Ferrimonas balearica (strain DSM 9799 / CCM 4581 / KCTC 23876 / PAT)
Average proteome isoelectric point is 6.06
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3781 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|E1SU05|E1SU05_FERBD Uncharacterized protein OS=Ferrimonas balearica (strain DSM 9799 / CCM 4581 / KCTC 23876 / PAT) OX=550540 GN=Fbal_3049 PE=4 SV=1
MM1 pKa = 8.32 RR2 pKa = 11.84 YY3 pKa = 9.02 PLLASAVLLALTGCGSGSDD22 pKa = 3.68 SDD24 pKa = 4.55 SGNTPDD30 pKa = 4.85 PTPQPTPNTAPTLSLSQYY48 pKa = 6.97 TTAEE52 pKa = 4.08 DD53 pKa = 4.28 TPFSLSLADD62 pKa = 4.53 LVSDD66 pKa = 3.87 VDD68 pKa = 3.8 GDD70 pKa = 4.2 NISLSVEE77 pKa = 3.75 ACGDD81 pKa = 3.49 QLSCQLSGTTLTLTPADD98 pKa = 4.92 DD99 pKa = 3.75 YY100 pKa = 11.56 HH101 pKa = 8.5 GEE103 pKa = 3.93 QSVRR107 pKa = 11.84 LSASDD112 pKa = 4.29 GEE114 pKa = 4.45 ASTSAEE120 pKa = 3.75 LTLTVEE126 pKa = 4.71 AVNDD130 pKa = 3.86 APLLAVSDD138 pKa = 3.91 QQVEE142 pKa = 4.23 EE143 pKa = 4.96 DD144 pKa = 4.14 GSLTLDD150 pKa = 3.7 LASWISDD157 pKa = 3.19 VDD159 pKa = 3.64 GDD161 pKa = 4.58 ALTLSVASCAANIDD175 pKa = 3.86 CALDD179 pKa = 3.7 NTSLTLTPAADD190 pKa = 3.55 HH191 pKa = 6.79 FGDD194 pKa = 3.62 SHH196 pKa = 8.75 SIGLTVTDD204 pKa = 4.35 AANASTSASFNLTVSGVNDD223 pKa = 3.63 APQWQAIAAQTLQYY237 pKa = 10.54 DD238 pKa = 3.5 QSLTLDD244 pKa = 4.24 LTRR247 pKa = 11.84 HH248 pKa = 5.87 IADD251 pKa = 4.48 ADD253 pKa = 3.76 NDD255 pKa = 4.18 SLSFSVVSCDD265 pKa = 4.72 NLQCQLNGSEE275 pKa = 4.63 LTLSAPAEE283 pKa = 4.27 AGVTYY288 pKa = 10.24 PLTLRR293 pKa = 11.84 ATDD296 pKa = 4.05 GSDD299 pKa = 3.61 EE300 pKa = 4.3 VDD302 pKa = 3.06 TALQVAITKK311 pKa = 9.9 VPVDD315 pKa = 4.97 AIAGVQFFPPYY326 pKa = 10.33 NGLTGVDD333 pKa = 3.61 NVTLSLHH340 pKa = 6.07 SEE342 pKa = 3.85 QDD344 pKa = 3.45 LTQLTINGEE353 pKa = 4.46 VITLEE358 pKa = 5.28 NPRR361 pKa = 11.84 QWQQNFPLQMGDD373 pKa = 3.37 TLFTVVLEE381 pKa = 4.5 TATEE385 pKa = 4.17 TLSLTKK391 pKa = 9.46 TVRR394 pKa = 11.84 YY395 pKa = 9.9 QGDD398 pKa = 3.76 LHH400 pKa = 7.13 QGSADD405 pKa = 3.41 LTYY408 pKa = 11.0 LGDD411 pKa = 3.64 RR412 pKa = 11.84 TVLVDD417 pKa = 4.4 DD418 pKa = 4.28 YY419 pKa = 11.59 RR420 pKa = 11.84 AGALVAFDD428 pKa = 4.46 LDD430 pKa = 3.92 TRR432 pKa = 11.84 EE433 pKa = 4.04 TTAYY437 pKa = 9.84 PSDD440 pKa = 3.95 RR441 pKa = 11.84 LTGMNIDD448 pKa = 3.6 FLDD451 pKa = 3.72 WYY453 pKa = 10.52 VALDD457 pKa = 3.55 SEE459 pKa = 4.72 RR460 pKa = 11.84 LLGSAWDD467 pKa = 3.63 QDD469 pKa = 3.34 AAANKK474 pKa = 9.62 LVEE477 pKa = 4.41 VNLTDD482 pKa = 3.41 LTITEE487 pKa = 5.09 RR488 pKa = 11.84 YY489 pKa = 8.88 NLSNVPVQVSHH500 pKa = 6.49 LTASTDD506 pKa = 2.9 ASLVYY511 pKa = 9.42 FVFDD515 pKa = 5.15 NSRR518 pKa = 11.84 QDD520 pKa = 3.62 PNTGAWYY527 pKa = 8.77 PVEE530 pKa = 4.31 EE531 pKa = 4.93 FYY533 pKa = 11.15 QFEE536 pKa = 4.53 VATNTLTLLADD547 pKa = 4.12 TAQVDD552 pKa = 3.84 DD553 pKa = 5.56 DD554 pKa = 4.68 GPLTRR559 pKa = 11.84 VRR561 pKa = 11.84 GMILDD566 pKa = 3.83 EE567 pKa = 4.5 TNDD570 pKa = 3.48 RR571 pKa = 11.84 LLMVNQNYY579 pKa = 10.1 SDD581 pKa = 3.7 PAARR585 pKa = 11.84 LLALPLSGPDD595 pKa = 3.02 EE596 pKa = 4.42 GRR598 pKa = 11.84 LVEE601 pKa = 4.64 LTPSSDD607 pKa = 3.46 PGCLDD612 pKa = 3.98 FAAQEE617 pKa = 4.26 YY618 pKa = 10.16 YY619 pKa = 10.53 EE620 pKa = 4.38 LRR622 pKa = 11.84 NVRR625 pKa = 11.84 DD626 pKa = 4.38 GKK628 pKa = 10.13 WLMVMQNDD636 pKa = 3.15 NWASYY641 pKa = 10.6 QLDD644 pKa = 3.83 INTLCLTEE652 pKa = 3.78 HH653 pKa = 6.36 HH654 pKa = 6.69 RR655 pKa = 11.84 VPEE658 pKa = 4.02 SLLLNRR664 pKa = 11.84 EE665 pKa = 4.13 SLQGIDD671 pKa = 3.68 VNPLTGEE678 pKa = 4.06 ILLGFYY684 pKa = 9.57 PGPIVYY690 pKa = 9.92 QPEE693 pKa = 3.87 TDD695 pKa = 3.21 SYY697 pKa = 11.26 RR698 pKa = 11.84 GAEE701 pKa = 3.82 AAGFAGEE708 pKa = 4.15 PWNIQEE714 pKa = 4.38 PTAIHH719 pKa = 5.85 YY720 pKa = 9.45 RR721 pKa = 11.84 ADD723 pKa = 3.18 THH725 pKa = 6.55 QIVYY729 pKa = 10.79 GDD731 pKa = 4.37 DD732 pKa = 3.39 QFLMQLDD739 pKa = 4.32 LQTGEE744 pKa = 4.31 VTQLAQLRR752 pKa = 11.84 DD753 pKa = 4.13 NILKK757 pKa = 10.62 LEE759 pKa = 4.04 WDD761 pKa = 3.93 EE762 pKa = 4.51 ASQRR766 pKa = 11.84 YY767 pKa = 8.28 YY768 pKa = 11.07 LYY770 pKa = 10.77 DD771 pKa = 3.76 EE772 pKa = 4.62 YY773 pKa = 11.43 DD774 pKa = 3.21 GLLAYY779 pKa = 10.07 DD780 pKa = 3.58 VAGEE784 pKa = 3.92 QLVTLVEE791 pKa = 4.31 EE792 pKa = 4.64 ASGQHH797 pKa = 5.83 FSYY800 pKa = 10.27 PALASDD806 pKa = 3.63 GQLYY810 pKa = 10.01 YY811 pKa = 10.8 VDD813 pKa = 4.91 SDD815 pKa = 3.64 VLNRR819 pKa = 11.84 INPEE823 pKa = 3.72 TLEE826 pKa = 4.16 INTVSPHH833 pKa = 6.84 DD834 pKa = 4.11 DD835 pKa = 3.13 AAGFRR840 pKa = 11.84 SITSLHH846 pKa = 6.55 LDD848 pKa = 3.54 EE849 pKa = 5.69 PNNRR853 pKa = 11.84 AIISVSSGGDD863 pKa = 3.08 TTYY866 pKa = 10.75 IGLDD870 pKa = 3.24 LDD872 pKa = 3.88 SGVRR876 pKa = 11.84 TILLPEE882 pKa = 4.51 SDD884 pKa = 5.34 LNDD887 pKa = 3.22 GDD889 pKa = 4.58 YY890 pKa = 11.35 NILSADD896 pKa = 4.02 GSLMWYY902 pKa = 9.99 FDD904 pKa = 3.6 WANDD908 pKa = 3.5 QVMSLNLDD916 pKa = 3.59 TQAITPVTPASTNHH930 pKa = 5.78 YY931 pKa = 10.33 KK932 pKa = 10.87 LGDD935 pKa = 3.49 PTGLFRR941 pKa = 11.84 LSDD944 pKa = 5.27 DD945 pKa = 3.66 ILLMGDD951 pKa = 3.78 DD952 pKa = 4.49 DD953 pKa = 6.52 FGGFWMINLTTDD965 pKa = 2.78 EE966 pKa = 4.32 KK967 pKa = 11.28 LLYY970 pKa = 10.19 RR971 pKa = 4.69
Molecular weight: 105.69 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.689
IPC2_protein 3.719
IPC_protein 3.77
Toseland 3.528
ProMoST 3.935
Dawson 3.77
Bjellqvist 3.923
Wikipedia 3.706
Rodwell 3.592
Grimsley 3.439
Solomon 3.77
Lehninger 3.719
Nozaki 3.872
DTASelect 4.151
Thurlkill 3.579
EMBOSS 3.719
Sillero 3.897
Patrickios 0.96
IPC_peptide 3.757
IPC2_peptide 3.872
IPC2.peptide.svr19 3.793
Protein with the highest isoelectric point:
>tr|E1SRV5|E1SRV5_FERBD DNA mismatch repair protein MutH OS=Ferrimonas balearica (strain DSM 9799 / CCM 4581 / KCTC 23876 / PAT) OX=550540 GN=mutH PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.46 RR12 pKa = 11.84 KK13 pKa = 9.37 RR14 pKa = 11.84 NHH16 pKa = 5.37 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.4 NGRR28 pKa = 11.84 QVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.39 GRR39 pKa = 11.84 ARR41 pKa = 11.84 LSAA44 pKa = 3.91
Molecular weight: 5.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.676
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.398
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.177
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3781
0
3781
1264585
30
3058
334.5
36.78
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.712 ± 0.059
1.051 ± 0.016
5.671 ± 0.037
6.01 ± 0.039
3.54 ± 0.025
7.826 ± 0.035
2.378 ± 0.021
4.624 ± 0.029
3.377 ± 0.033
11.46 ± 0.067
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.596 ± 0.019
3.202 ± 0.027
4.78 ± 0.03
4.942 ± 0.038
5.934 ± 0.035
5.591 ± 0.031
5.035 ± 0.027
7.067 ± 0.036
1.446 ± 0.016
2.757 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here