Amino acid dipepetide frequency for Salmonella phage 65FD

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
13.708AlaAla: 13.708 ± 1.879
0.49AlaCys: 0.49 ± 0.199
5.711AlaAsp: 5.711 ± 0.849
6.527AlaGlu: 6.527 ± 1.334
3.835AlaPhe: 3.835 ± 0.424
7.833AlaGly: 7.833 ± 1.012
0.979AlaHis: 0.979 ± 0.255
3.835AlaIle: 3.835 ± 0.621
3.753AlaLys: 3.753 ± 0.725
8.159AlaLeu: 8.159 ± 0.931
3.427AlaMet: 3.427 ± 0.62
3.835AlaAsn: 3.835 ± 0.548
3.835AlaPro: 3.835 ± 0.643
6.609AlaGln: 6.609 ± 1.193
6.201AlaArg: 6.201 ± 0.949
7.017AlaSer: 7.017 ± 0.613
4.08AlaThr: 4.08 ± 0.558
7.914AlaVal: 7.914 ± 0.899
1.224AlaTrp: 1.224 ± 0.307
3.508AlaTyr: 3.508 ± 0.664
0.0AlaXaa: 0.0 ± 0.0
Cys
0.816CysAla: 0.816 ± 0.245
0.163CysCys: 0.163 ± 0.11
0.571CysAsp: 0.571 ± 0.197
0.571CysGlu: 0.571 ± 0.237
0.571CysPhe: 0.571 ± 0.208
1.061CysGly: 1.061 ± 0.367
0.245CysHis: 0.245 ± 0.112
0.816CysIle: 0.816 ± 0.268
0.326CysLys: 0.326 ± 0.197
0.734CysLeu: 0.734 ± 0.27
0.163CysMet: 0.163 ± 0.102
0.408CysAsn: 0.408 ± 0.196
0.734CysPro: 0.734 ± 0.249
0.163CysGln: 0.163 ± 0.11
0.979CysArg: 0.979 ± 0.295
0.408CysSer: 0.408 ± 0.203
0.245CysThr: 0.245 ± 0.173
0.571CysVal: 0.571 ± 0.257
0.245CysTrp: 0.245 ± 0.164
0.082CysTyr: 0.082 ± 0.069
0.0CysXaa: 0.0 ± 0.0
Asp
6.201AspAla: 6.201 ± 0.862
0.898AspCys: 0.898 ± 0.279
3.998AspAsp: 3.998 ± 0.557
3.998AspGlu: 3.998 ± 0.851
1.958AspPhe: 1.958 ± 0.354
5.304AspGly: 5.304 ± 0.742
0.898AspHis: 0.898 ± 0.344
3.427AspIle: 3.427 ± 0.533
3.672AspLys: 3.672 ± 0.671
5.059AspLeu: 5.059 ± 0.531
2.529AspMet: 2.529 ± 0.554
2.203AspAsn: 2.203 ± 0.391
2.366AspPro: 2.366 ± 0.607
1.958AspGln: 1.958 ± 0.393
2.203AspArg: 2.203 ± 0.389
3.672AspSer: 3.672 ± 0.413
2.529AspThr: 2.529 ± 0.556
4.977AspVal: 4.977 ± 0.825
1.469AspTrp: 1.469 ± 0.378
2.366AspTyr: 2.366 ± 0.507
0.0AspXaa: 0.0 ± 0.0
Glu
4.732GluAla: 4.732 ± 0.744
0.326GluCys: 0.326 ± 0.168
3.182GluAsp: 3.182 ± 0.506
3.508GluGlu: 3.508 ± 0.586
2.611GluPhe: 2.611 ± 0.51
3.182GluGly: 3.182 ± 0.604
0.979GluHis: 0.979 ± 0.254
2.937GluIle: 2.937 ± 0.49
3.182GluLys: 3.182 ± 0.587
5.63GluLeu: 5.63 ± 0.835
0.979GluMet: 0.979 ± 0.314
1.958GluAsn: 1.958 ± 0.351
1.958GluPro: 1.958 ± 0.491
4.732GluGln: 4.732 ± 0.713
4.08GluArg: 4.08 ± 0.806
3.59GluSer: 3.59 ± 0.522
3.182GluThr: 3.182 ± 0.395
4.324GluVal: 4.324 ± 0.748
1.061GluTrp: 1.061 ± 0.296
1.958GluTyr: 1.958 ± 0.255
0.0GluXaa: 0.0 ± 0.0
Phe
2.611PheAla: 2.611 ± 0.506
0.326PheCys: 0.326 ± 0.181
2.121PheAsp: 2.121 ± 0.495
2.203PheGlu: 2.203 ± 0.36
1.305PhePhe: 1.305 ± 0.299
2.693PheGly: 2.693 ± 0.483
0.571PheHis: 0.571 ± 0.225
2.693PheIle: 2.693 ± 0.497
1.958PheLys: 1.958 ± 0.405
1.713PheLeu: 1.713 ± 0.392
0.408PheMet: 0.408 ± 0.153
1.632PheAsn: 1.632 ± 0.293
1.469PhePro: 1.469 ± 0.325
0.979PheGln: 0.979 ± 0.415
2.04PheArg: 2.04 ± 0.389
2.856PheSer: 2.856 ± 0.495
2.04PheThr: 2.04 ± 0.43
1.713PheVal: 1.713 ± 0.452
0.653PheTrp: 0.653 ± 0.212
1.387PheTyr: 1.387 ± 0.414
0.0PheXaa: 0.0 ± 0.0
Gly
6.038GlyAla: 6.038 ± 0.776
0.898GlyCys: 0.898 ± 0.301
4.896GlyAsp: 4.896 ± 0.673
4.488GlyGlu: 4.488 ± 0.43
3.101GlyPhe: 3.101 ± 0.65
5.711GlyGly: 5.711 ± 0.75
1.632GlyHis: 1.632 ± 0.418
5.222GlyIle: 5.222 ± 0.788
5.14GlyLys: 5.14 ± 0.742
5.793GlyLeu: 5.793 ± 0.857
2.611GlyMet: 2.611 ± 0.445
3.672GlyAsn: 3.672 ± 0.551
2.04GlyPro: 2.04 ± 0.376
3.019GlyGln: 3.019 ± 0.656
4.08GlyArg: 4.08 ± 0.673
5.63GlySer: 5.63 ± 0.902
5.14GlyThr: 5.14 ± 0.814
5.711GlyVal: 5.711 ± 0.858
1.142GlyTrp: 1.142 ± 0.305
2.04GlyTyr: 2.04 ± 0.363
0.0GlyXaa: 0.0 ± 0.0
His
1.55HisAla: 1.55 ± 0.444
0.408HisCys: 0.408 ± 0.221
0.979HisAsp: 0.979 ± 0.231
1.061HisGlu: 1.061 ± 0.31
0.408HisPhe: 0.408 ± 0.225
1.55HisGly: 1.55 ± 0.369
0.408HisHis: 0.408 ± 0.165
0.979HisIle: 0.979 ± 0.263
0.49HisLys: 0.49 ± 0.2
1.877HisLeu: 1.877 ± 0.459
0.571HisMet: 0.571 ± 0.191
0.734HisAsn: 0.734 ± 0.253
0.734HisPro: 0.734 ± 0.233
0.408HisGln: 0.408 ± 0.205
0.734HisArg: 0.734 ± 0.229
1.061HisSer: 1.061 ± 0.371
0.734HisThr: 0.734 ± 0.231
0.979HisVal: 0.979 ± 0.264
0.571HisTrp: 0.571 ± 0.176
0.49HisTyr: 0.49 ± 0.182
0.0HisXaa: 0.0 ± 0.0
Ile
5.385IleAla: 5.385 ± 0.635
0.571IleCys: 0.571 ± 0.215
4.406IleAsp: 4.406 ± 0.597
2.937IleGlu: 2.937 ± 0.42
1.877IlePhe: 1.877 ± 0.471
4.651IleGly: 4.651 ± 0.627
0.734IleHis: 0.734 ± 0.255
2.856IleIle: 2.856 ± 0.879
2.693IleLys: 2.693 ± 0.599
3.59IleLeu: 3.59 ± 0.761
0.734IleMet: 0.734 ± 0.245
3.508IleAsn: 3.508 ± 0.481
2.448IlePro: 2.448 ± 0.346
2.285IleGln: 2.285 ± 0.626
3.101IleArg: 3.101 ± 0.466
3.916IleSer: 3.916 ± 0.732
3.835IleThr: 3.835 ± 0.583
3.019IleVal: 3.019 ± 0.686
0.898IleTrp: 0.898 ± 0.212
1.305IleTyr: 1.305 ± 0.312
0.0IleXaa: 0.0 ± 0.0
Lys
4.814LysAla: 4.814 ± 0.649
0.734LysCys: 0.734 ± 0.292
1.958LysAsp: 1.958 ± 0.424
2.611LysGlu: 2.611 ± 0.531
1.224LysPhe: 1.224 ± 0.29
2.937LysGly: 2.937 ± 0.711
0.734LysHis: 0.734 ± 0.226
1.55LysIle: 1.55 ± 0.266
2.285LysLys: 2.285 ± 0.406
3.835LysLeu: 3.835 ± 0.588
1.713LysMet: 1.713 ± 0.462
2.285LysAsn: 2.285 ± 0.595
3.345LysPro: 3.345 ± 0.757
1.877LysGln: 1.877 ± 0.438
3.753LysArg: 3.753 ± 0.696
3.753LysSer: 3.753 ± 0.668
2.611LysThr: 2.611 ± 0.417
3.264LysVal: 3.264 ± 0.63
0.816LysTrp: 0.816 ± 0.334
1.632LysTyr: 1.632 ± 0.523
0.0LysXaa: 0.0 ± 0.0
Leu
8.73LeuAla: 8.73 ± 1.184
0.816LeuCys: 0.816 ± 0.267
4.651LeuAsp: 4.651 ± 0.601
4.324LeuGlu: 4.324 ± 0.564
2.856LeuPhe: 2.856 ± 0.562
4.651LeuGly: 4.651 ± 1.008
1.305LeuHis: 1.305 ± 0.342
4.406LeuIle: 4.406 ± 0.784
3.998LeuLys: 3.998 ± 0.543
5.711LeuLeu: 5.711 ± 0.796
1.55LeuMet: 1.55 ± 0.297
3.264LeuAsn: 3.264 ± 0.545
2.937LeuPro: 2.937 ± 0.439
3.101LeuGln: 3.101 ± 0.546
5.14LeuArg: 5.14 ± 0.614
6.364LeuSer: 6.364 ± 0.88
6.364LeuThr: 6.364 ± 0.786
4.161LeuVal: 4.161 ± 0.52
1.142LeuTrp: 1.142 ± 0.397
1.713LeuTyr: 1.713 ± 0.379
0.0LeuXaa: 0.0 ± 0.0
Met
3.835MetAla: 3.835 ± 0.533
0.326MetCys: 0.326 ± 0.154
1.55MetAsp: 1.55 ± 0.433
1.061MetGlu: 1.061 ± 0.326
0.408MetPhe: 0.408 ± 0.197
2.04MetGly: 2.04 ± 0.554
0.0MetHis: 0.0 ± 0.0
1.55MetIle: 1.55 ± 0.443
1.142MetLys: 1.142 ± 0.47
1.795MetLeu: 1.795 ± 0.447
1.061MetMet: 1.061 ± 0.314
1.632MetAsn: 1.632 ± 0.37
1.55MetPro: 1.55 ± 0.411
0.898MetGln: 0.898 ± 0.283
0.979MetArg: 0.979 ± 0.344
2.121MetSer: 2.121 ± 0.398
1.55MetThr: 1.55 ± 0.325
1.795MetVal: 1.795 ± 0.521
0.163MetTrp: 0.163 ± 0.112
0.979MetTyr: 0.979 ± 0.373
0.0MetXaa: 0.0 ± 0.0
Asn
4.08AsnAla: 4.08 ± 0.547
0.163AsnCys: 0.163 ± 0.109
2.529AsnAsp: 2.529 ± 0.473
3.019AsnGlu: 3.019 ± 0.631
0.816AsnPhe: 0.816 ± 0.214
4.732AsnGly: 4.732 ± 0.681
0.979AsnHis: 0.979 ± 0.286
2.693AsnIle: 2.693 ± 0.583
1.632AsnLys: 1.632 ± 0.412
3.835AsnLeu: 3.835 ± 0.735
0.734AsnMet: 0.734 ± 0.257
2.529AsnAsn: 2.529 ± 0.639
2.285AsnPro: 2.285 ± 0.518
2.529AsnGln: 2.529 ± 0.523
2.121AsnArg: 2.121 ± 0.424
3.182AsnSer: 3.182 ± 0.51
2.611AsnThr: 2.611 ± 0.451
2.366AsnVal: 2.366 ± 0.457
0.816AsnTrp: 0.816 ± 0.314
2.285AsnTyr: 2.285 ± 0.57
0.0AsnXaa: 0.0 ± 0.0
Pro
4.406ProAla: 4.406 ± 0.954
0.49ProCys: 0.49 ± 0.197
3.998ProAsp: 3.998 ± 0.647
3.508ProGlu: 3.508 ± 0.594
1.224ProPhe: 1.224 ± 0.326
3.672ProGly: 3.672 ± 0.513
0.571ProHis: 0.571 ± 0.238
2.203ProIle: 2.203 ± 0.355
0.979ProLys: 0.979 ± 0.272
2.774ProLeu: 2.774 ± 0.394
0.734ProMet: 0.734 ± 0.208
1.224ProAsn: 1.224 ± 0.321
1.877ProPro: 1.877 ± 0.441
2.366ProGln: 2.366 ± 0.433
1.713ProArg: 1.713 ± 0.438
2.529ProSer: 2.529 ± 0.485
2.448ProThr: 2.448 ± 0.467
3.753ProVal: 3.753 ± 0.58
0.653ProTrp: 0.653 ± 0.217
1.305ProTyr: 1.305 ± 0.346
0.0ProXaa: 0.0 ± 0.0
Gln
6.119GlnAla: 6.119 ± 1.486
0.571GlnCys: 0.571 ± 0.265
2.121GlnAsp: 2.121 ± 0.343
2.448GlnGlu: 2.448 ± 0.534
1.713GlnPhe: 1.713 ± 0.425
2.937GlnGly: 2.937 ± 0.541
0.979GlnHis: 0.979 ± 0.28
2.121GlnIle: 2.121 ± 0.641
1.877GlnLys: 1.877 ± 0.507
3.998GlnLeu: 3.998 ± 0.781
1.305GlnMet: 1.305 ± 0.372
1.55GlnAsn: 1.55 ± 0.318
1.958GlnPro: 1.958 ± 0.407
3.59GlnGln: 3.59 ± 0.983
3.916GlnArg: 3.916 ± 0.586
1.877GlnSer: 1.877 ± 0.344
3.101GlnThr: 3.101 ± 0.679
2.774GlnVal: 2.774 ± 0.61
0.734GlnTrp: 0.734 ± 0.314
1.795GlnTyr: 1.795 ± 0.354
0.0GlnXaa: 0.0 ± 0.0
Arg
5.793ArgAla: 5.793 ± 0.905
0.734ArgCys: 0.734 ± 0.27
4.324ArgAsp: 4.324 ± 0.58
3.345ArgGlu: 3.345 ± 0.581
1.795ArgPhe: 1.795 ± 0.314
3.182ArgGly: 3.182 ± 0.385
1.061ArgHis: 1.061 ± 0.274
3.916ArgIle: 3.916 ± 0.482
3.59ArgLys: 3.59 ± 0.391
4.732ArgLeu: 4.732 ± 0.64
1.387ArgMet: 1.387 ± 0.329
3.427ArgAsn: 3.427 ± 0.431
1.387ArgPro: 1.387 ± 0.464
3.101ArgGln: 3.101 ± 0.428
3.998ArgArg: 3.998 ± 0.723
3.264ArgSer: 3.264 ± 0.638
2.693ArgThr: 2.693 ± 0.483
3.427ArgVal: 3.427 ± 0.67
1.224ArgTrp: 1.224 ± 0.316
1.958ArgTyr: 1.958 ± 0.399
0.0ArgXaa: 0.0 ± 0.0
Ser
7.343SerAla: 7.343 ± 0.871
0.816SerCys: 0.816 ± 0.226
3.835SerAsp: 3.835 ± 0.546
4.243SerGlu: 4.243 ± 0.501
2.121SerPhe: 2.121 ± 0.476
6.691SerGly: 6.691 ± 0.79
1.387SerHis: 1.387 ± 0.322
3.345SerIle: 3.345 ± 0.546
3.427SerLys: 3.427 ± 0.528
4.324SerLeu: 4.324 ± 0.716
1.795SerMet: 1.795 ± 0.442
3.264SerAsn: 3.264 ± 0.577
3.182SerPro: 3.182 ± 0.462
2.856SerGln: 2.856 ± 0.615
3.672SerArg: 3.672 ± 0.482
3.508SerSer: 3.508 ± 0.622
3.59SerThr: 3.59 ± 0.596
5.059SerVal: 5.059 ± 0.684
0.571SerTrp: 0.571 ± 0.201
1.795SerTyr: 1.795 ± 0.622
0.0SerXaa: 0.0 ± 0.0
Thr
5.956ThrAla: 5.956 ± 0.978
0.326ThrCys: 0.326 ± 0.168
3.264ThrAsp: 3.264 ± 0.541
2.121ThrGlu: 2.121 ± 0.456
1.795ThrPhe: 1.795 ± 0.374
6.527ThrGly: 6.527 ± 0.897
1.061ThrHis: 1.061 ± 0.327
3.019ThrIle: 3.019 ± 0.595
2.04ThrLys: 2.04 ± 0.347
4.161ThrLeu: 4.161 ± 0.692
1.795ThrMet: 1.795 ± 0.392
2.529ThrAsn: 2.529 ± 0.558
3.345ThrPro: 3.345 ± 0.604
2.693ThrGln: 2.693 ± 0.59
2.693ThrArg: 2.693 ± 0.569
4.08ThrSer: 4.08 ± 0.664
2.937ThrThr: 2.937 ± 0.592
3.835ThrVal: 3.835 ± 0.736
1.224ThrTrp: 1.224 ± 0.282
1.795ThrTyr: 1.795 ± 0.319
0.0ThrXaa: 0.0 ± 0.0
Val
6.119ValAla: 6.119 ± 0.758
0.49ValCys: 0.49 ± 0.16
4.896ValAsp: 4.896 ± 0.546
3.59ValGlu: 3.59 ± 0.474
1.958ValPhe: 1.958 ± 0.387
3.916ValGly: 3.916 ± 0.556
0.979ValHis: 0.979 ± 0.279
4.814ValIle: 4.814 ± 0.709
3.427ValLys: 3.427 ± 0.387
5.385ValLeu: 5.385 ± 0.731
1.713ValMet: 1.713 ± 0.373
4.569ValAsn: 4.569 ± 0.578
2.448ValPro: 2.448 ± 0.373
2.448ValGln: 2.448 ± 0.527
4.08ValArg: 4.08 ± 0.584
4.651ValSer: 4.651 ± 0.664
4.161ValThr: 4.161 ± 0.622
4.814ValVal: 4.814 ± 0.804
0.898ValTrp: 0.898 ± 0.327
1.958ValTyr: 1.958 ± 0.369
0.0ValXaa: 0.0 ± 0.0
Trp
1.142TrpAla: 1.142 ± 0.356
0.163TrpCys: 0.163 ± 0.124
0.898TrpAsp: 0.898 ± 0.328
0.979TrpGlu: 0.979 ± 0.335
0.49TrpPhe: 0.49 ± 0.179
1.469TrpGly: 1.469 ± 0.327
0.326TrpHis: 0.326 ± 0.136
0.816TrpIle: 0.816 ± 0.185
0.816TrpLys: 0.816 ± 0.283
1.713TrpLeu: 1.713 ± 0.444
0.49TrpMet: 0.49 ± 0.189
0.571TrpAsn: 0.571 ± 0.201
0.734TrpPro: 0.734 ± 0.287
0.49TrpGln: 0.49 ± 0.222
0.816TrpArg: 0.816 ± 0.322
1.713TrpSer: 1.713 ± 0.45
1.061TrpThr: 1.061 ± 0.286
0.979TrpVal: 0.979 ± 0.307
0.245TrpTrp: 0.245 ± 0.139
0.408TrpTyr: 0.408 ± 0.148
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.101TyrAla: 3.101 ± 0.381
0.163TyrCys: 0.163 ± 0.121
1.795TyrAsp: 1.795 ± 0.323
1.55TyrGlu: 1.55 ± 0.333
1.305TyrPhe: 1.305 ± 0.467
3.264TyrGly: 3.264 ± 0.606
0.898TyrHis: 0.898 ± 0.344
1.55TyrIle: 1.55 ± 0.43
1.305TyrLys: 1.305 ± 0.356
2.366TyrLeu: 2.366 ± 0.411
0.653TyrMet: 0.653 ± 0.236
1.142TyrAsn: 1.142 ± 0.251
1.795TyrPro: 1.795 ± 0.514
1.387TyrGln: 1.387 ± 0.384
2.04TyrArg: 2.04 ± 0.351
1.713TyrSer: 1.713 ± 0.381
2.285TyrThr: 2.285 ± 0.504
1.877TyrVal: 1.877 ± 0.399
0.571TyrTrp: 0.571 ± 0.215
1.061TyrTyr: 1.061 ± 0.341
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 48 proteins (12257 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski