Amino acid dipepetide frequency for Bradyrhizobium sp. STM 3843

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
18.139AlaAla: 18.139 ± 0.124
1.213AlaCys: 1.213 ± 0.024
6.846AlaAsp: 6.846 ± 0.06
7.009AlaGlu: 7.009 ± 0.069
4.457AlaPhe: 4.457 ± 0.043
10.127AlaGly: 10.127 ± 0.075
2.355AlaHis: 2.355 ± 0.035
6.724AlaIle: 6.724 ± 0.057
4.47AlaLys: 4.47 ± 0.054
13.372AlaLeu: 13.372 ± 0.085
3.445AlaMet: 3.445 ± 0.042
3.172AlaAsn: 3.172 ± 0.041
5.94AlaPro: 5.94 ± 0.063
4.239AlaGln: 4.239 ± 0.052
8.723AlaArg: 8.723 ± 0.064
6.972AlaSer: 6.972 ± 0.061
6.426AlaThr: 6.426 ± 0.056
9.052AlaVal: 9.052 ± 0.064
1.495AlaTrp: 1.495 ± 0.023
2.581AlaTyr: 2.581 ± 0.031
0.0AlaXaa: 0.0 ± 0.0
Cys
1.068CysAla: 1.068 ± 0.022
0.132CysCys: 0.132 ± 0.008
0.538CysAsp: 0.538 ± 0.016
0.466CysGlu: 0.466 ± 0.015
0.344CysPhe: 0.344 ± 0.013
0.997CysGly: 0.997 ± 0.021
0.284CysHis: 0.284 ± 0.015
0.426CysIle: 0.426 ± 0.014
0.216CysLys: 0.216 ± 0.01
0.854CysLeu: 0.854 ± 0.019
0.187CysMet: 0.187 ± 0.009
0.244CysAsn: 0.244 ± 0.01
0.471CysPro: 0.471 ± 0.015
0.255CysGln: 0.255 ± 0.011
0.73CysArg: 0.73 ± 0.018
0.537CysSer: 0.537 ± 0.016
0.452CysThr: 0.452 ± 0.015
0.655CysVal: 0.655 ± 0.015
0.132CysTrp: 0.132 ± 0.007
0.232CysTyr: 0.232 ± 0.011
0.0CysXaa: 0.0 ± 0.0
Asp
6.724AspAla: 6.724 ± 0.059
0.492AspCys: 0.492 ± 0.015
3.077AspAsp: 3.077 ± 0.04
3.134AspGlu: 3.134 ± 0.041
2.102AspPhe: 2.102 ± 0.031
4.828AspGly: 4.828 ± 0.051
1.368AspHis: 1.368 ± 0.027
3.023AspIle: 3.023 ± 0.04
1.76AspLys: 1.76 ± 0.034
5.561AspLeu: 5.561 ± 0.059
1.173AspMet: 1.173 ± 0.022
1.32AspAsn: 1.32 ± 0.025
3.407AspPro: 3.407 ± 0.042
1.85AspGln: 1.85 ± 0.032
4.204AspArg: 4.204 ± 0.046
2.216AspSer: 2.216 ± 0.03
2.39AspThr: 2.39 ± 0.03
4.173AspVal: 4.173 ± 0.041
0.887AspTrp: 0.887 ± 0.022
1.465AspTyr: 1.465 ± 0.028
0.001AspXaa: 0.001 ± 0.001
Glu
6.877GluAla: 6.877 ± 0.065
0.365GluCys: 0.365 ± 0.013
2.272GluAsp: 2.272 ± 0.033
2.462GluGlu: 2.462 ± 0.034
1.734GluPhe: 1.734 ± 0.027
3.463GluGly: 3.463 ± 0.036
1.156GluHis: 1.156 ± 0.024
3.386GluIle: 3.386 ± 0.039
1.959GluLys: 1.959 ± 0.035
5.45GluLeu: 5.45 ± 0.055
1.325GluMet: 1.325 ± 0.025
1.306GluAsn: 1.306 ± 0.025
2.651GluPro: 2.651 ± 0.036
2.26GluGln: 2.26 ± 0.033
4.706GluArg: 4.706 ± 0.054
2.219GluSer: 2.219 ± 0.03
3.048GluThr: 3.048 ± 0.038
3.58GluVal: 3.58 ± 0.044
0.632GluTrp: 0.632 ± 0.016
0.89GluTyr: 0.89 ± 0.019
0.0GluXaa: 0.0 ± 0.0
Phe
4.904PheAla: 4.904 ± 0.052
0.422PheCys: 0.422 ± 0.016
2.498PheAsp: 2.498 ± 0.033
1.943PheGlu: 1.943 ± 0.027
1.403PhePhe: 1.403 ± 0.027
3.777PheGly: 3.777 ± 0.046
0.738PheHis: 0.738 ± 0.019
1.684PheIle: 1.684 ± 0.027
1.117PheLys: 1.117 ± 0.025
3.258PheLeu: 3.258 ± 0.039
0.768PheMet: 0.768 ± 0.019
1.139PheAsn: 1.139 ± 0.024
1.679PhePro: 1.679 ± 0.026
1.038PheGln: 1.038 ± 0.02
2.281PheArg: 2.281 ± 0.035
2.169PheSer: 2.169 ± 0.035
1.991PheThr: 1.991 ± 0.031
2.997PheVal: 2.997 ± 0.035
0.548PheTrp: 0.548 ± 0.014
0.88PheTyr: 0.88 ± 0.019
0.0PheXaa: 0.0 ± 0.0
Gly
9.161GlyAla: 9.161 ± 0.077
0.909GlyCys: 0.909 ± 0.02
4.11GlyAsp: 4.11 ± 0.039
4.214GlyGlu: 4.214 ± 0.051
3.579GlyPhe: 3.579 ± 0.038
7.083GlyGly: 7.083 ± 0.076
1.919GlyHis: 1.919 ± 0.03
4.462GlyIle: 4.462 ± 0.045
3.101GlyLys: 3.101 ± 0.039
8.633GlyLeu: 8.633 ± 0.063
2.013GlyMet: 2.013 ± 0.025
2.193GlyAsn: 2.193 ± 0.041
3.604GlyPro: 3.604 ± 0.042
2.846GlyGln: 2.846 ± 0.041
6.021GlyArg: 6.021 ± 0.052
4.71GlySer: 4.71 ± 0.05
4.553GlyThr: 4.553 ± 0.062
5.982GlyVal: 5.982 ± 0.055
1.354GlyTrp: 1.354 ± 0.029
2.298GlyTyr: 2.298 ± 0.032
0.0GlyXaa: 0.0 ± 0.0
His
2.439HisAla: 2.439 ± 0.031
0.254HisCys: 0.254 ± 0.01
1.31HisAsp: 1.31 ± 0.026
1.02HisGlu: 1.02 ± 0.021
0.873HisPhe: 0.873 ± 0.019
2.013HisGly: 2.013 ± 0.03
0.663HisHis: 0.663 ± 0.024
0.922HisIle: 0.922 ± 0.022
0.48HisLys: 0.48 ± 0.015
2.066HisLeu: 2.066 ± 0.035
0.488HisMet: 0.488 ± 0.014
0.499HisAsn: 0.499 ± 0.015
1.393HisPro: 1.393 ± 0.026
0.66HisGln: 0.66 ± 0.019
1.57HisArg: 1.57 ± 0.03
0.972HisSer: 0.972 ± 0.022
0.832HisThr: 0.832 ± 0.02
1.574HisVal: 1.574 ± 0.027
0.371HisTrp: 0.371 ± 0.012
0.549HisTyr: 0.549 ± 0.017
0.0HisXaa: 0.0 ± 0.0
Ile
7.809IleAla: 7.809 ± 0.064
0.561IleCys: 0.561 ± 0.016
3.542IleAsp: 3.542 ± 0.035
3.417IleGlu: 3.417 ± 0.037
1.705IlePhe: 1.705 ± 0.026
5.21IleGly: 5.21 ± 0.049
0.912IleHis: 0.912 ± 0.019
2.277IleIle: 2.277 ± 0.034
1.679IleLys: 1.679 ± 0.027
4.204IleLeu: 4.204 ± 0.046
1.036IleMet: 1.036 ± 0.023
1.47IleAsn: 1.47 ± 0.023
2.374IlePro: 2.374 ± 0.032
1.244IleGln: 1.244 ± 0.023
3.248IleArg: 3.248 ± 0.039
3.151IleSer: 3.151 ± 0.044
2.77IleThr: 2.77 ± 0.034
4.42IleVal: 4.42 ± 0.047
0.645IleTrp: 0.645 ± 0.018
1.202IleTyr: 1.202 ± 0.022
0.0IleXaa: 0.0 ± 0.0
Lys
4.224LysAla: 4.224 ± 0.051
0.181LysCys: 0.181 ± 0.009
1.759LysAsp: 1.759 ± 0.028
1.553LysGlu: 1.553 ± 0.03
0.995LysPhe: 0.995 ± 0.025
2.455LysGly: 2.455 ± 0.038
0.634LysHis: 0.634 ± 0.018
1.831LysIle: 1.831 ± 0.027
1.244LysLys: 1.244 ± 0.031
3.616LysLeu: 3.616 ± 0.04
0.82LysMet: 0.82 ± 0.019
0.818LysAsn: 0.818 ± 0.017
2.282LysPro: 2.282 ± 0.028
1.216LysGln: 1.216 ± 0.025
2.358LysArg: 2.358 ± 0.032
1.939LysSer: 1.939 ± 0.033
2.034LysThr: 2.034 ± 0.029
2.406LysVal: 2.406 ± 0.032
0.392LysTrp: 0.392 ± 0.014
0.67LysTyr: 0.67 ± 0.02
0.0LysXaa: 0.0 ± 0.0
Leu
13.756LeuAla: 13.756 ± 0.088
0.959LeuCys: 0.959 ± 0.022
5.697LeuAsp: 5.697 ± 0.048
4.508LeuGlu: 4.508 ± 0.042
3.62LeuPhe: 3.62 ± 0.043
8.189LeuGly: 8.189 ± 0.072
1.864LeuHis: 1.864 ± 0.035
5.287LeuIle: 5.287 ± 0.049
3.685LeuLys: 3.685 ± 0.045
9.819LeuLeu: 9.819 ± 0.071
2.276LeuMet: 2.276 ± 0.032
2.635LeuAsn: 2.635 ± 0.036
5.567LeuPro: 5.567 ± 0.048
3.055LeuGln: 3.055 ± 0.035
7.014LeuArg: 7.014 ± 0.061
6.586LeuSer: 6.586 ± 0.066
5.667LeuThr: 5.667 ± 0.052
7.502LeuVal: 7.502 ± 0.061
1.176LeuTrp: 1.176 ± 0.025
1.986LeuTyr: 1.986 ± 0.034
0.0LeuXaa: 0.0 ± 0.0
Met
2.837MetAla: 2.837 ± 0.039
0.156MetCys: 0.156 ± 0.008
0.989MetAsp: 0.989 ± 0.022
0.918MetGlu: 0.918 ± 0.023
0.764MetPhe: 0.764 ± 0.021
1.571MetGly: 1.571 ± 0.028
0.444MetHis: 0.444 ± 0.014
1.379MetIle: 1.379 ± 0.026
0.919MetLys: 0.919 ± 0.018
2.578MetLeu: 2.578 ± 0.033
0.685MetMet: 0.685 ± 0.018
0.703MetAsn: 0.703 ± 0.019
1.514MetPro: 1.514 ± 0.023
0.842MetGln: 0.842 ± 0.021
1.785MetArg: 1.785 ± 0.027
1.751MetSer: 1.751 ± 0.027
1.821MetThr: 1.821 ± 0.028
1.666MetVal: 1.666 ± 0.028
0.216MetTrp: 0.216 ± 0.009
0.344MetTyr: 0.344 ± 0.013
0.0MetXaa: 0.0 ± 0.0
Asn
3.311AsnAla: 3.311 ± 0.04
0.267AsnCys: 0.267 ± 0.01
1.441AsnAsp: 1.441 ± 0.024
1.292AsnGlu: 1.292 ± 0.026
1.02AsnPhe: 1.02 ± 0.023
2.438AsnGly: 2.438 ± 0.042
0.51AsnHis: 0.51 ± 0.016
1.354AsnIle: 1.354 ± 0.022
0.798AsnLys: 0.798 ± 0.021
2.597AsnLeu: 2.597 ± 0.036
0.605AsnMet: 0.605 ± 0.017
0.807AsnAsn: 0.807 ± 0.023
1.836AsnPro: 1.836 ± 0.029
0.823AsnGln: 0.823 ± 0.019
1.719AsnArg: 1.719 ± 0.025
1.384AsnSer: 1.384 ± 0.026
1.289AsnThr: 1.289 ± 0.025
2.12AsnVal: 2.12 ± 0.037
0.443AsnTrp: 0.443 ± 0.014
0.722AsnTyr: 0.722 ± 0.017
0.0AsnXaa: 0.0 ± 0.0
Pro
6.561ProAla: 6.561 ± 0.062
0.367ProCys: 0.367 ± 0.012
3.634ProAsp: 3.634 ± 0.037
3.302ProGlu: 3.302 ± 0.042
1.994ProPhe: 1.994 ± 0.029
4.391ProGly: 4.391 ± 0.046
1.142ProHis: 1.142 ± 0.023
2.523ProIle: 2.523 ± 0.035
1.844ProLys: 1.844 ± 0.029
4.873ProLeu: 4.873 ± 0.048
1.23ProMet: 1.23 ± 0.024
1.548ProAsn: 1.548 ± 0.028
2.996ProPro: 2.996 ± 0.049
1.879ProGln: 1.879 ± 0.031
3.224ProArg: 3.224 ± 0.044
3.145ProSer: 3.145 ± 0.039
2.743ProThr: 2.743 ± 0.032
4.083ProVal: 4.083 ± 0.037
0.725ProTrp: 0.725 ± 0.017
1.242ProTyr: 1.242 ± 0.026
0.0ProXaa: 0.0 ± 0.0
Gln
4.352GlnAla: 4.352 ± 0.052
0.249GlnCys: 0.249 ± 0.011
1.506GlnAsp: 1.506 ± 0.027
1.372GlnGlu: 1.372 ± 0.026
1.216GlnPhe: 1.216 ± 0.023
2.373GlnGly: 2.373 ± 0.033
0.714GlnHis: 0.714 ± 0.017
1.856GlnIle: 1.856 ± 0.03
1.075GlnLys: 1.075 ± 0.019
3.358GlnLeu: 3.358 ± 0.046
0.882GlnMet: 0.882 ± 0.019
0.887GlnAsn: 0.887 ± 0.02
1.959GlnPro: 1.959 ± 0.037
1.452GlnGln: 1.452 ± 0.03
2.608GlnArg: 2.608 ± 0.032
1.978GlnSer: 1.978 ± 0.034
1.838GlnThr: 1.838 ± 0.031
2.31GlnVal: 2.31 ± 0.035
0.458GlnTrp: 0.458 ± 0.015
0.679GlnTyr: 0.679 ± 0.017
0.0GlnXaa: 0.0 ± 0.0
Arg
8.147ArgAla: 8.147 ± 0.061
0.639ArgCys: 0.639 ± 0.018
4.078ArgAsp: 4.078 ± 0.048
4.005ArgGlu: 4.005 ± 0.045
2.817ArgPhe: 2.817 ± 0.041
4.836ArgGly: 4.836 ± 0.046
1.744ArgHis: 1.744 ± 0.031
3.964ArgIle: 3.964 ± 0.041
2.374ArgLys: 2.374 ± 0.033
7.834ArgLeu: 7.834 ± 0.055
1.818ArgMet: 1.818 ± 0.026
1.931ArgAsn: 1.931 ± 0.034
3.705ArgPro: 3.705 ± 0.046
2.551ArgGln: 2.551 ± 0.033
5.728ArgArg: 5.728 ± 0.055
4.071ArgSer: 4.071 ± 0.047
3.372ArgThr: 3.372 ± 0.038
4.67ArgVal: 4.67 ± 0.046
1.027ArgTrp: 1.027 ± 0.021
1.7ArgTyr: 1.7 ± 0.029
0.001ArgXaa: 0.001 ± 0.001
Ser
6.763SerAla: 6.763 ± 0.067
0.546SerCys: 0.546 ± 0.018
3.114SerAsp: 3.114 ± 0.034
2.906SerGlu: 2.906 ± 0.034
2.423SerPhe: 2.423 ± 0.027
5.636SerGly: 5.636 ± 0.06
1.171SerHis: 1.171 ± 0.019
2.894SerIle: 2.894 ± 0.037
1.673SerLys: 1.673 ± 0.025
5.579SerLeu: 5.579 ± 0.051
1.346SerMet: 1.346 ± 0.02
1.569SerAsn: 1.569 ± 0.027
3.014SerPro: 3.014 ± 0.042
1.739SerGln: 1.739 ± 0.028
3.846SerArg: 3.846 ± 0.038
3.513SerSer: 3.513 ± 0.059
2.866SerThr: 2.866 ± 0.039
4.051SerVal: 4.051 ± 0.047
0.845SerTrp: 0.845 ± 0.018
1.424SerTyr: 1.424 ± 0.031
0.0SerXaa: 0.0 ± 0.0
Thr
6.464ThrAla: 6.464 ± 0.063
0.462ThrCys: 0.462 ± 0.015
2.732ThrAsp: 2.732 ± 0.037
2.59ThrGlu: 2.59 ± 0.036
2.049ThrPhe: 2.049 ± 0.031
4.75ThrGly: 4.75 ± 0.049
1.027ThrHis: 1.027 ± 0.022
3.116ThrIle: 3.116 ± 0.041
1.567ThrLys: 1.567 ± 0.028
5.525ThrLeu: 5.525 ± 0.061
1.266ThrMet: 1.266 ± 0.024
1.42ThrAsn: 1.42 ± 0.027
3.27ThrPro: 3.27 ± 0.038
1.542ThrGln: 1.542 ± 0.029
3.32ThrArg: 3.32 ± 0.033
3.175ThrSer: 3.175 ± 0.041
3.083ThrThr: 3.083 ± 0.046
4.238ThrVal: 4.238 ± 0.045
0.695ThrTrp: 0.695 ± 0.018
1.242ThrTyr: 1.242 ± 0.025
0.0ThrXaa: 0.0 ± 0.0
Val
9.53ValAla: 9.53 ± 0.066
0.645ValCys: 0.645 ± 0.017
3.958ValAsp: 3.958 ± 0.042
4.039ValGlu: 4.039 ± 0.043
2.652ValPhe: 2.652 ± 0.033
5.642ValGly: 5.642 ± 0.054
1.447ValHis: 1.447 ± 0.025
4.101ValIle: 4.101 ± 0.041
2.39ValLys: 2.39 ± 0.035
7.551ValLeu: 7.551 ± 0.06
1.757ValMet: 1.757 ± 0.029
2.041ValAsn: 2.041 ± 0.029
3.865ValPro: 3.865 ± 0.041
2.203ValGln: 2.203 ± 0.034
4.933ValArg: 4.933 ± 0.046
4.366ValSer: 4.366 ± 0.049
4.499ValThr: 4.499 ± 0.048
6.218ValVal: 6.218 ± 0.057
0.89ValTrp: 0.89 ± 0.022
1.497ValTyr: 1.497 ± 0.024
0.0ValXaa: 0.0 ± 0.0
Trp
1.197TrpAla: 1.197 ± 0.023
0.146TrpCys: 0.146 ± 0.007
0.624TrpAsp: 0.624 ± 0.018
0.499TrpGlu: 0.499 ± 0.014
0.538TrpPhe: 0.538 ± 0.017
0.908TrpGly: 0.908 ± 0.021
0.368TrpHis: 0.368 ± 0.012
0.747TrpIle: 0.747 ± 0.019
0.449TrpLys: 0.449 ± 0.014
1.702TrpLeu: 1.702 ± 0.03
0.347TrpMet: 0.347 ± 0.013
0.468TrpAsn: 0.468 ± 0.014
0.721TrpPro: 0.721 ± 0.018
0.607TrpGln: 0.607 ± 0.016
1.214TrpArg: 1.214 ± 0.02
0.889TrpSer: 0.889 ± 0.02
0.809TrpThr: 0.809 ± 0.021
0.79TrpVal: 0.79 ± 0.018
0.239TrpTrp: 0.239 ± 0.011
0.308TrpTyr: 0.308 ± 0.012
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.493TyrAla: 2.493 ± 0.032
0.256TyrCys: 0.256 ± 0.011
1.429TyrAsp: 1.429 ± 0.029
1.093TyrGlu: 1.093 ± 0.022
0.949TyrPhe: 0.949 ± 0.02
2.061TyrGly: 2.061 ± 0.031
0.488TyrHis: 0.488 ± 0.014
0.917TyrIle: 0.917 ± 0.017
0.628TyrLys: 0.628 ± 0.018
2.416TyrLeu: 2.416 ± 0.036
0.414TyrMet: 0.414 ± 0.014
0.608TyrAsn: 0.608 ± 0.019
1.197TyrPro: 1.197 ± 0.024
0.82TyrGln: 0.82 ± 0.02
1.78TyrArg: 1.78 ± 0.025
1.174TyrSer: 1.174 ± 0.026
1.049TyrThr: 1.049 ± 0.022
1.739TyrVal: 1.739 ± 0.03
0.399TyrTrp: 0.399 ± 0.013
0.638TyrTyr: 0.638 ± 0.018
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.001XaaIle: 0.001 ± 0.001
0.0XaaLys: 0.0 ± 0.0
0.001XaaLeu: 0.001 ± 0.001
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.001XaaArg: 0.001 ± 0.001
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.007XaaXaa: 0.007 ± 0.006
Statistics based on 8201 proteins (2401352 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski