Tortoise microvirus 88

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 7.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4P8WAA5|A0A4P8WAA5_9VIRU Major capsid protein OS=Tortoise microvirus 88 OX=2583196 PE=4 SV=1
MM1 pKa = 7.29ARR3 pKa = 11.84SVTVRR8 pKa = 11.84QLAQSVKK15 pKa = 9.77PMGRR19 pKa = 11.84VMRR22 pKa = 11.84RR23 pKa = 11.84PQHH26 pKa = 5.09TFNIRR31 pKa = 11.84HH32 pKa = 6.23RR33 pKa = 11.84PWQIQPFMIAPVLPGEE49 pKa = 4.29TMKK52 pKa = 11.16NLLLQSRR59 pKa = 11.84VVSDD63 pKa = 3.87PVKK66 pKa = 10.65HH67 pKa = 6.28PLIGWWQEE75 pKa = 3.67YY76 pKa = 8.57YY77 pKa = 10.76FFYY80 pKa = 10.85VKK82 pKa = 10.47HH83 pKa = 6.87RR84 pKa = 11.84DD85 pKa = 3.23LEE87 pKa = 4.35GRR89 pKa = 11.84DD90 pKa = 4.69DD91 pKa = 4.05FTQMMLDD98 pKa = 3.84PQHH101 pKa = 6.49EE102 pKa = 4.61LSSLDD107 pKa = 3.53TPANALTYY115 pKa = 10.43HH116 pKa = 6.97GLTGLDD122 pKa = 3.27WTAMCLEE129 pKa = 4.21RR130 pKa = 11.84VVAEE134 pKa = 3.93YY135 pKa = 10.57FRR137 pKa = 11.84DD138 pKa = 3.95EE139 pKa = 4.16EE140 pKa = 4.33DD141 pKa = 3.34LASVFEE147 pKa = 4.75LGGLPLASISRR158 pKa = 11.84SDD160 pKa = 3.59FAQSLVTATEE170 pKa = 4.44FAARR174 pKa = 11.84QPEE177 pKa = 4.46DD178 pKa = 3.53APADD182 pKa = 3.75ADD184 pKa = 3.54ASGVITASEE193 pKa = 4.11IEE195 pKa = 4.56GALQMWQFQRR205 pKa = 11.84SNGLTKK211 pKa = 9.48MDD213 pKa = 4.25YY214 pKa = 11.13EE215 pKa = 5.01DD216 pKa = 5.16FLATYY221 pKa = 8.79GVNMPKK227 pKa = 10.46EE228 pKa = 4.17EE229 pKa = 4.03LHH231 pKa = 6.14KK232 pKa = 10.74PEE234 pKa = 5.51LIRR237 pKa = 11.84YY238 pKa = 7.71VRR240 pKa = 11.84DD241 pKa = 2.86WTYY244 pKa = 10.62PANTVDD250 pKa = 4.44PSTGTPSSALSWAVAEE266 pKa = 4.68SADD269 pKa = 3.29KK270 pKa = 11.19DD271 pKa = 3.73RR272 pKa = 11.84FFRR275 pKa = 11.84EE276 pKa = 3.6PGFIFGVSVCRR287 pKa = 11.84PKK289 pKa = 10.76VYY291 pKa = 10.6YY292 pKa = 10.43SRR294 pKa = 11.84QTSAAVSMMTDD305 pKa = 3.2AFSWLPAVTSDD316 pKa = 5.69DD317 pKa = 3.49PWTSLKK323 pKa = 10.34PFQAGEE329 pKa = 4.65GPFSGATEE337 pKa = 4.77GYY339 pKa = 9.4WIDD342 pKa = 3.87IKK344 pKa = 11.38DD345 pKa = 3.71LLLYY349 pKa = 10.62GDD351 pKa = 3.64QFINFVPAVGEE362 pKa = 4.07AGFVALPTAGLQRR375 pKa = 11.84KK376 pKa = 7.75FASDD380 pKa = 3.29ADD382 pKa = 3.99ANALFTDD389 pKa = 3.81VAKK392 pKa = 11.03NLIRR396 pKa = 11.84SDD398 pKa = 4.25GIVSLGIAGQQVDD411 pKa = 4.13TTPVV415 pKa = 2.79

Molecular weight: 46.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4P8WA53|A0A4P8WA53_9VIRU Replication initiation protein OS=Tortoise microvirus 88 OX=2583196 PE=4 SV=1
MM1 pKa = 7.27NMSALAKK8 pKa = 9.3GYY10 pKa = 9.47PMPSPVRR17 pKa = 11.84PQGMTGYY24 pKa = 9.75QRR26 pKa = 11.84QPLRR30 pKa = 11.84LPVPANDD37 pKa = 3.77NPRR40 pKa = 11.84PRR42 pKa = 11.84PRR44 pKa = 11.84PANDD48 pKa = 3.01NFRR51 pKa = 11.84PTPRR55 pKa = 11.84LPPAKK60 pKa = 9.18PFSPPRR66 pKa = 11.84VAPKK70 pKa = 10.41GAWLGYY76 pKa = 9.97KK77 pKa = 10.11LAARR81 pKa = 11.84GAARR85 pKa = 11.84FIPYY89 pKa = 10.26LGAALTAFEE98 pKa = 4.54IYY100 pKa = 9.36EE101 pKa = 3.74WWRR104 pKa = 11.84AGEE107 pKa = 4.18VVPEE111 pKa = 4.26VPEE114 pKa = 4.75HH115 pKa = 6.09IALNGWRR122 pKa = 11.84HH123 pKa = 5.73VGHH126 pKa = 6.71CPKK129 pKa = 10.88GGGGWTHH136 pKa = 7.17RR137 pKa = 11.84GWGDD141 pKa = 3.44QPPTQTQINQANSCLPGQGLSVWLPWGSPYY171 pKa = 10.02PANSRR176 pKa = 11.84TLIAFKK182 pKa = 8.99EE183 pKa = 4.19THH185 pKa = 7.03RR186 pKa = 11.84IGTTPYY192 pKa = 9.68GEE194 pKa = 4.02VRR196 pKa = 11.84EE197 pKa = 4.37VFSRR201 pKa = 11.84PVGGTVTWPEE211 pKa = 3.98YY212 pKa = 10.59VPYY215 pKa = 10.44SPAVLPDD222 pKa = 3.46VVPSPRR228 pKa = 11.84SVPEE232 pKa = 3.45VHH234 pKa = 6.93PGIDD238 pKa = 3.65PFSVPPLAPVAPPAPLPVRR257 pKa = 11.84ALPYY261 pKa = 9.95RR262 pKa = 11.84VSNPMRR268 pKa = 11.84AEE270 pKa = 3.6QSVRR274 pKa = 11.84GFGVNPRR281 pKa = 11.84SVPRR285 pKa = 11.84VNPRR289 pKa = 11.84RR290 pKa = 11.84RR291 pKa = 11.84PPGVRR296 pKa = 11.84EE297 pKa = 3.95RR298 pKa = 11.84KK299 pKa = 9.35FKK301 pKa = 10.28LTISGSIVSNAFSFVTEE318 pKa = 4.1GADD321 pKa = 3.3VVEE324 pKa = 5.61AIYY327 pKa = 10.14EE328 pKa = 4.52AIPEE332 pKa = 4.52SIRR335 pKa = 11.84GTRR338 pKa = 11.84KK339 pKa = 10.1RR340 pKa = 11.84GVKK343 pKa = 8.7EE344 pKa = 3.15QAYY347 pKa = 8.6FIWQHH352 pKa = 5.98LSQVDD357 pKa = 3.8QNVMVQNLLYY367 pKa = 11.0NHH369 pKa = 6.71FEE371 pKa = 4.43DD372 pKa = 5.09KK373 pKa = 10.89FFGAIGEE380 pKa = 4.26RR381 pKa = 11.84MKK383 pKa = 10.74RR384 pKa = 11.84ANQVVWEE391 pKa = 4.04QGYY394 pKa = 11.02GNLRR398 pKa = 11.84VQFGLAHH405 pKa = 6.79

Molecular weight: 45.0 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1344

78

415

268.8

29.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.226 ± 0.576

1.19 ± 0.631

4.464 ± 1.155

5.283 ± 0.301

4.092 ± 0.592

7.738 ± 0.592

2.083 ± 0.309

3.274 ± 0.436

4.092 ± 0.784

7.813 ± 0.939

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.307 ± 0.314

3.125 ± 0.507

8.185 ± 2.006

3.869 ± 0.494

8.78 ± 1.395

6.696 ± 0.738

4.911 ± 0.63

7.887 ± 0.555

2.009 ± 0.367

2.976 ± 0.32
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski