Penicillium roqueforti (strain FM164)
Average proteome isoelectric point is 6.52
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 12315 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|W6Q1P2|W6Q1P2_PENRF Winged helix-turn-helix transcription repressor DNA-binding OS=Penicillium roqueforti (strain FM164) OX=1365484 GN=PROQFM164_S02g000379 PE=4 SV=1
MM1 pKa = 7.37 HH2 pKa = 7.01 TQQLLPICGLLLASIAIASKK22 pKa = 10.67 LDD24 pKa = 3.69 RR25 pKa = 11.84 DD26 pKa = 4.25 DD27 pKa = 3.63 VPNRR31 pKa = 11.84 CWPACSSVVGIAKK44 pKa = 10.23 SCDD47 pKa = 2.92 AKK49 pKa = 10.87 YY50 pKa = 10.64 EE51 pKa = 4.06 RR52 pKa = 11.84 DD53 pKa = 3.54 SAEE56 pKa = 3.9 IQCICDD62 pKa = 2.92 WDD64 pKa = 3.86 AAKK67 pKa = 10.02 TEE69 pKa = 4.24 IPLCSACITQYY80 pKa = 9.21 QTDD83 pKa = 3.8 RR84 pKa = 11.84 RR85 pKa = 11.84 NHH87 pKa = 6.87 NITQHH92 pKa = 7.31 DD93 pKa = 4.75 DD94 pKa = 4.2 DD95 pKa = 6.42 NDD97 pKa = 4.98 DD98 pKa = 6.3 DD99 pKa = 6.89 DD100 pKa = 7.56 DD101 pKa = 6.87 DD102 pKa = 7.12 DD103 pKa = 6.52 NDD105 pKa = 4.91 DD106 pKa = 4.87 DD107 pKa = 5.96 NEE109 pKa = 4.19 ALDD112 pKa = 4.18 LVRR115 pKa = 11.84 SCSLTTTTYY124 pKa = 10.7 SAAATTLIGTSTSTEE139 pKa = 4.11 STSTATSSTATITDD153 pKa = 3.46 SSSTSGGSQDD163 pKa = 3.33 STSSVSSGSSDD174 pKa = 3.26 SAGASSGSASSPTPTPDD191 pKa = 2.96 AAASISAPGATSMLGFMGLMAFAWLL216 pKa = 3.69
Molecular weight: 22.33 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.675
IPC2_protein 3.77
IPC_protein 3.808
Toseland 3.567
ProMoST 3.999
Dawson 3.821
Bjellqvist 3.986
Wikipedia 3.808
Rodwell 3.63
Grimsley 3.478
Solomon 3.821
Lehninger 3.783
Nozaki 3.935
DTASelect 4.266
Thurlkill 3.643
EMBOSS 3.808
Sillero 3.935
Patrickios 1.138
IPC_peptide 3.808
IPC2_peptide 3.91
IPC2.peptide.svr19 3.824
Protein with the highest isoelectric point:
>tr|W6PR31|W6PR31_PENRF Probable transposable element OS=Penicillium roqueforti (strain FM164) OX=1365484 GN=PROQFM164_S01g000010 PE=4 SV=1
MM1 pKa = 7.84 LDD3 pKa = 3.5 LAAARR8 pKa = 11.84 SIPRR12 pKa = 11.84 AAKK15 pKa = 8.72 PKK17 pKa = 8.31 PVRR20 pKa = 11.84 RR21 pKa = 11.84 KK22 pKa = 9.33 RR23 pKa = 11.84 ARR25 pKa = 11.84 SPAPRR30 pKa = 11.84 RR31 pKa = 11.84 SQRR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 RR37 pKa = 11.84 II38 pKa = 3.4
Molecular weight: 4.45 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.438
IPC2_protein 10.935
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.173
Rodwell 12.31
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.047
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.117
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
12315
0
12315
5087075
22
6389
413.1
45.83
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.237 ± 0.021
1.291 ± 0.01
5.661 ± 0.014
6.111 ± 0.023
3.804 ± 0.017
6.642 ± 0.022
2.45 ± 0.01
5.186 ± 0.017
4.651 ± 0.019
9.003 ± 0.024
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.26 ± 0.009
3.757 ± 0.012
6.054 ± 0.023
4.035 ± 0.016
6.089 ± 0.02
8.449 ± 0.029
5.965 ± 0.019
6.062 ± 0.016
1.475 ± 0.009
2.817 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here