Amino acid dipepetide frequency for Mycobacterium phage Eish

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.634AlaAla: 12.634 ± 1.435
1.075AlaCys: 1.075 ± 0.241
6.828AlaAsp: 6.828 ± 0.544
7.473AlaGlu: 7.473 ± 0.703
2.527AlaPhe: 2.527 ± 0.329
9.462AlaGly: 9.462 ± 1.246
2.366AlaHis: 2.366 ± 0.457
4.409AlaIle: 4.409 ± 0.506
4.355AlaLys: 4.355 ± 0.471
7.796AlaLeu: 7.796 ± 0.801
2.419AlaMet: 2.419 ± 0.397
2.634AlaAsn: 2.634 ± 0.428
5.323AlaPro: 5.323 ± 0.581
3.925AlaGln: 3.925 ± 0.435
6.505AlaArg: 6.505 ± 0.701
5.161AlaSer: 5.161 ± 0.522
5.753AlaThr: 5.753 ± 0.608
7.097AlaVal: 7.097 ± 0.658
2.419AlaTrp: 2.419 ± 0.36
2.366AlaTyr: 2.366 ± 0.421
0.0AlaXaa: 0.0 ± 0.0
Cys
0.968CysAla: 0.968 ± 0.271
0.054CysCys: 0.054 ± 0.062
1.129CysAsp: 1.129 ± 0.296
1.183CysGlu: 1.183 ± 0.275
0.269CysPhe: 0.269 ± 0.11
2.097CysGly: 2.097 ± 0.51
0.323CysHis: 0.323 ± 0.138
0.108CysIle: 0.108 ± 0.095
0.484CysLys: 0.484 ± 0.16
0.645CysLeu: 0.645 ± 0.232
0.161CysMet: 0.161 ± 0.081
0.43CysAsn: 0.43 ± 0.169
1.29CysPro: 1.29 ± 0.263
0.376CysGln: 0.376 ± 0.124
0.699CysArg: 0.699 ± 0.212
0.699CysSer: 0.699 ± 0.24
0.86CysThr: 0.86 ± 0.282
0.806CysVal: 0.806 ± 0.185
0.323CysTrp: 0.323 ± 0.126
0.215CysTyr: 0.215 ± 0.101
0.0CysXaa: 0.0 ± 0.0
Asp
6.72AspAla: 6.72 ± 0.681
1.129AspCys: 1.129 ± 0.26
4.731AspAsp: 4.731 ± 0.552
3.172AspGlu: 3.172 ± 0.474
1.774AspPhe: 1.774 ± 0.256
6.075AspGly: 6.075 ± 0.654
1.129AspHis: 1.129 ± 0.24
2.204AspIle: 2.204 ± 0.325
1.613AspLys: 1.613 ± 0.284
5.645AspLeu: 5.645 ± 0.478
1.075AspMet: 1.075 ± 0.277
1.72AspAsn: 1.72 ± 0.373
5.054AspPro: 5.054 ± 0.585
2.634AspGln: 2.634 ± 0.398
5.376AspArg: 5.376 ± 0.654
2.849AspSer: 2.849 ± 0.443
4.624AspThr: 4.624 ± 0.467
4.247AspVal: 4.247 ± 0.451
1.505AspTrp: 1.505 ± 0.278
2.097AspTyr: 2.097 ± 0.299
0.0AspXaa: 0.0 ± 0.0
Glu
6.505GluAla: 6.505 ± 0.674
0.86GluCys: 0.86 ± 0.251
3.118GluAsp: 3.118 ± 0.381
2.258GluGlu: 2.258 ± 0.489
2.043GluPhe: 2.043 ± 0.315
3.548GluGly: 3.548 ± 0.364
1.559GluHis: 1.559 ± 0.351
2.258GluIle: 2.258 ± 0.332
1.935GluLys: 1.935 ± 0.292
5.806GluLeu: 5.806 ± 0.658
1.344GluMet: 1.344 ± 0.253
1.989GluAsn: 1.989 ± 0.302
3.172GluPro: 3.172 ± 0.449
2.796GluGln: 2.796 ± 0.413
5.323GluArg: 5.323 ± 0.603
2.957GluSer: 2.957 ± 0.44
4.14GluThr: 4.14 ± 0.565
4.247GluVal: 4.247 ± 0.537
1.183GluTrp: 1.183 ± 0.226
1.882GluTyr: 1.882 ± 0.349
0.0GluXaa: 0.0 ± 0.0
Phe
2.742PheAla: 2.742 ± 0.326
0.269PheCys: 0.269 ± 0.12
2.742PheAsp: 2.742 ± 0.503
1.559PheGlu: 1.559 ± 0.321
0.86PhePhe: 0.86 ± 0.218
3.172PheGly: 3.172 ± 0.587
0.538PheHis: 0.538 ± 0.188
1.505PheIle: 1.505 ± 0.334
1.075PheLys: 1.075 ± 0.258
1.774PheLeu: 1.774 ± 0.309
0.753PheMet: 0.753 ± 0.181
1.29PheAsn: 1.29 ± 0.256
1.72PhePro: 1.72 ± 0.307
1.022PheGln: 1.022 ± 0.328
1.559PheArg: 1.559 ± 0.261
1.613PheSer: 1.613 ± 0.312
2.366PheThr: 2.366 ± 0.417
1.935PheVal: 1.935 ± 0.248
0.753PheTrp: 0.753 ± 0.191
0.806PheTyr: 0.806 ± 0.271
0.0PheXaa: 0.0 ± 0.0
Gly
8.441GlyAla: 8.441 ± 1.193
1.398GlyCys: 1.398 ± 0.329
5.484GlyAsp: 5.484 ± 0.532
4.409GlyGlu: 4.409 ± 0.492
2.796GlyPhe: 2.796 ± 0.521
11.183GlyGly: 11.183 ± 2.015
1.828GlyHis: 1.828 ± 0.311
4.032GlyIle: 4.032 ± 0.545
2.796GlyLys: 2.796 ± 0.392
5.968GlyLeu: 5.968 ± 0.567
2.258GlyMet: 2.258 ± 0.431
3.28GlyAsn: 3.28 ± 0.422
3.978GlyPro: 3.978 ± 0.733
2.204GlyGln: 2.204 ± 0.535
5.269GlyArg: 5.269 ± 0.613
6.398GlySer: 6.398 ± 0.779
6.29GlyThr: 6.29 ± 0.749
6.129GlyVal: 6.129 ± 0.603
2.581GlyTrp: 2.581 ± 0.37
2.258GlyTyr: 2.258 ± 0.404
0.0GlyXaa: 0.0 ± 0.0
His
2.043HisAla: 2.043 ± 0.366
0.43HisCys: 0.43 ± 0.192
1.129HisAsp: 1.129 ± 0.254
1.29HisGlu: 1.29 ± 0.296
0.43HisPhe: 0.43 ± 0.127
1.72HisGly: 1.72 ± 0.262
0.645HisHis: 0.645 ± 0.21
1.29HisIle: 1.29 ± 0.243
0.968HisLys: 0.968 ± 0.245
1.344HisLeu: 1.344 ± 0.249
0.538HisMet: 0.538 ± 0.165
0.86HisAsn: 0.86 ± 0.238
1.29HisPro: 1.29 ± 0.238
0.699HisGln: 0.699 ± 0.2
2.312HisArg: 2.312 ± 0.465
0.968HisSer: 0.968 ± 0.226
1.828HisThr: 1.828 ± 0.445
1.344HisVal: 1.344 ± 0.34
0.484HisTrp: 0.484 ± 0.15
0.968HisTyr: 0.968 ± 0.21
0.0HisXaa: 0.0 ± 0.0
Ile
5.0IleAla: 5.0 ± 0.571
0.753IleCys: 0.753 ± 0.245
3.387IleAsp: 3.387 ± 0.465
3.065IleGlu: 3.065 ± 0.329
0.699IlePhe: 0.699 ± 0.216
4.194IleGly: 4.194 ± 0.478
1.29IleHis: 1.29 ± 0.288
1.344IleIle: 1.344 ± 0.292
1.075IleLys: 1.075 ± 0.267
2.097IleLeu: 2.097 ± 0.362
0.43IleMet: 0.43 ± 0.129
1.72IleAsn: 1.72 ± 0.28
2.742IlePro: 2.742 ± 0.329
1.505IleGln: 1.505 ± 0.277
2.742IleArg: 2.742 ± 0.397
2.043IleSer: 2.043 ± 0.407
3.441IleThr: 3.441 ± 0.445
3.172IleVal: 3.172 ± 0.375
0.86IleTrp: 0.86 ± 0.21
1.075IleTyr: 1.075 ± 0.228
0.0IleXaa: 0.0 ± 0.0
Lys
4.032LysAla: 4.032 ± 0.551
0.376LysCys: 0.376 ± 0.132
1.828LysAsp: 1.828 ± 0.294
1.505LysGlu: 1.505 ± 0.288
1.398LysPhe: 1.398 ± 0.215
2.634LysGly: 2.634 ± 0.36
1.129LysHis: 1.129 ± 0.273
0.968LysIle: 0.968 ± 0.224
1.344LysLys: 1.344 ± 0.401
2.527LysLeu: 2.527 ± 0.428
0.806LysMet: 0.806 ± 0.189
0.968LysAsn: 0.968 ± 0.224
2.204LysPro: 2.204 ± 0.313
1.667LysGln: 1.667 ± 0.217
2.312LysArg: 2.312 ± 0.305
2.097LysSer: 2.097 ± 0.341
2.258LysThr: 2.258 ± 0.326
2.312LysVal: 2.312 ± 0.347
0.86LysTrp: 0.86 ± 0.226
0.86LysTyr: 0.86 ± 0.249
0.0LysXaa: 0.0 ± 0.0
Leu
7.903LeuAla: 7.903 ± 0.829
0.645LeuCys: 0.645 ± 0.238
4.785LeuAsp: 4.785 ± 0.502
3.925LeuGlu: 3.925 ± 0.466
2.473LeuPhe: 2.473 ± 0.35
5.376LeuGly: 5.376 ± 0.608
1.237LeuHis: 1.237 ± 0.274
2.903LeuIle: 2.903 ± 0.469
2.097LeuLys: 2.097 ± 0.326
4.247LeuLeu: 4.247 ± 0.477
1.774LeuMet: 1.774 ± 0.278
2.366LeuAsn: 2.366 ± 0.363
5.914LeuPro: 5.914 ± 0.678
2.903LeuGln: 2.903 ± 0.448
5.323LeuArg: 5.323 ± 0.653
5.0LeuSer: 5.0 ± 0.541
5.753LeuThr: 5.753 ± 0.494
5.054LeuVal: 5.054 ± 0.53
1.505LeuTrp: 1.505 ± 0.329
2.204LeuTyr: 2.204 ± 0.363
0.0LeuXaa: 0.0 ± 0.0
Met
2.097MetAla: 2.097 ± 0.344
0.215MetCys: 0.215 ± 0.121
1.398MetAsp: 1.398 ± 0.288
1.022MetGlu: 1.022 ± 0.186
0.645MetPhe: 0.645 ± 0.195
1.774MetGly: 1.774 ± 0.308
0.161MetHis: 0.161 ± 0.1
0.86MetIle: 0.86 ± 0.223
0.753MetLys: 0.753 ± 0.202
2.097MetLeu: 2.097 ± 0.331
0.484MetMet: 0.484 ± 0.221
0.753MetAsn: 0.753 ± 0.165
1.344MetPro: 1.344 ± 0.261
0.484MetGln: 0.484 ± 0.15
1.559MetArg: 1.559 ± 0.274
2.796MetSer: 2.796 ± 0.361
1.989MetThr: 1.989 ± 0.273
1.452MetVal: 1.452 ± 0.331
0.215MetTrp: 0.215 ± 0.106
0.376MetTyr: 0.376 ± 0.134
0.0MetXaa: 0.0 ± 0.0
Asn
3.441AsnAla: 3.441 ± 0.418
0.43AsnCys: 0.43 ± 0.178
1.828AsnAsp: 1.828 ± 0.312
1.828AsnGlu: 1.828 ± 0.4
0.699AsnPhe: 0.699 ± 0.262
3.925AsnGly: 3.925 ± 0.584
1.075AsnHis: 1.075 ± 0.237
1.667AsnIle: 1.667 ± 0.375
0.914AsnLys: 0.914 ± 0.232
2.742AsnLeu: 2.742 ± 0.396
0.591AsnMet: 0.591 ± 0.162
1.505AsnAsn: 1.505 ± 0.279
2.473AsnPro: 2.473 ± 0.345
1.129AsnGln: 1.129 ± 0.28
2.204AsnArg: 2.204 ± 0.409
1.452AsnSer: 1.452 ± 0.268
2.151AsnThr: 2.151 ± 0.374
2.097AsnVal: 2.097 ± 0.334
0.753AsnTrp: 0.753 ± 0.209
0.699AsnTyr: 0.699 ± 0.167
0.0AsnXaa: 0.0 ± 0.0
Pro
5.43ProAla: 5.43 ± 0.649
0.753ProCys: 0.753 ± 0.206
4.409ProAsp: 4.409 ± 0.508
4.677ProGlu: 4.677 ± 0.529
2.097ProPhe: 2.097 ± 0.347
6.183ProGly: 6.183 ± 0.688
1.452ProHis: 1.452 ± 0.299
2.043ProIle: 2.043 ± 0.376
2.473ProLys: 2.473 ± 0.381
4.462ProLeu: 4.462 ± 0.553
1.72ProMet: 1.72 ± 0.347
2.204ProAsn: 2.204 ± 0.286
4.194ProPro: 4.194 ± 0.644
1.989ProGln: 1.989 ± 0.332
3.118ProArg: 3.118 ± 0.496
3.71ProSer: 3.71 ± 0.405
3.333ProThr: 3.333 ± 0.497
4.247ProVal: 4.247 ± 0.504
1.075ProTrp: 1.075 ± 0.241
1.505ProTyr: 1.505 ± 0.251
0.0ProXaa: 0.0 ± 0.0
Gln
3.978GlnAla: 3.978 ± 0.517
0.376GlnCys: 0.376 ± 0.161
1.613GlnAsp: 1.613 ± 0.27
1.774GlnGlu: 1.774 ± 0.337
1.075GlnPhe: 1.075 ± 0.202
2.581GlnGly: 2.581 ± 0.447
0.753GlnHis: 0.753 ± 0.223
1.774GlnIle: 1.774 ± 0.359
1.398GlnLys: 1.398 ± 0.249
3.118GlnLeu: 3.118 ± 0.449
0.699GlnMet: 0.699 ± 0.221
0.806GlnAsn: 0.806 ± 0.229
2.312GlnPro: 2.312 ± 0.37
1.237GlnGln: 1.237 ± 0.322
2.581GlnArg: 2.581 ± 0.405
2.097GlnSer: 2.097 ± 0.396
2.043GlnThr: 2.043 ± 0.377
2.473GlnVal: 2.473 ± 0.34
0.645GlnTrp: 0.645 ± 0.18
0.86GlnTyr: 0.86 ± 0.259
0.0GlnXaa: 0.0 ± 0.0
Arg
6.935ArgAla: 6.935 ± 0.59
1.398ArgCys: 1.398 ± 0.345
4.516ArgAsp: 4.516 ± 0.516
5.376ArgGlu: 5.376 ± 0.769
2.366ArgPhe: 2.366 ± 0.428
3.656ArgGly: 3.656 ± 0.396
1.398ArgHis: 1.398 ± 0.348
4.194ArgIle: 4.194 ± 0.606
2.258ArgLys: 2.258 ± 0.307
5.269ArgLeu: 5.269 ± 0.619
2.527ArgMet: 2.527 ± 0.398
2.527ArgAsn: 2.527 ± 0.416
3.495ArgPro: 3.495 ± 0.528
1.667ArgGln: 1.667 ± 0.277
5.161ArgArg: 5.161 ± 0.738
3.925ArgSer: 3.925 ± 0.44
3.548ArgThr: 3.548 ± 0.565
5.591ArgVal: 5.591 ± 0.683
1.935ArgTrp: 1.935 ± 0.315
1.667ArgTyr: 1.667 ± 0.265
0.0ArgXaa: 0.0 ± 0.0
Ser
5.108SerAla: 5.108 ± 0.78
0.376SerCys: 0.376 ± 0.178
3.817SerAsp: 3.817 ± 0.439
3.118SerGlu: 3.118 ± 0.436
2.043SerPhe: 2.043 ± 0.423
6.129SerGly: 6.129 ± 0.78
1.29SerHis: 1.29 ± 0.271
2.903SerIle: 2.903 ± 0.412
2.258SerLys: 2.258 ± 0.358
3.817SerLeu: 3.817 ± 0.475
1.075SerMet: 1.075 ± 0.261
2.903SerAsn: 2.903 ± 0.43
3.333SerPro: 3.333 ± 0.357
1.505SerGln: 1.505 ± 0.312
3.387SerArg: 3.387 ± 0.367
3.71SerSer: 3.71 ± 0.717
3.656SerThr: 3.656 ± 0.501
4.946SerVal: 4.946 ± 0.721
1.29SerTrp: 1.29 ± 0.222
1.505SerTyr: 1.505 ± 0.294
0.0SerXaa: 0.0 ± 0.0
Thr
6.667ThrAla: 6.667 ± 0.62
0.753ThrCys: 0.753 ± 0.243
4.301ThrAsp: 4.301 ± 0.611
3.656ThrGlu: 3.656 ± 0.427
1.935ThrPhe: 1.935 ± 0.282
6.667ThrGly: 6.667 ± 0.743
1.882ThrHis: 1.882 ± 0.358
3.172ThrIle: 3.172 ± 0.486
2.419ThrLys: 2.419 ± 0.391
4.462ThrLeu: 4.462 ± 0.59
1.237ThrMet: 1.237 ± 0.273
2.097ThrAsn: 2.097 ± 0.335
4.624ThrPro: 4.624 ± 0.532
1.935ThrGln: 1.935 ± 0.346
4.247ThrArg: 4.247 ± 0.54
3.871ThrSer: 3.871 ± 0.485
5.323ThrThr: 5.323 ± 0.763
5.86ThrVal: 5.86 ± 0.583
1.183ThrTrp: 1.183 ± 0.277
2.151ThrTyr: 2.151 ± 0.336
0.0ThrXaa: 0.0 ± 0.0
Val
7.258ValAla: 7.258 ± 0.555
1.29ValCys: 1.29 ± 0.227
5.323ValAsp: 5.323 ± 0.556
4.785ValGlu: 4.785 ± 0.59
2.473ValPhe: 2.473 ± 0.428
5.484ValGly: 5.484 ± 0.591
1.398ValHis: 1.398 ± 0.29
2.688ValIle: 2.688 ± 0.458
2.366ValLys: 2.366 ± 0.389
5.269ValLeu: 5.269 ± 0.622
1.29ValMet: 1.29 ± 0.235
2.204ValAsn: 2.204 ± 0.389
4.194ValPro: 4.194 ± 0.394
2.742ValGln: 2.742 ± 0.348
5.054ValArg: 5.054 ± 0.587
4.624ValSer: 4.624 ± 0.609
5.645ValThr: 5.645 ± 0.497
5.591ValVal: 5.591 ± 0.615
1.613ValTrp: 1.613 ± 0.323
1.29ValTyr: 1.29 ± 0.259
0.0ValXaa: 0.0 ± 0.0
Trp
2.204TrpAla: 2.204 ± 0.369
0.269TrpCys: 0.269 ± 0.115
1.344TrpAsp: 1.344 ± 0.282
1.022TrpGlu: 1.022 ± 0.322
0.645TrpPhe: 0.645 ± 0.192
0.753TrpGly: 0.753 ± 0.214
0.753TrpHis: 0.753 ± 0.191
1.022TrpIle: 1.022 ± 0.257
0.806TrpLys: 0.806 ± 0.176
1.828TrpLeu: 1.828 ± 0.283
1.022TrpMet: 1.022 ± 0.242
0.591TrpAsn: 0.591 ± 0.202
1.129TrpPro: 1.129 ± 0.265
0.914TrpGln: 0.914 ± 0.256
2.258TrpArg: 2.258 ± 0.415
1.344TrpSer: 1.344 ± 0.233
1.559TrpThr: 1.559 ± 0.306
1.828TrpVal: 1.828 ± 0.401
0.753TrpTrp: 0.753 ± 0.161
0.538TrpTyr: 0.538 ± 0.179
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.419TyrAla: 2.419 ± 0.35
0.269TyrCys: 0.269 ± 0.136
1.667TyrAsp: 1.667 ± 0.356
1.828TyrGlu: 1.828 ± 0.321
0.806TyrPhe: 0.806 ± 0.163
2.366TyrGly: 2.366 ± 0.487
0.376TyrHis: 0.376 ± 0.138
1.29TyrIle: 1.29 ± 0.214
0.699TyrLys: 0.699 ± 0.212
2.473TyrLeu: 2.473 ± 0.415
0.215TyrMet: 0.215 ± 0.099
0.753TyrAsn: 0.753 ± 0.159
1.29TyrPro: 1.29 ± 0.22
0.914TyrGln: 0.914 ± 0.199
2.419TyrArg: 2.419 ± 0.364
0.86TyrSer: 0.86 ± 0.229
1.882TyrThr: 1.882 ± 0.36
2.151TyrVal: 2.151 ± 0.283
0.645TyrTrp: 0.645 ± 0.213
0.484TyrTyr: 0.484 ± 0.155
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 109 proteins (18601 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski