Thalassobius mediterraneus

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Roseobacteraceae; Thalassobius

Average proteome isoelectric point is 6.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3260 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0P1GLN9|A0A0P1GLN9_9RHOB Glycerol-3-phosphate acyltransferase OS=Thalassobius mediterraneus OX=340021 GN=plsB PE=4 SV=1
MM1 pKa = 7.6PGNRR5 pKa = 11.84HH6 pKa = 5.04HH7 pKa = 7.17HH8 pKa = 6.06LFGLQGQLQGQGQMQGQGQHH28 pKa = 5.44QGQGQHH34 pKa = 5.56QGQGQGQHH42 pKa = 5.42QGQHH46 pKa = 5.45SEE48 pKa = 3.88NLNLNGNGNMNGNGNLNGNGNHH70 pKa = 6.68NGNEE74 pKa = 3.99NGNGNEE80 pKa = 4.1NHH82 pKa = 5.67NTNHH86 pKa = 5.69NTTSSDD92 pKa = 3.81AISAGLGMGVGAGLGHH108 pKa = 6.96AHH110 pKa = 7.27AGANSDD116 pKa = 3.97SGSHH120 pKa = 6.04SGSEE124 pKa = 4.18ASSSASTSNHH134 pKa = 4.97TDD136 pKa = 2.68VGVSVAVDD144 pKa = 3.62LTDD147 pKa = 4.34WEE149 pKa = 4.35PTDD152 pKa = 5.83DD153 pKa = 5.52DD154 pKa = 4.62FADD157 pKa = 4.13FDD159 pKa = 4.21LHH161 pKa = 6.94NSAVGDD167 pKa = 3.37ITMADD172 pKa = 3.23GDD174 pKa = 4.52MNTDD178 pKa = 2.47IGGNMSLDD186 pKa = 3.4GALNNALDD194 pKa = 4.09GAGNDD199 pKa = 3.46TGVINAMSNSLQDD212 pKa = 3.47NDD214 pKa = 3.86NLEE217 pKa = 4.45GASVSNGGSYY227 pKa = 8.66TATMTAHH234 pKa = 6.99GGSADD239 pKa = 3.72SEE241 pKa = 4.08EE242 pKa = 5.51GIDD245 pKa = 3.79NEE247 pKa = 4.76ADD249 pKa = 3.33GGSAGSAYY257 pKa = 10.69SDD259 pKa = 3.42GGKK262 pKa = 10.71GSTGNANGGHH272 pKa = 6.4SIALGGKK279 pKa = 9.5HH280 pKa = 5.81YY281 pKa = 11.14GNDD284 pKa = 3.67GGKK287 pKa = 9.52SEE289 pKa = 4.96GDD291 pKa = 3.6DD292 pKa = 4.37GGDD295 pKa = 3.42SGNAGAIAVGLGGLGLGLGGSADD318 pKa = 3.69GGTTGAGGAGGAGGTGGAGGSGGNGGNAGSAGGIGAAQGGDD359 pKa = 3.44SGALGAGAGGDD370 pKa = 3.5AGAIGAGAGGDD381 pKa = 3.53AGAVGAGLGGGLGLLPIGTGMGVGDD406 pKa = 4.33ADD408 pKa = 3.78AGNGNGTAGATAGAGTGTAGANAGNAGGYY437 pKa = 10.35GLGNSQSGGNDD448 pKa = 3.17ADD450 pKa = 4.19GAAGGAGAAGGAGAGSGTADD470 pKa = 2.93GGMAGGMGMGMGMGGGTAGAATGAGGAGGAGGDD503 pKa = 3.56AGASGGVAGGAVGYY517 pKa = 10.04GGHH520 pKa = 5.38ATIWGGNGGASGGSSADD537 pKa = 3.33GGAGGQTGGSWGSYY551 pKa = 9.88NGDD554 pKa = 3.31DD555 pKa = 4.02GFVNGYY561 pKa = 7.31STSSADD567 pKa = 3.71AALDD571 pKa = 3.43NAAFNQNIVQGANVLGNTVDD591 pKa = 3.48MTVVGGGYY599 pKa = 10.0SSTLIGDD606 pKa = 4.41DD607 pKa = 4.21DD608 pKa = 5.08LSS610 pKa = 3.89

Molecular weight: 55.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0P1GPW6|A0A0P1GPW6_9RHOB Phospho-2-dehydro-3-deoxyheptonate aldolase OS=Thalassobius mediterraneus OX=340021 GN=aroG PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 10.31QPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37AGRR28 pKa = 11.84KK29 pKa = 8.54ILNARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.07SLSAA44 pKa = 3.93

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3260

0

3260

1013264

29

2169

310.8

33.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.146 ± 0.054

0.912 ± 0.014

6.227 ± 0.038

5.896 ± 0.042

3.657 ± 0.027

8.441 ± 0.047

2.115 ± 0.023

5.099 ± 0.029

3.471 ± 0.04

10.117 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.923 ± 0.021

2.788 ± 0.022

4.805 ± 0.031

3.679 ± 0.024

6.278 ± 0.042

5.112 ± 0.027

5.458 ± 0.028

7.27 ± 0.038

1.33 ± 0.019

2.275 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski