Dolosicoccus paucivorans
Average proteome isoelectric point is 5.93
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1609 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2N6SM31|A0A2N6SM31_9LACT Dihydroorotate dehydrogenase OS=Dolosicoccus paucivorans OX=84521 GN=pyrD PE=3 SV=1
MM1 pKa = 7.42 SVLKK5 pKa = 10.56 KK6 pKa = 10.41 IIYY9 pKa = 9.79 GLLIGWLGAFSLLTSSAQADD29 pKa = 3.97 GVTNIVMTGSDD40 pKa = 3.4 RR41 pKa = 11.84 QADD44 pKa = 3.76 GSGQSDD50 pKa = 3.62 STLVVSYY57 pKa = 11.48 NEE59 pKa = 3.96 GTGQVSLISLPQNLMISIPEE79 pKa = 3.94 QGEE82 pKa = 4.17 GTLAEE87 pKa = 4.78 AYY89 pKa = 10.45 SIGGGQLVADD99 pKa = 5.93 GIEE102 pKa = 3.96 EE103 pKa = 4.2 WTDD106 pKa = 3.29 ASIDD110 pKa = 3.87 DD111 pKa = 4.39 YY112 pKa = 11.31 LTLDD116 pKa = 3.49 MAGVAEE122 pKa = 5.49 LIDD125 pKa = 3.81 EE126 pKa = 4.51 VGGVTVYY133 pKa = 10.59 VSEE136 pKa = 4.7 SFEE139 pKa = 3.72 QDD141 pKa = 2.58 GYY143 pKa = 11.54 SFQEE147 pKa = 4.26 GDD149 pKa = 3.95 TLTMDD154 pKa = 4.31 GSQALAYY161 pKa = 8.43 MRR163 pKa = 11.84 HH164 pKa = 6.06 LPQEE168 pKa = 4.43 GEE170 pKa = 4.08 LVSKK174 pKa = 8.32 QEE176 pKa = 3.78 RR177 pKa = 11.84 QAQVIRR183 pKa = 11.84 SLGEE187 pKa = 3.61 EE188 pKa = 4.08 LAGEE192 pKa = 4.34 DD193 pKa = 4.16 LSAFGYY199 pKa = 10.13 IKK201 pKa = 10.67 LYY203 pKa = 10.48 QNYY206 pKa = 9.64 SGYY209 pKa = 10.06 IDD211 pKa = 3.81 SSLGKK216 pKa = 9.27 MEE218 pKa = 4.88 MINLVKK224 pKa = 10.36 EE225 pKa = 4.73 LIQSGSGLSYY235 pKa = 11.18 HH236 pKa = 6.66 EE237 pKa = 5.7 LDD239 pKa = 3.92 EE240 pKa = 4.45 QTTPDD245 pKa = 3.77 QLQMLLQSS253 pKa = 3.97
Molecular weight: 27.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.719
IPC2_protein 3.834
IPC_protein 3.808
Toseland 3.617
ProMoST 3.948
Dawson 3.783
Bjellqvist 3.935
Wikipedia 3.681
Rodwell 3.643
Grimsley 3.528
Solomon 3.77
Lehninger 3.732
Nozaki 3.897
DTASelect 4.075
Thurlkill 3.656
EMBOSS 3.694
Sillero 3.923
Patrickios 1.1
IPC_peptide 3.77
IPC2_peptide 3.91
IPC2.peptide.svr19 3.823
Protein with the highest isoelectric point:
>tr|A0A2N6SKT2|A0A2N6SKT2_9LACT 60 kDa chaperonin OS=Dolosicoccus paucivorans OX=84521 GN=groL PE=3 SV=1
MM1 pKa = 7.36 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 9.7 QPKK8 pKa = 8.93 KK9 pKa = 9.66 RR10 pKa = 11.84 KK11 pKa = 9.39 RR12 pKa = 11.84 QTTHH16 pKa = 5.48 GFRR19 pKa = 11.84 NRR21 pKa = 11.84 MKK23 pKa = 9.56 TKK25 pKa = 10.26 NGRR28 pKa = 11.84 HH29 pKa = 3.75 VLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 KK37 pKa = 8.81 GRR39 pKa = 11.84 KK40 pKa = 8.91 RR41 pKa = 11.84 LTVV44 pKa = 3.11
Molecular weight: 5.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.451
IPC2_protein 11.169
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.457
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.179
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.066
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1609
0
1609
511702
25
1795
318.0
35.83
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.932 ± 0.061
0.474 ± 0.015
5.888 ± 0.055
7.661 ± 0.077
4.132 ± 0.041
6.422 ± 0.054
2.205 ± 0.026
7.268 ± 0.051
5.864 ± 0.049
10.013 ± 0.075
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.658 ± 0.032
4.341 ± 0.037
3.771 ± 0.039
4.791 ± 0.054
4.189 ± 0.047
5.698 ± 0.043
5.827 ± 0.046
7.069 ± 0.05
0.926 ± 0.02
3.871 ± 0.043
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here