Enterococcus phage EFA-1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Studiervirinae; Kayfunavirus; unclassified Kayfunavirus

Average proteome isoelectric point is 6.64

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 51 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6M5CD08|A0A6M5CD08_9CAUD Terminase small subunit OS=Enterococcus phage EFA-1 OX=2736188 GN=EFA_00047 PE=4 SV=1
MM1 pKa = 7.21AQYY4 pKa = 10.66IPLNANDD11 pKa = 5.52DD12 pKa = 3.85LDD14 pKa = 5.55AINDD18 pKa = 3.48MLAAIGEE25 pKa = 4.33PAVLQLDD32 pKa = 3.82EE33 pKa = 5.04GNADD37 pKa = 3.04VSNAQRR43 pKa = 11.84ILHH46 pKa = 5.42RR47 pKa = 11.84VNRR50 pKa = 11.84QVQAKK55 pKa = 8.81GWNFNINEE63 pKa = 4.29AAVLTPDD70 pKa = 3.37VQDD73 pKa = 3.2NRR75 pKa = 11.84IRR77 pKa = 11.84FLPSYY82 pKa = 9.58LRR84 pKa = 11.84VMTAGATSYY93 pKa = 9.7YY94 pKa = 10.74SNMGGYY100 pKa = 10.39LYY102 pKa = 10.75DD103 pKa = 5.34LSTQSTTFTDD113 pKa = 5.45PITVEE118 pKa = 4.09LVEE121 pKa = 4.38MKK123 pKa = 9.88PFSEE127 pKa = 4.3MPVVFRR133 pKa = 11.84DD134 pKa = 3.89YY135 pKa = 11.04IVTKK139 pKa = 10.66ASRR142 pKa = 11.84EE143 pKa = 3.9FNAKK147 pKa = 10.0FFGSPEE153 pKa = 3.85SEE155 pKa = 3.98LYY157 pKa = 10.7LRR159 pKa = 11.84EE160 pKa = 4.04QEE162 pKa = 4.25AEE164 pKa = 4.21LYY166 pKa = 8.12QQVMEE171 pKa = 4.46YY172 pKa = 11.03EE173 pKa = 4.28MDD175 pKa = 3.22TGRR178 pKa = 11.84YY179 pKa = 10.29NMMSDD184 pKa = 3.43IGRR187 pKa = 11.84DD188 pKa = 3.25

Molecular weight: 21.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6M5CC05|A0A6M5CC05_9CAUD SsDNA binding protein OS=Enterococcus phage EFA-1 OX=2736188 GN=EFA_00015 PE=4 SV=1
MM1 pKa = 7.64ISALLIAIGYY11 pKa = 8.61GLIAYY16 pKa = 8.18VLVRR20 pKa = 11.84DD21 pKa = 3.75INKK24 pKa = 9.37ACKK27 pKa = 9.53VYY29 pKa = 10.38KK30 pKa = 10.33FNYY33 pKa = 8.4VRR35 pKa = 11.84LGRR38 pKa = 11.84WTVRR42 pKa = 11.84QPNGRR47 pKa = 11.84FMRR50 pKa = 11.84NLANVWDD57 pKa = 4.02IATLGSKK64 pKa = 10.07LL65 pKa = 3.58

Molecular weight: 7.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

51

0

51

12163

31

1295

238.5

26.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.666 ± 0.485

1.028 ± 0.164

6.372 ± 0.215

6.701 ± 0.387

3.667 ± 0.194

7.909 ± 0.324

1.817 ± 0.185

4.917 ± 0.209

6.52 ± 0.369

7.983 ± 0.234

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.836 ± 0.185

4.292 ± 0.24

3.667 ± 0.207

3.848 ± 0.357

5.443 ± 0.24

6.454 ± 0.351

5.599 ± 0.251

7.26 ± 0.284

1.463 ± 0.162

3.56 ± 0.15
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski