Mycobacterium phage Eish

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Cheoctovirus; unclassified Cheoctovirus

Average proteome isoelectric point is 6.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 109 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A649V8C0|A0A649V8C0_9CAUD Uncharacterized protein OS=Mycobacterium phage Eish OX=2656575 GN=33 PE=4 SV=1
MM1 pKa = 7.52NNPEE5 pKa = 3.95LRR7 pKa = 11.84AVLTEE12 pKa = 3.82AQQAVIRR19 pKa = 11.84EE20 pKa = 4.17RR21 pKa = 11.84VSMALHH27 pKa = 6.02AAEE30 pKa = 4.86SNCLEE35 pKa = 4.15QGQVPGYY42 pKa = 10.43SAMAEE47 pKa = 3.95AAVTATCEE55 pKa = 4.09ALASLPGVAVIQLPEE70 pKa = 4.88PDD72 pKa = 5.07DD73 pKa = 3.77DD74 pKa = 5.7HH75 pKa = 9.06GDD77 pKa = 4.23GIAFWTAQEE86 pKa = 4.11VGVADD91 pKa = 3.82GMVVARR97 pKa = 11.84FDD99 pKa = 3.88PGNEE103 pKa = 3.83AIYY106 pKa = 10.33YY107 pKa = 7.43EE108 pKa = 4.59PSEE111 pKa = 4.01AWEE114 pKa = 4.37TVAVLAAAAVVAEE127 pKa = 4.87GEE129 pKa = 4.39DD130 pKa = 3.55KK131 pKa = 11.29

Molecular weight: 13.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A649V7X2|A0A649V7X2_9CAUD Uncharacterized protein OS=Mycobacterium phage Eish OX=2656575 GN=102 PE=4 SV=1
MM1 pKa = 8.05PDD3 pKa = 3.22RR4 pKa = 11.84YY5 pKa = 10.68GDD7 pKa = 3.63PTPEE11 pKa = 3.56PRR13 pKa = 11.84VFVRR17 pKa = 11.84PKK19 pKa = 10.42VNALTVRR26 pKa = 11.84CSWCKK31 pKa = 9.95AGVGSRR37 pKa = 11.84CVVAGTSVVLRR48 pKa = 11.84RR49 pKa = 11.84SSFHH53 pKa = 6.57DD54 pKa = 3.25VRR56 pKa = 11.84VRR58 pKa = 11.84DD59 pKa = 3.96AEE61 pKa = 4.18LAATGALTRR70 pKa = 11.84GRR72 pKa = 11.84MSS74 pKa = 2.99

Molecular weight: 8.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

109

0

109

18601

28

1223

170.7

18.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.252 ± 0.481

1.312 ± 0.188

6.537 ± 0.239

5.892 ± 0.29

3.005 ± 0.191

8.897 ± 0.57

2.258 ± 0.194

4.263 ± 0.181

3.408 ± 0.193

7.338 ± 0.223

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.247 ± 0.131

3.36 ± 0.156

6.107 ± 0.202

3.425 ± 0.17

6.94 ± 0.418

5.817 ± 0.291

6.844 ± 0.259

7.258 ± 0.263

2.258 ± 0.142

2.581 ± 0.153
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski