Agromyces atrinae

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Agromyces

Average proteome isoelectric point is 5.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3277 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Q2M775|A0A4Q2M775_9MICO DNA-(apurinic or apyrimidinic site) lyase OS=Agromyces atrinae OX=592376 GN=ESP50_13255 PE=3 SV=1
MM1 pKa = 7.18PQFRR5 pKa = 11.84NHH7 pKa = 5.26MRR9 pKa = 11.84SAGVVTAIAVAGMTLAGCASSSEE32 pKa = 4.24STEE35 pKa = 3.84AGGEE39 pKa = 4.17KK40 pKa = 8.25ITYY43 pKa = 7.77WLWDD47 pKa = 3.71TNQQPAYY54 pKa = 6.96TQCATAFTEE63 pKa = 4.06KK64 pKa = 10.14TGIDD68 pKa = 3.6VTIEE72 pKa = 3.72QYY74 pKa = 10.81GWADD78 pKa = 3.15YY79 pKa = 9.36WQGLTTGFAAGTAPDD94 pKa = 3.68VFADD98 pKa = 3.48HH99 pKa = 7.13LSYY102 pKa = 11.07YY103 pKa = 9.22PQFVSQGQLLDD114 pKa = 3.21ISDD117 pKa = 5.27RR118 pKa = 11.84IDD120 pKa = 3.58ADD122 pKa = 4.6GIDD125 pKa = 3.57LSIYY129 pKa = 10.29QDD131 pKa = 3.43GLADD135 pKa = 3.8LWVDD139 pKa = 3.72QEE141 pKa = 4.16GGRR144 pKa = 11.84YY145 pKa = 9.09GLPKK149 pKa = 10.9DD150 pKa = 3.91FDD152 pKa = 4.36TVALFYY158 pKa = 11.12NEE160 pKa = 4.12TMVTDD165 pKa = 4.22AGIDD169 pKa = 3.49PAALSEE175 pKa = 4.31AAWNPTDD182 pKa = 4.47GGTFEE187 pKa = 4.17QAVAALTVDD196 pKa = 3.63EE197 pKa = 4.56NGVRR201 pKa = 11.84GTEE204 pKa = 4.16AGFDD208 pKa = 3.72KK209 pKa = 11.43NNVAVYY215 pKa = 10.39GLALSGNGLNASGQQTWAPYY235 pKa = 11.08ALGNDD240 pKa = 3.62WYY242 pKa = 11.09FGDD245 pKa = 3.77TTPWTTEE252 pKa = 3.67WNFDD256 pKa = 3.48APEE259 pKa = 4.16FTDD262 pKa = 3.19TLAWYY267 pKa = 10.07KK268 pKa = 10.69SLQDD272 pKa = 3.43KK273 pKa = 11.09GYY275 pKa = 9.42MPTIDD280 pKa = 3.69IALSEE285 pKa = 4.04QDD287 pKa = 3.98PLNGYY292 pKa = 9.07LAGRR296 pKa = 11.84YY297 pKa = 9.5ALVTDD302 pKa = 4.86GSWMNSSYY310 pKa = 10.91LGQSDD315 pKa = 4.56VPTKK319 pKa = 10.49VAPTPVGPSGEE330 pKa = 4.09RR331 pKa = 11.84ASVFNGLSDD340 pKa = 5.16GIWAGTKK347 pKa = 10.53NPDD350 pKa = 4.32AAWEE354 pKa = 4.26WVSYY358 pKa = 10.12LASTDD363 pKa = 3.82CQDD366 pKa = 3.53VVADD370 pKa = 3.86AAVVFPAIKK379 pKa = 9.83TSTEE383 pKa = 3.65KK384 pKa = 10.31ATEE387 pKa = 3.81AFAAKK392 pKa = 9.72GWDD395 pKa = 3.29VSGFAVQVDD404 pKa = 4.08EE405 pKa = 4.72GTTALLPIADD415 pKa = 4.15HH416 pKa = 6.21WSEE419 pKa = 4.2INDD422 pKa = 3.54LVTANSQAFLKK433 pKa = 10.85GSVDD437 pKa = 3.4AEE439 pKa = 4.45SFTGVNDD446 pKa = 3.39QVNALFQQ453 pKa = 4.05

Molecular weight: 48.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Q2M4L5|A0A4Q2M4L5_9MICO Pyruvate dehydrogenase (Acetyl-transferring) E1 component subunit alpha OS=Agromyces atrinae OX=592376 GN=pdhA PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.56LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3277

0

3277

1105242

29

2641

337.3

36.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.423 ± 0.061

0.451 ± 0.008

6.423 ± 0.038

5.722 ± 0.039

3.293 ± 0.027

8.879 ± 0.036

1.874 ± 0.02

4.874 ± 0.037

1.771 ± 0.028

10.102 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.558 ± 0.015

1.996 ± 0.024

5.27 ± 0.029

2.376 ± 0.02

7.189 ± 0.048

6.06 ± 0.032

6.16 ± 0.039

9.104 ± 0.04

1.486 ± 0.02

1.988 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski