Porcine reproductive and respiratory syndrome virus (strain Lelystad) (PRRSV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Nidovirales; Arnidovirineae; Arteriviridae; Variarterivirinae; Betaarterivirus; Eurpobartevirus; Betaarterivirus suid 1; Porcine reproductive and respiratory syndrome virus 1

Average proteome isoelectric point is 7.66

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q04569|GP5_PRRSL Glycoprotein 5 OS=Porcine reproductive and respiratory syndrome virus (strain Lelystad) OX=11049 GN=GP5 PE=1 SV=1
MM1 pKa = 7.52AAATLFFLAGAQHH14 pKa = 6.09IMVSEE19 pKa = 4.3AFACKK24 pKa = 9.81PCFSTHH30 pKa = 6.63LSDD33 pKa = 5.1IEE35 pKa = 4.57TNTTAAAGFMVLQDD49 pKa = 3.42INCFRR54 pKa = 11.84PHH56 pKa = 6.1GVSAAQEE63 pKa = 4.18KK64 pKa = 10.51ISFGKK69 pKa = 10.43SSQCRR74 pKa = 11.84EE75 pKa = 4.07AVGTPQYY82 pKa = 10.07ITITANVTDD91 pKa = 4.07EE92 pKa = 4.52SYY94 pKa = 11.16LYY96 pKa = 10.94NADD99 pKa = 4.24LLMLSACLFYY109 pKa = 10.88ASEE112 pKa = 4.12MSEE115 pKa = 4.0KK116 pKa = 10.14GFKK119 pKa = 10.72VIFGNVSGVVSACVNFTDD137 pKa = 4.45YY138 pKa = 10.78VAHH141 pKa = 5.73VTQHH145 pKa = 5.01TQQHH149 pKa = 6.36HH150 pKa = 5.87LVIDD154 pKa = 4.64HH155 pKa = 6.7IRR157 pKa = 11.84LLHH160 pKa = 6.26FLTPSAMRR168 pKa = 11.84WATTIACLFAILLAII183 pKa = 4.72

Molecular weight: 19.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q04566|GP2A_PRRSL Glycoprotein 2a OS=Porcine reproductive and respiratory syndrome virus (strain Lelystad) OX=11049 GN=GP2a PE=1 SV=1
MM1 pKa = 7.6GGLDD5 pKa = 4.73DD6 pKa = 5.03FCNDD10 pKa = 4.64PIAAQKK16 pKa = 10.6LVLAFSITYY25 pKa = 7.79TPIMIYY31 pKa = 10.21ALKK34 pKa = 10.28VSRR37 pKa = 11.84GRR39 pKa = 11.84LLGLLHH45 pKa = 7.33ILIFLNCSFTFGYY58 pKa = 6.87MTYY61 pKa = 10.53VHH63 pKa = 6.61FQSTNRR69 pKa = 11.84VALTLGAVVALLWGVYY85 pKa = 10.17SFTEE89 pKa = 3.64SWKK92 pKa = 10.78FITSRR97 pKa = 11.84CRR99 pKa = 11.84LCCLGRR105 pKa = 11.84RR106 pKa = 11.84YY107 pKa = 9.92ILAPAHH113 pKa = 6.09HH114 pKa = 6.83VEE116 pKa = 4.56SAAGLHH122 pKa = 6.44SISASGNRR130 pKa = 11.84AYY132 pKa = 10.44AVRR135 pKa = 11.84KK136 pKa = 9.38PGLTSVNGTLVPGLRR151 pKa = 11.84SLVLGGKK158 pKa = 9.24RR159 pKa = 11.84AVKK162 pKa = 10.05RR163 pKa = 11.84GVVNLVKK170 pKa = 10.72YY171 pKa = 10.64GRR173 pKa = 3.87

Molecular weight: 18.93 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10

1

11

8848

43

3855

804.4

88.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.544 ± 0.27

3.052 ± 0.083

4.713 ± 0.423

4.204 ± 0.31

4.509 ± 0.512

7.177 ± 0.333

2.633 ± 0.278

3.617 ± 0.347

3.922 ± 0.309

10.533 ± 0.276

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.763 ± 0.102

2.735 ± 0.085

6.939 ± 0.78

3.639 ± 0.271

5.448 ± 0.217

8.262 ± 0.493

5.741 ± 0.29

8.16 ± 0.195

1.989 ± 0.246

2.419 ± 0.26
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski