Sphingomonas solaris

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Sphingomonas

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4080 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A558RB38|A0A558RB38_9SPHN MFS transporter OS=Sphingomonas solaris OX=2529389 GN=FOY91_03600 PE=4 SV=1
VV1 pKa = 4.67VTGTGEE7 pKa = 3.74AGATVVISTADD18 pKa = 3.63GVVLGSKK25 pKa = 10.13VVGSDD30 pKa = 2.96GTYY33 pKa = 10.84LVVLDD38 pKa = 3.77PAQRR42 pKa = 11.84NGEE45 pKa = 4.17ALVATQRR52 pKa = 11.84DD53 pKa = 3.68PAGNVSDD60 pKa = 4.43PVGLSAPDD68 pKa = 3.46LTAPPPPGTLAVDD81 pKa = 3.98DD82 pKa = 5.68DD83 pKa = 4.3GTTLTGAGEE92 pKa = 3.87AGARR96 pKa = 11.84VEE98 pKa = 4.88VRR100 pKa = 11.84DD101 pKa = 4.14PDD103 pKa = 3.78GTLLGSGTVAPDD115 pKa = 3.48GSFTVEE121 pKa = 4.18LAPAQTAGEE130 pKa = 4.29TLMVTQADD138 pKa = 3.57AAGNVSGPASIDD150 pKa = 3.33APFDD154 pKa = 3.3VQAYY158 pKa = 10.37DD159 pKa = 3.45NFRR162 pKa = 11.84TASIDD167 pKa = 3.71LEE169 pKa = 4.35PTTTSVDD176 pKa = 3.37HH177 pKa = 7.01GSANYY182 pKa = 9.43LALVSLGLVNLQAEE196 pKa = 4.66VLSVNNVRR204 pKa = 11.84FSVQAGHH211 pKa = 6.56SLDD214 pKa = 5.38AEE216 pKa = 4.25FTYY219 pKa = 11.1NALLNIGVASGYY231 pKa = 9.79SVVVQRR237 pKa = 11.84LEE239 pKa = 3.98GTQWVAVSGTGPASVLEE256 pKa = 4.18LGLLNGDD263 pKa = 3.93LRR265 pKa = 11.84ATEE268 pKa = 4.1TFGPGEE274 pKa = 3.89YY275 pKa = 9.51RR276 pKa = 11.84AFLTFDD282 pKa = 3.49GAAGVGLLGNLNVTGSEE299 pKa = 4.08ADD301 pKa = 3.61FTANADD307 pKa = 3.62VTPIAATGNVIRR319 pKa = 11.84DD320 pKa = 3.78AGPDD324 pKa = 3.7GNVDD328 pKa = 3.19AVTPGTLLTSVTVNGVPIPVLPGGSTINTVWGTLTIAPDD367 pKa = 3.58GNYY370 pKa = 10.33SYY372 pKa = 11.3LPNADD377 pKa = 3.42PAAIGRR383 pKa = 11.84TEE385 pKa = 4.01VFTYY389 pKa = 10.55TLFDD393 pKa = 4.27PSSGQIEE400 pKa = 4.71SATLSITIDD409 pKa = 3.56SPDD412 pKa = 3.2IAGPPVAMPDD422 pKa = 3.43TAVAAVTFEE431 pKa = 4.18NVVAIAPSAPTFGFTSQPSGLLQSTGSGNGTFTVAANAEE470 pKa = 3.96ADD472 pKa = 3.4ITITAVRR479 pKa = 11.84QAGLSVSLLPTYY491 pKa = 9.95TVTLHH496 pKa = 6.33NADD499 pKa = 3.43NSVVRR504 pKa = 11.84TEE506 pKa = 4.28TVTALASALLGTAASFTFNDD526 pKa = 4.29LPGGAYY532 pKa = 8.87TYY534 pKa = 9.16TVSSSATTLGNFGTTVSLGSATTFLDD560 pKa = 4.16TYY562 pKa = 11.55ALATRR567 pKa = 11.84EE568 pKa = 4.33TVTGSLVDD576 pKa = 3.56NDD578 pKa = 3.98TTNTPFTTIRR588 pKa = 11.84INAGAGMVEE597 pKa = 4.29IDD599 pKa = 3.47QAPVSFAGQYY609 pKa = 7.52GTLTVDD615 pKa = 3.15QTGHH619 pKa = 4.73YY620 pKa = 8.36TYY622 pKa = 10.59QPHH625 pKa = 5.62EE626 pKa = 4.16TLAYY630 pKa = 10.16SATDD634 pKa = 3.41LVDD637 pKa = 3.09TFTYY641 pKa = 10.81QFVQPNGVVSVSTLNVTIDD660 pKa = 3.5VPGDD664 pKa = 3.52GPALVATTTSLAMAPSGHH682 pKa = 6.51EE683 pKa = 3.93GDD685 pKa = 5.25VIPLDD690 pKa = 4.08ALTHH694 pKa = 5.46QPGGDD699 pKa = 3.06AAVAQDD705 pKa = 3.74VAVSLATYY713 pKa = 10.73DD714 pKa = 3.71LFEE717 pKa = 4.58GQGDD721 pKa = 4.22LEE723 pKa = 4.85DD724 pKa = 3.73VLSNYY729 pKa = 10.19LSARR733 pKa = 11.84HH734 pKa = 6.59DD735 pKa = 3.82GATVVADD742 pKa = 3.65TASYY746 pKa = 10.68SSNSIDD752 pKa = 3.98LSGSTVTDD760 pKa = 3.55PFDD763 pKa = 3.72YY764 pKa = 10.88LVTVNDD770 pKa = 3.95HH771 pKa = 6.59DD772 pKa = 4.22HH773 pKa = 7.02DD774 pKa = 4.11RR775 pKa = 11.84TVSQHH780 pKa = 5.6VMM782 pKa = 3.19

Molecular weight: 79.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A558R008|A0A558R008_9SPHN 50S ribosomal protein L16 OS=Sphingomonas solaris OX=2529389 GN=rplP PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.32GFRR19 pKa = 11.84TRR21 pKa = 11.84SATPGGRR28 pKa = 11.84KK29 pKa = 8.76ILAARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84IKK41 pKa = 10.86LSAA44 pKa = 3.84

Molecular weight: 5.0 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4080

0

4080

1288240

29

1886

315.7

33.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.312 ± 0.062

0.763 ± 0.012

6.074 ± 0.028

4.984 ± 0.037

3.37 ± 0.021

9.25 ± 0.04

1.972 ± 0.021

4.773 ± 0.023

2.53 ± 0.026

9.896 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.298 ± 0.02

2.271 ± 0.026

5.561 ± 0.03

2.771 ± 0.021

7.919 ± 0.041

4.836 ± 0.03

5.529 ± 0.034

7.407 ± 0.027

1.365 ± 0.017

2.121 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski