Streptomyces sp. MBRL 10

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1749 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0D6WUT9|A0A0D6WUT9_9ACTN Hydrolase (Fragment) OS=Streptomyces sp. MBRL 10 OX=1592727 GN=SF23_04470 PE=4 SV=1
MM1 pKa = 7.5SVQQEE6 pKa = 4.48APTGGADD13 pKa = 3.6GAGEE17 pKa = 4.02PLEE20 pKa = 4.18VWIDD24 pKa = 3.44QDD26 pKa = 3.99LCTGDD31 pKa = 5.01GICAQYY37 pKa = 10.9APEE40 pKa = 4.23VFEE43 pKa = 5.9LDD45 pKa = 3.05IDD47 pKa = 3.63GLAYY51 pKa = 10.42VKK53 pKa = 10.8SADD56 pKa = 4.51DD57 pKa = 4.28EE58 pKa = 4.47LLQDD62 pKa = 4.07VGATTPVPLTLLQDD76 pKa = 3.88VVDD79 pKa = 4.41SAKK82 pKa = 10.25EE83 pKa = 3.92CPGDD87 pKa = 4.18CIHH90 pKa = 6.36VRR92 pKa = 11.84RR93 pKa = 11.84VSDD96 pKa = 3.3RR97 pKa = 11.84VEE99 pKa = 4.16VYY101 pKa = 10.89GPDD104 pKa = 3.45AEE106 pKa = 4.31

Molecular weight: 11.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0D6W3C2|A0A0D6W3C2_9ACTN Acetyltransferase OS=Streptomyces sp. MBRL 10 OX=1592727 GN=SF23_21155 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILANRR35 pKa = 11.84RR36 pKa = 11.84SKK38 pKa = 10.78GRR40 pKa = 11.84AALSAA45 pKa = 3.96

Molecular weight: 5.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1749

0

1749

321611

27

961

183.9

19.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.979 ± 0.085

0.961 ± 0.022

5.965 ± 0.046

5.964 ± 0.061

2.728 ± 0.038

9.204 ± 0.065

2.367 ± 0.03

3.141 ± 0.044

2.391 ± 0.061

9.867 ± 0.069

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.984 ± 0.031

1.765 ± 0.035

5.96 ± 0.053

2.787 ± 0.041

8.422 ± 0.071

5.365 ± 0.056

6.139 ± 0.051

8.521 ± 0.07

1.492 ± 0.031

1.997 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski