Amino acid dipepetide frequency for Enterococcus phage EFA-1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.291AlaAla: 9.291 ± 1.145
0.904AlaCys: 0.904 ± 0.276
5.591AlaAsp: 5.591 ± 0.695
5.673AlaGlu: 5.673 ± 0.803
3.289AlaPhe: 3.289 ± 0.563
7.318AlaGly: 7.318 ± 1.212
1.069AlaHis: 1.069 ± 0.336
5.591AlaIle: 5.591 ± 0.838
5.673AlaLys: 5.673 ± 0.754
6.742AlaLeu: 6.742 ± 0.984
2.384AlaMet: 2.384 ± 0.489
3.618AlaAsn: 3.618 ± 0.419
2.796AlaPro: 2.796 ± 0.601
3.124AlaGln: 3.124 ± 0.541
4.111AlaArg: 4.111 ± 0.469
5.427AlaSer: 5.427 ± 0.603
4.522AlaThr: 4.522 ± 0.815
5.427AlaVal: 5.427 ± 1.15
1.398AlaTrp: 1.398 ± 0.324
2.713AlaTyr: 2.713 ± 0.55
0.0AlaXaa: 0.0 ± 0.0
Cys
0.658CysAla: 0.658 ± 0.239
0.082CysCys: 0.082 ± 0.088
0.904CysAsp: 0.904 ± 0.382
0.904CysGlu: 0.904 ± 0.253
0.658CysPhe: 0.658 ± 0.26
0.493CysGly: 0.493 ± 0.198
0.164CysHis: 0.164 ± 0.114
0.493CysIle: 0.493 ± 0.267
0.74CysLys: 0.74 ± 0.255
0.987CysLeu: 0.987 ± 0.328
0.329CysMet: 0.329 ± 0.234
0.411CysAsn: 0.411 ± 0.199
0.411CysPro: 0.411 ± 0.181
0.329CysGln: 0.329 ± 0.188
0.493CysArg: 0.493 ± 0.238
0.658CysSer: 0.658 ± 0.239
0.329CysThr: 0.329 ± 0.163
0.658CysVal: 0.658 ± 0.261
0.164CysTrp: 0.164 ± 0.105
0.411CysTyr: 0.411 ± 0.207
0.0CysXaa: 0.0 ± 0.0
Asp
5.838AspAla: 5.838 ± 0.637
0.74AspCys: 0.74 ± 0.326
4.111AspAsp: 4.111 ± 0.644
3.864AspGlu: 3.864 ± 0.47
2.549AspPhe: 2.549 ± 0.554
6.413AspGly: 6.413 ± 0.718
1.316AspHis: 1.316 ± 0.303
3.453AspIle: 3.453 ± 0.38
3.371AspLys: 3.371 ± 0.629
5.262AspLeu: 5.262 ± 0.638
2.056AspMet: 2.056 ± 0.368
2.384AspAsn: 2.384 ± 0.561
3.124AspPro: 3.124 ± 0.602
1.973AspGln: 1.973 ± 0.43
1.973AspArg: 1.973 ± 0.405
3.618AspSer: 3.618 ± 0.509
4.193AspThr: 4.193 ± 0.571
4.44AspVal: 4.44 ± 0.599
1.069AspTrp: 1.069 ± 0.382
1.973AspTyr: 1.973 ± 0.405
0.0AspXaa: 0.0 ± 0.0
Glu
6.331GluAla: 6.331 ± 1.044
0.74GluCys: 0.74 ± 0.275
5.016GluAsp: 5.016 ± 0.659
4.522GluGlu: 4.522 ± 0.728
2.796GluPhe: 2.796 ± 0.458
4.851GluGly: 4.851 ± 0.855
1.398GluHis: 1.398 ± 0.334
2.631GluIle: 2.631 ± 0.353
3.042GluLys: 3.042 ± 0.437
5.591GluLeu: 5.591 ± 0.753
2.138GluMet: 2.138 ± 0.458
2.549GluAsn: 2.549 ± 0.544
2.384GluPro: 2.384 ± 0.395
3.207GluGln: 3.207 ± 0.509
4.111GluArg: 4.111 ± 0.522
3.947GluSer: 3.947 ± 0.662
3.371GluThr: 3.371 ± 0.441
4.276GluVal: 4.276 ± 0.652
1.316GluTrp: 1.316 ± 0.289
2.796GluTyr: 2.796 ± 0.457
0.0GluXaa: 0.0 ± 0.0
Phe
3.207PheAla: 3.207 ± 0.452
0.411PheCys: 0.411 ± 0.208
3.042PheAsp: 3.042 ± 0.559
1.727PheGlu: 1.727 ± 0.314
1.151PhePhe: 1.151 ± 0.313
2.22PheGly: 2.22 ± 0.56
0.987PheHis: 0.987 ± 0.304
1.809PheIle: 1.809 ± 0.476
3.289PheLys: 3.289 ± 0.563
2.96PheLeu: 2.96 ± 0.429
0.987PheMet: 0.987 ± 0.369
1.809PheAsn: 1.809 ± 0.379
1.316PhePro: 1.316 ± 0.342
0.987PheGln: 0.987 ± 0.267
1.562PheArg: 1.562 ± 0.315
2.384PheSer: 2.384 ± 0.428
2.22PheThr: 2.22 ± 0.3
2.631PheVal: 2.631 ± 0.495
0.493PheTrp: 0.493 ± 0.176
1.48PheTyr: 1.48 ± 0.287
0.0PheXaa: 0.0 ± 0.0
Gly
6.66GlyAla: 6.66 ± 0.924
0.904GlyCys: 0.904 ± 0.367
4.769GlyAsp: 4.769 ± 0.736
5.098GlyGlu: 5.098 ± 0.501
2.302GlyPhe: 2.302 ± 0.353
5.098GlyGly: 5.098 ± 0.489
1.151GlyHis: 1.151 ± 0.346
3.947GlyIle: 3.947 ± 0.596
5.591GlyLys: 5.591 ± 0.924
6.66GlyLeu: 6.66 ± 0.826
2.713GlyMet: 2.713 ± 0.503
2.384GlyAsn: 2.384 ± 0.488
1.398GlyPro: 1.398 ± 0.31
2.713GlyGln: 2.713 ± 0.427
5.673GlyArg: 5.673 ± 0.565
6.085GlySer: 6.085 ± 0.72
4.605GlyThr: 4.605 ± 0.568
5.838GlyVal: 5.838 ± 0.778
1.069GlyTrp: 1.069 ± 0.251
4.44GlyTyr: 4.44 ± 0.559
0.0GlyXaa: 0.0 ± 0.0
His
0.987HisAla: 0.987 ± 0.357
0.411HisCys: 0.411 ± 0.218
1.233HisAsp: 1.233 ± 0.51
1.233HisGlu: 1.233 ± 0.355
0.74HisPhe: 0.74 ± 0.253
1.069HisGly: 1.069 ± 0.292
0.247HisHis: 0.247 ± 0.146
1.151HisIle: 1.151 ± 0.219
1.562HisLys: 1.562 ± 0.345
2.22HisLeu: 2.22 ± 0.427
0.329HisMet: 0.329 ± 0.172
0.576HisAsn: 0.576 ± 0.19
0.411HisPro: 0.411 ± 0.189
0.411HisGln: 0.411 ± 0.166
1.316HisArg: 1.316 ± 0.4
0.822HisSer: 0.822 ± 0.191
1.316HisThr: 1.316 ± 0.272
1.316HisVal: 1.316 ± 0.295
0.247HisTrp: 0.247 ± 0.131
0.493HisTyr: 0.493 ± 0.215
0.0HisXaa: 0.0 ± 0.0
Ile
3.536IleAla: 3.536 ± 0.593
0.822IleCys: 0.822 ± 0.256
2.713IleAsp: 2.713 ± 0.368
3.207IleGlu: 3.207 ± 0.382
0.987IlePhe: 0.987 ± 0.283
4.769IleGly: 4.769 ± 0.548
1.151IleHis: 1.151 ± 0.32
1.891IleIle: 1.891 ± 0.394
4.029IleLys: 4.029 ± 0.532
3.042IleLeu: 3.042 ± 0.53
1.151IleMet: 1.151 ± 0.26
2.631IleAsn: 2.631 ± 0.479
2.22IlePro: 2.22 ± 0.569
1.316IleGln: 1.316 ± 0.423
3.124IleArg: 3.124 ± 0.585
3.124IleSer: 3.124 ± 0.533
3.124IleThr: 3.124 ± 0.466
4.522IleVal: 4.522 ± 0.508
0.493IleTrp: 0.493 ± 0.201
1.316IleTyr: 1.316 ± 0.242
0.0IleXaa: 0.0 ± 0.0
Lys
7.318LysAla: 7.318 ± 0.998
0.658LysCys: 0.658 ± 0.258
3.947LysAsp: 3.947 ± 0.467
3.453LysGlu: 3.453 ± 0.366
2.22LysPhe: 2.22 ± 0.473
3.782LysGly: 3.782 ± 0.556
1.316LysHis: 1.316 ± 0.396
2.302LysIle: 2.302 ± 0.348
4.111LysLys: 4.111 ± 0.862
5.591LysLeu: 5.591 ± 0.546
1.891LysMet: 1.891 ± 0.308
1.973LysAsn: 1.973 ± 0.425
2.631LysPro: 2.631 ± 0.648
1.809LysGln: 1.809 ± 0.379
4.276LysArg: 4.276 ± 0.674
4.933LysSer: 4.933 ± 0.591
3.947LysThr: 3.947 ± 0.565
5.345LysVal: 5.345 ± 0.688
1.316LysTrp: 1.316 ± 0.398
2.384LysTyr: 2.384 ± 0.368
0.0LysXaa: 0.0 ± 0.0
Leu
7.071LeuAla: 7.071 ± 0.804
0.329LeuCys: 0.329 ± 0.155
4.44LeuAsp: 4.44 ± 0.401
6.167LeuGlu: 6.167 ± 0.876
2.056LeuPhe: 2.056 ± 0.472
4.933LeuGly: 4.933 ± 0.743
0.904LeuHis: 0.904 ± 0.258
3.864LeuIle: 3.864 ± 0.625
6.66LeuLys: 6.66 ± 0.822
4.851LeuLeu: 4.851 ± 0.554
2.878LeuMet: 2.878 ± 0.552
5.18LeuAsn: 5.18 ± 0.744
3.453LeuPro: 3.453 ± 0.384
3.7LeuGln: 3.7 ± 0.543
4.687LeuArg: 4.687 ± 0.38
5.509LeuSer: 5.509 ± 0.649
4.933LeuThr: 4.933 ± 0.692
5.345LeuVal: 5.345 ± 0.724
0.74LeuTrp: 0.74 ± 0.238
2.138LeuTyr: 2.138 ± 0.543
0.0LeuXaa: 0.0 ± 0.0
Met
3.042MetAla: 3.042 ± 0.569
0.411MetCys: 0.411 ± 0.206
1.398MetAsp: 1.398 ± 0.335
2.138MetGlu: 2.138 ± 0.423
1.562MetPhe: 1.562 ± 0.349
2.384MetGly: 2.384 ± 0.426
0.411MetHis: 0.411 ± 0.158
1.48MetIle: 1.48 ± 0.295
1.233MetLys: 1.233 ± 0.27
2.302MetLeu: 2.302 ± 0.431
0.576MetMet: 0.576 ± 0.205
0.987MetAsn: 0.987 ± 0.3
0.904MetPro: 0.904 ± 0.315
0.822MetGln: 0.822 ± 0.326
1.151MetArg: 1.151 ± 0.292
1.891MetSer: 1.891 ± 0.326
1.727MetThr: 1.727 ± 0.454
2.467MetVal: 2.467 ± 0.513
0.329MetTrp: 0.329 ± 0.149
1.151MetTyr: 1.151 ± 0.279
0.0MetXaa: 0.0 ± 0.0
Asn
4.193AsnAla: 4.193 ± 0.615
0.576AsnCys: 0.576 ± 0.245
2.384AsnAsp: 2.384 ± 0.411
2.384AsnGlu: 2.384 ± 0.404
1.316AsnPhe: 1.316 ± 0.257
4.687AsnGly: 4.687 ± 0.614
0.74AsnHis: 0.74 ± 0.228
2.302AsnIle: 2.302 ± 0.336
2.384AsnLys: 2.384 ± 0.475
3.371AsnLeu: 3.371 ± 0.627
1.069AsnMet: 1.069 ± 0.338
1.727AsnAsn: 1.727 ± 0.356
2.713AsnPro: 2.713 ± 0.473
1.48AsnGln: 1.48 ± 0.312
2.384AsnArg: 2.384 ± 0.571
2.549AsnSer: 2.549 ± 0.586
1.891AsnThr: 1.891 ± 0.389
2.96AsnVal: 2.96 ± 0.546
0.164AsnTrp: 0.164 ± 0.118
1.644AsnTyr: 1.644 ± 0.384
0.0AsnXaa: 0.0 ± 0.0
Pro
3.124ProAla: 3.124 ± 0.677
0.329ProCys: 0.329 ± 0.217
2.467ProAsp: 2.467 ± 0.342
2.796ProGlu: 2.796 ± 0.636
1.151ProPhe: 1.151 ± 0.252
1.562ProGly: 1.562 ± 0.32
0.658ProHis: 0.658 ± 0.191
2.138ProIle: 2.138 ± 0.318
3.124ProLys: 3.124 ± 0.513
2.056ProLeu: 2.056 ± 0.351
0.904ProMet: 0.904 ± 0.308
2.138ProAsn: 2.138 ± 0.469
0.658ProPro: 0.658 ± 0.204
1.562ProGln: 1.562 ± 0.368
1.48ProArg: 1.48 ± 0.379
3.042ProSer: 3.042 ± 0.521
2.796ProThr: 2.796 ± 0.374
3.289ProVal: 3.289 ± 0.336
0.658ProTrp: 0.658 ± 0.245
0.74ProTyr: 0.74 ± 0.197
0.0ProXaa: 0.0 ± 0.0
Gln
3.7GlnAla: 3.7 ± 0.555
0.164GlnCys: 0.164 ± 0.133
3.207GlnAsp: 3.207 ± 0.667
2.631GlnGlu: 2.631 ± 0.448
1.973GlnPhe: 1.973 ± 0.417
2.713GlnGly: 2.713 ± 0.562
0.493GlnHis: 0.493 ± 0.217
1.151GlnIle: 1.151 ± 0.24
1.809GlnLys: 1.809 ± 0.34
3.864GlnLeu: 3.864 ± 0.702
1.316GlnMet: 1.316 ± 0.367
1.233GlnAsn: 1.233 ± 0.358
1.316GlnPro: 1.316 ± 0.382
1.809GlnGln: 1.809 ± 0.537
2.302GlnArg: 2.302 ± 0.624
2.96GlnSer: 2.96 ± 0.47
1.809GlnThr: 1.809 ± 0.357
2.384GlnVal: 2.384 ± 0.366
0.74GlnTrp: 0.74 ± 0.256
0.904GlnTyr: 0.904 ± 0.335
0.0GlnXaa: 0.0 ± 0.0
Arg
4.193ArgAla: 4.193 ± 0.775
0.493ArgCys: 0.493 ± 0.222
4.276ArgAsp: 4.276 ± 0.44
4.111ArgGlu: 4.111 ± 0.554
2.878ArgPhe: 2.878 ± 0.378
4.44ArgGly: 4.44 ± 0.482
0.658ArgHis: 0.658 ± 0.286
3.289ArgIle: 3.289 ± 0.653
3.371ArgLys: 3.371 ± 0.634
5.591ArgLeu: 5.591 ± 0.756
1.151ArgMet: 1.151 ± 0.356
2.302ArgAsn: 2.302 ± 0.429
1.644ArgPro: 1.644 ± 0.359
2.713ArgGln: 2.713 ± 0.42
2.138ArgArg: 2.138 ± 0.383
3.289ArgSer: 3.289 ± 0.651
2.056ArgThr: 2.056 ± 0.342
2.96ArgVal: 2.96 ± 0.568
1.069ArgTrp: 1.069 ± 0.315
1.809ArgTyr: 1.809 ± 0.324
0.0ArgXaa: 0.0 ± 0.0
Ser
4.193SerAla: 4.193 ± 0.56
0.822SerCys: 0.822 ± 0.349
4.605SerAsp: 4.605 ± 0.475
4.193SerGlu: 4.193 ± 0.8
2.878SerPhe: 2.878 ± 0.365
6.825SerGly: 6.825 ± 0.948
2.467SerHis: 2.467 ± 0.433
2.96SerIle: 2.96 ± 0.576
3.864SerLys: 3.864 ± 0.555
3.536SerLeu: 3.536 ± 0.468
1.316SerMet: 1.316 ± 0.36
3.289SerAsn: 3.289 ± 0.451
2.631SerPro: 2.631 ± 0.422
2.384SerGln: 2.384 ± 0.499
3.042SerArg: 3.042 ± 0.473
3.782SerSer: 3.782 ± 0.581
3.289SerThr: 3.289 ± 0.582
4.358SerVal: 4.358 ± 0.48
1.069SerTrp: 1.069 ± 0.374
3.042SerTyr: 3.042 ± 0.549
0.0SerXaa: 0.0 ± 0.0
Thr
3.864ThrAla: 3.864 ± 0.789
0.247ThrCys: 0.247 ± 0.155
2.96ThrAsp: 2.96 ± 0.521
5.098ThrGlu: 5.098 ± 0.667
2.22ThrPhe: 2.22 ± 0.324
5.262ThrGly: 5.262 ± 0.593
0.822ThrHis: 0.822 ± 0.253
3.7ThrIle: 3.7 ± 0.631
2.96ThrLys: 2.96 ± 0.493
5.18ThrLeu: 5.18 ± 0.604
1.727ThrMet: 1.727 ± 0.376
1.891ThrAsn: 1.891 ± 0.375
2.467ThrPro: 2.467 ± 0.351
2.878ThrGln: 2.878 ± 0.526
2.302ThrArg: 2.302 ± 0.434
2.302ThrSer: 2.302 ± 0.478
2.96ThrThr: 2.96 ± 0.548
4.769ThrVal: 4.769 ± 0.626
0.904ThrTrp: 0.904 ± 0.254
1.48ThrTyr: 1.48 ± 0.251
0.0ThrXaa: 0.0 ± 0.0
Val
5.345ValAla: 5.345 ± 0.623
0.658ValCys: 0.658 ± 0.24
3.618ValAsp: 3.618 ± 0.577
5.098ValGlu: 5.098 ± 0.725
2.302ValPhe: 2.302 ± 0.51
6.085ValGly: 6.085 ± 0.617
1.069ValHis: 1.069 ± 0.532
3.207ValIle: 3.207 ± 0.533
5.18ValLys: 5.18 ± 0.733
5.509ValLeu: 5.509 ± 0.78
2.138ValMet: 2.138 ± 0.528
3.371ValAsn: 3.371 ± 0.627
2.713ValPro: 2.713 ± 0.544
3.042ValGln: 3.042 ± 0.56
4.851ValArg: 4.851 ± 0.674
5.262ValSer: 5.262 ± 0.596
3.947ValThr: 3.947 ± 0.574
6.002ValVal: 6.002 ± 0.91
0.74ValTrp: 0.74 ± 0.274
2.467ValTyr: 2.467 ± 0.436
0.0ValXaa: 0.0 ± 0.0
Trp
0.493TrpAla: 0.493 ± 0.143
0.082TrpCys: 0.082 ± 0.081
0.822TrpAsp: 0.822 ± 0.229
0.904TrpGlu: 0.904 ± 0.248
0.493TrpPhe: 0.493 ± 0.19
1.151TrpGly: 1.151 ± 0.289
0.411TrpHis: 0.411 ± 0.198
0.247TrpIle: 0.247 ± 0.144
1.151TrpLys: 1.151 ± 0.38
2.138TrpLeu: 2.138 ± 0.441
0.164TrpMet: 0.164 ± 0.116
0.904TrpAsn: 0.904 ± 0.247
0.411TrpPro: 0.411 ± 0.17
0.576TrpGln: 0.576 ± 0.227
0.904TrpArg: 0.904 ± 0.241
1.151TrpSer: 1.151 ± 0.507
0.74TrpThr: 0.74 ± 0.201
1.069TrpVal: 1.069 ± 0.345
0.247TrpTrp: 0.247 ± 0.151
0.576TrpTyr: 0.576 ± 0.209
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.618TyrAla: 3.618 ± 0.561
0.493TyrCys: 0.493 ± 0.214
2.22TyrAsp: 2.22 ± 0.315
1.809TyrGlu: 1.809 ± 0.388
1.151TyrPhe: 1.151 ± 0.27
3.042TyrGly: 3.042 ± 0.482
0.74TyrHis: 0.74 ± 0.242
1.562TyrIle: 1.562 ± 0.451
2.056TyrLys: 2.056 ± 0.447
2.384TyrLeu: 2.384 ± 0.424
1.069TyrMet: 1.069 ± 0.268
1.562TyrAsn: 1.562 ± 0.366
1.069TyrPro: 1.069 ± 0.416
1.644TyrGln: 1.644 ± 0.487
2.549TyrArg: 2.549 ± 0.463
1.809TyrSer: 1.809 ± 0.319
2.22TyrThr: 2.22 ± 0.384
2.549TyrVal: 2.549 ± 0.548
0.411TyrTrp: 0.411 ± 0.152
1.644TyrTyr: 1.644 ± 0.383
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 51 proteins (12163 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski