Kordiimonas lacus

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Kordiimonadales; Kordiimonadaceae; Kordiimonas

Average proteome isoelectric point is 6.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3653 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1G6XKV3|A0A1G6XKV3_9PROT NAD(+) diphosphatase OS=Kordiimonas lacus OX=637679 GN=SAMN04488071_1277 PE=4 SV=1
MM1 pKa = 7.64GNTGTDD7 pKa = 2.9KK8 pKa = 10.95AAPQTADD15 pKa = 3.64DD16 pKa = 4.29TGDD19 pKa = 3.55GYY21 pKa = 9.29TIPFGDD27 pKa = 3.56FAGTYY32 pKa = 10.35VPFNDD37 pKa = 4.26PLFPFQANLTGFMYY51 pKa = 10.6DD52 pKa = 3.12DD53 pKa = 3.52TGAYY57 pKa = 8.52TEE59 pKa = 4.45SAHH62 pKa = 7.61INILPVWDD70 pKa = 4.84EE71 pKa = 3.96YY72 pKa = 11.63DD73 pKa = 3.35GTGILIASVEE83 pKa = 4.53EE84 pKa = 3.74IDD86 pKa = 5.14FRR88 pKa = 11.84HH89 pKa = 6.69PDD91 pKa = 3.33LEE93 pKa = 4.46GAQVDD98 pKa = 4.1NAPVSYY104 pKa = 11.35EE105 pKa = 3.85EE106 pKa = 4.04MFQNIQHH113 pKa = 7.37DD114 pKa = 4.61YY115 pKa = 9.04THH117 pKa = 6.39GTSTAGIIGARR128 pKa = 11.84AGNGIGLAGVAHH140 pKa = 7.13GSSILAGYY148 pKa = 9.56SIYY151 pKa = 11.18NGVTAEE157 pKa = 4.37LNADD161 pKa = 3.18VDD163 pKa = 3.76IVTRR167 pKa = 11.84SVSGGFLLSSDD178 pKa = 4.19LNGGYY183 pKa = 10.04SGATDD188 pKa = 4.12FLAEE192 pKa = 4.22GRR194 pKa = 11.84DD195 pKa = 3.6GLGIVWVNGSGNWRR209 pKa = 11.84YY210 pKa = 10.34NDD212 pKa = 3.37STTDD216 pKa = 3.26ISWGLNEE223 pKa = 5.52LYY225 pKa = 10.65VLSVGAASPNGFVTVFSVAGSALHH249 pKa = 5.83MVSPLAQAFLGAKK262 pKa = 9.85SDD264 pKa = 3.82TTLSLDD270 pKa = 3.94RR271 pKa = 11.84PGNWGEE277 pKa = 3.87IKK279 pKa = 10.51ILGAEE284 pKa = 3.78ITEE287 pKa = 4.28FEE289 pKa = 4.34ITGQAPQEE297 pKa = 4.0PGFYY301 pKa = 10.2LQVVEE306 pKa = 5.06NSLAHH311 pKa = 6.52AALEE315 pKa = 4.3AGFEE319 pKa = 4.31DD320 pKa = 4.49GDD322 pKa = 4.2YY323 pKa = 10.77IFYY326 pKa = 10.9GGTSSSGPLVSGATALVLEE345 pKa = 4.62AASNNVFGDD354 pKa = 3.91TEE356 pKa = 4.62LGWRR360 pKa = 11.84DD361 pKa = 3.44VQEE364 pKa = 4.08IFALSSAHH372 pKa = 6.04TGSGFGITEE381 pKa = 3.91RR382 pKa = 11.84DD383 pKa = 3.79LYY385 pKa = 11.28DD386 pKa = 4.15HH387 pKa = 7.14EE388 pKa = 4.86LNSWIINGASILNGGGLHH406 pKa = 6.63WSADD410 pKa = 3.7YY411 pKa = 11.12GFGMLDD417 pKa = 2.49VHH419 pKa = 6.46AAVRR423 pKa = 11.84LAEE426 pKa = 4.12TWTMTRR432 pKa = 11.84HH433 pKa = 5.58SGNLVEE439 pKa = 4.68QSHH442 pKa = 6.01SFATASEE449 pKa = 4.49TITYY453 pKa = 9.93GADD456 pKa = 3.02LTFKK460 pKa = 10.51FVAGDD465 pKa = 3.61ADD467 pKa = 4.87AIDD470 pKa = 3.56IDD472 pKa = 4.47GVEE475 pKa = 3.96LQFDD479 pKa = 4.06IVHH482 pKa = 6.68DD483 pKa = 4.22AWPEE487 pKa = 3.74LEE489 pKa = 5.0MILISPSGTEE499 pKa = 3.95IILFDD504 pKa = 4.15TPEE507 pKa = 4.0LTASAPHH514 pKa = 6.51EE515 pKa = 4.33SANTGQLEE523 pKa = 4.1WDD525 pKa = 3.66LLARR529 pKa = 11.84GFWGEE534 pKa = 3.86EE535 pKa = 4.07SAGEE539 pKa = 3.93WTLVIRR545 pKa = 11.84DD546 pKa = 3.83TVDD549 pKa = 2.78NGNSGTVNSLSLMFLGDD566 pKa = 3.55AATDD570 pKa = 3.45DD571 pKa = 4.77DD572 pKa = 5.0IYY574 pKa = 11.53YY575 pKa = 8.22FTDD578 pKa = 3.54DD579 pKa = 3.56WALMNAANGGVPVLDD594 pKa = 4.84DD595 pKa = 4.59ALGQNSINAAALMEE609 pKa = 5.31AILLKK614 pKa = 10.36LAPGSTSKK622 pKa = 10.55IGGEE626 pKa = 4.21TAFKK630 pKa = 10.12TSADD634 pKa = 3.38AVFTLGIGTDD644 pKa = 3.4QDD646 pKa = 3.73DD647 pKa = 4.57TLVGAAFDD655 pKa = 3.61EE656 pKa = 4.68TLYY659 pKa = 11.39GMQGDD664 pKa = 3.86DD665 pKa = 4.85RR666 pKa = 11.84MWAGAGDD673 pKa = 3.88VGADD677 pKa = 3.41SYY679 pKa = 11.89YY680 pKa = 11.11GGDD683 pKa = 3.53GADD686 pKa = 3.52IIGVGAGNDD695 pKa = 3.77LAVGEE700 pKa = 4.94AGNDD704 pKa = 3.91AIFGGAGDD712 pKa = 4.01DD713 pKa = 3.84TLVGGFWEE721 pKa = 5.32GGSASAVGGGHH732 pKa = 6.06NEE734 pKa = 3.63LWAGSGDD741 pKa = 4.39DD742 pKa = 4.6VIHH745 pKa = 6.55GANGNDD751 pKa = 3.33ALGGGTEE758 pKa = 4.09SDD760 pKa = 3.52TIYY763 pKa = 10.64GYY765 pKa = 10.79GGHH768 pKa = 7.76DD769 pKa = 2.99ILYY772 pKa = 8.99AGKK775 pKa = 10.25GAVSDD780 pKa = 4.2TLDD783 pKa = 3.64GGAGQDD789 pKa = 3.45TLFGGGGDD797 pKa = 4.26DD798 pKa = 3.67MLSGGGGNDD807 pKa = 4.71LIFNGAGSDD816 pKa = 3.53TATGGDD822 pKa = 4.1GDD824 pKa = 3.95DD825 pKa = 4.15TLWGGGGDD833 pKa = 4.14DD834 pKa = 5.25LLTGGTGADD843 pKa = 2.65IFAFTSTNGSDD854 pKa = 2.98TVTDD858 pKa = 4.08FDD860 pKa = 3.91AAEE863 pKa = 5.51DD864 pKa = 3.59ILLLDD869 pKa = 4.17GSGFADD875 pKa = 4.74LAALKK880 pKa = 10.44AATTEE885 pKa = 4.25DD886 pKa = 3.82GAGVTISFGDD896 pKa = 3.55TVIILSGISLDD907 pKa = 4.71DD908 pKa = 3.38LTAANVMLL916 pKa = 4.98

Molecular weight: 94.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1G7EK86|A0A1G7EK86_9PROT DNA gyrase subunit B OS=Kordiimonas lacus OX=637679 GN=gyrB PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLVRR12 pKa = 11.84KK13 pKa = 9.31RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.5GFRR19 pKa = 11.84ARR21 pKa = 11.84KK22 pKa = 7.71ATVGGRR28 pKa = 11.84AVLRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.77GRR39 pKa = 11.84KK40 pKa = 8.91RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3653

0

3653

1205558

36

2644

330.0

36.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.912 ± 0.051

0.831 ± 0.014

6.447 ± 0.045

6.217 ± 0.042

4.073 ± 0.029

8.416 ± 0.051

2.104 ± 0.021

5.299 ± 0.03

4.403 ± 0.036

9.751 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.85 ± 0.02

3.015 ± 0.027

4.537 ± 0.028

3.201 ± 0.022

5.809 ± 0.042

5.498 ± 0.038

5.5 ± 0.032

7.2 ± 0.031

1.303 ± 0.016

2.632 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski