Clostridium indicum

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium

Average proteome isoelectric point is 6.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5501 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3E2NFP0|A0A3E2NFP0_9CLOT M3 family oligoendopeptidase OS=Clostridium indicum OX=759821 GN=DS742_06630 PE=3 SV=1
MM1 pKa = 7.21FTPKK5 pKa = 10.31NIQGALEE12 pKa = 4.12KK13 pKa = 10.68LYY15 pKa = 10.65DD16 pKa = 4.16LCDD19 pKa = 3.88PDD21 pKa = 5.15YY22 pKa = 10.8MVDD25 pKa = 3.24MLVNFSEE32 pKa = 4.43EE33 pKa = 3.92FDD35 pKa = 5.3DD36 pKa = 5.71ISPTLLARR44 pKa = 11.84SFQKK48 pKa = 10.26NAEE51 pKa = 4.31MVCEE55 pKa = 3.85YY56 pKa = 10.55RR57 pKa = 11.84VLSSAGEE64 pKa = 4.38GIDD67 pKa = 3.67YY68 pKa = 10.83QGTVLLNSRR77 pKa = 11.84AVRR80 pKa = 11.84LLSYY84 pKa = 10.75VEE86 pKa = 5.09DD87 pKa = 3.48MSGDD91 pKa = 3.5EE92 pKa = 4.42KK93 pKa = 11.44VRR95 pKa = 11.84TIQSKK100 pKa = 7.86EE101 pKa = 3.44LWLAEE106 pKa = 4.02DD107 pKa = 3.22MTFYY111 pKa = 11.0VVSCMSTITMDD122 pKa = 3.5KK123 pKa = 11.1EE124 pKa = 3.65EE125 pKa = 4.92AICLNEE131 pKa = 3.85HH132 pKa = 6.46RR133 pKa = 11.84SVVTTVEE140 pKa = 4.18CEE142 pKa = 3.67DD143 pKa = 5.83DD144 pKa = 3.43IFFDD148 pKa = 3.85MGSLICEE155 pKa = 4.64LDD157 pKa = 4.38DD158 pKa = 3.63ICLFEE163 pKa = 5.28LLADD167 pKa = 4.4ADD169 pKa = 3.76ATIYY173 pKa = 10.62EE174 pKa = 4.38LL175 pKa = 4.83

Molecular weight: 19.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3E2N765|A0A3E2N765_9CLOT Aminotransferase class V-fold PLP-dependent enzyme OS=Clostridium indicum OX=759821 GN=DS742_22230 PE=3 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 8.95KK9 pKa = 7.58RR10 pKa = 11.84QRR12 pKa = 11.84SKK14 pKa = 9.17VHH16 pKa = 5.89GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTPGGRR28 pKa = 11.84KK29 pKa = 8.8VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.33GRR39 pKa = 11.84AKK41 pKa = 10.69LSAA44 pKa = 3.92

Molecular weight: 4.98 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5501

0

5501

1752169

26

4801

318.5

35.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.162 ± 0.03

1.445 ± 0.015

5.491 ± 0.025

7.26 ± 0.037

4.249 ± 0.023

7.165 ± 0.034

1.715 ± 0.013

7.535 ± 0.033

6.819 ± 0.025

9.15 ± 0.034

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.064 ± 0.016

4.526 ± 0.023

3.412 ± 0.02

3.245 ± 0.021

4.368 ± 0.027

6.26 ± 0.029

5.448 ± 0.028

6.552 ± 0.028

0.972 ± 0.013

4.161 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski