Amino acid dipepetide frequency for Lachnospiraceae bacterium NE2001

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	5.717AlaAla: 5.717 ± 0.116	0.793AlaCys: 0.793 ± 0.029	4.7AlaAsp: 4.7 ± 0.08	5.531AlaGlu: 5.531 ± 0.096	3.026AlaPhe: 3.026 ± 0.057	5.092AlaGly: 5.092 ± 0.085	0.88AlaHis: 0.88 ± 0.028	5.047AlaIle: 5.047 ± 0.07	4.696AlaLys: 4.696 ± 0.083	5.854AlaLeu: 5.854 ± 0.084	1.952AlaMet: 1.952 ± 0.05	2.581AlaAsn: 2.581 ± 0.053	1.973AlaPro: 1.973 ± 0.052	1.62AlaGln: 1.62 ± 0.046	2.697AlaArg: 2.697 ± 0.051	4.183AlaSer: 4.183 ± 0.073	3.408AlaThr: 3.408 ± 0.07	5.064AlaVal: 5.064 ± 0.086	0.534AlaTrp: 0.534 ± 0.023	2.645AlaTyr: 2.645 ± 0.052	0.0AlaXaa: 0.0 ± 0.0
Cys	0.692CysAla: 0.692 ± 0.028	0.186CysCys: 0.186 ± 0.014	0.803CysAsp: 0.803 ± 0.028	0.764CysGlu: 0.764 ± 0.024	0.572CysPhe: 0.572 ± 0.023	1.166CysGly: 1.166 ± 0.034	0.206CysHis: 0.206 ± 0.01	0.957CysIle: 0.957 ± 0.034	0.737CysLys: 0.737 ± 0.03	0.959CysLeu: 0.959 ± 0.03	0.329CysMet: 0.329 ± 0.018	0.525CysAsn: 0.525 ± 0.024	0.465CysPro: 0.465 ± 0.022	0.285CysGln: 0.285 ± 0.015	0.491CysArg: 0.491 ± 0.019	0.768CysSer: 0.768 ± 0.03	0.602CysThr: 0.602 ± 0.023	0.77CysVal: 0.77 ± 0.025	0.11CysTrp: 0.11 ± 0.011	0.544CysTyr: 0.544 ± 0.023	0.0CysXaa: 0.0 ± 0.0
Asp	4.545AspAla: 4.545 ± 0.069	0.621AspCys: 0.621 ± 0.025	4.891AspAsp: 4.891 ± 0.09	6.135AspGlu: 6.135 ± 0.076	3.081AspPhe: 3.081 ± 0.058	4.9AspGly: 4.9 ± 0.09	0.865AspHis: 0.865 ± 0.03	6.045AspIle: 6.045 ± 0.08	5.29AspLys: 5.29 ± 0.092	4.537AspLeu: 4.537 ± 0.072	2.251AspMet: 2.251 ± 0.044	3.328AspAsn: 3.328 ± 0.06	1.893AspPro: 1.893 ± 0.038	1.283AspGln: 1.283 ± 0.037	2.667AspArg: 2.667 ± 0.054	4.399AspSer: 4.399 ± 0.076	3.635AspThr: 3.635 ± 0.075	4.367AspVal: 4.367 ± 0.066	0.6AspTrp: 0.6 ± 0.03	3.763AspTyr: 3.763 ± 0.07	0.0AspXaa: 0.0 ± 0.0
Glu	5.402GluAla: 5.402 ± 0.1	0.743GluCys: 0.743 ± 0.024	5.099GluAsp: 5.099 ± 0.079	7.076GluGlu: 7.076 ± 0.122	2.767GluPhe: 2.767 ± 0.054	4.501GluGly: 4.501 ± 0.061	1.112GluHis: 1.112 ± 0.035	5.577GluIle: 5.577 ± 0.074	6.764GluLys: 6.764 ± 0.112	6.744GluLeu: 6.744 ± 0.093	2.598GluMet: 2.598 ± 0.057	4.3GluAsn: 4.3 ± 0.062	1.926GluPro: 1.926 ± 0.077	2.176GluGln: 2.176 ± 0.045	3.163GluArg: 3.163 ± 0.062	4.04GluSer: 4.04 ± 0.066	3.99GluThr: 3.99 ± 0.072	4.456GluVal: 4.456 ± 0.069	0.671GluTrp: 0.671 ± 0.029	3.779GluTyr: 3.779 ± 0.073	0.0GluXaa: 0.0 ± 0.0
Phe	2.746PheAla: 2.746 ± 0.051	0.619PheCys: 0.619 ± 0.023	3.221PheAsp: 3.221 ± 0.055	2.871PheGlu: 2.871 ± 0.051	1.904PhePhe: 1.904 ± 0.052	3.022PheGly: 3.022 ± 0.055	0.657PheHis: 0.657 ± 0.023	3.341PheIle: 3.341 ± 0.073	2.53PheLys: 2.53 ± 0.045	3.424PheLeu: 3.424 ± 0.064	1.229PheMet: 1.229 ± 0.037	1.909PheAsn: 1.909 ± 0.04	1.214PhePro: 1.214 ± 0.038	0.943PheGln: 0.943 ± 0.03	1.564PheArg: 1.564 ± 0.039	2.9PheSer: 2.9 ± 0.049	2.402PheThr: 2.402 ± 0.055	3.059PheVal: 3.059 ± 0.064	0.387PheTrp: 0.387 ± 0.018	1.829PheTyr: 1.829 ± 0.046	0.0PheXaa: 0.0 ± 0.0
Gly	4.426GlyAla: 4.426 ± 0.082	0.985GlyCys: 0.985 ± 0.029	4.246GlyAsp: 4.246 ± 0.065	4.579GlyGlu: 4.579 ± 0.079	3.191GlyPhe: 3.191 ± 0.06	4.546GlyGly: 4.546 ± 0.087	1.073GlyHis: 1.073 ± 0.032	5.704GlyIle: 5.704 ± 0.077	4.975GlyLys: 4.975 ± 0.069	5.608GlyLeu: 5.608 ± 0.088	2.146GlyMet: 2.146 ± 0.058	3.214GlyAsn: 3.214 ± 0.071	1.282GlyPro: 1.282 ± 0.04	1.763GlyGln: 1.763 ± 0.062	2.691GlyArg: 2.691 ± 0.056	4.146GlySer: 4.146 ± 0.074	4.036GlyThr: 4.036 ± 0.08	4.857GlyVal: 4.857 ± 0.076	0.8GlyTrp: 0.8 ± 0.039	3.467GlyTyr: 3.467 ± 0.061	0.0GlyXaa: 0.0 ± 0.0
His	0.764HisAla: 0.764 ± 0.028	0.193HisCys: 0.193 ± 0.014	0.893HisAsp: 0.893 ± 0.031	0.873HisGlu: 0.873 ± 0.03	0.767HisPhe: 0.767 ± 0.028	1.058HisGly: 1.058 ± 0.037	0.347HisHis: 0.347 ± 0.034	1.344HisIle: 1.344 ± 0.039	0.901HisLys: 0.901 ± 0.025	1.251HisLeu: 1.251 ± 0.032	0.467HisMet: 0.467 ± 0.022	0.776HisAsn: 0.776 ± 0.027	0.667HisPro: 0.667 ± 0.024	0.358HisGln: 0.358 ± 0.019	0.572HisArg: 0.572 ± 0.024	0.958HisSer: 0.958 ± 0.031	0.814HisThr: 0.814 ± 0.028	0.819HisVal: 0.819 ± 0.031	0.122HisTrp: 0.122 ± 0.011	0.634HisTyr: 0.634 ± 0.023	0.0HisXaa: 0.0 ± 0.0
Ile	5.336IleAla: 5.336 ± 0.082	1.183IleCys: 1.183 ± 0.038	5.276IleAsp: 5.276 ± 0.07	5.418IleGlu: 5.418 ± 0.082	3.171IlePhe: 3.171 ± 0.071	4.715IleGly: 4.715 ± 0.073	1.145IleHis: 1.145 ± 0.032	6.416IleIle: 6.416 ± 0.108	5.268IleLys: 5.268 ± 0.072	6.56IleLeu: 6.56 ± 0.102	2.011IleMet: 2.011 ± 0.05	3.922IleAsn: 3.922 ± 0.075	2.98IlePro: 2.98 ± 0.051	1.877IleGln: 1.877 ± 0.044	3.244IleArg: 3.244 ± 0.061	5.717IleSer: 5.717 ± 0.084	4.498IleThr: 4.498 ± 0.074	5.336IleVal: 5.336 ± 0.078	0.565IleTrp: 0.565 ± 0.024	3.287IleTyr: 3.287 ± 0.063	0.0IleXaa: 0.0 ± 0.0
Lys	5.046LysAla: 5.046 ± 0.087	0.748LysCys: 0.748 ± 0.031	5.106LysAsp: 5.106 ± 0.089	6.408LysGlu: 6.408 ± 0.101	1.952LysPhe: 1.952 ± 0.047	4.073LysGly: 4.073 ± 0.056	0.999LysHis: 0.999 ± 0.032	4.763LysIle: 4.763 ± 0.066	6.87LysLys: 6.87 ± 0.123	6.087LysLeu: 6.087 ± 0.081	2.138LysMet: 2.138 ± 0.05	4.178LysAsn: 4.178 ± 0.069	2.038LysPro: 2.038 ± 0.05	2.004LysGln: 2.004 ± 0.052	3.043LysArg: 3.043 ± 0.057	4.093LysSer: 4.093 ± 0.068	4.232LysThr: 4.232 ± 0.075	4.461LysVal: 4.461 ± 0.07	0.678LysTrp: 0.678 ± 0.028	3.417LysTyr: 3.417 ± 0.057	0.0LysXaa: 0.0 ± 0.0
Leu	5.9LeuAla: 5.9 ± 0.094	1.107LeuCys: 1.107 ± 0.037	5.614LeuAsp: 5.614 ± 0.077	5.808LeuGlu: 5.808 ± 0.08	3.516LeuPhe: 3.516 ± 0.066	5.606LeuGly: 5.606 ± 0.081	1.207LeuHis: 1.207 ± 0.035	6.216LeuIle: 6.216 ± 0.097	5.839LeuLys: 5.839 ± 0.08	7.213LeuLeu: 7.213 ± 0.111	2.469LeuMet: 2.469 ± 0.047	3.83LeuAsn: 3.83 ± 0.056	2.8LeuPro: 2.8 ± 0.051	1.988LeuGln: 1.988 ± 0.044	3.491LeuArg: 3.491 ± 0.068	6.396LeuSer: 6.396 ± 0.097	4.701LeuThr: 4.701 ± 0.074	5.482LeuVal: 5.482 ± 0.082	0.619LeuTrp: 0.619 ± 0.023	3.479LeuTyr: 3.479 ± 0.061	0.0LeuXaa: 0.0 ± 0.0
Met	2.139MetAla: 2.139 ± 0.049	0.356MetCys: 0.356 ± 0.017	2.032MetAsp: 2.032 ± 0.043	1.984MetGlu: 1.984 ± 0.042	1.025MetPhe: 1.025 ± 0.035	1.989MetGly: 1.989 ± 0.052	0.378MetHis: 0.378 ± 0.019	2.281MetIle: 2.281 ± 0.053	2.45MetLys: 2.45 ± 0.049	2.539MetLeu: 2.539 ± 0.056	1.068MetMet: 1.068 ± 0.033	1.734MetAsn: 1.734 ± 0.047	1.021MetPro: 1.021 ± 0.037	0.787MetGln: 0.787 ± 0.032	1.194MetArg: 1.194 ± 0.037	2.077MetSer: 2.077 ± 0.046	1.666MetThr: 1.666 ± 0.038	1.883MetVal: 1.883 ± 0.041	0.215MetTrp: 0.215 ± 0.015	1.081MetTyr: 1.081 ± 0.029	0.0MetXaa: 0.0 ± 0.0
Asn	3.252AsnAla: 3.252 ± 0.07	0.578AsnCys: 0.578 ± 0.02	3.167AsnAsp: 3.167 ± 0.057	3.567AsnGlu: 3.567 ± 0.068	1.554AsnPhe: 1.554 ± 0.04	3.552AsnGly: 3.552 ± 0.069	0.746AsnHis: 0.746 ± 0.029	4.043AsnIle: 4.043 ± 0.072	3.433AsnLys: 3.433 ± 0.07	3.862AsnLeu: 3.862 ± 0.063	1.56AsnMet: 1.56 ± 0.041	2.658AsnAsn: 2.658 ± 0.095	2.075AsnPro: 2.075 ± 0.056	1.431AsnGln: 1.431 ± 0.051	1.96AsnArg: 1.96 ± 0.046	2.933AsnSer: 2.933 ± 0.058	2.56AsnThr: 2.56 ± 0.048	3.427AsnVal: 3.427 ± 0.061	0.428AsnTrp: 0.428 ± 0.02	2.279AsnTyr: 2.279 ± 0.052	0.0AsnXaa: 0.0 ± 0.0
Pro	2.038ProAla: 2.038 ± 0.051	0.297ProCys: 0.297 ± 0.015	2.652ProAsp: 2.652 ± 0.053	3.304ProGlu: 3.304 ± 0.079	1.312ProPhe: 1.312 ± 0.033	2.044ProGly: 2.044 ± 0.046	0.45ProHis: 0.45 ± 0.019	2.173ProIle: 2.173 ± 0.047	1.824ProLys: 1.824 ± 0.042	2.377ProLeu: 2.377 ± 0.045	0.825ProMet: 0.825 ± 0.033	1.278ProAsn: 1.278 ± 0.037	0.618ProPro: 0.618 ± 0.025	0.908ProGln: 0.908 ± 0.046	0.921ProArg: 0.921 ± 0.029	1.753ProSer: 1.753 ± 0.043	1.539ProThr: 1.539 ± 0.041	2.515ProVal: 2.515 ± 0.048	0.254ProTrp: 0.254 ± 0.017	1.427ProTyr: 1.427 ± 0.042	0.0ProXaa: 0.0 ± 0.0
Gln	1.741GlnAla: 1.741 ± 0.047	0.287GlnCys: 0.287 ± 0.018	1.534GlnAsp: 1.534 ± 0.037	1.858GlnGlu: 1.858 ± 0.049	1.0GlnPhe: 1.0 ± 0.029	1.609GlnGly: 1.609 ± 0.054	0.389GlnHis: 0.389 ± 0.019	1.865GlnIle: 1.865 ± 0.044	1.951GlnLys: 1.951 ± 0.057	2.182GlnLeu: 2.182 ± 0.045	0.96GlnMet: 0.96 ± 0.032	1.406GlnAsn: 1.406 ± 0.046	0.828GlnPro: 0.828 ± 0.046	0.991GlnGln: 0.991 ± 0.062	1.135GlnArg: 1.135 ± 0.036	1.46GlnSer: 1.46 ± 0.039	1.292GlnThr: 1.292 ± 0.04	1.538GlnVal: 1.538 ± 0.04	0.216GlnTrp: 0.216 ± 0.017	1.166GlnTyr: 1.166 ± 0.041	0.0GlnXaa: 0.0 ± 0.0
Arg	2.394ArgAla: 2.394 ± 0.045	0.477ArgCys: 0.477 ± 0.021	2.717ArgAsp: 2.717 ± 0.053	3.351ArgGlu: 3.351 ± 0.063	1.927ArgPhe: 1.927 ± 0.041	2.289ArgGly: 2.289 ± 0.05	0.673ArgHis: 0.673 ± 0.028	3.367ArgIle: 3.367 ± 0.063	2.992ArgLys: 2.992 ± 0.052	3.641ArgLeu: 3.641 ± 0.08	1.28ArgMet: 1.28 ± 0.036	1.964ArgAsn: 1.964 ± 0.045	1.146ArgPro: 1.146 ± 0.031	1.155ArgGln: 1.155 ± 0.033	2.126ArgArg: 2.126 ± 0.052	2.16ArgSer: 2.16 ± 0.046	2.011ArgThr: 2.011 ± 0.043	2.615ArgVal: 2.615 ± 0.052	0.32ArgTrp: 0.32 ± 0.019	1.845ArgTyr: 1.845 ± 0.045	0.0ArgXaa: 0.0 ± 0.0
Ser	3.945SerAla: 3.945 ± 0.074	0.655SerCys: 0.655 ± 0.029	4.72SerAsp: 4.72 ± 0.078	4.758SerGlu: 4.758 ± 0.08	3.127SerPhe: 3.127 ± 0.054	5.234SerGly: 5.234 ± 0.087	0.897SerHis: 0.897 ± 0.032	4.843SerIle: 4.843 ± 0.087	4.164SerLys: 4.164 ± 0.07	5.421SerLeu: 5.421 ± 0.08	1.696SerMet: 1.696 ± 0.039	3.023SerAsn: 3.023 ± 0.074	1.739SerPro: 1.739 ± 0.04	1.632SerGln: 1.632 ± 0.04	2.636SerArg: 2.636 ± 0.057	4.455SerSer: 4.455 ± 0.083	3.278SerThr: 3.278 ± 0.072	4.454SerVal: 4.454 ± 0.069	0.537SerTrp: 0.537 ± 0.026	2.912SerTyr: 2.912 ± 0.056	0.0SerXaa: 0.0 ± 0.0
Thr	3.959ThrAla: 3.959 ± 0.076	0.513ThrCys: 0.513 ± 0.022	3.908ThrAsp: 3.908 ± 0.074	4.246ThrGlu: 4.246 ± 0.088	2.476ThrPhe: 2.476 ± 0.049	4.421ThrGly: 4.421 ± 0.082	0.805ThrHis: 0.805 ± 0.031	4.183ThrIle: 4.183 ± 0.075	3.332ThrLys: 3.332 ± 0.064	4.57ThrLeu: 4.57 ± 0.074	1.314ThrMet: 1.314 ± 0.035	2.35ThrAsn: 2.35 ± 0.05	2.023ThrPro: 2.023 ± 0.044	1.203ThrGln: 1.203 ± 0.036	1.957ThrArg: 1.957 ± 0.046	3.38ThrSer: 3.38 ± 0.07	3.212ThrThr: 3.212 ± 0.095	4.235ThrVal: 4.235 ± 0.091	0.442ThrTrp: 0.442 ± 0.021	2.532ThrTyr: 2.532 ± 0.062	0.0ThrXaa: 0.0 ± 0.0
Val	4.714ValAla: 4.714 ± 0.08	0.947ValCys: 0.947 ± 0.032	4.541ValAsp: 4.541 ± 0.074	4.646ValGlu: 4.646 ± 0.074	2.966ValPhe: 2.966 ± 0.06	4.23ValGly: 4.23 ± 0.071	0.872ValHis: 0.872 ± 0.03	5.456ValIle: 5.456 ± 0.078	4.623ValLys: 4.623 ± 0.071	5.904ValLeu: 5.904 ± 0.09	1.943ValMet: 1.943 ± 0.045	3.189ValAsn: 3.189 ± 0.063	2.213ValPro: 2.213 ± 0.048	1.543ValGln: 1.543 ± 0.037	2.707ValArg: 2.707 ± 0.053	4.749ValSer: 4.749 ± 0.082	4.083ValThr: 4.083 ± 0.086	4.863ValVal: 4.863 ± 0.088	0.568ValTrp: 0.568 ± 0.024	2.811ValTyr: 2.811 ± 0.05	0.0ValXaa: 0.0 ± 0.0
Trp	0.527TrpAla: 0.527 ± 0.025	0.132TrpCys: 0.132 ± 0.011	0.591TrpAsp: 0.591 ± 0.025	0.503TrpGlu: 0.503 ± 0.025	0.408TrpPhe: 0.408 ± 0.022	0.589TrpGly: 0.589 ± 0.026	0.161TrpHis: 0.161 ± 0.011	0.64TrpIle: 0.64 ± 0.027	0.568TrpLys: 0.568 ± 0.024	0.787TrpLeu: 0.787 ± 0.028	0.301TrpMet: 0.301 ± 0.017	0.497TrpAsn: 0.497 ± 0.024	0.173TrpPro: 0.173 ± 0.013	0.253TrpGln: 0.253 ± 0.015	0.303TrpArg: 0.303 ± 0.017	0.489TrpSer: 0.489 ± 0.023	0.431TrpThr: 0.431 ± 0.024	0.523TrpVal: 0.523 ± 0.024	0.122TrpTrp: 0.122 ± 0.014	0.559TrpTyr: 0.559 ± 0.038	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.712TyrAla: 2.712 ± 0.048	0.513TyrCys: 0.513 ± 0.023	3.387TyrAsp: 3.387 ± 0.061	3.203TyrGlu: 3.203 ± 0.059	2.03TyrPhe: 2.03 ± 0.042	3.1TyrGly: 3.1 ± 0.063	0.714TyrHis: 0.714 ± 0.023	3.669TyrIle: 3.669 ± 0.071	3.125TyrLys: 3.125 ± 0.06	3.761TyrLeu: 3.761 ± 0.054	1.362TyrMet: 1.362 ± 0.042	2.403TyrAsn: 2.403 ± 0.058	1.433TyrPro: 1.433 ± 0.039	1.206TyrGln: 1.206 ± 0.035	1.887TyrArg: 1.887 ± 0.043	3.072TyrSer: 3.072 ± 0.067	2.67TyrThr: 2.67 ± 0.07	2.842TyrVal: 2.842 ± 0.06	0.368TyrTrp: 0.368 ± 0.022	2.37TyrTyr: 2.37 ± 0.06	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3028 proteins (1117602 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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