Canine mastadenovirus A
Average proteome isoelectric point is 6.45
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 31 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6S6MM40|A0A6S6MM40_9ADEN E4 ORF A OS=Canine mastadenovirus A OX=10537 PE=4 SV=1
MM1 pKa = 7.96 KK2 pKa = 9.44 YY3 pKa = 9.12 TIVPAPRR10 pKa = 11.84 NLHH13 pKa = 6.86 DD14 pKa = 4.27 YY15 pKa = 10.64 VLEE18 pKa = 4.72 LLEE21 pKa = 4.54 EE22 pKa = 4.15 WQPDD26 pKa = 4.08 CLDD29 pKa = 3.45 CEE31 pKa = 4.57 YY32 pKa = 10.51 PHH34 pKa = 7.12 GSPSPPTLHH43 pKa = 7.48 DD44 pKa = 4.17 LFDD47 pKa = 4.14 VEE49 pKa = 5.88 LEE51 pKa = 4.34 TSHH54 pKa = 7.55 SPFVGLCDD62 pKa = 3.43 SCAEE66 pKa = 4.27 ADD68 pKa = 3.78 TDD70 pKa = 4.26 SSASTEE76 pKa = 4.03 ADD78 pKa = 3.22 SGFSPLSTPPVSPIPPHH95 pKa = 5.76 PTSPASISDD104 pKa = 3.82 DD105 pKa = 3.14 MLLCLEE111 pKa = 4.63 EE112 pKa = 4.67 MPTFDD117 pKa = 6.17 DD118 pKa = 3.65 EE119 pKa = 5.53 DD120 pKa = 4.02 EE121 pKa = 4.35 VRR123 pKa = 11.84 SAATTFEE130 pKa = 3.86 RR131 pKa = 11.84 WEE133 pKa = 4.09 NTFDD137 pKa = 3.56 PHH139 pKa = 6.94 VGPIFGCLRR148 pKa = 11.84 CAFYY152 pKa = 10.82 QEE154 pKa = 4.06 QDD156 pKa = 3.54 DD157 pKa = 4.13 NALCGLCYY165 pKa = 10.43 LKK167 pKa = 10.94 ALAEE171 pKa = 4.38 VPFALPVRR179 pKa = 11.84 SEE181 pKa = 3.97 PASAGAEE188 pKa = 4.06 EE189 pKa = 4.89 EE190 pKa = 3.99 DD191 pKa = 4.47 DD192 pKa = 3.85 EE193 pKa = 5.88 VIFVSAKK200 pKa = 8.33 PGGRR204 pKa = 11.84 KK205 pKa = 9.11 RR206 pKa = 11.84 SAATPCEE213 pKa = 3.72 PDD215 pKa = 3.5 GASKK219 pKa = 10.52 RR220 pKa = 11.84 PCVPEE225 pKa = 4.13 PEE227 pKa = 4.22 QTEE230 pKa = 4.07 PLDD233 pKa = 4.45 LSLKK237 pKa = 9.67 PRR239 pKa = 11.84 PNN241 pKa = 3.21
Molecular weight: 26.3 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.865
IPC2_protein 4.113
IPC_protein 4.075
Toseland 3.897
ProMoST 4.202
Dawson 4.037
Bjellqvist 4.19
Wikipedia 3.923
Rodwell 3.91
Grimsley 3.808
Solomon 4.037
Lehninger 3.986
Nozaki 4.151
DTASelect 4.329
Thurlkill 3.923
EMBOSS 3.948
Sillero 4.19
Patrickios 1.939
IPC_peptide 4.037
IPC2_peptide 4.177
IPC2.peptide.svr19 4.07
Protein with the highest isoelectric point:
>tr|A0A6S6MFM5|A0A6S6MFM5_9ADEN E3 ORF A OS=Canine mastadenovirus A OX=10537 PE=4 SV=1
MM1 pKa = 7.77 AGRR4 pKa = 11.84 NVTLRR9 pKa = 11.84 LRR11 pKa = 11.84 VPVRR15 pKa = 11.84 TKK17 pKa = 9.3 ITGAGRR23 pKa = 11.84 RR24 pKa = 11.84 RR25 pKa = 11.84 GRR27 pKa = 11.84 RR28 pKa = 11.84 PRR30 pKa = 11.84 GIRR33 pKa = 11.84 CGRR36 pKa = 11.84 MRR38 pKa = 11.84 GGFLPALIPLIAAAIGAVPGIASVALQAARR68 pKa = 11.84 HH69 pKa = 4.96
Molecular weight: 7.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.463
IPC2_protein 11.008
IPC_protein 12.618
Toseland 12.778
ProMoST 13.276
Dawson 12.778
Bjellqvist 12.778
Wikipedia 13.261
Rodwell 12.31
Grimsley 12.822
Solomon 13.276
Lehninger 13.173
Nozaki 12.778
DTASelect 12.778
Thurlkill 12.778
EMBOSS 13.276
Sillero 12.778
Patrickios 12.047
IPC_peptide 13.276
IPC2_peptide 12.266
IPC2.peptide.svr19 9.149
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
27
4
31
10145
55
1150
327.3
36.65
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.777 ± 0.339
2.04 ± 0.309
4.406 ± 0.22
6.18 ± 0.45
4.268 ± 0.192
5.786 ± 0.36
2.326 ± 0.201
4.406 ± 0.213
4.593 ± 0.498
9.216 ± 0.345
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.642 ± 0.172
5.126 ± 0.431
6.516 ± 0.404
4.317 ± 0.211
5.954 ± 0.521
7.186 ± 0.234
6.082 ± 0.338
6.614 ± 0.292
1.242 ± 0.096
3.322 ± 0.329
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here