Sweet potato collusive virus
Average proteome isoelectric point is 6.6
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|F2XXY6|F2XXY6_9VIRU Uncharacterized protein OS=Sweet potato collusive virus OX=930168 PE=4 SV=1
MM1 pKa = 7.63 EE2 pKa = 6.09 KK3 pKa = 9.58 LTSLLISCPEE13 pKa = 4.13 MEE15 pKa = 5.63 QMMQQLLAEE24 pKa = 4.24 MQKK27 pKa = 11.14 LNATVEE33 pKa = 4.4 KK34 pKa = 9.97 QNSRR38 pKa = 11.84 IEE40 pKa = 3.94 QLEE43 pKa = 4.06 NEE45 pKa = 4.33 LEE47 pKa = 4.11 KK48 pKa = 11.24 EE49 pKa = 4.07 KK50 pKa = 10.96 GKK52 pKa = 11.02 KK53 pKa = 9.43 PLTEE57 pKa = 3.76 EE58 pKa = 4.39 KK59 pKa = 10.25 IISEE63 pKa = 4.21 KK64 pKa = 10.56 EE65 pKa = 3.76 LEE67 pKa = 4.15 KK68 pKa = 10.91 EE69 pKa = 5.34 KK70 pKa = 10.74 EE71 pKa = 4.07 ISWKK75 pKa = 10.88 AKK77 pKa = 7.42 IKK79 pKa = 10.54 KK80 pKa = 9.85 YY81 pKa = 11.03 GEE83 pKa = 4.14 ASSKK87 pKa = 9.13 EE88 pKa = 3.82 EE89 pKa = 4.16 KK90 pKa = 10.14 IIEE93 pKa = 4.06 KK94 pKa = 10.55 DD95 pKa = 3.04 VWQKK99 pKa = 11.21 PKK101 pKa = 9.27 GTHH104 pKa = 5.61 IGSIYY109 pKa = 8.26 VTEE112 pKa = 5.2 FVRR115 pKa = 11.84 LMNYY119 pKa = 10.09 LNSRR123 pKa = 11.84 HH124 pKa = 6.25 ANLNEE129 pKa = 3.96 IYY131 pKa = 10.83 SLTDD135 pKa = 3.29 YY136 pKa = 11.37 NKK138 pKa = 10.6 LVADD142 pKa = 4.01 KK143 pKa = 10.03 NTDD146 pKa = 2.97 EE147 pKa = 4.2 RR148 pKa = 11.84 LIRR151 pKa = 11.84 AAYY154 pKa = 8.85 QHH156 pKa = 6.46 GLLHH160 pKa = 4.79 VHH162 pKa = 6.58 YY163 pKa = 9.9 IEE165 pKa = 4.93 SANQIKK171 pKa = 9.78 MYY173 pKa = 10.87 DD174 pKa = 3.45 EE175 pKa = 5.58 GIVNAYY181 pKa = 10.19 LKK183 pKa = 10.57 LAQLTKK189 pKa = 10.66 AKK191 pKa = 10.0 CIYY194 pKa = 9.99 IRR196 pKa = 11.84 FYY198 pKa = 10.47 TAFAEE203 pKa = 4.55 VTKK206 pKa = 10.67 EE207 pKa = 3.83 GIIPKK212 pKa = 9.86 IEE214 pKa = 4.5 AIKK217 pKa = 10.82 LGITYY222 pKa = 9.79 EE223 pKa = 4.17 KK224 pKa = 10.09 IQYY227 pKa = 8.71 DD228 pKa = 3.66 VCEE231 pKa = 4.03 QEE233 pKa = 4.93 LFEE236 pKa = 4.82 EE237 pKa = 4.34 NRR239 pKa = 11.84 VHH241 pKa = 7.89 DD242 pKa = 4.36 FLRR245 pKa = 11.84 HH246 pKa = 5.06 KK247 pKa = 10.43 KK248 pKa = 10.53 AIGLLTIRR256 pKa = 11.84 RR257 pKa = 11.84 EE258 pKa = 4.22 LEE260 pKa = 3.95 STEE263 pKa = 3.93 GNIWVYY269 pKa = 8.98 EE270 pKa = 3.9 NRR272 pKa = 11.84 NNRR275 pKa = 11.84 IIYY278 pKa = 9.81 APSRR282 pKa = 11.84 ARR284 pKa = 11.84 KK285 pKa = 9.0 EE286 pKa = 3.97 EE287 pKa = 3.95 VKK289 pKa = 10.66 KK290 pKa = 11.08 VVAAWQQKK298 pKa = 9.39 IYY300 pKa = 9.98 TPEE303 pKa = 4.02 RR304 pKa = 11.84 NIPDD308 pKa = 3.82 CGITAPCISEE318 pKa = 4.07 PCLKK322 pKa = 10.13 ILCEE326 pKa = 3.91 LSKK329 pKa = 11.02 NQLVVKK335 pKa = 8.42 HH336 pKa = 4.65 QCKK339 pKa = 10.12 YY340 pKa = 8.58 CGKK343 pKa = 8.35 MKK345 pKa = 10.48 KK346 pKa = 9.67 KK347 pKa = 10.83 EE348 pKa = 4.04 EE349 pKa = 3.99 VDD351 pKa = 3.37 YY352 pKa = 11.28 SLPEE356 pKa = 4.6 DD357 pKa = 4.46 IIMKK361 pKa = 10.43 DD362 pKa = 3.11 VDD364 pKa = 3.83 EE365 pKa = 5.65 DD366 pKa = 4.64 SDD368 pKa = 4.66 VEE370 pKa = 4.57 DD371 pKa = 3.9 KK372 pKa = 11.35 KK373 pKa = 11.52 EE374 pKa = 3.64 EE375 pKa = 3.83 NAYY378 pKa = 10.74 DD379 pKa = 3.75 KK380 pKa = 11.42 DD381 pKa = 3.34 AFRR384 pKa = 11.84 PDD386 pKa = 3.17 KK387 pKa = 11.19 VADD390 pKa = 4.05 AGDD393 pKa = 3.89 DD394 pKa = 3.8 VKK396 pKa = 11.43 EE397 pKa = 4.15 KK398 pKa = 11.11
Molecular weight: 46.46 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.729
IPC2_protein 5.83
IPC_protein 5.83
Toseland 6.046
ProMoST 6.122
Dawson 6.02
Bjellqvist 6.059
Wikipedia 5.982
Rodwell 5.995
Grimsley 6.135
Solomon 6.02
Lehninger 5.995
Nozaki 6.249
DTASelect 6.415
Thurlkill 6.389
EMBOSS 6.351
Sillero 6.351
Patrickios 4.279
IPC_peptide 6.033
IPC2_peptide 6.275
IPC2.peptide.svr19 6.419
Protein with the highest isoelectric point:
>tr|F2XXY4|F2XXY4_9VIRU Coat protein/movement protein OS=Sweet potato collusive virus OX=930168 PE=4 SV=1
MM1 pKa = 7.51 NRR3 pKa = 11.84 MTYY6 pKa = 7.35 MTVKK10 pKa = 9.29 ITIPTYY16 pKa = 9.17 MSRR19 pKa = 11.84 IYY21 pKa = 10.52 HH22 pKa = 5.97 GLFDD26 pKa = 4.17 TGANTCVCKK35 pKa = 10.31 TKK37 pKa = 10.62 VLPPEE42 pKa = 3.61 KK43 pKa = 9.59 WIEE46 pKa = 3.8 IDD48 pKa = 3.66 NKK50 pKa = 10.13 VLTGFSEE57 pKa = 4.24 EE58 pKa = 4.28 KK59 pKa = 10.79 NVIKK63 pKa = 10.85 YY64 pKa = 10.22 RR65 pKa = 11.84 ADD67 pKa = 3.51 NIKK70 pKa = 10.51 IMIAKK75 pKa = 9.67 KK76 pKa = 10.33 EE77 pKa = 4.0 FIIPYY82 pKa = 9.06 IYY84 pKa = 11.31 AMDD87 pKa = 5.36 NINADD92 pKa = 3.87 IIIGATFYY100 pKa = 11.36 NKK102 pKa = 10.1 YY103 pKa = 10.71 SPITIDD109 pKa = 3.38 MEE111 pKa = 4.04 KK112 pKa = 10.84 GIIKK116 pKa = 8.07 FTNKK120 pKa = 10.29 GEE122 pKa = 4.17 VYY124 pKa = 10.25 PNYY127 pKa = 9.91 LVKK130 pKa = 10.77 YY131 pKa = 8.17 PRR133 pKa = 11.84 KK134 pKa = 9.44 KK135 pKa = 10.86 VLIPWEE141 pKa = 4.4 KK142 pKa = 10.45 GNPSIIQPLQNEE154 pKa = 4.42 QINNINEE161 pKa = 3.97 VEE163 pKa = 4.16 EE164 pKa = 4.25 LNNILGEE171 pKa = 4.77 DD172 pKa = 3.47 IYY174 pKa = 11.56 GDD176 pKa = 3.51 NPLKK180 pKa = 10.49 HH181 pKa = 5.8 WEE183 pKa = 3.97 KK184 pKa = 10.45 HH185 pKa = 3.27 KK186 pKa = 9.8 TYY188 pKa = 11.25 AKK190 pKa = 10.18 IEE192 pKa = 4.21 LKK194 pKa = 11.05 NPDD197 pKa = 3.25 DD198 pKa = 5.46 HH199 pKa = 8.4 IYY201 pKa = 10.46 KK202 pKa = 10.44 PPINYY207 pKa = 9.16 QEE209 pKa = 3.94 SDD211 pKa = 3.52 YY212 pKa = 11.62 KK213 pKa = 9.92 EE214 pKa = 4.26 FKK216 pKa = 9.41 MHH218 pKa = 6.44 IDD220 pKa = 3.46 EE221 pKa = 4.26 MVKK224 pKa = 10.17 EE225 pKa = 4.36 GFIEE229 pKa = 4.16 EE230 pKa = 4.54 CKK232 pKa = 10.64 NLEE235 pKa = 3.98 NKK237 pKa = 10.09 KK238 pKa = 9.7 YY239 pKa = 10.14 SSPAFIVNKK248 pKa = 9.82 HH249 pKa = 5.02 SEE251 pKa = 3.94 IKK253 pKa = 9.97 RR254 pKa = 11.84 GKK256 pKa = 8.92 SRR258 pKa = 11.84 MVIDD262 pKa = 4.34 YY263 pKa = 10.98 KK264 pKa = 11.3 DD265 pKa = 3.65 LNKK268 pKa = 9.74 KK269 pKa = 10.44 AKK271 pKa = 9.72 VIKK274 pKa = 10.48 HH275 pKa = 6.75 PIPNKK280 pKa = 10.33 DD281 pKa = 2.85 ILINRR286 pKa = 11.84 GIKK289 pKa = 10.15 ANYY292 pKa = 8.88 FSKK295 pKa = 10.63 FDD297 pKa = 4.02 CKK299 pKa = 10.9 SGFYY303 pKa = 10.05 HH304 pKa = 7.06 IKK306 pKa = 10.66 LEE308 pKa = 4.19 EE309 pKa = 4.4 DD310 pKa = 3.45 SKK312 pKa = 11.49 KK313 pKa = 9.19 YY314 pKa = 8.84 TAFTVPQGYY323 pKa = 7.93 YY324 pKa = 9.0 VWIVLPFGYY333 pKa = 10.17 HH334 pKa = 6.06 NSPSIYY340 pKa = 9.25 QQFMDD345 pKa = 5.31 GIFRR349 pKa = 11.84 PYY351 pKa = 10.14 YY352 pKa = 10.54 DD353 pKa = 5.22 FILVYY358 pKa = 10.17 IDD360 pKa = 5.59 DD361 pKa = 4.06 ILIFSKK367 pKa = 9.69 TYY369 pKa = 9.84 EE370 pKa = 3.75 EE371 pKa = 4.49 HH372 pKa = 7.19 KK373 pKa = 10.16 IHH375 pKa = 7.8 LEE377 pKa = 3.42 IFRR380 pKa = 11.84 NIIIKK385 pKa = 10.35 HH386 pKa = 5.96 GIVLSKK392 pKa = 10.62 KK393 pKa = 9.07 KK394 pKa = 10.88 AEE396 pKa = 3.95 IGKK399 pKa = 9.38 QKK401 pKa = 10.55 IEE403 pKa = 3.86 FLGVKK408 pKa = 9.49 IEE410 pKa = 3.93 QGGIEE415 pKa = 4.57 LQPHH419 pKa = 7.13 IIDD422 pKa = 5.09 KK423 pKa = 10.69 ILEE426 pKa = 3.87 KK427 pKa = 10.43 HH428 pKa = 6.29 IKK430 pKa = 9.63 IKK432 pKa = 10.51 SKK434 pKa = 11.17 KK435 pKa = 8.31 EE436 pKa = 3.67 LQSILGLVNQIRR448 pKa = 11.84 NFLPNLSKK456 pKa = 10.62 ILLPIQKK463 pKa = 9.83 KK464 pKa = 10.4 LKK466 pKa = 10.11 IKK468 pKa = 10.81 NEE470 pKa = 4.25 EE471 pKa = 3.53 VWEE474 pKa = 4.11 WTKK477 pKa = 11.29 EE478 pKa = 3.89 DD479 pKa = 3.54 EE480 pKa = 4.92 QNIIKK485 pKa = 10.71 LKK487 pKa = 10.42 DD488 pKa = 3.38 YY489 pKa = 11.33 CKK491 pKa = 11.12 DD492 pKa = 3.29 NVIKK496 pKa = 8.4 MTYY499 pKa = 9.14 PVEE502 pKa = 4.18 EE503 pKa = 5.14 KK504 pKa = 11.01 DD505 pKa = 3.58 MNWIIEE511 pKa = 3.89 VDD513 pKa = 3.37 ASKK516 pKa = 10.71 EE517 pKa = 3.99 YY518 pKa = 11.04 YY519 pKa = 10.66 GNCLKK524 pKa = 10.84 YY525 pKa = 10.56 KK526 pKa = 9.6 KK527 pKa = 10.4 DD528 pKa = 3.42 KK529 pKa = 10.8 IEE531 pKa = 4.05 YY532 pKa = 7.44 ICRR535 pKa = 11.84 YY536 pKa = 9.69 NSGTFKK542 pKa = 10.54 EE543 pKa = 4.43 HH544 pKa = 6.35 EE545 pKa = 4.2 KK546 pKa = 11.03 NYY548 pKa = 10.52 DD549 pKa = 3.16 INRR552 pKa = 11.84 KK553 pKa = 7.67 EE554 pKa = 4.49 LIAIYY559 pKa = 10.13 KK560 pKa = 9.78 GLEE563 pKa = 3.72 HH564 pKa = 6.44 YY565 pKa = 10.81 AIFTTQGKK573 pKa = 9.77 KK574 pKa = 9.68 LVRR577 pKa = 11.84 TDD579 pKa = 3.19 NSQAYY584 pKa = 8.67 YY585 pKa = 9.54 WIKK588 pKa = 10.43 NSKK591 pKa = 9.04 IKK593 pKa = 10.92 NSIDD597 pKa = 3.25 MKK599 pKa = 10.15 NVKK602 pKa = 10.25 GILAKK607 pKa = 10.18 IIMYY611 pKa = 10.43 DD612 pKa = 3.58 FDD614 pKa = 4.77 IEE616 pKa = 4.71 IIDD619 pKa = 3.88 GKK621 pKa = 8.73 TNIVADD627 pKa = 4.2 FLSRR631 pKa = 11.84 NGTDD635 pKa = 3.54 DD636 pKa = 3.3 ATITGG641 pKa = 3.86
Molecular weight: 75.5 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.376
IPC2_protein 8.375
IPC_protein 8.2
Toseland 8.916
ProMoST 8.77
Dawson 9.238
Bjellqvist 9.019
Wikipedia 9.399
Rodwell 9.472
Grimsley 9.297
Solomon 9.268
Lehninger 9.224
Nozaki 9.092
DTASelect 8.96
Thurlkill 9.092
EMBOSS 9.385
Sillero 9.224
Patrickios 4.431
IPC_peptide 9.268
IPC2_peptide 7.541
IPC2.peptide.svr19 7.59
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
2352
49
1264
588.0
69.47
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.316 ± 0.986
1.19 ± 0.354
5.57 ± 0.136
10.544 ± 1.23
2.976 ± 0.457
3.614 ± 0.422
1.616 ± 0.435
12.287 ± 1.061
12.67 ± 0.521
6.845 ± 0.475
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.168 ± 0.083
7.526 ± 1.045
3.316 ± 0.248
3.869 ± 0.549
3.699 ± 0.593
4.677 ± 0.401
4.124 ± 0.243
3.444 ± 0.777
0.978 ± 0.144
5.57 ± 0.601
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here