Natranaerobius thermophilus (strain ATCC BAA-1301 / DSM 18059 / JW/NM-WN-LF)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Natranaerobiales; Natranaerobiaceae; Natranaerobius; Natranaerobius thermophilus

Average proteome isoelectric point is 5.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2847 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B2A357|B2A357_NATTJ Flagellar protein FliL OS=Natranaerobius thermophilus (strain ATCC BAA-1301 / DSM 18059 / JW/NM-WN-LF) OX=457570 GN=Nther_1404 PE=3 SV=1
MM1 pKa = 7.81RR2 pKa = 11.84KK3 pKa = 9.49LLILLLVVFVASFGFMGCDD22 pKa = 2.96PADD25 pKa = 3.76PEE27 pKa = 4.4EE28 pKa = 4.2IAEE31 pKa = 4.25EE32 pKa = 4.29EE33 pKa = 4.17EE34 pKa = 4.38KK35 pKa = 11.04EE36 pKa = 4.19PEE38 pKa = 3.84EE39 pKa = 4.89DD40 pKa = 3.13VDD42 pKa = 4.05EE43 pKa = 4.76EE44 pKa = 4.53EE45 pKa = 5.08EE46 pKa = 4.12EE47 pKa = 4.29LEE49 pKa = 4.5QAITHH54 pKa = 6.98AIWSEE59 pKa = 3.53PDD61 pKa = 3.12GLFNYY66 pKa = 10.15CEE68 pKa = 4.09YY69 pKa = 10.96EE70 pKa = 3.94STYY73 pKa = 11.51DD74 pKa = 4.12LDD76 pKa = 4.34AFNQVFDD83 pKa = 4.4GMMEE87 pKa = 4.72ADD89 pKa = 3.44PHH91 pKa = 7.34ADD93 pKa = 4.11LEE95 pKa = 4.72LNPNLAEE102 pKa = 3.94EE103 pKa = 4.91HH104 pKa = 6.85EE105 pKa = 4.37ISDD108 pKa = 4.21DD109 pKa = 3.9GKK111 pKa = 8.59TFYY114 pKa = 11.2YY115 pKa = 10.65KK116 pKa = 10.69LRR118 pKa = 11.84DD119 pKa = 4.44DD120 pKa = 4.85IEE122 pKa = 4.4FHH124 pKa = 7.39DD125 pKa = 5.0GEE127 pKa = 4.86PLTAEE132 pKa = 3.91DD133 pKa = 4.33VKK135 pKa = 10.85FTFEE139 pKa = 4.15WMCHH143 pKa = 5.0EE144 pKa = 5.54DD145 pKa = 3.89YY146 pKa = 10.84IGPRR150 pKa = 11.84ASYY153 pKa = 8.11WQHH156 pKa = 7.44LEE158 pKa = 3.87GFDD161 pKa = 3.55EE162 pKa = 4.36YY163 pKa = 11.17RR164 pKa = 11.84AGEE167 pKa = 3.98ADD169 pKa = 3.56EE170 pKa = 4.7VEE172 pKa = 4.74GIEE175 pKa = 5.03IISDD179 pKa = 3.6HH180 pKa = 6.55EE181 pKa = 4.27IEE183 pKa = 4.25FHH185 pKa = 6.88FEE187 pKa = 3.87QVDD190 pKa = 3.67ASAVYY195 pKa = 10.16YY196 pKa = 10.6VSTWGISPKK205 pKa = 10.37HH206 pKa = 5.04VWEE209 pKa = 5.49DD210 pKa = 3.3IPVGEE215 pKa = 4.13RR216 pKa = 11.84RR217 pKa = 11.84EE218 pKa = 4.15APEE221 pKa = 3.59MTEE224 pKa = 4.42PIGTGPFEE232 pKa = 3.97FEE234 pKa = 4.52EE235 pKa = 4.51YY236 pKa = 11.08VEE238 pKa = 4.17GQYY241 pKa = 10.73VEE243 pKa = 5.52LVANEE248 pKa = 4.34DD249 pKa = 3.59YY250 pKa = 11.14HH251 pKa = 8.68RR252 pKa = 11.84GEE254 pKa = 4.68PEE256 pKa = 3.78LEE258 pKa = 4.55RR259 pKa = 11.84ITVEE263 pKa = 4.04VKK265 pKa = 10.77SPDD268 pKa = 3.4VVRR271 pKa = 11.84ADD273 pKa = 3.95LEE275 pKa = 4.3TGEE278 pKa = 4.23VDD280 pKa = 3.24IAEE283 pKa = 4.34VPPDD287 pKa = 3.34EE288 pKa = 5.64SEE290 pKa = 3.69WDD292 pKa = 3.57DD293 pKa = 4.32YY294 pKa = 11.34EE295 pKa = 4.8AHH297 pKa = 7.52DD298 pKa = 4.33EE299 pKa = 4.28LALEE303 pKa = 4.33YY304 pKa = 10.67YY305 pKa = 7.33PTNGYY310 pKa = 10.6QYY312 pKa = 10.33MGMNLRR318 pKa = 11.84DD319 pKa = 3.36EE320 pKa = 5.71SIFSDD325 pKa = 3.48HH326 pKa = 6.85AVRR329 pKa = 11.84EE330 pKa = 4.21AATYY334 pKa = 9.89AINRR338 pKa = 11.84EE339 pKa = 3.87GMVDD343 pKa = 4.46GILDD347 pKa = 3.76GLGKK351 pKa = 10.02VQNSHH356 pKa = 7.31FSPNQWAYY364 pKa = 10.99DD365 pKa = 4.13EE366 pKa = 5.96DD367 pKa = 5.88LDD369 pKa = 4.92TYY371 pKa = 11.15PHH373 pKa = 7.17DD374 pKa = 4.3PDD376 pKa = 3.43KK377 pKa = 11.64AEE379 pKa = 5.25EE380 pKa = 3.99ILEE383 pKa = 4.32DD384 pKa = 4.34AGYY387 pKa = 8.44TKK389 pKa = 10.9NDD391 pKa = 3.6DD392 pKa = 5.28GIWEE396 pKa = 4.24QNGEE400 pKa = 4.15PLEE403 pKa = 4.12FTLLYY408 pKa = 8.0PTGDD412 pKa = 3.56EE413 pKa = 4.17PRR415 pKa = 11.84EE416 pKa = 3.74QAALIIEE423 pKa = 4.12QDD425 pKa = 3.61LQGIGIDD432 pKa = 3.24ITLEE436 pKa = 3.93SLEE439 pKa = 4.3FATLSDD445 pKa = 3.55RR446 pKa = 11.84VFDD449 pKa = 5.33EE450 pKa = 4.35IDD452 pKa = 3.08FDD454 pKa = 4.68AYY456 pKa = 11.44LMGWSLGADD465 pKa = 3.81PDD467 pKa = 3.94PTGIWGPDD475 pKa = 2.91EE476 pKa = 4.44RR477 pKa = 11.84FNAVGFVHH485 pKa = 6.94PEE487 pKa = 3.55SDD489 pKa = 3.1EE490 pKa = 4.8LMEE493 pKa = 4.34QGLRR497 pKa = 11.84TTDD500 pKa = 3.09RR501 pKa = 11.84DD502 pKa = 3.35EE503 pKa = 4.6RR504 pKa = 11.84RR505 pKa = 11.84EE506 pKa = 4.4HH507 pKa = 5.55YY508 pKa = 10.85VEE510 pKa = 3.92WQRR513 pKa = 11.84LLQEE517 pKa = 3.79EE518 pKa = 4.5MPYY521 pKa = 10.61VFLYY525 pKa = 10.89ADD527 pKa = 3.77YY528 pKa = 11.15EE529 pKa = 4.81GYY531 pKa = 10.93AYY533 pKa = 11.19NNDD536 pKa = 2.95IQVFEE541 pKa = 4.6PNPWNIWYY549 pKa = 8.82DD550 pKa = 3.29VHH552 pKa = 6.54EE553 pKa = 5.02WYY555 pKa = 11.05LEE557 pKa = 3.89

Molecular weight: 64.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B2A498|B2A498_NATTJ Putative rRNA methylase OS=Natranaerobius thermophilus (strain ATCC BAA-1301 / DSM 18059 / JW/NM-WN-LF) OX=457570 GN=Nther_0153 PE=4 SV=1
MM1 pKa = 7.36KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 9.67QPKK8 pKa = 9.0KK9 pKa = 7.87RR10 pKa = 11.84QRR12 pKa = 11.84KK13 pKa = 8.39KK14 pKa = 8.49EE15 pKa = 3.5HH16 pKa = 6.11GFRR19 pKa = 11.84KK20 pKa = 9.77RR21 pKa = 11.84MKK23 pKa = 9.23TKK25 pKa = 10.17AGRR28 pKa = 11.84NILRR32 pKa = 11.84NRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.18GRR39 pKa = 11.84KK40 pKa = 6.43TLSAA44 pKa = 4.15

Molecular weight: 5.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2847

0

2847

893166

31

1474

313.7

35.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.063 ± 0.048

0.884 ± 0.019

5.848 ± 0.042

8.555 ± 0.068

4.126 ± 0.037

6.95 ± 0.047

1.837 ± 0.018

8.064 ± 0.049

6.829 ± 0.057

9.808 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.472 ± 0.021

4.969 ± 0.035

3.642 ± 0.022

3.827 ± 0.036

3.953 ± 0.03

6.028 ± 0.033

5.086 ± 0.028

6.671 ± 0.036

0.817 ± 0.016

3.573 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski