Streptococcus phage Javan366

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 50 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6B0D2|A0A4D6B0D2_9CAUD Portal (Connector) protein OS=Streptococcus phage Javan366 OX=2548127 GN=Javan366_0030 PE=4 SV=1
MM1 pKa = 7.65IIKK4 pKa = 9.96NYY6 pKa = 10.16KK7 pKa = 8.43YY8 pKa = 9.83DD9 pKa = 3.64YY10 pKa = 9.83SGGRR14 pKa = 11.84IFYY17 pKa = 9.63TIDD20 pKa = 2.93VDD22 pKa = 5.15GYY24 pKa = 8.18EE25 pKa = 3.94QTMEE29 pKa = 4.1HH30 pKa = 6.41TKK32 pKa = 10.0TEE34 pKa = 4.21YY35 pKa = 11.24GSVQRR40 pKa = 11.84NDD42 pKa = 2.91IDD44 pKa = 4.87DD45 pKa = 4.0FLGTVEE51 pKa = 5.34EE52 pKa = 4.61YY53 pKa = 10.98DD54 pKa = 3.57FQEE57 pKa = 4.79AEE59 pKa = 4.03IIEE62 pKa = 4.37AFVDD66 pKa = 4.35FQNDD70 pKa = 3.31LLLYY74 pKa = 10.76GIDD77 pKa = 3.5FEE79 pKa = 5.44LRR81 pKa = 11.84NEE83 pKa = 4.21VEE85 pKa = 4.03

Molecular weight: 10.13 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6B047|A0A4D6B047_9CAUD Uncharacterized protein OS=Streptococcus phage Javan366 OX=2548127 GN=Javan366_0042 PE=4 SV=1
MM1 pKa = 7.74FILKK5 pKa = 10.18HH6 pKa = 4.74GTKK9 pKa = 10.06EE10 pKa = 3.89EE11 pKa = 4.04KK12 pKa = 9.97PYY14 pKa = 11.29LMSAKK19 pKa = 10.03IGVTGIDD26 pKa = 2.8ISFSEE31 pKa = 4.21EE32 pKa = 3.18RR33 pKa = 11.84GAIRR37 pKa = 11.84FVSRR41 pKa = 11.84AVAMQVAKK49 pKa = 10.71ALRR52 pKa = 11.84SFGNFYY58 pKa = 10.66VIQVKK63 pKa = 10.07GG64 pKa = 3.29

Molecular weight: 7.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

50

0

50

8941

39

1108

178.8

20.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.677 ± 0.534

0.582 ± 0.136

6.375 ± 0.36

8.232 ± 0.604

4.149 ± 0.284

6.174 ± 0.386

1.443 ± 0.194

7.124 ± 0.335

8.332 ± 0.378

8.746 ± 0.358

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.595 ± 0.24

5.626 ± 0.272

2.64 ± 0.209

4.25 ± 0.306

4.239 ± 0.372

5.861 ± 0.304

6.174 ± 0.355

5.805 ± 0.331

1.264 ± 0.201

3.713 ± 0.317
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski