Amino acid dipepetide frequency for Streptomyces sp. MBRL 10

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
19.999AlaAla: 19.999 ± 0.341
1.088AlaCys: 1.088 ± 0.065
8.119AlaAsp: 8.119 ± 0.163
8.541AlaGlu: 8.541 ± 0.182
3.451AlaPhe: 3.451 ± 0.101
12.248AlaGly: 12.248 ± 0.204
2.923AlaHis: 2.923 ± 0.104
3.243AlaIle: 3.243 ± 0.122
3.109AlaLys: 3.109 ± 0.123
12.72AlaLeu: 12.72 ± 0.234
2.982AlaMet: 2.982 ± 0.109
1.884AlaAsn: 1.884 ± 0.079
6.517AlaPro: 6.517 ± 0.122
3.681AlaGln: 3.681 ± 0.097
10.021AlaArg: 10.021 ± 0.22
6.206AlaSer: 6.206 ± 0.149
6.654AlaThr: 6.654 ± 0.148
11.968AlaVal: 11.968 ± 0.249
1.813AlaTrp: 1.813 ± 0.082
2.618AlaTyr: 2.618 ± 0.097
0.0AlaXaa: 0.0 ± 0.0
Cys
1.219CysAla: 1.219 ± 0.066
0.143CysCys: 0.143 ± 0.02
0.541CysAsp: 0.541 ± 0.037
0.426CysGlu: 0.426 ± 0.038
0.28CysPhe: 0.28 ± 0.027
1.107CysGly: 1.107 ± 0.066
0.215CysHis: 0.215 ± 0.026
0.239CysIle: 0.239 ± 0.03
0.165CysLys: 0.165 ± 0.023
0.759CysLeu: 0.759 ± 0.048
0.174CysMet: 0.174 ± 0.027
0.155CysAsn: 0.155 ± 0.023
0.656CysPro: 0.656 ± 0.047
0.236CysGln: 0.236 ± 0.028
0.827CysArg: 0.827 ± 0.059
0.675CysSer: 0.675 ± 0.043
0.662CysThr: 0.662 ± 0.048
0.784CysVal: 0.784 ± 0.05
0.221CysTrp: 0.221 ± 0.031
0.127CysTyr: 0.127 ± 0.017
0.0CysXaa: 0.0 ± 0.0
Asp
7.434AspAla: 7.434 ± 0.159
0.566AspCys: 0.566 ± 0.04
3.283AspAsp: 3.283 ± 0.111
4.02AspGlu: 4.02 ± 0.111
1.673AspPhe: 1.673 ± 0.064
6.405AspGly: 6.405 ± 0.14
1.486AspHis: 1.486 ± 0.068
1.782AspIle: 1.782 ± 0.078
1.337AspLys: 1.337 ± 0.074
6.359AspLeu: 6.359 ± 0.153
0.92AspMet: 0.92 ± 0.055
0.917AspAsn: 0.917 ± 0.051
4.297AspPro: 4.297 ± 0.111
1.524AspGln: 1.524 ± 0.059
5.143AspArg: 5.143 ± 0.138
2.537AspSer: 2.537 ± 0.082
3.224AspThr: 3.224 ± 0.097
4.67AspVal: 4.67 ± 0.119
0.979AspTrp: 0.979 ± 0.057
1.094AspTyr: 1.094 ± 0.06
0.0AspXaa: 0.0 ± 0.0
Glu
7.627GluAla: 7.627 ± 0.156
0.463GluCys: 0.463 ± 0.037
3.255GluAsp: 3.255 ± 0.104
3.986GluGlu: 3.986 ± 0.119
1.682GluPhe: 1.682 ± 0.073
4.552GluGly: 4.552 ± 0.128
1.667GluHis: 1.667 ± 0.075
2.466GluIle: 2.466 ± 0.089
1.601GluLys: 1.601 ± 0.083
6.816GluLeu: 6.816 ± 0.152
1.007GluMet: 1.007 ± 0.052
1.014GluAsn: 1.014 ± 0.056
3.156GluPro: 3.156 ± 0.103
2.478GluGln: 2.478 ± 0.094
5.485GluArg: 5.485 ± 0.154
2.674GluSer: 2.674 ± 0.088
2.755GluThr: 2.755 ± 0.108
4.897GluVal: 4.897 ± 0.136
0.815GluTrp: 0.815 ± 0.042
1.244GluTyr: 1.244 ± 0.061
0.0GluXaa: 0.0 ± 0.0
Phe
3.408PheAla: 3.408 ± 0.108
0.358PheCys: 0.358 ± 0.032
1.937PheAsp: 1.937 ± 0.081
1.679PheGlu: 1.679 ± 0.078
0.74PhePhe: 0.74 ± 0.049
2.973PheGly: 2.973 ± 0.103
0.6PheHis: 0.6 ± 0.044
0.718PheIle: 0.718 ± 0.047
0.6PheLys: 0.6 ± 0.041
2.553PheLeu: 2.553 ± 0.093
0.46PheMet: 0.46 ± 0.04
0.473PheAsn: 0.473 ± 0.037
1.461PhePro: 1.461 ± 0.059
0.706PheGln: 0.706 ± 0.052
1.915PheArg: 1.915 ± 0.066
1.558PheSer: 1.558 ± 0.066
1.974PheThr: 1.974 ± 0.08
2.208PheVal: 2.208 ± 0.08
0.382PheTrp: 0.382 ± 0.033
0.581PheTyr: 0.581 ± 0.044
0.0PheXaa: 0.0 ± 0.0
Gly
10.099GlyAla: 10.099 ± 0.187
0.961GlyCys: 0.961 ± 0.059
5.199GlyAsp: 5.199 ± 0.148
5.37GlyGlu: 5.37 ± 0.148
2.718GlyPhe: 2.718 ± 0.091
8.436GlyGly: 8.436 ± 0.224
2.295GlyHis: 2.295 ± 0.092
3.308GlyIle: 3.308 ± 0.098
2.612GlyLys: 2.612 ± 0.107
9.058GlyLeu: 9.058 ± 0.171
2.012GlyMet: 2.012 ± 0.084
1.779GlyAsn: 1.779 ± 0.088
4.925GlyPro: 4.925 ± 0.124
2.702GlyGln: 2.702 ± 0.104
8.137GlyArg: 8.137 ± 0.17
5.603GlySer: 5.603 ± 0.135
6.001GlyThr: 6.001 ± 0.142
7.369GlyVal: 7.369 ± 0.168
1.57GlyTrp: 1.57 ± 0.073
1.89GlyTyr: 1.89 ± 0.081
0.0GlyXaa: 0.0 ± 0.0
His
2.714HisAla: 2.714 ± 0.106
0.218HisCys: 0.218 ± 0.026
1.405HisAsp: 1.405 ± 0.064
1.356HisGlu: 1.356 ± 0.066
0.678HisPhe: 0.678 ± 0.039
2.372HisGly: 2.372 ± 0.088
0.721HisHis: 0.721 ± 0.06
0.613HisIle: 0.613 ± 0.041
0.438HisLys: 0.438 ± 0.035
2.363HisLeu: 2.363 ± 0.086
0.429HisMet: 0.429 ± 0.038
0.345HisAsn: 0.345 ± 0.032
1.813HisPro: 1.813 ± 0.076
0.644HisGln: 0.644 ± 0.044
2.385HisArg: 2.385 ± 0.095
1.029HisSer: 1.029 ± 0.052
1.315HisThr: 1.315 ± 0.073
1.956HisVal: 1.956 ± 0.089
0.423HisTrp: 0.423 ± 0.035
0.454HisTyr: 0.454 ± 0.038
0.0HisXaa: 0.0 ± 0.0
Ile
4.493IleAla: 4.493 ± 0.116
0.361IleCys: 0.361 ± 0.037
2.313IleAsp: 2.313 ± 0.083
2.034IleGlu: 2.034 ± 0.077
0.647IlePhe: 0.647 ± 0.049
3.234IleGly: 3.234 ± 0.1
0.616IleHis: 0.616 ± 0.042
0.864IleIle: 0.864 ± 0.055
0.821IleLys: 0.821 ± 0.06
2.407IleLeu: 2.407 ± 0.101
0.466IleMet: 0.466 ± 0.042
0.724IleAsn: 0.724 ± 0.049
1.695IlePro: 1.695 ± 0.078
0.752IleGln: 0.752 ± 0.058
2.435IleArg: 2.435 ± 0.09
1.564IleSer: 1.564 ± 0.081
2.276IleThr: 2.276 ± 0.091
2.885IleVal: 2.885 ± 0.098
0.283IleTrp: 0.283 ± 0.033
0.541IleTyr: 0.541 ± 0.04
0.0IleXaa: 0.0 ± 0.0
Lys
3.296LysAla: 3.296 ± 0.13
0.112LysCys: 0.112 ± 0.017
1.471LysAsp: 1.471 ± 0.072
1.396LysGlu: 1.396 ± 0.07
0.544LysPhe: 0.544 ± 0.044
1.956LysGly: 1.956 ± 0.086
0.466LysHis: 0.466 ± 0.04
0.93LysIle: 0.93 ± 0.061
1.091LysLys: 1.091 ± 0.087
2.108LysLeu: 2.108 ± 0.098
0.494LysMet: 0.494 ± 0.042
0.525LysAsn: 0.525 ± 0.039
1.502LysPro: 1.502 ± 0.075
0.734LysGln: 0.734 ± 0.048
1.685LysArg: 1.685 ± 0.081
1.188LysSer: 1.188 ± 0.066
1.421LysThr: 1.421 ± 0.085
2.223LysVal: 2.223 ± 0.089
0.264LysTrp: 0.264 ± 0.03
0.504LysTyr: 0.504 ± 0.036
0.0LysXaa: 0.0 ± 0.0
Leu
13.678LeuAla: 13.678 ± 0.285
0.933LeuCys: 0.933 ± 0.057
6.816LeuAsp: 6.816 ± 0.152
4.975LeuGlu: 4.975 ± 0.143
2.631LeuPhe: 2.631 ± 0.103
8.293LeuGly: 8.293 ± 0.169
2.372LeuHis: 2.372 ± 0.088
3.016LeuIle: 3.016 ± 0.11
2.074LeuLys: 2.074 ± 0.091
10.724LeuLeu: 10.724 ± 0.229
1.807LeuMet: 1.807 ± 0.073
1.707LeuAsn: 1.707 ± 0.08
5.836LeuPro: 5.836 ± 0.129
2.13LeuGln: 2.13 ± 0.086
8.545LeuArg: 8.545 ± 0.195
5.037LeuSer: 5.037 ± 0.103
6.617LeuThr: 6.617 ± 0.157
8.523LeuVal: 8.523 ± 0.186
1.231LeuTrp: 1.231 ± 0.061
1.726LeuTyr: 1.726 ± 0.078
0.0LeuXaa: 0.0 ± 0.0
Met
2.556MetAla: 2.556 ± 0.093
0.14MetCys: 0.14 ± 0.022
1.088MetAsp: 1.088 ± 0.061
0.902MetGlu: 0.902 ± 0.049
0.445MetPhe: 0.445 ± 0.043
1.359MetGly: 1.359 ± 0.07
0.407MetHis: 0.407 ± 0.032
0.721MetIle: 0.721 ± 0.053
0.513MetLys: 0.513 ± 0.043
1.884MetLeu: 1.884 ± 0.076
0.311MetMet: 0.311 ± 0.032
0.56MetAsn: 0.56 ± 0.045
1.424MetPro: 1.424 ± 0.068
0.55MetGln: 0.55 ± 0.041
1.545MetArg: 1.545 ± 0.074
1.729MetSer: 1.729 ± 0.07
1.651MetThr: 1.651 ± 0.071
1.465MetVal: 1.465 ± 0.07
0.261MetTrp: 0.261 ± 0.023
0.326MetTyr: 0.326 ± 0.036
0.0MetXaa: 0.0 ± 0.0
Asn
2.155AsnAla: 2.155 ± 0.083
0.205AsnCys: 0.205 ± 0.025
0.942AsnAsp: 0.942 ± 0.058
0.889AsnGlu: 0.889 ± 0.053
0.426AsnPhe: 0.426 ± 0.038
1.965AsnGly: 1.965 ± 0.084
0.395AsnHis: 0.395 ± 0.034
0.644AsnIle: 0.644 ± 0.051
0.497AsnLys: 0.497 ± 0.045
1.67AsnLeu: 1.67 ± 0.071
0.348AsnMet: 0.348 ± 0.029
0.398AsnAsn: 0.398 ± 0.034
1.253AsnPro: 1.253 ± 0.06
0.504AsnGln: 0.504 ± 0.037
1.377AsnArg: 1.377 ± 0.068
0.824AsnSer: 0.824 ± 0.056
1.175AsnThr: 1.175 ± 0.054
1.287AsnVal: 1.287 ± 0.071
0.32AsnTrp: 0.32 ± 0.033
0.373AsnTyr: 0.373 ± 0.035
0.0AsnXaa: 0.0 ± 0.0
Pro
8.19ProAla: 8.19 ± 0.147
0.488ProCys: 0.488 ± 0.034
4.247ProAsp: 4.247 ± 0.123
4.297ProGlu: 4.297 ± 0.119
1.477ProPhe: 1.477 ± 0.069
6.467ProGly: 6.467 ± 0.163
1.402ProHis: 1.402 ± 0.069
1.238ProIle: 1.238 ± 0.062
1.157ProLys: 1.157 ± 0.063
4.95ProLeu: 4.95 ± 0.124
1.182ProMet: 1.182 ± 0.058
0.836ProAsn: 0.836 ± 0.056
3.454ProPro: 3.454 ± 0.118
1.673ProGln: 1.673 ± 0.082
4.167ProArg: 4.167 ± 0.124
3.523ProSer: 3.523 ± 0.129
3.116ProThr: 3.116 ± 0.1
5.578ProVal: 5.578 ± 0.134
0.787ProTrp: 0.787 ± 0.055
1.368ProTyr: 1.368 ± 0.071
0.0ProXaa: 0.0 ± 0.0
Gln
3.538GlnAla: 3.538 ± 0.116
0.243GlnCys: 0.243 ± 0.024
1.53GlnAsp: 1.53 ± 0.073
1.608GlnGlu: 1.608 ± 0.068
0.731GlnPhe: 0.731 ± 0.052
2.251GlnGly: 2.251 ± 0.089
0.746GlnHis: 0.746 ± 0.048
1.032GlnIle: 1.032 ± 0.049
0.7GlnLys: 0.7 ± 0.052
2.842GlnLeu: 2.842 ± 0.1
0.55GlnMet: 0.55 ± 0.038
0.544GlnAsn: 0.544 ± 0.046
1.604GlnPro: 1.604 ± 0.08
1.424GlnGln: 1.424 ± 0.096
2.534GlnArg: 2.534 ± 0.093
1.228GlnSer: 1.228 ± 0.061
1.415GlnThr: 1.415 ± 0.071
2.323GlnVal: 2.323 ± 0.084
0.438GlnTrp: 0.438 ± 0.038
0.585GlnTyr: 0.585 ± 0.056
0.0GlnXaa: 0.0 ± 0.0
Arg
9.232ArgAla: 9.232 ± 0.204
0.864ArgCys: 0.864 ± 0.058
4.247ArgAsp: 4.247 ± 0.12
4.944ArgGlu: 4.944 ± 0.128
2.354ArgPhe: 2.354 ± 0.091
6.396ArgGly: 6.396 ± 0.163
2.233ArgHis: 2.233 ± 0.091
3.591ArgIle: 3.591 ± 0.11
1.931ArgLys: 1.931 ± 0.075
8.644ArgLeu: 8.644 ± 0.179
2.08ArgMet: 2.08 ± 0.066
1.52ArgAsn: 1.52 ± 0.074
5.398ArgPro: 5.398 ± 0.149
2.304ArgGln: 2.304 ± 0.089
8.467ArgArg: 8.467 ± 0.224
4.81ArgSer: 4.81 ± 0.143
5.948ArgThr: 5.948 ± 0.164
5.942ArgVal: 5.942 ± 0.129
1.527ArgTrp: 1.527 ± 0.084
1.785ArgTyr: 1.785 ± 0.075
0.0ArgXaa: 0.0 ± 0.0
Ser
6.921SerAla: 6.921 ± 0.166
0.665SerCys: 0.665 ± 0.052
2.658SerAsp: 2.658 ± 0.099
2.438SerGlu: 2.438 ± 0.093
1.576SerPhe: 1.576 ± 0.079
6.169SerGly: 6.169 ± 0.172
1.045SerHis: 1.045 ± 0.055
1.433SerIle: 1.433 ± 0.062
1.138SerLys: 1.138 ± 0.062
4.698SerLeu: 4.698 ± 0.132
1.315SerMet: 1.315 ± 0.057
0.858SerAsn: 0.858 ± 0.056
3.514SerPro: 3.514 ± 0.119
1.29SerGln: 1.29 ± 0.069
4.58SerArg: 4.58 ± 0.142
3.619SerSer: 3.619 ± 0.135
3.321SerThr: 3.321 ± 0.109
4.356SerVal: 4.356 ± 0.115
0.902SerTrp: 0.902 ± 0.053
1.15SerTyr: 1.15 ± 0.061
0.0SerXaa: 0.0 ± 0.0
Thr
8.625ThrAla: 8.625 ± 0.163
0.6ThrCys: 0.6 ± 0.047
3.486ThrAsp: 3.486 ± 0.108
3.336ThrGlu: 3.336 ± 0.106
1.645ThrPhe: 1.645 ± 0.076
6.533ThrGly: 6.533 ± 0.155
1.309ThrHis: 1.309 ± 0.063
1.639ThrIle: 1.639 ± 0.077
1.349ThrLys: 1.349 ± 0.072
5.525ThrLeu: 5.525 ± 0.138
1.057ThrMet: 1.057 ± 0.056
1.014ThrAsn: 1.014 ± 0.066
4.179ThrPro: 4.179 ± 0.139
1.356ThrGln: 1.356 ± 0.063
4.366ThrArg: 4.366 ± 0.112
3.392ThrSer: 3.392 ± 0.115
3.865ThrThr: 3.865 ± 0.119
5.942ThrVal: 5.942 ± 0.146
0.895ThrTrp: 0.895 ± 0.056
1.278ThrTyr: 1.278 ± 0.062
0.0ThrXaa: 0.0 ± 0.0
Val
10.398ValAla: 10.398 ± 0.22
0.79ValCys: 0.79 ± 0.061
4.903ValAsp: 4.903 ± 0.132
5.174ValGlu: 5.174 ± 0.118
2.456ValPhe: 2.456 ± 0.09
6.225ValGly: 6.225 ± 0.15
2.068ValHis: 2.068 ± 0.089
2.932ValIle: 2.932 ± 0.112
1.971ValLys: 1.971 ± 0.088
9.219ValLeu: 9.219 ± 0.177
1.608ValMet: 1.608 ± 0.066
1.651ValAsn: 1.651 ± 0.076
5.236ValPro: 5.236 ± 0.129
2.074ValGln: 2.074 ± 0.089
7.447ValArg: 7.447 ± 0.169
4.487ValSer: 4.487 ± 0.122
5.743ValThr: 5.743 ± 0.145
8.119ValVal: 8.119 ± 0.184
1.138ValTrp: 1.138 ± 0.055
1.576ValTyr: 1.576 ± 0.066
0.0ValXaa: 0.0 ± 0.0
Trp
1.614TrpAla: 1.614 ± 0.071
0.19TrpCys: 0.19 ± 0.024
0.808TrpAsp: 0.808 ± 0.058
0.812TrpGlu: 0.812 ± 0.048
0.525TrpPhe: 0.525 ± 0.036
0.979TrpGly: 0.979 ± 0.057
0.376TrpHis: 0.376 ± 0.031
0.547TrpIle: 0.547 ± 0.045
0.426TrpLys: 0.426 ± 0.038
1.496TrpLeu: 1.496 ± 0.064
0.361TrpMet: 0.361 ± 0.029
0.373TrpAsn: 0.373 ± 0.039
0.743TrpPro: 0.743 ± 0.05
0.566TrpGln: 0.566 ± 0.043
1.306TrpArg: 1.306 ± 0.066
1.014TrpSer: 1.014 ± 0.06
1.135TrpThr: 1.135 ± 0.062
0.967TrpVal: 0.967 ± 0.055
0.336TrpTrp: 0.336 ± 0.035
0.348TrpTyr: 0.348 ± 0.036
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.59TyrAla: 2.59 ± 0.089
0.224TyrCys: 0.224 ± 0.026
1.402TyrAsp: 1.402 ± 0.076
1.458TyrGlu: 1.458 ± 0.064
0.606TyrPhe: 0.606 ± 0.044
2.124TyrGly: 2.124 ± 0.089
0.33TyrHis: 0.33 ± 0.032
0.454TyrIle: 0.454 ± 0.042
0.379TyrLys: 0.379 ± 0.037
1.878TyrLeu: 1.878 ± 0.078
0.271TyrMet: 0.271 ± 0.029
0.37TyrAsn: 0.37 ± 0.036
0.933TyrPro: 0.933 ± 0.049
0.538TyrGln: 0.538 ± 0.047
1.85TyrArg: 1.85 ± 0.082
0.951TyrSer: 0.951 ± 0.059
1.122TyrThr: 1.122 ± 0.065
1.754TyrVal: 1.754 ± 0.076
0.336TyrTrp: 0.336 ± 0.033
0.404TyrTyr: 0.404 ± 0.041
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1749 proteins (321611 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski