Roseivivax lentus
Average proteome isoelectric point is 6.08
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4153 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1N7P730|A0A1N7P730_9RHOB Initiator Replication protein OS=Roseivivax lentus OX=633194 GN=SAMN05421759_11341 PE=4 SV=1
MM1 pKa = 7.59 ISVTTRR7 pKa = 11.84 AQLEE11 pKa = 4.14 NALASAQAGDD21 pKa = 4.64 EE22 pKa = 4.21 IVLAAGDD29 pKa = 3.82 YY30 pKa = 11.36 GSFDD34 pKa = 4.04 FNGYY38 pKa = 9.77 NYY40 pKa = 10.44 SGYY43 pKa = 8.76 VTVRR47 pKa = 11.84 SADD50 pKa = 3.49 PDD52 pKa = 3.57 NPAVFDD58 pKa = 3.88 QIDD61 pKa = 3.49 IVGSSFLAIDD71 pKa = 3.92 SVHH74 pKa = 6.58 VDD76 pKa = 3.29 NPSNGAASSKK86 pKa = 10.15 VVNIDD91 pKa = 3.56 GNSHH95 pKa = 7.43 DD96 pKa = 3.85 IVFSNSEE103 pKa = 4.09 VNGSVTTSTNYY114 pKa = 10.49 NEE116 pKa = 4.36 FQGHH120 pKa = 4.8 YY121 pKa = 10.51 GIYY124 pKa = 9.64 TGGNVRR130 pKa = 11.84 NIRR133 pKa = 11.84 IEE135 pKa = 4.01 EE136 pKa = 4.2 NEE138 pKa = 3.92 VHH140 pKa = 6.74 DD141 pKa = 4.31 VKK143 pKa = 11.34 NGFVALGSDD152 pKa = 3.76 NIEE155 pKa = 3.89 VVGNHH160 pKa = 6.51 FDD162 pKa = 3.83 RR163 pKa = 11.84 LGNDD167 pKa = 3.09 TMKK170 pKa = 10.56 FAGVNGVLIEE180 pKa = 4.16 NNIGPTSNFPSPTAHH195 pKa = 6.39 VDD197 pKa = 4.61 FIQFQGSSEE206 pKa = 4.3 NITIRR211 pKa = 11.84 GNVSLAGNIGTTQAIFMADD230 pKa = 2.88 GSYY233 pKa = 11.29 NNVLIEE239 pKa = 4.13 QNILYY244 pKa = 9.45 TGMLHH249 pKa = 7.96 GITLYY254 pKa = 10.83 SGANVTIRR262 pKa = 11.84 DD263 pKa = 3.94 NTVLNAPDD271 pKa = 4.37 LVHH274 pKa = 6.97 KK275 pKa = 9.77 ATVIQSPDD283 pKa = 3.09 GATVTGNIISATGYY297 pKa = 9.99 DD298 pKa = 3.75 GKK300 pKa = 11.42 VSGGNLYY307 pKa = 9.69 VQHH310 pKa = 6.82 DD311 pKa = 4.25 QPNQAHH317 pKa = 6.86 HH318 pKa = 6.35 YY319 pKa = 10.64 SDD321 pKa = 4.15 YY322 pKa = 10.88 FPNAEE327 pKa = 4.11 TGLGVTLQDD336 pKa = 4.12 LVPLAGSLAEE346 pKa = 3.99 QYY348 pKa = 10.58 GAYY351 pKa = 8.96 EE352 pKa = 4.04 RR353 pKa = 11.84 LMEE356 pKa = 5.02 LLNGDD361 pKa = 3.5 TWVPEE366 pKa = 4.29 PAPQPDD372 pKa = 5.01 PDD374 pKa = 4.52 PQPEE378 pKa = 4.68 PDD380 pKa = 4.23 PQPLPDD386 pKa = 5.06 LDD388 pKa = 4.05 PAEE391 pKa = 4.33 GTVFSLPGEE400 pKa = 4.12 LTIADD405 pKa = 3.9 AGDD408 pKa = 3.95 VFEE411 pKa = 4.87 MAHH414 pKa = 5.55 SAEE417 pKa = 4.62 LEE419 pKa = 4.09 ISSGTVAFSFTADD432 pKa = 3.53 DD433 pKa = 3.78 VAGVQGLVAKK443 pKa = 10.14 DD444 pKa = 3.1 ASGYY448 pKa = 10.24 VGGGHH453 pKa = 6.98 HH454 pKa = 6.67 FAAYY458 pKa = 10.61 LEE460 pKa = 4.99 GNTLMVRR467 pKa = 11.84 LQDD470 pKa = 3.93 AASDD474 pKa = 4.2 AYY476 pKa = 11.13 LSLDD480 pKa = 3.95 GIEE483 pKa = 5.43 AGTTYY488 pKa = 11.07 DD489 pKa = 3.29 VAVTFGPDD497 pKa = 3.84 GGEE500 pKa = 3.45 LWVNGEE506 pKa = 4.19 QVSTTEE512 pKa = 5.77 LVMDD516 pKa = 4.07 WTQNQQHH523 pKa = 5.91 LQWGGLGWGSADD535 pKa = 3.39 QAAGYY540 pKa = 8.54 DD541 pKa = 3.47 ANFRR545 pKa = 11.84 GTISDD550 pKa = 3.32 ATIYY554 pKa = 8.08 DD555 pKa = 3.86 TKK557 pKa = 10.45 LTTGGVTTQTGTEE570 pKa = 4.01 FADD573 pKa = 4.09 LLVGDD578 pKa = 5.24 DD579 pKa = 4.02 FANRR583 pKa = 11.84 LQGLNGNDD591 pKa = 3.4 TLNGNGGDD599 pKa = 4.05 DD600 pKa = 4.18 FLDD603 pKa = 4.32 GGAGTDD609 pKa = 3.41 RR610 pKa = 11.84 VSGGAGNDD618 pKa = 3.81 DD619 pKa = 4.36 LDD621 pKa = 3.85 VGAGNGSFQYY631 pKa = 11.39 ADD633 pKa = 3.25 GGAGNDD639 pKa = 3.49 TYY641 pKa = 11.14 HH642 pKa = 6.09 YY643 pKa = 10.63 AKK645 pKa = 10.67 DD646 pKa = 3.26 AGRR649 pKa = 11.84 LYY651 pKa = 11.08 LSGEE655 pKa = 4.32 TASSGSADD663 pKa = 3.76 RR664 pKa = 11.84 IVFSDD669 pKa = 3.86 LTLDD673 pKa = 4.72 DD674 pKa = 4.29 FTFDD678 pKa = 3.43 TLDD681 pKa = 3.94 YY682 pKa = 11.33 GPLPYY687 pKa = 10.86 GGDD690 pKa = 3.43 QVALRR695 pKa = 11.84 MLWNDD700 pKa = 3.67 GEE702 pKa = 4.61 SSGEE706 pKa = 3.72 LRR708 pKa = 11.84 VAA710 pKa = 4.02
Molecular weight: 74.73 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.694
IPC2_protein 3.808
IPC_protein 3.859
Toseland 3.63
ProMoST 4.024
Dawson 3.859
Bjellqvist 4.012
Wikipedia 3.795
Rodwell 3.681
Grimsley 3.541
Solomon 3.859
Lehninger 3.808
Nozaki 3.961
DTASelect 4.24
Thurlkill 3.681
EMBOSS 3.808
Sillero 3.986
Patrickios 1.125
IPC_peptide 3.846
IPC2_peptide 3.961
IPC2.peptide.svr19 3.856
Protein with the highest isoelectric point:
>tr|A0A1N7M9Q7|A0A1N7M9Q7_9RHOB L-glutamine ABC transporter membrane protein /L-glutamate ABC transporter membrane protein /L-aspartate ABC transporter membrane protein /L-asparagine ABC transporter membrane protein OS=Roseivivax lentus OX=633194 GN=SAMN05421759_104115 PE=3 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.01 RR4 pKa = 11.84 TFQPSNLVRR13 pKa = 11.84 KK14 pKa = 9.18 RR15 pKa = 11.84 RR16 pKa = 11.84 HH17 pKa = 4.42 GFRR20 pKa = 11.84 ARR22 pKa = 11.84 MATKK26 pKa = 10.37 AGRR29 pKa = 11.84 KK30 pKa = 8.54 ILNARR35 pKa = 11.84 RR36 pKa = 11.84 ARR38 pKa = 11.84 GRR40 pKa = 11.84 KK41 pKa = 9.07 SLSAA45 pKa = 3.93
Molecular weight: 5.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.676
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.398
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.177
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4153
0
4153
1304865
24
2538
314.2
34.01
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.141 ± 0.063
0.869 ± 0.013
6.212 ± 0.034
6.045 ± 0.038
3.71 ± 0.025
8.802 ± 0.042
2.017 ± 0.017
5.005 ± 0.03
2.755 ± 0.027
10.046 ± 0.041
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.675 ± 0.022
2.34 ± 0.02
5.31 ± 0.028
2.93 ± 0.019
7.126 ± 0.043
4.934 ± 0.026
5.433 ± 0.029
7.079 ± 0.029
1.417 ± 0.017
2.155 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here