Streptomyces sp. BK447

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8074 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4R7IJ91|A0A4R7IJ91_9ACTN Uncharacterized protein OS=Streptomyces sp. BK447 OX=2512159 GN=EV572_11195 PE=4 SV=1
MM1 pKa = 7.95PSRR4 pKa = 11.84PLGLVGRR11 pKa = 11.84RR12 pKa = 11.84MPRR15 pKa = 11.84ITSALTLTLGAGLVAPVLLAGTAGAAVTSATAEE48 pKa = 3.86VDD50 pKa = 3.93GKK52 pKa = 11.03SVAYY56 pKa = 8.43TAASGQANEE65 pKa = 4.35VTVTASKK72 pKa = 10.95SSGLEE77 pKa = 3.78TITYY81 pKa = 10.31VIDD84 pKa = 3.89DD85 pKa = 4.46VVPITAGSGCAYY97 pKa = 9.54PSSADD102 pKa = 3.41HH103 pKa = 6.31TKK105 pKa = 10.43VSCEE109 pKa = 3.79VTTLEE114 pKa = 4.58SQDD117 pKa = 3.55PYY119 pKa = 10.6ATLRR123 pKa = 11.84MALGDD128 pKa = 3.74GDD130 pKa = 4.34DD131 pKa = 3.74TVTYY135 pKa = 10.25TNSTDD140 pKa = 3.09QAYY143 pKa = 9.88YY144 pKa = 10.07FAAIDD149 pKa = 3.91LGAGKK154 pKa = 8.5DD155 pKa = 3.68TLTEE159 pKa = 3.86ASGVQGNDD167 pKa = 3.09VNGGDD172 pKa = 4.38GDD174 pKa = 4.04DD175 pKa = 3.93TLTVGAVTVVRR186 pKa = 11.84GQGGNDD192 pKa = 3.53TIDD195 pKa = 3.46AGEE198 pKa = 4.06GTIAQGGDD206 pKa = 3.59GNDD209 pKa = 4.1TIHH212 pKa = 7.18SGGDD216 pKa = 3.19EE217 pKa = 4.18SAVDD221 pKa = 3.98GGAGDD226 pKa = 3.84DD227 pKa = 4.31VIRR230 pKa = 11.84GGAGRR235 pKa = 11.84QNLSGGDD242 pKa = 3.81GADD245 pKa = 3.48EE246 pKa = 4.88IYY248 pKa = 11.23GEE250 pKa = 4.81DD251 pKa = 4.33GNDD254 pKa = 3.24FLYY257 pKa = 10.78GGKK260 pKa = 10.39GDD262 pKa = 4.01DD263 pKa = 4.11FLYY266 pKa = 10.7GGPGNDD272 pKa = 2.66ILYY275 pKa = 10.52GNSGNDD281 pKa = 3.44TIYY284 pKa = 11.23GNSGDD289 pKa = 4.08DD290 pKa = 3.64EE291 pKa = 5.82LYY293 pKa = 10.88GGPGTDD299 pKa = 3.65TLSGGPGTNIVRR311 pKa = 11.84QDD313 pKa = 2.93

Molecular weight: 31.13 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4R7GT44|A0A4R7GT44_9ACTN TetR family transcriptional regulator OS=Streptomyces sp. BK447 OX=2512159 GN=EV572_1604 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILASRR35 pKa = 11.84RR36 pKa = 11.84SKK38 pKa = 10.75GRR40 pKa = 11.84ARR42 pKa = 11.84LSAA45 pKa = 3.91

Molecular weight: 5.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8074

0

8074

2714795

25

17883

336.2

36.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.272 ± 0.046

0.781 ± 0.009

6.022 ± 0.025

5.742 ± 0.028

2.688 ± 0.013

9.393 ± 0.027

2.351 ± 0.014

3.142 ± 0.027

2.168 ± 0.029

10.214 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.718 ± 0.013

1.834 ± 0.018

6.139 ± 0.033

2.835 ± 0.019

8.104 ± 0.029

5.222 ± 0.021

6.305 ± 0.026

8.407 ± 0.029

1.552 ± 0.012

2.112 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski