Butyrivibrio sp. TB

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Butyrivibrio; unclassified Butyrivibrio

Average proteome isoelectric point is 5.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3714 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H9GRL2|A0A1H9GRL2_9FIRM Non-specific serine/threonine protein kinase OS=Butyrivibrio sp. TB OX=1520809 GN=SAMN02910382_03317 PE=4 SV=1
MM1 pKa = 7.78KK2 pKa = 10.29KK3 pKa = 10.36RR4 pKa = 11.84IISVMLGAMILCAGCGAAINIEE26 pKa = 3.98EE27 pKa = 4.69DD28 pKa = 3.87EE29 pKa = 4.35ITSEE33 pKa = 4.18DD34 pKa = 3.55KK35 pKa = 11.44VEE37 pKa = 3.98EE38 pKa = 4.17FEE40 pKa = 4.37DD41 pKa = 3.83AKK43 pKa = 11.1SEE45 pKa = 4.17SSNEE49 pKa = 3.7VATISSEE56 pKa = 3.93EE57 pKa = 4.23SSVDD61 pKa = 3.08TSEE64 pKa = 5.28EE65 pKa = 3.45IDD67 pKa = 3.41ITDD70 pKa = 4.57EE71 pKa = 4.54EE72 pKa = 5.71DD73 pKa = 3.52DD74 pKa = 3.95TTKK77 pKa = 10.59DD78 pKa = 2.92TDD80 pKa = 3.45TSKK83 pKa = 11.59DD84 pKa = 3.52EE85 pKa = 5.07DD86 pKa = 4.45DD87 pKa = 3.84STNDD91 pKa = 3.0VSRR94 pKa = 11.84YY95 pKa = 8.57EE96 pKa = 4.27SFVSNTEE103 pKa = 3.65EE104 pKa = 4.22VFFDD108 pKa = 3.82EE109 pKa = 4.89SGSDD113 pKa = 3.21ILPYY117 pKa = 10.39SLQRR121 pKa = 11.84NFDD124 pKa = 3.44LSKK127 pKa = 10.83GYY129 pKa = 8.93TIDD132 pKa = 4.22EE133 pKa = 4.19IVEE136 pKa = 4.17ASLDD140 pKa = 3.9SYY142 pKa = 11.21TSYY145 pKa = 11.52DD146 pKa = 3.85DD147 pKa = 5.39SKK149 pKa = 11.5DD150 pKa = 3.65DD151 pKa = 4.05PSFEE155 pKa = 4.33TKK157 pKa = 10.87YY158 pKa = 10.37IDD160 pKa = 4.82CGNDD164 pKa = 3.94GIDD167 pKa = 3.84EE168 pKa = 4.58LLVDD172 pKa = 3.62IRR174 pKa = 11.84FPGMEE179 pKa = 3.92TADD182 pKa = 3.47YY183 pKa = 10.55TDD185 pKa = 3.88YY186 pKa = 11.36EE187 pKa = 4.39EE188 pKa = 6.06KK189 pKa = 10.96YY190 pKa = 9.49VDD192 pKa = 2.68RR193 pKa = 11.84WVIKK197 pKa = 10.51EE198 pKa = 4.08IDD200 pKa = 3.71DD201 pKa = 3.89KK202 pKa = 11.87LIVKK206 pKa = 9.2YY207 pKa = 10.75VGALRR212 pKa = 11.84HH213 pKa = 5.6EE214 pKa = 5.09SYY216 pKa = 10.67TIINDD221 pKa = 3.06YY222 pKa = 11.06GYY224 pKa = 10.5IKK226 pKa = 10.6NEE228 pKa = 3.49VMGAYY233 pKa = 10.12NGVLSEE239 pKa = 4.35SYY241 pKa = 10.97GYY243 pKa = 11.2LDD245 pKa = 5.67ADD247 pKa = 4.51ANWVLYY253 pKa = 10.04YY254 pKa = 10.58KK255 pKa = 10.71YY256 pKa = 10.85NYY258 pKa = 9.42YY259 pKa = 10.92LDD261 pKa = 3.49MDD263 pKa = 4.6DD264 pKa = 4.1YY265 pKa = 11.84VRR267 pKa = 11.84EE268 pKa = 4.15KK269 pKa = 11.31GNYY272 pKa = 9.96GYY274 pKa = 10.88DD275 pKa = 3.9LDD277 pKa = 4.8CSSDD281 pKa = 2.87GWSNMQVEE289 pKa = 5.12EE290 pKa = 4.92YY291 pKa = 10.51IFDD294 pKa = 4.38DD295 pKa = 3.94EE296 pKa = 5.72ARR298 pKa = 11.84ITDD301 pKa = 3.76SKK303 pKa = 11.64DD304 pKa = 3.01SGFITYY310 pKa = 9.92YY311 pKa = 10.61KK312 pKa = 9.89YY313 pKa = 11.07DD314 pKa = 3.35SDD316 pKa = 5.26GRR318 pKa = 11.84IEE320 pKa = 5.0DD321 pKa = 3.61DD322 pKa = 4.87SIYY325 pKa = 10.5EE326 pKa = 4.15DD327 pKa = 3.18SSIYY331 pKa = 10.61KK332 pKa = 8.71KK333 pKa = 9.3TFDD336 pKa = 4.13DD337 pKa = 3.86VDD339 pKa = 4.28VNICPYY345 pKa = 10.68SEE347 pKa = 4.0VEE349 pKa = 3.83KK350 pKa = 10.68RR351 pKa = 11.84LEE353 pKa = 3.85EE354 pKa = 3.89RR355 pKa = 11.84RR356 pKa = 11.84EE357 pKa = 3.96EE358 pKa = 3.97IGLSEE363 pKa = 4.81DD364 pKa = 3.75VMGG367 pKa = 5.24

Molecular weight: 42.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H9GMT9|A0A1H9GMT9_9FIRM SpoIIIJ-associated protein OS=Butyrivibrio sp. TB OX=1520809 GN=SAMN02910382_03271 PE=4 SV=1
MM1 pKa = 7.7AKK3 pKa = 8.06MTFQPHH9 pKa = 5.63KK10 pKa = 8.81LQRR13 pKa = 11.84KK14 pKa = 7.68RR15 pKa = 11.84VHH17 pKa = 6.29GFRR20 pKa = 11.84ARR22 pKa = 11.84MSTPGGRR29 pKa = 11.84KK30 pKa = 8.7VLAARR35 pKa = 11.84RR36 pKa = 11.84LKK38 pKa = 10.38GRR40 pKa = 11.84KK41 pKa = 8.94RR42 pKa = 11.84LSAA45 pKa = 3.96

Molecular weight: 5.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3714

0

3714

1257469

39

3300

338.6

38.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.028 ± 0.035

1.375 ± 0.015

6.931 ± 0.039

6.915 ± 0.042

4.28 ± 0.028

6.826 ± 0.036

1.671 ± 0.016

7.988 ± 0.04

6.808 ± 0.036

8.398 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.024 ± 0.023

4.732 ± 0.031

3.063 ± 0.02

2.739 ± 0.021

3.999 ± 0.029

6.619 ± 0.045

5.457 ± 0.038

6.6 ± 0.031

0.909 ± 0.014

4.639 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski