Salmonella phage 65FD

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Uetakevirus; unclassified Uetakevirus

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 48 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7D7JPE2|A0A7D7JPE2_9CAUD Putative DNA replication protein OS=Salmonella phage 65FD OX=2758312 GN=PIGZ_00027 PE=4 SV=1
MM1 pKa = 7.94DD2 pKa = 5.41LVNLKK7 pKa = 9.27TGTDD11 pKa = 3.44TYY13 pKa = 11.23QDD15 pKa = 3.97EE16 pKa = 4.44EE17 pKa = 5.16GKK19 pKa = 8.1TQTRR23 pKa = 11.84DD24 pKa = 3.24DD25 pKa = 4.54YY26 pKa = 11.56PWGLCIEE33 pKa = 4.82LNNEE37 pKa = 3.73TLAKK41 pKa = 10.32LKK43 pKa = 10.31AAPQSAGTEE52 pKa = 3.99VMITAKK58 pKa = 9.75ATIRR62 pKa = 11.84STSTRR67 pKa = 11.84EE68 pKa = 4.04TEE70 pKa = 5.72DD71 pKa = 3.45GMQHH75 pKa = 5.98NASLQITDD83 pKa = 3.71MALSPVSGEE92 pKa = 4.11QPKK95 pKa = 10.49SAAQTLYY102 pKa = 10.96GGEE105 pKa = 4.23DD106 pKa = 3.52DD107 pKa = 4.84

Molecular weight: 11.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7D7EXE8|A0A7D7EXE8_9CAUD Major capsid protein OS=Salmonella phage 65FD OX=2758312 GN=PIGZ_00010 PE=4 SV=1
MM1 pKa = 7.72VRR3 pKa = 11.84MVFIPDD9 pKa = 2.82RR10 pKa = 11.84RR11 pKa = 11.84FRR13 pKa = 11.84LAVSAGGNFQRR24 pKa = 11.84RR25 pKa = 11.84LDD27 pKa = 3.94PMSKK31 pKa = 8.89LTRR34 pKa = 11.84LEE36 pKa = 4.39KK37 pKa = 10.42YY38 pKa = 9.09HH39 pKa = 6.74LNYY42 pKa = 10.05VSQRR46 pKa = 11.84QASKK50 pKa = 10.96VVAVTPAAMEE60 pKa = 4.06VEE62 pKa = 4.0KK63 pKa = 10.77RR64 pKa = 11.84AVEE67 pKa = 4.11RR68 pKa = 11.84EE69 pKa = 4.02SKK71 pKa = 9.44GQYY74 pKa = 9.79RR75 pKa = 11.84IAARR79 pKa = 11.84LWLLCMDD86 pKa = 4.75AAVGEE91 pKa = 4.37VEE93 pKa = 4.24RR94 pKa = 11.84ARR96 pKa = 11.84IAIRR100 pKa = 11.84RR101 pKa = 11.84DD102 pKa = 3.16QCISKK107 pKa = 10.94GNGLRR112 pKa = 11.84RR113 pKa = 11.84GEE115 pKa = 3.99YY116 pKa = 10.35AGIGCRR122 pKa = 11.84GVVYY126 pKa = 10.43DD127 pKa = 4.23

Molecular weight: 14.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

48

0

48

12257

55

836

255.4

28.14

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.247 ± 0.61

0.987 ± 0.116

6.168 ± 0.252

5.809 ± 0.346

3.337 ± 0.212

7.8 ± 0.304

1.673 ± 0.162

5.474 ± 0.38

4.773 ± 0.298

7.718 ± 0.297

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.643 ± 0.268

4.593 ± 0.285

4.242 ± 0.175

4.748 ± 0.482

5.833 ± 0.308

6.69 ± 0.324

5.719 ± 0.373

6.731 ± 0.281

1.632 ± 0.146

3.182 ± 0.275
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski