11.62DD274 pKa = 3.63NIKK277 pKa = 9.95TSVEE281 pKa = 3.9TYY283 pKa = 9.76IRR285 pKa = 11.84EE286 pKa = 3.95KK287 pKa = 10.16MIVVAIADD295 pKa = 3.72AEE297 pKa = 4.88GITVTDD303 pKa = 3.62EE304 pKa = 4.42EE305 pKa = 4.64VDD307 pKa = 3.57QKK309 pKa = 11.27VQEE312 pKa = 4.31LLEE315 pKa = 4.26QTGLTDD321 pKa = 5.75KK322 pKa = 9.38EE323 pKa = 4.67TLSQQYY329 pKa = 9.81GFKK332 pKa = 10.96DD333 pKa = 2.85EE334 pKa = 4.58DD335 pKa = 4.0YY336 pKa = 9.79YY337 pKa = 11.98YY338 pKa = 10.76EE339 pKa = 3.99VLYY342 pKa = 11.09SKK344 pKa = 10.29IYY346 pKa = 10.7DD347 pKa = 4.56FIYY350 pKa = 9.91EE351 pKa = 3.89NAVAVEE357 pKa = 4.25ASNTDD362 pKa = 3.29ADD364 pKa = 4.24EE365 pKa = 4.77DD366 pKa = 4.49ASDD369 pKa = 4.35SDD371 pKa = 4.86GLVDD375 pKa = 5.77DD376 pKa = 4.8YY377 pKa = 11.64GTTEE381 pKa = 3.94EE382 pKa = 4.41EE383 pKa = 4.17

Molecular weight: 42.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H8YSW5|A0A1H8YSW5_9FIRM Transglutaminase-like superfamily protein OS=Lachnospiraceae bacterium NE2001 OX=1520823 GN=SAMN02910369_00106 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 8.95KK9 pKa = 7.58RR10 pKa = 11.84QRR12 pKa = 11.84SKK14 pKa = 9.07VHH16 pKa = 5.95GFRR19 pKa = 11.84KK20 pKa = 10.01RR21 pKa = 11.84MSTSNGRR28 pKa = 11.84KK29 pKa = 8.74VLSARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.03GRR39 pKa = 11.84KK40 pKa = 8.97SLTVV44 pKa = 3.12

Molecular weight: 5.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3028

0

3028

1117602

40

3668

369.1

41.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.799 ± 0.045

1.193 ± 0.017

6.91 ± 0.035

7.688 ± 0.051

4.177 ± 0.03

6.774 ± 0.038

1.458 ± 0.017

7.793 ± 0.044

7.233 ± 0.054

8.328 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.99 ± 0.027

4.906 ± 0.042

3.051 ± 0.027

2.541 ± 0.025

4.045 ± 0.034

6.413 ± 0.04

5.506 ± 0.053

6.757 ± 0.034

0.856 ± 0.018

4.583 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski