Amino acid dipepetide frequency for Streptomyces sp. BK447

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
20.039AlaAla: 20.039 ± 0.171
1.103AlaCys: 1.103 ± 0.024
8.205AlaAsp: 8.205 ± 0.07
8.646AlaGlu: 8.646 ± 0.074
3.405AlaPhe: 3.405 ± 0.038
12.446AlaGly: 12.446 ± 0.088
2.938AlaHis: 2.938 ± 0.04
3.375AlaIle: 3.375 ± 0.041
2.777AlaLys: 2.777 ± 0.047
14.353AlaLeu: 14.353 ± 0.127
2.362AlaMet: 2.362 ± 0.03
1.956AlaAsn: 1.956 ± 0.034
7.101AlaPro: 7.101 ± 0.091
3.839AlaGln: 3.839 ± 0.051
10.15AlaArg: 10.15 ± 0.078
6.115AlaSer: 6.115 ± 0.055
7.088AlaThr: 7.088 ± 0.068
12.149AlaVal: 12.149 ± 0.104
1.865AlaTrp: 1.865 ± 0.022
2.808AlaTyr: 2.808 ± 0.036
0.0AlaXaa: 0.0 ± 0.0
Cys
1.024CysAla: 1.024 ± 0.022
0.095CysCys: 0.095 ± 0.007
0.467CysAsp: 0.467 ± 0.014
0.396CysGlu: 0.396 ± 0.014
0.224CysPhe: 0.224 ± 0.011
0.955CysGly: 0.955 ± 0.023
0.203CysHis: 0.203 ± 0.009
0.159CysIle: 0.159 ± 0.008
0.115CysLys: 0.115 ± 0.007
0.724CysLeu: 0.724 ± 0.016
0.12CysMet: 0.12 ± 0.006
0.12CysAsn: 0.12 ± 0.007
0.485CysPro: 0.485 ± 0.015
0.18CysGln: 0.18 ± 0.009
0.625CysArg: 0.625 ± 0.017
0.442CysSer: 0.442 ± 0.014
0.519CysThr: 0.519 ± 0.015
0.668CysVal: 0.668 ± 0.017
0.134CysTrp: 0.134 ± 0.007
0.152CysTyr: 0.152 ± 0.008
0.0CysXaa: 0.0 ± 0.0
Asp
7.662AspAla: 7.662 ± 0.079
0.416AspCys: 0.416 ± 0.013
3.652AspAsp: 3.652 ± 0.039
3.904AspGlu: 3.904 ± 0.043
1.646AspPhe: 1.646 ± 0.025
6.362AspGly: 6.362 ± 0.062
1.441AspHis: 1.441 ± 0.022
1.975AspIle: 1.975 ± 0.027
1.215AspLys: 1.215 ± 0.026
6.133AspLeu: 6.133 ± 0.053
0.838AspMet: 0.838 ± 0.018
1.032AspAsn: 1.032 ± 0.021
4.46AspPro: 4.46 ± 0.06
1.588AspGln: 1.588 ± 0.029
4.927AspArg: 4.927 ± 0.053
2.669AspSer: 2.669 ± 0.034
3.429AspThr: 3.429 ± 0.044
4.692AspVal: 4.692 ± 0.044
1.07AspTrp: 1.07 ± 0.023
1.108AspTyr: 1.108 ± 0.022
0.0AspXaa: 0.0 ± 0.0
Glu
7.346GluAla: 7.346 ± 0.068
0.361GluCys: 0.361 ± 0.013
2.888GluAsp: 2.888 ± 0.034
3.602GluGlu: 3.602 ± 0.045
1.516GluPhe: 1.516 ± 0.024
4.503GluGly: 4.503 ± 0.046
1.545GluHis: 1.545 ± 0.023
2.213GluIle: 2.213 ± 0.036
1.489GluLys: 1.489 ± 0.031
6.714GluLeu: 6.714 ± 0.06
0.874GluMet: 0.874 ± 0.018
1.078GluAsn: 1.078 ± 0.02
3.519GluPro: 3.519 ± 0.044
2.336GluGln: 2.336 ± 0.035
5.498GluArg: 5.498 ± 0.058
2.656GluSer: 2.656 ± 0.033
2.967GluThr: 2.967 ± 0.035
4.372GluVal: 4.372 ± 0.051
0.801GluTrp: 0.801 ± 0.019
1.143GluTyr: 1.143 ± 0.025
0.0GluXaa: 0.0 ± 0.0
Phe
3.558PheAla: 3.558 ± 0.042
0.241PheCys: 0.241 ± 0.009
1.911PheAsp: 1.911 ± 0.03
1.395PheGlu: 1.395 ± 0.021
0.861PhePhe: 0.861 ± 0.022
2.916PheGly: 2.916 ± 0.036
0.639PheHis: 0.639 ± 0.017
0.704PheIle: 0.704 ± 0.02
0.481PheLys: 0.481 ± 0.015
2.57PheLeu: 2.57 ± 0.037
0.406PheMet: 0.406 ± 0.014
0.572PheAsn: 0.572 ± 0.016
1.39PhePro: 1.39 ± 0.029
0.681PheGln: 0.681 ± 0.015
1.862PheArg: 1.862 ± 0.028
1.469PheSer: 1.469 ± 0.024
2.045PheThr: 2.045 ± 0.032
2.183PheVal: 2.183 ± 0.033
0.431PheTrp: 0.431 ± 0.012
0.564PheTyr: 0.564 ± 0.017
0.0PheXaa: 0.0 ± 0.0
Gly
10.914GlyAla: 10.914 ± 0.072
0.817GlyCys: 0.817 ± 0.02
5.162GlyAsp: 5.162 ± 0.054
5.155GlyGlu: 5.155 ± 0.046
2.815GlyPhe: 2.815 ± 0.039
8.635GlyGly: 8.635 ± 0.076
2.388GlyHis: 2.388 ± 0.031
3.41GlyIle: 3.41 ± 0.04
2.394GlyLys: 2.394 ± 0.038
9.221GlyLeu: 9.221 ± 0.076
1.965GlyMet: 1.965 ± 0.031
1.834GlyAsn: 1.834 ± 0.037
5.206GlyPro: 5.206 ± 0.057
2.72GlyGln: 2.72 ± 0.036
7.77GlyArg: 7.77 ± 0.065
5.455GlySer: 5.455 ± 0.049
6.675GlyThr: 6.675 ± 0.066
7.465GlyVal: 7.465 ± 0.065
1.684GlyTrp: 1.684 ± 0.028
2.248GlyTyr: 2.248 ± 0.037
0.0GlyXaa: 0.0 ± 0.0
His
2.79HisAla: 2.79 ± 0.04
0.215HisCys: 0.215 ± 0.009
1.368HisAsp: 1.368 ± 0.024
1.281HisGlu: 1.281 ± 0.023
0.627HisPhe: 0.627 ± 0.016
2.457HisGly: 2.457 ± 0.034
0.712HisHis: 0.712 ± 0.019
0.697HisIle: 0.697 ± 0.019
0.355HisLys: 0.355 ± 0.009
2.493HisLeu: 2.493 ± 0.032
0.339HisMet: 0.339 ± 0.012
0.39HisAsn: 0.39 ± 0.013
1.839HisPro: 1.839 ± 0.028
0.7HisGln: 0.7 ± 0.018
2.148HisArg: 2.148 ± 0.033
1.04HisSer: 1.04 ± 0.021
1.43HisThr: 1.43 ± 0.023
1.779HisVal: 1.779 ± 0.027
0.37HisTrp: 0.37 ± 0.013
0.476HisTyr: 0.476 ± 0.015
0.0HisXaa: 0.0 ± 0.0
Ile
4.583IleAla: 4.583 ± 0.051
0.278IleCys: 0.278 ± 0.011
2.257IleAsp: 2.257 ± 0.033
2.014IleGlu: 2.014 ± 0.031
0.653IlePhe: 0.653 ± 0.019
3.421IleGly: 3.421 ± 0.044
0.621IleHis: 0.621 ± 0.017
0.86IleIle: 0.86 ± 0.019
0.737IleLys: 0.737 ± 0.02
2.353IleLeu: 2.353 ± 0.034
0.431IleMet: 0.431 ± 0.012
0.707IleAsn: 0.707 ± 0.017
1.813IlePro: 1.813 ± 0.027
0.733IleGln: 0.733 ± 0.019
2.34IleArg: 2.34 ± 0.032
1.707IleSer: 1.707 ± 0.027
2.307IleThr: 2.307 ± 0.037
2.738IleVal: 2.738 ± 0.041
0.368IleTrp: 0.368 ± 0.011
0.498IleTyr: 0.498 ± 0.014
0.0IleXaa: 0.0 ± 0.0
Lys
2.963LysAla: 2.963 ± 0.048
0.111LysCys: 0.111 ± 0.007
1.375LysAsp: 1.375 ± 0.026
1.279LysGlu: 1.279 ± 0.031
0.436LysPhe: 0.436 ± 0.013
1.868LysGly: 1.868 ± 0.036
0.428LysHis: 0.428 ± 0.013
0.821LysIle: 0.821 ± 0.022
0.88LysLys: 0.88 ± 0.028
2.01LysLeu: 2.01 ± 0.031
0.381LysMet: 0.381 ± 0.013
0.544LysAsn: 0.544 ± 0.018
1.37LysPro: 1.37 ± 0.028
0.706LysGln: 0.706 ± 0.02
1.456LysArg: 1.456 ± 0.028
1.193LysSer: 1.193 ± 0.022
1.281LysThr: 1.281 ± 0.029
1.842LysVal: 1.842 ± 0.033
0.288LysTrp: 0.288 ± 0.012
0.445LysTyr: 0.445 ± 0.014
0.0LysXaa: 0.0 ± 0.0
Leu
14.597LeuAla: 14.597 ± 0.102
0.841LeuCys: 0.841 ± 0.019
6.724LeuAsp: 6.724 ± 0.068
4.601LeuGlu: 4.601 ± 0.045
2.561LeuPhe: 2.561 ± 0.031
9.068LeuGly: 9.068 ± 0.071
2.38LeuHis: 2.38 ± 0.034
3.243LeuIle: 3.243 ± 0.051
2.066LeuLys: 2.066 ± 0.038
11.073LeuLeu: 11.073 ± 0.093
1.644LeuMet: 1.644 ± 0.024
1.681LeuAsn: 1.681 ± 0.024
6.505LeuPro: 6.505 ± 0.049
2.191LeuGln: 2.191 ± 0.032
8.653LeuArg: 8.653 ± 0.071
5.411LeuSer: 5.411 ± 0.052
6.991LeuThr: 6.991 ± 0.061
8.685LeuVal: 8.685 ± 0.065
1.33LeuTrp: 1.33 ± 0.026
1.9LeuTyr: 1.9 ± 0.03
0.0LeuXaa: 0.0 ± 0.0
Met
2.264MetAla: 2.264 ± 0.028
0.133MetCys: 0.133 ± 0.008
0.918MetAsp: 0.918 ± 0.02
0.747MetGlu: 0.747 ± 0.015
0.438MetPhe: 0.438 ± 0.012
1.315MetGly: 1.315 ± 0.022
0.358MetHis: 0.358 ± 0.012
0.633MetIle: 0.633 ± 0.016
0.435MetLys: 0.435 ± 0.014
1.627MetLeu: 1.627 ± 0.025
0.273MetMet: 0.273 ± 0.01
0.454MetAsn: 0.454 ± 0.016
1.069MetPro: 1.069 ± 0.02
0.414MetGln: 0.414 ± 0.013
1.448MetArg: 1.448 ± 0.027
1.31MetSer: 1.31 ± 0.021
1.525MetThr: 1.525 ± 0.024
1.28MetVal: 1.28 ± 0.024
0.22MetTrp: 0.22 ± 0.009
0.32MetTyr: 0.32 ± 0.012
0.0MetXaa: 0.0 ± 0.0
Asn
2.27AsnAla: 2.27 ± 0.033
0.166AsnCys: 0.166 ± 0.008
0.992AsnAsp: 0.992 ± 0.023
0.881AsnGlu: 0.881 ± 0.018
0.48AsnPhe: 0.48 ± 0.012
2.002AsnGly: 2.002 ± 0.034
0.402AsnHis: 0.402 ± 0.013
0.649AsnIle: 0.649 ± 0.017
0.422AsnLys: 0.422 ± 0.014
1.67AsnLeu: 1.67 ± 0.027
0.302AsnMet: 0.302 ± 0.011
0.469AsnAsn: 0.469 ± 0.015
1.415AsnPro: 1.415 ± 0.028
0.569AsnGln: 0.569 ± 0.017
1.314AsnArg: 1.314 ± 0.024
1.026AsnSer: 1.026 ± 0.023
1.169AsnThr: 1.169 ± 0.025
1.384AsnVal: 1.384 ± 0.026
0.327AsnTrp: 0.327 ± 0.013
0.429AsnTyr: 0.429 ± 0.016
0.0AsnXaa: 0.0 ± 0.0
Pro
8.277ProAla: 8.277 ± 0.113
0.326ProCys: 0.326 ± 0.013
4.595ProAsp: 4.595 ± 0.057
4.471ProGlu: 4.471 ± 0.04
1.545ProPhe: 1.545 ± 0.026
6.636ProGly: 6.636 ± 0.059
1.49ProHis: 1.49 ± 0.025
1.272ProIle: 1.272 ± 0.022
1.225ProLys: 1.225 ± 0.024
5.34ProLeu: 5.34 ± 0.052
1.008ProMet: 1.008 ± 0.017
0.975ProAsn: 0.975 ± 0.022
3.622ProPro: 3.622 ± 0.061
1.838ProGln: 1.838 ± 0.037
4.078ProArg: 4.078 ± 0.047
3.437ProSer: 3.437 ± 0.045
3.348ProThr: 3.348 ± 0.035
5.466ProVal: 5.466 ± 0.054
0.961ProTrp: 0.961 ± 0.021
1.474ProTyr: 1.474 ± 0.027
0.0ProXaa: 0.0 ± 0.0
Gln
3.806GlnAla: 3.806 ± 0.052
0.168GlnCys: 0.168 ± 0.008
1.463GlnAsp: 1.463 ± 0.022
1.54GlnGlu: 1.54 ± 0.026
0.674GlnPhe: 0.674 ± 0.016
2.423GlnGly: 2.423 ± 0.035
0.685GlnHis: 0.685 ± 0.016
1.105GlnIle: 1.105 ± 0.024
0.637GlnLys: 0.637 ± 0.019
2.988GlnLeu: 2.988 ± 0.04
0.485GlnMet: 0.485 ± 0.014
0.529GlnAsn: 0.529 ± 0.015
1.8GlnPro: 1.8 ± 0.035
1.336GlnGln: 1.336 ± 0.032
2.508GlnArg: 2.508 ± 0.036
1.357GlnSer: 1.357 ± 0.025
1.386GlnThr: 1.386 ± 0.024
2.381GlnVal: 2.381 ± 0.03
0.462GlnTrp: 0.462 ± 0.014
0.621GlnTyr: 0.621 ± 0.015
0.0GlnXaa: 0.0 ± 0.0
Arg
9.786ArgAla: 9.786 ± 0.078
0.617ArgCys: 0.617 ± 0.019
4.298ArgAsp: 4.298 ± 0.041
4.768ArgGlu: 4.768 ± 0.048
2.381ArgPhe: 2.381 ± 0.028
5.897ArgGly: 5.897 ± 0.051
2.192ArgHis: 2.192 ± 0.029
3.232ArgIle: 3.232 ± 0.042
1.697ArgLys: 1.697 ± 0.032
8.919ArgLeu: 8.919 ± 0.078
1.755ArgMet: 1.755 ± 0.028
1.442ArgAsn: 1.442 ± 0.026
5.013ArgPro: 5.013 ± 0.048
2.426ArgGln: 2.426 ± 0.033
7.788ArgArg: 7.788 ± 0.067
4.128ArgSer: 4.128 ± 0.042
5.571ArgThr: 5.571 ± 0.051
5.928ArgVal: 5.928 ± 0.054
1.391ArgTrp: 1.391 ± 0.026
1.812ArgTyr: 1.812 ± 0.025
0.0ArgXaa: 0.0 ± 0.0
Ser
6.841SerAla: 6.841 ± 0.06
0.413SerCys: 0.413 ± 0.014
2.843SerAsp: 2.843 ± 0.036
2.598SerGlu: 2.598 ± 0.034
1.505SerPhe: 1.505 ± 0.024
6.272SerGly: 6.272 ± 0.062
1.094SerHis: 1.094 ± 0.019
1.417SerIle: 1.417 ± 0.026
1.056SerLys: 1.056 ± 0.025
4.922SerLeu: 4.922 ± 0.048
1.036SerMet: 1.036 ± 0.021
0.93SerAsn: 0.93 ± 0.022
3.307SerPro: 3.307 ± 0.041
1.298SerGln: 1.298 ± 0.021
3.837SerArg: 3.837 ± 0.04
3.062SerSer: 3.062 ± 0.049
3.196SerThr: 3.196 ± 0.036
4.41SerVal: 4.41 ± 0.046
0.943SerTrp: 0.943 ± 0.021
1.236SerTyr: 1.236 ± 0.023
0.0SerXaa: 0.0 ± 0.0
Thr
9.116ThrAla: 9.116 ± 0.081
0.434ThrCys: 0.434 ± 0.013
3.765ThrAsp: 3.765 ± 0.039
3.388ThrGlu: 3.388 ± 0.034
1.612ThrPhe: 1.612 ± 0.03
6.896ThrGly: 6.896 ± 0.06
1.258ThrHis: 1.258 ± 0.021
1.739ThrIle: 1.739 ± 0.034
1.202ThrLys: 1.202 ± 0.027
5.764ThrLeu: 5.764 ± 0.047
0.978ThrMet: 0.978 ± 0.019
1.084ThrAsn: 1.084 ± 0.021
4.299ThrPro: 4.299 ± 0.057
1.416ThrGln: 1.416 ± 0.026
4.073ThrArg: 4.073 ± 0.042
3.394ThrSer: 3.394 ± 0.039
4.056ThrThr: 4.056 ± 0.062
6.163ThrVal: 6.163 ± 0.057
0.952ThrTrp: 0.952 ± 0.021
1.459ThrTyr: 1.459 ± 0.028
0.0ThrXaa: 0.0 ± 0.0
Val
10.358ValAla: 10.358 ± 0.077
0.735ValCys: 0.735 ± 0.019
4.923ValAsp: 4.923 ± 0.047
4.642ValGlu: 4.642 ± 0.052
2.397ValPhe: 2.397 ± 0.035
6.366ValGly: 6.366 ± 0.063
1.979ValHis: 1.979 ± 0.029
2.885ValIle: 2.885 ± 0.036
1.708ValLys: 1.708 ± 0.029
9.461ValLeu: 9.461 ± 0.072
1.427ValMet: 1.427 ± 0.022
1.691ValAsn: 1.691 ± 0.025
5.267ValPro: 5.267 ± 0.051
2.114ValGln: 2.114 ± 0.03
7.366ValArg: 7.366 ± 0.061
4.409ValSer: 4.409 ± 0.041
5.8ValThr: 5.8 ± 0.05
7.775ValVal: 7.775 ± 0.071
1.185ValTrp: 1.185 ± 0.021
1.581ValTyr: 1.581 ± 0.026
0.0ValXaa: 0.0 ± 0.0
Trp
1.702TrpAla: 1.702 ± 0.027
0.155TrpCys: 0.155 ± 0.007
0.869TrpAsp: 0.869 ± 0.021
0.762TrpGlu: 0.762 ± 0.017
0.487TrpPhe: 0.487 ± 0.015
1.142TrpGly: 1.142 ± 0.021
0.379TrpHis: 0.379 ± 0.013
0.527TrpIle: 0.527 ± 0.015
0.382TrpLys: 0.382 ± 0.012
1.782TrpLeu: 1.782 ± 0.027
0.292TrpMet: 0.292 ± 0.009
0.416TrpAsn: 0.416 ± 0.015
0.837TrpPro: 0.837 ± 0.02
0.659TrpGln: 0.659 ± 0.015
1.353TrpArg: 1.353 ± 0.024
0.968TrpSer: 0.968 ± 0.022
1.056TrpThr: 1.056 ± 0.021
1.014TrpVal: 1.014 ± 0.021
0.351TrpTrp: 0.351 ± 0.013
0.382TrpTyr: 0.382 ± 0.013
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.826TyrAla: 2.826 ± 0.033
0.184TyrCys: 0.184 ± 0.007
1.544TyrAsp: 1.544 ± 0.028
1.35TyrGlu: 1.35 ± 0.027
0.617TyrPhe: 0.617 ± 0.016
2.353TyrGly: 2.353 ± 0.035
0.376TyrHis: 0.376 ± 0.012
0.504TyrIle: 0.504 ± 0.016
0.404TyrLys: 0.404 ± 0.013
2.026TyrLeu: 2.026 ± 0.032
0.262TyrMet: 0.262 ± 0.011
0.432TyrAsn: 0.432 ± 0.014
1.067TyrPro: 1.067 ± 0.022
0.61TyrGln: 0.61 ± 0.017
1.847TyrArg: 1.847 ± 0.026
0.967TyrSer: 0.967 ± 0.021
1.209TyrThr: 1.209 ± 0.023
1.694TyrVal: 1.694 ± 0.03
0.383TyrTrp: 0.383 ± 0.012
0.466TyrTyr: 0.466 ± 0.013
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.001XaaXaa: 0.001 ± 0.001
Statistics based on 8074 proteins (2714795 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski