Potato virus X (strain X3) (PVX)

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Tymovirales; Alphaflexiviridae; Potexvirus; Potato virus X

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P17782|CAPSD_PVXX3 Coat protein OS=Potato virus X (strain X3) OX=12185 PE=1 SV=1
MM1 pKa = 7.55EE2 pKa = 4.6VNTYY6 pKa = 10.57LNAIILVLVVTIIAVISTSLVRR28 pKa = 11.84TEE30 pKa = 3.84PCVIKK35 pKa = 9.94ITGEE39 pKa = 4.21SITVLACKK47 pKa = 10.22LDD49 pKa = 3.61AEE51 pKa = 4.72TIKK54 pKa = 10.93AIADD58 pKa = 4.17LKK60 pKa = 9.41PLSVEE65 pKa = 3.79RR66 pKa = 11.84LSFHH70 pKa = 7.04

Molecular weight: 7.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P17779|RDRP_PVXX3 RNA replication protein OS=Potato virus X (strain X3) OX=12185 PE=3 SV=1
MM1 pKa = 7.43SAQGHH6 pKa = 6.52RR7 pKa = 11.84LTAPVNSEE15 pKa = 3.28KK16 pKa = 11.06VYY18 pKa = 10.66IVLGLSFALVSITFLLSRR36 pKa = 11.84NSLPHH41 pKa = 7.2VGDD44 pKa = 4.72NIHH47 pKa = 6.36SLPHH51 pKa = 5.52GGAYY55 pKa = 9.71RR56 pKa = 11.84DD57 pKa = 3.65GTKK60 pKa = 10.46AILYY64 pKa = 7.41NSPNLGSRR72 pKa = 11.84VSLHH76 pKa = 5.9NGKK79 pKa = 8.86NAAFAAVLLLTLLIYY94 pKa = 10.12GSKK97 pKa = 10.63YY98 pKa = 9.28ISQRR102 pKa = 11.84NHH104 pKa = 4.45TCACGNNHH112 pKa = 6.26SSHH115 pKa = 6.45

Molecular weight: 12.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

2104

70

1456

420.8

47.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.46 ± 1.696

1.521 ± 0.151

4.705 ± 0.636

7.034 ± 1.35

4.278 ± 0.333

5.418 ± 0.918

2.804 ± 0.581

5.751 ± 0.865

6.749 ± 1.03

9.03 ± 0.977

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.139 ± 0.482

3.755 ± 0.742

5.133 ± 0.322

3.802 ± 0.76

4.42 ± 0.21

6.559 ± 1.083

7.937 ± 0.903

6.131 ± 0.694

1.141 ± 0.375

3.232 ± 0.521
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski