Zhihengliuella halotolerans

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Micrococcaceae; Zhihengliuella

Average proteome isoelectric point is 5.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3241 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Q8AAY5|A0A4Q8AAY5_9MICC Amino acid ABC transporter ATP-binding protein (PAAT family) OS=Zhihengliuella halotolerans OX=370736 GN=EV380_0213 PE=4 SV=1
MM1 pKa = 6.94TRR3 pKa = 11.84KK4 pKa = 8.3STRR7 pKa = 11.84AVGLVASLAVAAGALAGCGQSGDD30 pKa = 4.03PDD32 pKa = 3.73SLTFMFRR39 pKa = 11.84GSPDD43 pKa = 2.9EE44 pKa = 3.95EE45 pKa = 4.0AAYY48 pKa = 9.47TEE50 pKa = 4.71AIEE53 pKa = 4.57AFEE56 pKa = 4.58AEE58 pKa = 4.43SGIEE62 pKa = 3.59VDD64 pKa = 5.1MIMTTADD71 pKa = 3.14EE72 pKa = 4.55YY73 pKa = 10.5ATKK76 pKa = 10.54LRR78 pKa = 11.84AAIVGGQIPDD88 pKa = 3.16VFYY91 pKa = 10.74FDD93 pKa = 4.31PGSVEE98 pKa = 4.35SYY100 pKa = 11.17ANAGVIQEE108 pKa = 3.84ITHH111 pKa = 6.21YY112 pKa = 10.46IEE114 pKa = 5.23ASDD117 pKa = 5.41VVDD120 pKa = 5.59LDD122 pKa = 4.09NMWDD126 pKa = 3.71YY127 pKa = 11.81GVDD130 pKa = 3.15SYY132 pKa = 11.73RR133 pKa = 11.84YY134 pKa = 9.99DD135 pKa = 3.61GEE137 pKa = 4.14QLGRR141 pKa = 11.84GPLYY145 pKa = 10.56ALPKK149 pKa = 10.41DD150 pKa = 3.59VGPFSFGYY158 pKa = 9.07NATMLEE164 pKa = 4.13EE165 pKa = 5.09AGIEE169 pKa = 4.2LPDD172 pKa = 4.38PDD174 pKa = 5.53EE175 pKa = 4.96PYY177 pKa = 9.73TWEE180 pKa = 3.7EE181 pKa = 3.47WLEE184 pKa = 3.78ILEE187 pKa = 4.81EE188 pKa = 4.25VTQDD192 pKa = 2.9TDD194 pKa = 3.8GDD196 pKa = 4.09GKK198 pKa = 9.17TDD200 pKa = 3.09QWGTGLNVTWNLQAFAWSNGGEE222 pKa = 4.27WLNEE226 pKa = 3.98DD227 pKa = 3.56ATQVTVDD234 pKa = 3.42TPEE237 pKa = 3.76FAEE240 pKa = 4.09ALQYY244 pKa = 10.94FSDD247 pKa = 4.35LDD249 pKa = 3.61TEE251 pKa = 4.58YY252 pKa = 11.41GVTPSIAEE260 pKa = 3.99AQTLDD265 pKa = 3.23TYY267 pKa = 9.55QRR269 pKa = 11.84WMQGQIAFFPIAPWDD284 pKa = 3.43IPVYY288 pKa = 10.38QDD290 pKa = 6.21LDD292 pKa = 4.22FEE294 pKa = 4.91WDD296 pKa = 3.51LMPYY300 pKa = 9.62PVGRR304 pKa = 11.84TGEE307 pKa = 4.0PASWIGTLGIGVSAATANPQAAADD331 pKa = 4.16LVAHH335 pKa = 6.74LSADD339 pKa = 3.61PDD341 pKa = 4.08TQRR344 pKa = 11.84SLAEE348 pKa = 4.3AGVQIPNLIDD358 pKa = 3.34MAHH361 pKa = 6.72EE362 pKa = 4.36FAADD366 pKa = 3.73DD367 pKa = 3.65STPPVNKK374 pKa = 9.92QEE376 pKa = 4.02FLDD379 pKa = 4.26VIEE382 pKa = 5.33DD383 pKa = 3.62YY384 pKa = 11.4GRR386 pKa = 11.84ALPPTYY392 pKa = 9.75TYY394 pKa = 11.1NPQWYY399 pKa = 9.55DD400 pKa = 3.38EE401 pKa = 5.18LYY403 pKa = 10.87TNIQPVLEE411 pKa = 4.29GRR413 pKa = 11.84KK414 pKa = 7.13TAADD418 pKa = 3.97YY419 pKa = 10.73LAEE422 pKa = 4.34AQPRR426 pKa = 11.84MQAFLDD432 pKa = 3.7MANDD436 pKa = 3.45QSEE439 pKa = 4.37MSRR442 pKa = 11.84GTKK445 pKa = 9.6

Molecular weight: 48.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Q8AF36|A0A4Q8AF36_9MICC DNA-binding protein HU-beta OS=Zhihengliuella halotolerans OX=370736 GN=EV380_2057 PE=3 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.56LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3241

0

3241

1082384

31

2601

334.0

35.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.549 ± 0.059

0.595 ± 0.01

6.098 ± 0.042

6.209 ± 0.043

3.203 ± 0.03

9.138 ± 0.04

2.102 ± 0.018

4.199 ± 0.036

2.234 ± 0.033

9.992 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.957 ± 0.018

2.231 ± 0.027

5.259 ± 0.032

2.794 ± 0.023

6.893 ± 0.051

5.606 ± 0.033

5.846 ± 0.034

8.582 ± 0.041

1.469 ± 0.018

2.047 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski