Balaenoptera acutorostrata scammoni (North Pacific minke whale) (Balaenoptera davidsoni)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria; Eutheria; Boreoeutheria; Laurasiatheria; Artiodactyla; Whippomorpha;

Average proteome isoelectric point is 6.66

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 32130 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A384A451|A0A384A451_BALAS paralemmin-3 OS=Balaenoptera acutorostrata scammoni OX=310752 GN=PALM3 PE=4 SV=1
MM1 pKa = 7.33LHH3 pKa = 7.03LPQAWPPAGSCLSSEE18 pKa = 4.56CGPVRR23 pKa = 11.84TSRR26 pKa = 11.84EE27 pKa = 4.03QPVPTQPPRR36 pKa = 11.84QNPPEE41 pKa = 4.61DD42 pKa = 3.8NEE44 pKa = 4.46EE45 pKa = 4.25PPALPPRR52 pKa = 11.84TPEE55 pKa = 3.86GFQMEE60 pKa = 4.58EE61 pKa = 3.88EE62 pKa = 4.29PVYY65 pKa = 10.95EE66 pKa = 4.23EE67 pKa = 4.34APEE70 pKa = 4.23PEE72 pKa = 4.87PEE74 pKa = 4.44PEE76 pKa = 4.35PEE78 pKa = 4.21PEE80 pKa = 4.27PEE82 pKa = 3.79NDD84 pKa = 3.62YY85 pKa = 11.62EE86 pKa = 5.66DD87 pKa = 3.62VGEE90 pKa = 4.24MDD92 pKa = 2.8RR93 pKa = 11.84HH94 pKa = 5.87EE95 pKa = 4.7PDD97 pKa = 3.75EE98 pKa = 4.48EE99 pKa = 4.57PEE101 pKa = 3.93GDD103 pKa = 3.89YY104 pKa = 11.51EE105 pKa = 6.01DD106 pKa = 4.23VLEE109 pKa = 4.64PQDD112 pKa = 3.89PSFSSTLAGSSGCPAGAGAGISAVALYY139 pKa = 9.95DD140 pKa = 3.86YY141 pKa = 10.74QGEE144 pKa = 4.37GSDD147 pKa = 4.64EE148 pKa = 4.09ISFDD152 pKa = 3.81PDD154 pKa = 4.98DD155 pKa = 5.37IITDD159 pKa = 3.27IEE161 pKa = 4.37MVDD164 pKa = 3.5EE165 pKa = 4.67GWWRR169 pKa = 11.84GRR171 pKa = 11.84CHH173 pKa = 5.78GHH175 pKa = 6.85FGLFPANYY183 pKa = 7.78VTLLQQ188 pKa = 4.14

Molecular weight: 20.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A383YVM1|A0A383YVM1_BALAS zinc finger protein GLI4 OS=Balaenoptera acutorostrata scammoni OX=310752 GN=GLI4 PE=4 SV=2
MM1 pKa = 7.57SSHH4 pKa = 5.15KK5 pKa = 8.91TFRR8 pKa = 11.84IKK10 pKa = 10.64RR11 pKa = 11.84FLAKK15 pKa = 9.71KK16 pKa = 9.58QKK18 pKa = 8.69QNRR21 pKa = 11.84PIPQWIRR28 pKa = 11.84MKK30 pKa = 9.89TGNKK34 pKa = 8.61IRR36 pKa = 11.84YY37 pKa = 7.09NSKK40 pKa = 8.3RR41 pKa = 11.84RR42 pKa = 11.84HH43 pKa = 3.95WRR45 pKa = 11.84RR46 pKa = 11.84TKK48 pKa = 10.83LGLL51 pKa = 3.67

Molecular weight: 6.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

18711

13419

32130

20399175

31

34461

634.9

70.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.899 ± 0.013

2.128 ± 0.01

4.893 ± 0.012

7.343 ± 0.02

3.51 ± 0.01

6.389 ± 0.022

2.582 ± 0.007

4.288 ± 0.011

5.894 ± 0.02

9.857 ± 0.019

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.115 ± 0.006

3.609 ± 0.01

6.243 ± 0.024

4.917 ± 0.013

5.688 ± 0.011

8.478 ± 0.019

5.303 ± 0.009

6.011 ± 0.011

1.18 ± 0.005

2.59 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski