Amino acid dipepetide frequency for Natranaerobius thermophilus (strain ATCC BAA-1301 / DSM 18059 / JW/NM-WN-LF)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.162AlaAla: 4.162 ± 0.088
0.69AlaCys: 0.69 ± 0.031
3.066AlaAsp: 3.066 ± 0.066
4.514AlaGlu: 4.514 ± 0.09
2.508AlaPhe: 2.508 ± 0.061
4.796AlaGly: 4.796 ± 0.085
1.068AlaHis: 1.068 ± 0.036
5.121AlaIle: 5.121 ± 0.08
3.969AlaLys: 3.969 ± 0.07
6.414AlaLeu: 6.414 ± 0.114
1.63AlaMet: 1.63 ± 0.045
2.479AlaAsn: 2.479 ± 0.057
1.843AlaPro: 1.843 ± 0.046
1.964AlaGln: 1.964 ± 0.049
2.73AlaArg: 2.73 ± 0.061
3.637AlaSer: 3.637 ± 0.072
3.002AlaThr: 3.002 ± 0.057
4.636AlaVal: 4.636 ± 0.071
0.446AlaTrp: 0.446 ± 0.022
1.954AlaTyr: 1.954 ± 0.046
0.0AlaXaa: 0.0 ± 0.0
Cys
0.442CysAla: 0.442 ± 0.022
0.094CysCys: 0.094 ± 0.01
0.54CysAsp: 0.54 ± 0.026
0.636CysGlu: 0.636 ± 0.031
0.343CysPhe: 0.343 ± 0.02
0.879CysGly: 0.879 ± 0.041
0.271CysHis: 0.271 ± 0.02
0.574CysIle: 0.574 ± 0.028
0.506CysLys: 0.506 ± 0.026
0.696CysLeu: 0.696 ± 0.026
0.188CysMet: 0.188 ± 0.013
0.408CysAsn: 0.408 ± 0.024
0.545CysPro: 0.545 ± 0.031
0.43CysGln: 0.43 ± 0.025
0.373CysArg: 0.373 ± 0.021
0.599CysSer: 0.599 ± 0.029
0.394CysThr: 0.394 ± 0.02
0.502CysVal: 0.502 ± 0.028
0.079CysTrp: 0.079 ± 0.01
0.34CysTyr: 0.34 ± 0.02
0.0CysXaa: 0.0 ± 0.0
Asp
2.768AspAla: 2.768 ± 0.066
0.532AspCys: 0.532 ± 0.03
3.219AspAsp: 3.219 ± 0.077
5.463AspGlu: 5.463 ± 0.097
2.581AspPhe: 2.581 ± 0.053
3.488AspGly: 3.488 ± 0.074
1.142AspHis: 1.142 ± 0.038
4.988AspIle: 4.988 ± 0.083
4.375AspLys: 4.375 ± 0.064
5.983AspLeu: 5.983 ± 0.083
1.537AspMet: 1.537 ± 0.036
3.033AspAsn: 3.033 ± 0.061
2.263AspPro: 2.263 ± 0.06
2.277AspGln: 2.277 ± 0.051
2.443AspArg: 2.443 ± 0.054
3.317AspSer: 3.317 ± 0.061
2.573AspThr: 2.573 ± 0.058
3.301AspVal: 3.301 ± 0.061
0.55AspTrp: 0.55 ± 0.024
2.648AspTyr: 2.648 ± 0.061
0.0AspXaa: 0.0 ± 0.0
Glu
4.925GluAla: 4.925 ± 0.091
0.478GluCys: 0.478 ± 0.027
5.271GluAsp: 5.271 ± 0.119
9.067GluGlu: 9.067 ± 0.176
3.1GluPhe: 3.1 ± 0.058
4.86GluGly: 4.86 ± 0.081
1.498GluHis: 1.498 ± 0.044
7.359GluIle: 7.359 ± 0.115
6.372GluLys: 6.372 ± 0.103
9.18GluLeu: 9.18 ± 0.116
2.216GluMet: 2.216 ± 0.059
4.659GluAsn: 4.659 ± 0.077
2.365GluPro: 2.365 ± 0.059
4.049GluGln: 4.049 ± 0.081
3.263GluArg: 3.263 ± 0.068
3.951GluSer: 3.951 ± 0.07
4.027GluThr: 4.027 ± 0.081
5.442GluVal: 5.442 ± 0.099
0.637GluTrp: 0.637 ± 0.029
2.833GluTyr: 2.833 ± 0.068
0.0GluXaa: 0.0 ± 0.0
Phe
2.347PheAla: 2.347 ± 0.06
0.402PheCys: 0.402 ± 0.022
2.437PheAsp: 2.437 ± 0.059
2.875PheGlu: 2.875 ± 0.056
1.878PhePhe: 1.878 ± 0.054
2.924PheGly: 2.924 ± 0.075
0.785PheHis: 0.785 ± 0.031
3.323PheIle: 3.323 ± 0.063
2.357PheLys: 2.357 ± 0.055
4.068PheLeu: 4.068 ± 0.083
1.023PheMet: 1.023 ± 0.036
2.091PheAsn: 2.091 ± 0.049
1.637PhePro: 1.637 ± 0.051
1.59PheGln: 1.59 ± 0.037
1.447PheArg: 1.447 ± 0.04
3.05PheSer: 3.05 ± 0.063
2.201PheThr: 2.201 ± 0.052
2.678PheVal: 2.678 ± 0.067
0.448PheTrp: 0.448 ± 0.028
1.703PheTyr: 1.703 ± 0.048
0.0PheXaa: 0.0 ± 0.0
Gly
4.65GlyAla: 4.65 ± 0.088
0.859GlyCys: 0.859 ± 0.04
3.61GlyAsp: 3.61 ± 0.067
5.671GlyGlu: 5.671 ± 0.094
3.133GlyPhe: 3.133 ± 0.067
4.986GlyGly: 4.986 ± 0.096
1.303GlyHis: 1.303 ± 0.04
6.215GlyIle: 6.215 ± 0.097
4.673GlyLys: 4.673 ± 0.083
6.678GlyLeu: 6.678 ± 0.111
1.928GlyMet: 1.928 ± 0.053
2.78GlyAsn: 2.78 ± 0.058
1.863GlyPro: 1.863 ± 0.041
2.304GlyGln: 2.304 ± 0.054
2.671GlyArg: 2.671 ± 0.057
3.774GlySer: 3.774 ± 0.074
3.894GlyThr: 3.894 ± 0.066
5.088GlyVal: 5.088 ± 0.096
0.555GlyTrp: 0.555 ± 0.026
2.861GlyTyr: 2.861 ± 0.059
0.0GlyXaa: 0.0 ± 0.0
His
0.947HisAla: 0.947 ± 0.032
0.19HisCys: 0.19 ± 0.015
1.118HisAsp: 1.118 ± 0.038
1.413HisGlu: 1.413 ± 0.039
0.808HisPhe: 0.808 ± 0.029
1.49HisGly: 1.49 ± 0.039
0.508HisHis: 0.508 ± 0.026
1.311HisIle: 1.311 ± 0.036
1.153HisLys: 1.153 ± 0.037
1.787HisLeu: 1.787 ± 0.04
0.376HisMet: 0.376 ± 0.022
0.939HisAsn: 0.939 ± 0.037
1.002HisPro: 1.002 ± 0.037
0.718HisGln: 0.718 ± 0.033
0.749HisArg: 0.749 ± 0.029
1.117HisSer: 1.117 ± 0.038
0.769HisThr: 0.769 ± 0.03
1.079HisVal: 1.079 ± 0.038
0.169HisTrp: 0.169 ± 0.013
0.728HisTyr: 0.728 ± 0.03
0.0HisXaa: 0.0 ± 0.0
Ile
5.347IleAla: 5.347 ± 0.089
0.692IleCys: 0.692 ± 0.029
5.072IleAsp: 5.072 ± 0.084
6.152IleGlu: 6.152 ± 0.083
3.469IlePhe: 3.469 ± 0.069
5.356IleGly: 5.356 ± 0.085
1.342IleHis: 1.342 ± 0.042
6.574IleIle: 6.574 ± 0.109
5.519IleLys: 5.519 ± 0.091
7.59IleLeu: 7.59 ± 0.12
1.879IleMet: 1.879 ± 0.05
4.379IleAsn: 4.379 ± 0.066
3.377IlePro: 3.377 ± 0.066
2.365IleGln: 2.365 ± 0.049
2.763IleArg: 2.763 ± 0.05
5.824IleSer: 5.824 ± 0.079
4.623IleThr: 4.623 ± 0.085
5.127IleVal: 5.127 ± 0.081
0.592IleTrp: 0.592 ± 0.027
2.6IleTyr: 2.6 ± 0.057
0.0IleXaa: 0.0 ± 0.0
Lys
4.077LysAla: 4.077 ± 0.069
0.508LysCys: 0.508 ± 0.029
4.23LysAsp: 4.23 ± 0.076
6.82LysGlu: 6.82 ± 0.102
2.273LysPhe: 2.273 ± 0.052
4.117LysGly: 4.117 ± 0.076
1.182LysHis: 1.182 ± 0.042
5.446LysIle: 5.446 ± 0.09
5.262LysLys: 5.262 ± 0.092
6.478LysLeu: 6.478 ± 0.11
1.684LysMet: 1.684 ± 0.041
3.881LysAsn: 3.881 ± 0.074
2.051LysPro: 2.051 ± 0.046
2.917LysGln: 2.917 ± 0.07
2.627LysArg: 2.627 ± 0.066
3.718LysSer: 3.718 ± 0.063
3.554LysThr: 3.554 ± 0.064
4.501LysVal: 4.501 ± 0.076
0.503LysTrp: 0.503 ± 0.026
2.462LysTyr: 2.462 ± 0.059
0.0LysXaa: 0.0 ± 0.0
Leu
6.771LeuAla: 6.771 ± 0.099
0.705LeuCys: 0.705 ± 0.031
5.781LeuAsp: 5.781 ± 0.081
8.13LeuGlu: 8.13 ± 0.123
4.026LeuPhe: 4.026 ± 0.085
7.066LeuGly: 7.066 ± 0.125
1.533LeuHis: 1.533 ± 0.04
8.016LeuIle: 8.016 ± 0.124
6.935LeuLys: 6.935 ± 0.099
9.099LeuLeu: 9.099 ± 0.146
2.224LeuMet: 2.224 ± 0.054
4.839LeuAsn: 4.839 ± 0.071
3.866LeuPro: 3.866 ± 0.072
3.284LeuGln: 3.284 ± 0.063
3.65LeuArg: 3.65 ± 0.071
6.694LeuSer: 6.694 ± 0.102
5.5LeuThr: 5.5 ± 0.082
6.33LeuVal: 6.33 ± 0.098
0.666LeuTrp: 0.666 ± 0.027
2.97LeuTyr: 2.97 ± 0.051
0.0LeuXaa: 0.0 ± 0.0
Met
1.976MetAla: 1.976 ± 0.05
0.169MetCys: 0.169 ± 0.014
1.441MetAsp: 1.441 ± 0.037
2.118MetGlu: 2.118 ± 0.046
0.815MetPhe: 0.815 ± 0.03
2.016MetGly: 2.016 ± 0.051
0.298MetHis: 0.298 ± 0.017
1.939MetIle: 1.939 ± 0.046
1.728MetLys: 1.728 ± 0.041
2.132MetLeu: 2.132 ± 0.048
0.626MetMet: 0.626 ± 0.031
1.254MetAsn: 1.254 ± 0.035
0.826MetPro: 0.826 ± 0.029
0.712MetGln: 0.712 ± 0.03
0.932MetArg: 0.932 ± 0.031
1.638MetSer: 1.638 ± 0.04
1.453MetThr: 1.453 ± 0.041
1.824MetVal: 1.824 ± 0.05
0.151MetTrp: 0.151 ± 0.012
0.671MetTyr: 0.671 ± 0.027
0.0MetXaa: 0.0 ± 0.0
Asn
2.137AsnAla: 2.137 ± 0.051
0.59AsnCys: 0.59 ± 0.028
2.405AsnAsp: 2.405 ± 0.057
3.498AsnGlu: 3.498 ± 0.07
2.293AsnPhe: 2.293 ± 0.052
2.472AsnGly: 2.472 ± 0.056
1.003AsnHis: 1.003 ± 0.032
4.11AsnIle: 4.11 ± 0.077
3.717AsnLys: 3.717 ± 0.076
5.366AsnLeu: 5.366 ± 0.079
1.322AsnMet: 1.322 ± 0.039
2.952AsnAsn: 2.952 ± 0.054
2.184AsnPro: 2.184 ± 0.056
2.316AsnGln: 2.316 ± 0.055
2.208AsnArg: 2.208 ± 0.048
3.116AsnSer: 3.116 ± 0.059
2.355AsnThr: 2.355 ± 0.051
2.833AsnVal: 2.833 ± 0.061
0.562AsnTrp: 0.562 ± 0.022
2.244AsnTyr: 2.244 ± 0.058
0.0AsnXaa: 0.0 ± 0.0
Pro
1.953ProAla: 1.953 ± 0.044
0.322ProCys: 0.322 ± 0.018
2.333ProAsp: 2.333 ± 0.051
3.829ProGlu: 3.829 ± 0.076
1.598ProPhe: 1.598 ± 0.047
3.03ProGly: 3.03 ± 0.072
0.814ProHis: 0.814 ± 0.032
2.524ProIle: 2.524 ± 0.06
2.119ProLys: 2.119 ± 0.054
3.298ProLeu: 3.298 ± 0.069
0.756ProMet: 0.756 ± 0.029
1.473ProAsn: 1.473 ± 0.039
1.249ProPro: 1.249 ± 0.045
1.391ProGln: 1.391 ± 0.037
1.414ProArg: 1.414 ± 0.042
1.964ProSer: 1.964 ± 0.047
1.685ProThr: 1.685 ± 0.045
2.951ProVal: 2.951 ± 0.06
0.334ProTrp: 0.334 ± 0.02
1.383ProTyr: 1.383 ± 0.038
0.0ProXaa: 0.0 ± 0.0
Gln
2.462GlnAla: 2.462 ± 0.051
0.237GlnCys: 0.237 ± 0.018
2.554GlnAsp: 2.554 ± 0.058
4.242GlnGlu: 4.242 ± 0.079
1.202GlnPhe: 1.202 ± 0.037
2.879GlnGly: 2.879 ± 0.068
0.575GlnHis: 0.575 ± 0.029
2.903GlnIle: 2.903 ± 0.06
2.885GlnLys: 2.885 ± 0.055
3.678GlnLeu: 3.678 ± 0.077
0.85GlnMet: 0.85 ± 0.03
1.987GlnAsn: 1.987 ± 0.046
0.979GlnPro: 0.979 ± 0.031
1.575GlnGln: 1.575 ± 0.076
1.477GlnArg: 1.477 ± 0.044
1.855GlnSer: 1.855 ± 0.043
1.601GlnThr: 1.601 ± 0.039
2.995GlnVal: 2.995 ± 0.059
0.264GlnTrp: 0.264 ± 0.017
1.065GlnTyr: 1.065 ± 0.032
0.0GlnXaa: 0.0 ± 0.0
Arg
2.403ArgAla: 2.403 ± 0.058
0.357ArgCys: 0.357 ± 0.021
2.339ArgAsp: 2.339 ± 0.052
3.884ArgGlu: 3.884 ± 0.081
1.527ArgPhe: 1.527 ± 0.045
2.717ArgGly: 2.717 ± 0.052
0.738ArgHis: 0.738 ± 0.034
3.034ArgIle: 3.034 ± 0.061
2.815ArgLys: 2.815 ± 0.055
3.667ArgLeu: 3.667 ± 0.075
0.968ArgMet: 0.968 ± 0.033
1.874ArgAsn: 1.874 ± 0.054
1.383ArgPro: 1.383 ± 0.038
1.612ArgGln: 1.612 ± 0.05
1.797ArgArg: 1.797 ± 0.042
2.015ArgSer: 2.015 ± 0.052
1.85ArgThr: 1.85 ± 0.043
2.8ArgVal: 2.8 ± 0.063
0.327ArgTrp: 0.327 ± 0.02
1.422ArgTyr: 1.422 ± 0.036
0.0ArgXaa: 0.0 ± 0.0
Ser
3.249SerAla: 3.249 ± 0.061
0.561SerCys: 0.561 ± 0.027
3.32SerAsp: 3.32 ± 0.059
4.754SerGlu: 4.754 ± 0.071
2.628SerPhe: 2.628 ± 0.063
4.53SerGly: 4.53 ± 0.084
1.266SerHis: 1.266 ± 0.044
4.365SerIle: 4.365 ± 0.076
3.99SerLys: 3.99 ± 0.068
6.207SerLeu: 6.207 ± 0.086
1.551SerMet: 1.551 ± 0.042
2.731SerAsn: 2.731 ± 0.056
2.403SerPro: 2.403 ± 0.051
2.945SerGln: 2.945 ± 0.061
2.555SerArg: 2.555 ± 0.059
3.867SerSer: 3.867 ± 0.082
2.864SerThr: 2.864 ± 0.063
3.653SerVal: 3.653 ± 0.069
0.583SerTrp: 0.583 ± 0.026
2.261SerTyr: 2.261 ± 0.049
0.0SerXaa: 0.0 ± 0.0
Thr
3.462ThrAla: 3.462 ± 0.063
0.431ThrCys: 0.431 ± 0.022
2.892ThrAsp: 2.892 ± 0.056
4.041ThrGlu: 4.041 ± 0.09
2.097ThrPhe: 2.097 ± 0.051
4.468ThrGly: 4.468 ± 0.078
0.921ThrHis: 0.921 ± 0.031
3.932ThrIle: 3.932 ± 0.068
3.082ThrLys: 3.082 ± 0.053
4.636ThrLeu: 4.636 ± 0.072
1.236ThrMet: 1.236 ± 0.034
2.304ThrAsn: 2.304 ± 0.05
2.203ThrPro: 2.203 ± 0.054
1.597ThrGln: 1.597 ± 0.045
1.973ThrArg: 1.973 ± 0.045
2.993ThrSer: 2.993 ± 0.066
2.712ThrThr: 2.712 ± 0.05
3.866ThrVal: 3.866 ± 0.077
0.449ThrTrp: 0.449 ± 0.02
1.561ThrTyr: 1.561 ± 0.041
0.0ThrXaa: 0.0 ± 0.0
Val
4.408ValAla: 4.408 ± 0.081
0.545ValCys: 0.545 ± 0.027
4.196ValAsp: 4.196 ± 0.075
5.242ValGlu: 5.242 ± 0.094
2.815ValPhe: 2.815 ± 0.062
4.56ValGly: 4.56 ± 0.087
1.048ValHis: 1.048 ± 0.037
5.829ValIle: 5.829 ± 0.088
4.332ValLys: 4.332 ± 0.075
6.244ValLeu: 6.244 ± 0.093
1.775ValMet: 1.775 ± 0.043
3.411ValAsn: 3.411 ± 0.058
2.565ValPro: 2.565 ± 0.049
1.804ValGln: 1.804 ± 0.045
2.355ValArg: 2.355 ± 0.05
4.539ValSer: 4.539 ± 0.083
3.816ValThr: 3.816 ± 0.075
4.739ValVal: 4.739 ± 0.087
0.397ValTrp: 0.397 ± 0.024
2.087ValTyr: 2.087 ± 0.055
0.0ValXaa: 0.0 ± 0.0
Trp
0.481TrpAla: 0.481 ± 0.024
0.065TrpCys: 0.065 ± 0.008
0.572TrpAsp: 0.572 ± 0.03
0.784TrpGlu: 0.784 ± 0.029
0.368TrpPhe: 0.368 ± 0.022
0.595TrpGly: 0.595 ± 0.029
0.171TrpHis: 0.171 ± 0.015
0.605TrpIle: 0.605 ± 0.03
0.506TrpLys: 0.506 ± 0.021
0.832TrpLeu: 0.832 ± 0.032
0.195TrpMet: 0.195 ± 0.016
0.401TrpAsn: 0.401 ± 0.021
0.278TrpPro: 0.278 ± 0.019
0.329TrpGln: 0.329 ± 0.017
0.334TrpArg: 0.334 ± 0.019
0.381TrpSer: 0.381 ± 0.022
0.343TrpThr: 0.343 ± 0.019
0.522TrpVal: 0.522 ± 0.026
0.113TrpTrp: 0.113 ± 0.012
0.293TrpTyr: 0.293 ± 0.019
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.66TyrAla: 1.66 ± 0.047
0.411TyrCys: 0.411 ± 0.024
2.085TyrAsp: 2.085 ± 0.054
2.419TyrGlu: 2.419 ± 0.054
1.803TyrPhe: 1.803 ± 0.044
2.266TyrGly: 2.266 ± 0.056
0.906TyrHis: 0.906 ± 0.033
2.472TyrIle: 2.472 ± 0.053
1.993TyrLys: 1.993 ± 0.055
4.051TyrLeu: 4.051 ± 0.069
0.755TyrMet: 0.755 ± 0.029
1.809TyrAsn: 1.809 ± 0.044
1.538TyrPro: 1.538 ± 0.038
2.089TyrGln: 2.089 ± 0.057
1.771TyrArg: 1.771 ± 0.045
2.233TyrSer: 2.233 ± 0.054
1.642TyrThr: 1.642 ± 0.044
1.84TyrVal: 1.84 ± 0.044
0.34TyrTrp: 0.34 ± 0.017
1.642TyrTyr: 1.642 ± 0.047
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2847 proteins (893166 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski