Amino acid dipepetide frequency for Streptococcus phage Javan366

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.356AlaAla: 3.356 ± 0.847
0.447AlaCys: 0.447 ± 0.25
4.698AlaAsp: 4.698 ± 0.641
5.593AlaGlu: 5.593 ± 0.853
2.125AlaPhe: 2.125 ± 0.435
4.027AlaGly: 4.027 ± 0.655
0.895AlaHis: 0.895 ± 0.322
5.593AlaIle: 5.593 ± 1.166
4.474AlaLys: 4.474 ± 0.611
7.718AlaLeu: 7.718 ± 1.219
2.573AlaMet: 2.573 ± 0.439
3.579AlaAsn: 3.579 ± 0.8
1.566AlaPro: 1.566 ± 0.347
2.796AlaGln: 2.796 ± 0.678
2.796AlaArg: 2.796 ± 0.596
3.132AlaSer: 3.132 ± 0.667
3.803AlaThr: 3.803 ± 0.831
3.691AlaVal: 3.691 ± 0.631
0.671AlaTrp: 0.671 ± 0.314
3.244AlaTyr: 3.244 ± 0.623
0.0AlaXaa: 0.0 ± 0.0
Cys
0.112CysAla: 0.112 ± 0.108
0.0CysCys: 0.0 ± 0.0
0.336CysAsp: 0.336 ± 0.211
0.224CysGlu: 0.224 ± 0.16
0.224CysPhe: 0.224 ± 0.141
0.224CysGly: 0.224 ± 0.148
0.336CysHis: 0.336 ± 0.203
0.559CysIle: 0.559 ± 0.299
0.447CysLys: 0.447 ± 0.234
0.783CysLeu: 0.783 ± 0.315
0.112CysMet: 0.112 ± 0.095
0.447CysAsn: 0.447 ± 0.222
0.224CysPro: 0.224 ± 0.147
0.447CysGln: 0.447 ± 0.221
0.224CysArg: 0.224 ± 0.148
0.224CysSer: 0.224 ± 0.157
0.336CysThr: 0.336 ± 0.264
0.112CysVal: 0.112 ± 0.115
0.0CysTrp: 0.0 ± 0.0
0.447CysTyr: 0.447 ± 0.269
0.0CysXaa: 0.0 ± 0.0
Asp
3.579AspAla: 3.579 ± 0.7
0.447AspCys: 0.447 ± 0.208
3.02AspAsp: 3.02 ± 0.796
5.593AspGlu: 5.593 ± 0.983
3.356AspPhe: 3.356 ± 0.682
4.922AspGly: 4.922 ± 0.909
1.007AspHis: 1.007 ± 0.332
6.376AspIle: 6.376 ± 0.898
5.034AspLys: 5.034 ± 0.671
4.139AspLeu: 4.139 ± 0.685
1.678AspMet: 1.678 ± 0.387
3.356AspAsn: 3.356 ± 0.776
2.125AspPro: 2.125 ± 0.528
1.342AspGln: 1.342 ± 0.404
3.02AspArg: 3.02 ± 0.687
4.251AspSer: 4.251 ± 0.642
3.02AspThr: 3.02 ± 0.519
3.244AspVal: 3.244 ± 0.56
1.119AspTrp: 1.119 ± 0.258
3.132AspTyr: 3.132 ± 0.675
0.0AspXaa: 0.0 ± 0.0
Glu
5.369GluAla: 5.369 ± 0.948
0.224GluCys: 0.224 ± 0.149
3.579GluAsp: 3.579 ± 0.696
7.047GluGlu: 7.047 ± 1.031
3.356GluPhe: 3.356 ± 0.703
4.586GluGly: 4.586 ± 0.776
0.783GluHis: 0.783 ± 0.259
5.928GluIle: 5.928 ± 0.911
6.488GluLys: 6.488 ± 1.063
10.403GluLeu: 10.403 ± 1.142
3.02GluMet: 3.02 ± 0.762
4.251GluAsn: 4.251 ± 0.553
1.79GluPro: 1.79 ± 0.659
5.145GluGln: 5.145 ± 0.974
3.02GluArg: 3.02 ± 0.581
3.356GluSer: 3.356 ± 0.688
4.922GluThr: 4.922 ± 1.029
5.369GluVal: 5.369 ± 0.778
1.119GluTrp: 1.119 ± 0.353
2.573GluTyr: 2.573 ± 0.576
0.0GluXaa: 0.0 ± 0.0
Phe
2.461PheAla: 2.461 ± 0.518
0.224PheCys: 0.224 ± 0.158
2.573PheAsp: 2.573 ± 0.719
3.579PheGlu: 3.579 ± 0.771
1.79PhePhe: 1.79 ± 0.538
3.132PheGly: 3.132 ± 0.687
0.447PheHis: 0.447 ± 0.226
3.356PheIle: 3.356 ± 0.64
4.027PheLys: 4.027 ± 0.636
2.685PheLeu: 2.685 ± 0.564
1.454PheMet: 1.454 ± 0.331
3.244PheAsn: 3.244 ± 0.742
1.119PhePro: 1.119 ± 0.433
1.566PheGln: 1.566 ± 0.459
1.342PheArg: 1.342 ± 0.29
1.902PheSer: 1.902 ± 0.463
2.013PheThr: 2.013 ± 0.393
2.685PheVal: 2.685 ± 0.665
0.224PheTrp: 0.224 ± 0.168
1.678PheTyr: 1.678 ± 0.384
0.0PheXaa: 0.0 ± 0.0
Gly
3.915GlyAla: 3.915 ± 1.189
0.336GlyCys: 0.336 ± 0.202
5.257GlyAsp: 5.257 ± 0.711
3.579GlyGlu: 3.579 ± 0.553
2.685GlyPhe: 2.685 ± 0.553
4.362GlyGly: 4.362 ± 0.714
0.447GlyHis: 0.447 ± 0.176
4.474GlyIle: 4.474 ± 0.643
5.145GlyLys: 5.145 ± 0.728
5.257GlyLeu: 5.257 ± 0.781
1.342GlyMet: 1.342 ± 0.361
3.468GlyAsn: 3.468 ± 0.732
0.895GlyPro: 0.895 ± 0.288
1.902GlyGln: 1.902 ± 0.537
2.349GlyArg: 2.349 ± 0.465
4.027GlySer: 4.027 ± 0.79
3.691GlyThr: 3.691 ± 0.644
3.803GlyVal: 3.803 ± 0.477
1.454GlyTrp: 1.454 ± 0.636
3.356GlyTyr: 3.356 ± 0.554
0.0GlyXaa: 0.0 ± 0.0
His
0.447HisAla: 0.447 ± 0.223
0.0HisCys: 0.0 ± 0.0
0.895HisAsp: 0.895 ± 0.388
0.671HisGlu: 0.671 ± 0.293
1.007HisPhe: 1.007 ± 0.241
0.671HisGly: 0.671 ± 0.256
0.224HisHis: 0.224 ± 0.163
1.566HisIle: 1.566 ± 0.399
1.454HisLys: 1.454 ± 0.385
1.23HisLeu: 1.23 ± 0.381
0.671HisMet: 0.671 ± 0.263
0.895HisAsn: 0.895 ± 0.372
0.336HisPro: 0.336 ± 0.225
0.559HisGln: 0.559 ± 0.269
0.559HisArg: 0.559 ± 0.231
0.783HisSer: 0.783 ± 0.282
1.119HisThr: 1.119 ± 0.304
0.783HisVal: 0.783 ± 0.336
0.0HisTrp: 0.0 ± 0.0
0.559HisTyr: 0.559 ± 0.429
0.0HisXaa: 0.0 ± 0.0
Ile
6.152IleAla: 6.152 ± 0.691
0.447IleCys: 0.447 ± 0.269
5.481IleAsp: 5.481 ± 0.876
4.362IleGlu: 4.362 ± 0.554
2.796IlePhe: 2.796 ± 0.603
4.139IleGly: 4.139 ± 0.756
1.119IleHis: 1.119 ± 0.319
4.922IleIle: 4.922 ± 0.83
7.494IleLys: 7.494 ± 0.909
5.593IleLeu: 5.593 ± 0.805
1.454IleMet: 1.454 ± 0.514
3.579IleAsn: 3.579 ± 0.693
1.566IlePro: 1.566 ± 0.39
3.803IleGln: 3.803 ± 0.616
3.244IleArg: 3.244 ± 0.588
5.034IleSer: 5.034 ± 0.91
4.474IleThr: 4.474 ± 0.628
3.132IleVal: 3.132 ± 0.713
0.224IleTrp: 0.224 ± 0.142
2.237IleTyr: 2.237 ± 0.52
0.0IleXaa: 0.0 ± 0.0
Lys
6.488LysAla: 6.488 ± 1.025
0.224LysCys: 0.224 ± 0.144
5.928LysAsp: 5.928 ± 0.906
8.389LysGlu: 8.389 ± 1.192
3.244LysPhe: 3.244 ± 0.674
3.915LysGly: 3.915 ± 0.702
1.902LysHis: 1.902 ± 0.454
4.362LysIle: 4.362 ± 0.741
7.271LysLys: 7.271 ± 1.115
6.488LysLeu: 6.488 ± 0.701
1.902LysMet: 1.902 ± 0.482
5.369LysAsn: 5.369 ± 0.617
2.796LysPro: 2.796 ± 0.54
3.132LysGln: 3.132 ± 0.712
3.915LysArg: 3.915 ± 0.708
4.586LysSer: 4.586 ± 0.643
6.04LysThr: 6.04 ± 0.728
3.356LysVal: 3.356 ± 0.654
1.23LysTrp: 1.23 ± 0.323
2.796LysTyr: 2.796 ± 0.443
0.0LysXaa: 0.0 ± 0.0
Leu
6.264LeuAla: 6.264 ± 0.863
0.447LeuCys: 0.447 ± 0.232
6.04LeuAsp: 6.04 ± 0.648
8.054LeuGlu: 8.054 ± 1.31
3.132LeuPhe: 3.132 ± 0.708
6.376LeuGly: 6.376 ± 0.788
1.566LeuHis: 1.566 ± 0.411
4.922LeuIle: 4.922 ± 0.972
9.396LeuLys: 9.396 ± 0.898
6.488LeuLeu: 6.488 ± 1.065
2.349LeuMet: 2.349 ± 0.679
4.698LeuAsn: 4.698 ± 0.882
2.573LeuPro: 2.573 ± 0.619
2.573LeuGln: 2.573 ± 0.654
4.362LeuArg: 4.362 ± 0.629
5.705LeuSer: 5.705 ± 0.76
5.481LeuThr: 5.481 ± 0.795
3.468LeuVal: 3.468 ± 0.544
0.895LeuTrp: 0.895 ± 0.291
2.685LeuTyr: 2.685 ± 0.528
0.0LeuXaa: 0.0 ± 0.0
Met
2.349MetAla: 2.349 ± 0.563
0.0MetCys: 0.0 ± 0.0
1.454MetAsp: 1.454 ± 0.411
1.79MetGlu: 1.79 ± 0.448
1.23MetPhe: 1.23 ± 0.35
0.895MetGly: 0.895 ± 0.249
0.112MetHis: 0.112 ± 0.132
2.013MetIle: 2.013 ± 0.577
1.902MetLys: 1.902 ± 0.487
1.454MetLeu: 1.454 ± 0.347
0.783MetMet: 0.783 ± 0.263
2.013MetAsn: 2.013 ± 0.472
0.671MetPro: 0.671 ± 0.273
1.342MetGln: 1.342 ± 0.29
1.23MetArg: 1.23 ± 0.409
2.685MetSer: 2.685 ± 0.593
1.902MetThr: 1.902 ± 0.42
1.566MetVal: 1.566 ± 0.342
0.112MetTrp: 0.112 ± 0.099
0.447MetTyr: 0.447 ± 0.266
0.0MetXaa: 0.0 ± 0.0
Asn
3.132AsnAla: 3.132 ± 0.699
0.224AsnCys: 0.224 ± 0.157
3.244AsnAsp: 3.244 ± 0.631
5.257AsnGlu: 5.257 ± 0.567
2.237AsnPhe: 2.237 ± 0.391
4.922AsnGly: 4.922 ± 0.94
1.23AsnHis: 1.23 ± 0.401
3.02AsnIle: 3.02 ± 0.553
3.915AsnLys: 3.915 ± 0.594
5.034AsnLeu: 5.034 ± 0.745
1.007AsnMet: 1.007 ± 0.387
2.685AsnAsn: 2.685 ± 0.563
2.573AsnPro: 2.573 ± 0.664
3.915AsnGln: 3.915 ± 0.733
1.79AsnArg: 1.79 ± 0.381
3.356AsnSer: 3.356 ± 0.81
2.908AsnThr: 2.908 ± 0.417
4.251AsnVal: 4.251 ± 0.728
0.336AsnTrp: 0.336 ± 0.197
1.23AsnTyr: 1.23 ± 0.574
0.0AsnXaa: 0.0 ± 0.0
Pro
1.902ProAla: 1.902 ± 0.539
0.224ProCys: 0.224 ± 0.165
1.79ProAsp: 1.79 ± 0.769
1.902ProGlu: 1.902 ± 0.529
0.783ProPhe: 0.783 ± 0.366
1.342ProGly: 1.342 ± 0.351
0.671ProHis: 0.671 ± 0.246
2.573ProIle: 2.573 ± 0.55
1.454ProLys: 1.454 ± 0.45
2.349ProLeu: 2.349 ± 0.409
0.224ProMet: 0.224 ± 0.145
1.007ProAsn: 1.007 ± 0.316
0.112ProPro: 0.112 ± 0.12
1.79ProGln: 1.79 ± 0.358
0.783ProArg: 0.783 ± 0.268
1.678ProSer: 1.678 ± 0.463
2.573ProThr: 2.573 ± 0.469
1.902ProVal: 1.902 ± 0.439
0.336ProTrp: 0.336 ± 0.225
1.007ProTyr: 1.007 ± 0.389
0.0ProXaa: 0.0 ± 0.0
Gln
4.922GlnAla: 4.922 ± 0.926
0.224GlnCys: 0.224 ± 0.139
1.007GlnAsp: 1.007 ± 0.334
5.034GlnGlu: 5.034 ± 0.783
2.013GlnPhe: 2.013 ± 0.408
1.007GlnGly: 1.007 ± 0.424
0.336GlnHis: 0.336 ± 0.17
2.573GlnIle: 2.573 ± 0.539
2.796GlnLys: 2.796 ± 0.703
4.139GlnLeu: 4.139 ± 0.708
1.119GlnMet: 1.119 ± 0.434
2.908GlnAsn: 2.908 ± 0.491
1.342GlnPro: 1.342 ± 0.327
1.678GlnGln: 1.678 ± 0.504
2.013GlnArg: 2.013 ± 0.571
2.685GlnSer: 2.685 ± 0.521
2.796GlnThr: 2.796 ± 0.483
2.461GlnVal: 2.461 ± 0.38
0.447GlnTrp: 0.447 ± 0.23
0.895GlnTyr: 0.895 ± 0.286
0.0GlnXaa: 0.0 ± 0.0
Arg
2.796ArgAla: 2.796 ± 0.582
0.336ArgCys: 0.336 ± 0.208
2.013ArgAsp: 2.013 ± 0.663
2.796ArgGlu: 2.796 ± 0.676
1.342ArgPhe: 1.342 ± 0.395
2.573ArgGly: 2.573 ± 0.597
0.336ArgHis: 0.336 ± 0.206
3.803ArgIle: 3.803 ± 0.688
3.803ArgLys: 3.803 ± 1.064
3.579ArgLeu: 3.579 ± 0.763
1.119ArgMet: 1.119 ± 0.356
3.02ArgAsn: 3.02 ± 0.581
1.119ArgPro: 1.119 ± 0.322
1.23ArgGln: 1.23 ± 0.437
2.461ArgArg: 2.461 ± 0.67
2.349ArgSer: 2.349 ± 0.457
2.237ArgThr: 2.237 ± 0.586
2.349ArgVal: 2.349 ± 0.642
1.007ArgTrp: 1.007 ± 0.327
2.125ArgTyr: 2.125 ± 0.644
0.0ArgXaa: 0.0 ± 0.0
Ser
3.244SerAla: 3.244 ± 0.94
0.447SerCys: 0.447 ± 0.212
4.81SerAsp: 4.81 ± 0.848
4.586SerGlu: 4.586 ± 0.955
3.579SerPhe: 3.579 ± 0.497
4.027SerGly: 4.027 ± 0.682
0.671SerHis: 0.671 ± 0.252
3.356SerIle: 3.356 ± 0.528
4.698SerLys: 4.698 ± 0.748
5.257SerLeu: 5.257 ± 0.832
1.119SerMet: 1.119 ± 0.421
3.579SerAsn: 3.579 ± 0.615
1.566SerPro: 1.566 ± 0.368
2.349SerGln: 2.349 ± 0.613
2.461SerArg: 2.461 ± 0.437
3.244SerSer: 3.244 ± 0.526
3.468SerThr: 3.468 ± 0.806
3.356SerVal: 3.356 ± 0.532
0.559SerTrp: 0.559 ± 0.207
2.237SerTyr: 2.237 ± 0.549
0.0SerXaa: 0.0 ± 0.0
Thr
4.698ThrAla: 4.698 ± 0.812
0.336ThrCys: 0.336 ± 0.186
4.027ThrAsp: 4.027 ± 0.628
4.139ThrGlu: 4.139 ± 0.57
2.125ThrPhe: 2.125 ± 0.481
4.251ThrGly: 4.251 ± 0.598
1.23ThrHis: 1.23 ± 0.304
4.586ThrIle: 4.586 ± 0.699
4.698ThrLys: 4.698 ± 0.527
4.922ThrLeu: 4.922 ± 0.721
1.23ThrMet: 1.23 ± 0.402
3.244ThrAsn: 3.244 ± 0.414
0.783ThrPro: 0.783 ± 0.286
2.685ThrGln: 2.685 ± 0.506
2.349ThrArg: 2.349 ± 0.561
3.356ThrSer: 3.356 ± 0.44
4.027ThrThr: 4.027 ± 0.667
5.481ThrVal: 5.481 ± 1.165
1.23ThrTrp: 1.23 ± 0.375
2.349ThrTyr: 2.349 ± 0.632
0.0ThrXaa: 0.0 ± 0.0
Val
3.244ValAla: 3.244 ± 0.548
0.336ValCys: 0.336 ± 0.204
5.034ValAsp: 5.034 ± 0.675
5.145ValGlu: 5.145 ± 0.801
1.678ValPhe: 1.678 ± 0.349
3.579ValGly: 3.579 ± 0.472
0.671ValHis: 0.671 ± 0.232
3.803ValIle: 3.803 ± 0.613
5.257ValLys: 5.257 ± 0.899
5.145ValLeu: 5.145 ± 0.746
1.678ValMet: 1.678 ± 0.338
2.685ValAsn: 2.685 ± 0.603
1.454ValPro: 1.454 ± 0.477
2.349ValGln: 2.349 ± 0.529
1.566ValArg: 1.566 ± 0.359
3.691ValSer: 3.691 ± 0.767
4.474ValThr: 4.474 ± 0.657
4.139ValVal: 4.139 ± 0.792
0.783ValTrp: 0.783 ± 0.458
1.342ValTyr: 1.342 ± 0.415
0.0ValXaa: 0.0 ± 0.0
Trp
0.336TrpAla: 0.336 ± 0.206
0.112TrpCys: 0.112 ± 0.124
0.447TrpAsp: 0.447 ± 0.249
1.119TrpGlu: 1.119 ± 0.298
1.007TrpPhe: 1.007 ± 0.393
0.447TrpGly: 0.447 ± 0.188
0.0TrpHis: 0.0 ± 0.0
1.007TrpIle: 1.007 ± 0.377
1.007TrpLys: 1.007 ± 0.322
1.119TrpLeu: 1.119 ± 0.382
0.224TrpMet: 0.224 ± 0.16
1.007TrpAsn: 1.007 ± 0.302
0.112TrpPro: 0.112 ± 0.096
0.671TrpGln: 0.671 ± 0.211
0.671TrpArg: 0.671 ± 0.235
0.671TrpSer: 0.671 ± 0.25
0.671TrpThr: 0.671 ± 0.244
0.783TrpVal: 0.783 ± 0.283
0.336TrpTrp: 0.336 ± 0.232
0.895TrpTyr: 0.895 ± 0.447
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.013TyrAla: 2.013 ± 0.436
0.783TyrCys: 0.783 ± 0.325
2.125TyrAsp: 2.125 ± 0.534
3.468TyrGlu: 3.468 ± 0.686
1.79TyrPhe: 1.79 ± 0.467
2.349TyrGly: 2.349 ± 0.5
0.447TyrHis: 0.447 ± 0.176
2.461TyrIle: 2.461 ± 0.654
2.573TyrLys: 2.573 ± 0.507
3.691TyrLeu: 3.691 ± 0.661
0.783TyrMet: 0.783 ± 0.279
1.23TyrAsn: 1.23 ± 0.445
1.678TyrPro: 1.678 ± 0.361
1.23TyrGln: 1.23 ± 0.396
2.237TyrArg: 2.237 ± 0.472
1.902TyrSer: 1.902 ± 0.407
1.79TyrThr: 1.79 ± 0.435
2.125TyrVal: 2.125 ± 0.497
0.559TyrTrp: 0.559 ± 0.225
1.902TyrTyr: 1.902 ± 0.592
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 50 proteins (8941 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski