Protomyces lactucaedebilis

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; Taphrinomycotina; Taphrinomycetes; Taphrinales; Protomycetaceae; Protomyces

Average proteome isoelectric point is 6.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6702 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Y2FSG1|A0A1Y2FSG1_PROLT Uncharacterized protein OS=Protomyces lactucaedebilis OX=56484 GN=BCR37DRAFT_390610 PE=4 SV=1
MM1 pKa = 7.57SEE3 pKa = 3.71GGFYY7 pKa = 10.96DD8 pKa = 3.47EE9 pKa = 6.18VEE11 pKa = 4.13IEE13 pKa = 5.1DD14 pKa = 3.54MSFDD18 pKa = 3.76KK19 pKa = 10.92EE20 pKa = 3.84KK21 pKa = 11.11NLYY24 pKa = 9.99HH25 pKa = 6.5FPCPCGDD32 pKa = 3.17RR33 pKa = 11.84FEE35 pKa = 4.43ITVEE39 pKa = 3.88QLKK42 pKa = 10.82EE43 pKa = 3.75GDD45 pKa = 4.46DD46 pKa = 3.55IARR49 pKa = 11.84CPSCSLIILVIYY61 pKa = 10.25DD62 pKa = 3.4QDD64 pKa = 4.59DD65 pKa = 3.87YY66 pKa = 12.05LSDD69 pKa = 3.79EE70 pKa = 4.42EE71 pKa = 4.69EE72 pKa = 4.25EE73 pKa = 4.64GVLIPTNEE81 pKa = 3.98KK82 pKa = 10.55VEE84 pKa = 4.09ALTAVAAA91 pKa = 4.37

Molecular weight: 10.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Y2FSF1|A0A1Y2FSF1_PROLT Uncharacterized protein (Fragment) OS=Protomyces lactucaedebilis OX=56484 GN=BCR37DRAFT_376251 PE=4 SV=1
RR1 pKa = 5.88YY2 pKa = 7.48TCPEE6 pKa = 3.92CQKK9 pKa = 9.38TFSRR13 pKa = 11.84PSSLRR18 pKa = 11.84IHH20 pKa = 6.03KK21 pKa = 9.96HH22 pKa = 3.53SHH24 pKa = 4.58TGEE27 pKa = 3.84RR28 pKa = 11.84PYY30 pKa = 10.99VCNVKK35 pKa = 10.02GCEE38 pKa = 3.65RR39 pKa = 11.84AFSVRR44 pKa = 11.84SNLRR48 pKa = 11.84RR49 pKa = 11.84HH50 pKa = 5.45MKK52 pKa = 9.56II53 pKa = 3.33

Molecular weight: 6.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6702

0

6702

2729480

49

5352

407.3

45.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.682 ± 0.03

1.48 ± 0.014

5.52 ± 0.022

5.902 ± 0.027

3.718 ± 0.018

6.302 ± 0.028

2.491 ± 0.015

4.705 ± 0.021

5.093 ± 0.025

9.791 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.299 ± 0.011

3.178 ± 0.014

5.17 ± 0.029

4.774 ± 0.026

5.919 ± 0.023

7.829 ± 0.029

5.911 ± 0.02

6.317 ± 0.021

1.239 ± 0.011

2.679 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski