Lactobacillus oeni DSM 19972
Average proteome isoelectric point is 7.34
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1945 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0R1MD26|A0A0R1MD26_9LACO GtcA family membrane protein OS=Lactobacillus oeni DSM 19972 OX=1423777 GN=FD46_GL000346 PE=3 SV=1
MM1 pKa = 7.59 AVTPEE6 pKa = 4.63 DD7 pKa = 3.25 IKK9 pKa = 11.29 NSLRR13 pKa = 11.84 IDD15 pKa = 3.46 VTADD19 pKa = 2.88 DD20 pKa = 5.34 VMIQQYY26 pKa = 10.26 IDD28 pKa = 3.18 NAKK31 pKa = 10.52 AYY33 pKa = 8.94 VQEE36 pKa = 4.9 AVDD39 pKa = 4.21 SDD41 pKa = 4.0 TDD43 pKa = 3.68 LTQYY47 pKa = 8.09 TQYY50 pKa = 11.5 DD51 pKa = 3.86 FAVSLLTQFWYY62 pKa = 9.47 QNRR65 pKa = 11.84 VIDD68 pKa = 4.01 MQQTPYY74 pKa = 10.7 QVVSMIQQLRR84 pKa = 11.84 GLISS88 pKa = 3.21
Molecular weight: 10.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.733
IPC2_protein 3.872
IPC_protein 3.821
Toseland 3.592
ProMoST 4.024
Dawson 3.846
Bjellqvist 3.999
Wikipedia 3.834
Rodwell 3.643
Grimsley 3.503
Solomon 3.821
Lehninger 3.783
Nozaki 3.973
DTASelect 4.266
Thurlkill 3.681
EMBOSS 3.846
Sillero 3.948
Patrickios 1.926
IPC_peptide 3.808
IPC2_peptide 3.91
IPC2.peptide.svr19 3.844
Protein with the highest isoelectric point:
>tr|A0A0R1MCU4|A0A0R1MCU4_9LACO PfpI family intracellular protease OS=Lactobacillus oeni DSM 19972 OX=1423777 GN=FD46_GL000462 PE=3 SV=1
MM1 pKa = 7.36 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 9.69 QPKK8 pKa = 8.69 KK9 pKa = 7.77 RR10 pKa = 11.84 HH11 pKa = 4.99 RR12 pKa = 11.84 QRR14 pKa = 11.84 VHH16 pKa = 6.07 GFRR19 pKa = 11.84 KK20 pKa = 10.03 RR21 pKa = 11.84 MSTSNGRR28 pKa = 11.84 AVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 QKK37 pKa = 10.39 GRR39 pKa = 11.84 KK40 pKa = 8.52 VLSAA44 pKa = 4.05
Molecular weight: 5.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.433
IPC2_protein 11.125
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.384
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.106
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.067
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1945
0
1945
586335
44
1491
301.5
33.77
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.615 ± 0.058
0.609 ± 0.013
4.938 ± 0.047
5.99 ± 0.06
4.654 ± 0.054
6.565 ± 0.049
1.948 ± 0.022
7.367 ± 0.049
7.821 ± 0.058
10.365 ± 0.073
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.423 ± 0.026
4.888 ± 0.036
3.358 ± 0.028
4.243 ± 0.047
4.065 ± 0.039
6.23 ± 0.054
5.563 ± 0.043
6.938 ± 0.046
0.909 ± 0.017
3.51 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here