Nocardiopsis sp. L17-MgMaSL7

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptosporangiales; Nocardiopsaceae; Nocardiopsis; unclassified Nocardiopsis

Average proteome isoelectric point is 5.96

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5387 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A317M2F9|A0A317M2F9_9ACTN Putative phenylalanine aminotransferase OS=Nocardiopsis sp. L17-MgMaSL7 OX=1938893 GN=pat PE=3 SV=1
MM1 pKa = 7.97RR2 pKa = 11.84SLRR5 pKa = 11.84LPAALAALVLVASACSGEE23 pKa = 4.3GAGGDD28 pKa = 3.26SGGYY32 pKa = 8.54PRR34 pKa = 11.84NEE36 pKa = 3.95TLYY39 pKa = 8.46TTGTAWGPPTNWNPIMEE56 pKa = 4.57GNYY59 pKa = 10.45AVGTVGLVYY68 pKa = 10.58EE69 pKa = 4.56PLFVFDD75 pKa = 4.85PSEE78 pKa = 3.91GAYY81 pKa = 9.12TPWLAEE87 pKa = 3.79SDD89 pKa = 3.69EE90 pKa = 4.36WPDD93 pKa = 3.41EE94 pKa = 4.11NTHH97 pKa = 6.67VITLRR102 pKa = 11.84DD103 pKa = 3.59GVEE106 pKa = 3.96WTDD109 pKa = 3.74GEE111 pKa = 4.4PLVAQDD117 pKa = 3.88VVTTLEE123 pKa = 4.37LGHH126 pKa = 6.69FDD128 pKa = 2.79IHH130 pKa = 7.27YY131 pKa = 10.35SNVWDD136 pKa = 3.74YY137 pKa = 11.86LEE139 pKa = 4.36SAEE142 pKa = 4.15ATGEE146 pKa = 4.17RR147 pKa = 11.84EE148 pKa = 4.03VTLTFSDD155 pKa = 4.06PRR157 pKa = 11.84PQEE160 pKa = 3.54WMNWVANRR168 pKa = 11.84AIVPDD173 pKa = 5.19HH174 pKa = 6.19IWADD178 pKa = 3.45KK179 pKa = 10.97SQEE182 pKa = 4.05QVNDD186 pKa = 3.6DD187 pKa = 4.05PNRR190 pKa = 11.84DD191 pKa = 3.56PVGTGAYY198 pKa = 9.74VYY200 pKa = 10.1EE201 pKa = 4.16NHH203 pKa = 7.25ADD205 pKa = 3.87DD206 pKa = 4.36RR207 pKa = 11.84MVWRR211 pKa = 11.84RR212 pKa = 11.84NDD214 pKa = 3.45DD215 pKa = 2.82WWGTEE220 pKa = 4.0ALDD223 pKa = 5.38LEE225 pKa = 4.68MKK227 pKa = 8.39PTYY230 pKa = 10.25IVDD233 pKa = 3.38IVNASNDD240 pKa = 3.05VTMNMLNNHH249 pKa = 5.79EE250 pKa = 4.48VDD252 pKa = 3.68LSNNFLPGIDD262 pKa = 3.38QVISNDD268 pKa = 3.53NNITSYY274 pKa = 10.73FAGPPYY280 pKa = 9.52MQSANTAWLVPNHH293 pKa = 5.85EE294 pKa = 4.71HH295 pKa = 6.98PALGDD300 pKa = 3.5PEE302 pKa = 4.18FRR304 pKa = 11.84RR305 pKa = 11.84ALAHH309 pKa = 7.15AVDD312 pKa = 4.32TSQIVEE318 pKa = 4.23GPYY321 pKa = 9.98TNLVEE326 pKa = 4.75AADD329 pKa = 4.01PTGLLPQWEE338 pKa = 4.62EE339 pKa = 4.31YY340 pKa = 9.9IDD342 pKa = 4.08HH343 pKa = 6.88EE344 pKa = 4.53LVADD348 pKa = 3.88EE349 pKa = 4.91GFTYY353 pKa = 10.71DD354 pKa = 3.28SGEE357 pKa = 3.81AVAILEE363 pKa = 4.2AAGYY367 pKa = 10.3LDD369 pKa = 4.02EE370 pKa = 7.35DD371 pKa = 4.02GDD373 pKa = 4.52GFVEE377 pKa = 4.7TPDD380 pKa = 4.14GEE382 pKa = 5.06PITLSLEE389 pKa = 4.45VPSGWSDD396 pKa = 2.52WMEE399 pKa = 4.05AANVIAEE406 pKa = 4.21NAQEE410 pKa = 4.02VGIKK414 pKa = 9.98VEE416 pKa = 3.99PAFPEE421 pKa = 4.17HH422 pKa = 6.8EE423 pKa = 4.02LLIEE427 pKa = 4.13NFRR430 pKa = 11.84KK431 pKa = 10.33AEE433 pKa = 3.95FDD435 pKa = 4.01LMINNDD441 pKa = 3.48RR442 pKa = 11.84SLSNTPWTYY451 pKa = 11.36YY452 pKa = 11.05DD453 pKa = 5.79FIFQLPIRR461 pKa = 11.84DD462 pKa = 3.67QQTTANYY469 pKa = 9.95ARR471 pKa = 11.84YY472 pKa = 8.89EE473 pKa = 4.01NEE475 pKa = 4.14EE476 pKa = 3.75AWEE479 pKa = 4.01LTEE482 pKa = 5.27QLAATPAEE490 pKa = 4.51DD491 pKa = 4.18AEE493 pKa = 4.48GMAAITSDD501 pKa = 3.5LQGIFLDD508 pKa = 4.62DD509 pKa = 4.47LPIIPLWYY517 pKa = 10.35NGMWSQASNQVWTNWPSEE535 pKa = 4.24DD536 pKa = 4.4EE537 pKa = 4.18GTPDD541 pKa = 4.72NFATMWNNMYY551 pKa = 10.99QMGAIYY557 pKa = 9.42TLAEE561 pKa = 4.05IEE563 pKa = 4.11LAEE566 pKa = 4.09

Molecular weight: 63.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A317MEQ0|A0A317MEQ0_9ACTN CP family cyanate transporter-like MFS transporter OS=Nocardiopsis sp. L17-MgMaSL7 OX=1938893 GN=BDW27_104267 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.99KK16 pKa = 9.72HH17 pKa = 5.74RR18 pKa = 11.84KK19 pKa = 7.77LLKK22 pKa = 7.46RR23 pKa = 11.84TRR25 pKa = 11.84VARR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.94KK32 pKa = 9.77

Molecular weight: 3.99 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5387

0

5387

1783097

27

4124

331.0

35.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.269 ± 0.044

0.695 ± 0.009

6.26 ± 0.028

6.618 ± 0.038

2.813 ± 0.019

9.462 ± 0.035

2.342 ± 0.018

3.135 ± 0.023

1.539 ± 0.021

10.413 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.88 ± 0.013

1.864 ± 0.019

6.088 ± 0.032

2.59 ± 0.02

8.154 ± 0.04

5.488 ± 0.024

5.954 ± 0.022

9.035 ± 0.033

1.492 ± 0.016

1.912 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski