Mycoplasma sp. Mirounga ES2806-GEN

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Tenericutes; Mollicutes; Mycoplasmatales; Mycoplasmataceae; Mycoplasma; unclassified Mycoplasma

Average proteome isoelectric point is 7.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 659 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6M4JFM1|A0A6M4JFM1_9MOLU S1 RNA-binding domain-containing protein OS=Mycoplasma sp. Mirounga ES2806-GEN OX=754515 GN=HLA87_01720 PE=4 SV=1
MM1 pKa = 7.68IYY3 pKa = 10.45SIDD6 pKa = 3.37LHH8 pKa = 5.99GFSVEE13 pKa = 4.05EE14 pKa = 3.77ATSRR18 pKa = 11.84ILLALQYY25 pKa = 10.97AEE27 pKa = 4.27EE28 pKa = 3.94QDD30 pKa = 3.7YY31 pKa = 10.93DD32 pKa = 3.93YY33 pKa = 11.83LDD35 pKa = 4.71IITGHH40 pKa = 5.93GTGAMKK46 pKa = 9.66VTVEE50 pKa = 3.88NLLNEE55 pKa = 4.16EE56 pKa = 4.31NYY58 pKa = 10.4DD59 pKa = 3.5YY60 pKa = 11.33SIIRR64 pKa = 11.84DD65 pKa = 3.16GCYY68 pKa = 9.26RR69 pKa = 11.84VLIYY73 pKa = 9.48GTFRR77 pKa = 4.25

Molecular weight: 8.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6M4JAZ5|A0A6M4JAZ5_9MOLU Probable GTP-binding protein EngB OS=Mycoplasma sp. Mirounga ES2806-GEN OX=754515 GN=engB PE=3 SV=1
MM1 pKa = 7.52ARR3 pKa = 11.84ILNIEE8 pKa = 3.76IPNNKK13 pKa = 8.93RR14 pKa = 11.84VVVSLTYY21 pKa = 10.34IFGIGPTRR29 pKa = 11.84AKK31 pKa = 9.96EE32 pKa = 3.75ICAKK36 pKa = 10.82ANIDD40 pKa = 3.39EE41 pKa = 4.24NVRR44 pKa = 11.84VKK46 pKa = 10.74DD47 pKa = 3.74LSEE50 pKa = 4.15EE51 pKa = 3.84QLSAIRR57 pKa = 11.84EE58 pKa = 4.17VARR61 pKa = 11.84GYY63 pKa = 7.65QTEE66 pKa = 4.33GDD68 pKa = 3.71LHH70 pKa = 8.28RR71 pKa = 11.84EE72 pKa = 3.37VSLNIKK78 pKa = 10.05RR79 pKa = 11.84LMEE82 pKa = 4.14IKK84 pKa = 10.07CYY86 pKa = 10.37RR87 pKa = 11.84GMRR90 pKa = 11.84HH91 pKa = 6.14RR92 pKa = 11.84KK93 pKa = 7.99GLPVRR98 pKa = 11.84GQSTKK103 pKa = 10.87SNARR107 pKa = 11.84TRR109 pKa = 11.84KK110 pKa = 9.46GPRR113 pKa = 11.84KK114 pKa = 7.66TVAGKK119 pKa = 10.41KK120 pKa = 9.46KK121 pKa = 10.5

Molecular weight: 13.76 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

659

0

659

239779

37

3270

363.9

41.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.674 ± 0.118

0.599 ± 0.024

5.821 ± 0.076

6.582 ± 0.088

5.089 ± 0.09

4.781 ± 0.098

1.568 ± 0.036

9.153 ± 0.096

9.867 ± 0.101

9.349 ± 0.086

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.907 ± 0.042

7.701 ± 0.127

2.737 ± 0.044

3.308 ± 0.073

2.983 ± 0.069

6.623 ± 0.062

5.485 ± 0.061

5.854 ± 0.06

0.954 ± 0.032

3.964 ± 0.063
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski