Rhodobacteraceae bacterium TG-679

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; unclassified Rhodobacteraceae

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3860 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2V2LJA1|A0A2V2LJA1_9RHOB Ribosomal protein S12 methylthiotransferase RimO OS=Rhodobacteraceae bacterium TG-679 OX=2072018 GN=rimO PE=3 SV=1
MM1 pKa = 7.2PQVFRR6 pKa = 11.84NACFARR12 pKa = 11.84PNGGIRR18 pKa = 11.84LQLKK22 pKa = 10.18RR23 pKa = 11.84GGPLEE28 pKa = 4.49DD29 pKa = 3.69HH30 pKa = 6.59LKK32 pKa = 10.48SYY34 pKa = 10.26EE35 pKa = 4.05GNHH38 pKa = 5.2MKK40 pKa = 10.49KK41 pKa = 9.79ILLASTALIVSAGFASAEE59 pKa = 4.17VVIGGDD65 pKa = 3.25GYY67 pKa = 10.96MGVAYY72 pKa = 9.85GDD74 pKa = 3.31NGNPIFATTDD84 pKa = 3.04TSADD88 pKa = 3.58QVPAGSGNNFSNYY101 pKa = 10.06SFVYY105 pKa = 10.69DD106 pKa = 4.46LDD108 pKa = 3.8VDD110 pKa = 5.13FKK112 pKa = 11.74ASGTSDD118 pKa = 2.88SGLTFGAAGDD128 pKa = 4.08FDD130 pKa = 6.03DD131 pKa = 6.26LGASQGARR139 pKa = 11.84GWDD142 pKa = 3.13NSIFISGDD150 pKa = 2.94FGTLTMGDD158 pKa = 2.94IDD160 pKa = 3.9GAAEE164 pKa = 3.98NVIGDD169 pKa = 4.11LAGVGLSGLGDD180 pKa = 3.6FNEE183 pKa = 4.42NIFLIGAGAQPAGPVARR200 pKa = 11.84YY201 pKa = 9.76DD202 pKa = 3.71YY203 pKa = 10.78AISGLTLSLGLSDD216 pKa = 5.04DD217 pKa = 3.32SGYY220 pKa = 11.34NIGASYY226 pKa = 8.86ATDD229 pKa = 3.88LFSVGLAYY237 pKa = 10.28EE238 pKa = 4.38DD239 pKa = 3.98VADD242 pKa = 4.44GATVTLFDD250 pKa = 4.29IDD252 pKa = 4.12ALGSTGSTAITATAPNGASQIIGAASVTFANVTLKK287 pKa = 10.81GVYY290 pKa = 9.83GQIDD294 pKa = 3.66VDD296 pKa = 3.87GTTVDD301 pKa = 3.22TFDD304 pKa = 3.35QYY306 pKa = 11.78GVSAAAAFGAASVSAFYY323 pKa = 10.55RR324 pKa = 11.84AVTTDD329 pKa = 3.58FKK331 pKa = 11.68VAGTPDD337 pKa = 3.7NDD339 pKa = 3.24FEE341 pKa = 6.12AYY343 pKa = 9.86GLGVAYY349 pKa = 10.34DD350 pKa = 3.97LGGGLALEE358 pKa = 4.82AGVAQADD365 pKa = 3.69SDD367 pKa = 3.94RR368 pKa = 11.84AANALTVADD377 pKa = 4.74FGVSIDD383 pKa = 3.73FF384 pKa = 4.29

Molecular weight: 38.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2V2LEW7|A0A2V2LEW7_9RHOB Thioredoxin domain-containing protein OS=Rhodobacteraceae bacterium TG-679 OX=2072018 GN=DKT77_12545 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37AGRR28 pKa = 11.84KK29 pKa = 8.54ILNARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 8.43EE41 pKa = 3.72LSAA44 pKa = 5.03

Molecular weight: 5.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3860

0

3860

1231217

34

5831

319.0

34.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.568 ± 0.064

0.904 ± 0.013

6.34 ± 0.042

5.19 ± 0.042

3.618 ± 0.026

9.215 ± 0.045

1.98 ± 0.021

4.633 ± 0.031

2.386 ± 0.032

10.258 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.603 ± 0.022

2.295 ± 0.027

5.509 ± 0.03

2.958 ± 0.025

7.265 ± 0.048

4.867 ± 0.031

5.598 ± 0.037

7.487 ± 0.035

1.33 ± 0.018

1.994 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski